Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

Öz

Lysimachia nummularia L. is an important plant that has traditionally
been used in the treatment of various diseases in Europe and Asia. Due to the
valuable bioactive compounds content, this plant has a very important place in
the pharmaceutical field. In this study, the propagation of L. nummularia by tissue culture
techniques was investigated. The nodal explants of L. nummularia were cultured in Murashige and Skoog (MS) nutrient
media containing 6-benzylaminopurine (BAP) alone or in combination with
indole-3-butyric acid (IBA) at different concentrations. Generally, high rates
of shoot regeneration were obtained in both hormone trials. The maximum number
of shoots per explant (12.27) was obtained in the MS nutrient medium containing
1.6 mg L^-1 BAP, followed by MS medium containing 0.8 mg L^-1
BAP + 0.1 mg L^-1 IBA (11). The lowest number of shoots was
determined in MS medium containing 0.05 mg L^-1 BAP in both hormone
applications. The number of shoots increased with increasing BAP concentration
in the culture medium. The longest shoots were recorded in MS medium
supplemented with 0.1 mg L^-1 BAP (4.6 cm) and 0.1 mg L^-1
BAP + 0.1 mg L^-1 IBA (4.52 cm). The shortest shoot lengths were
determined in MS medium supplemented with 1.6 mg L^-1 BAP. Since the
regenerated shoots in the culture medium sustaine intensive roots, rooting
experiments have not been carried out. The rooted plants were successfully
acclimatized to the aquarium environment. As a result, the best hormone for the
propagation of L. nummularia by
tissue culture techniques was 1.6 mg L^-1 BAP. This work may help to
obtain high amounts of this valuable herbal metabolite by allowing multiple
production of L. nummularia with
tissue culture. Thus, it can be contributed to the food and pharmaceutical
sectors.