Van İlinin Çaldıran İlçesinde Yetişen Bazı Tıbbi Bitkilerin Toplam Fenolik ve Flavonoid Madde Miktarlarının Belirlenmesi
Öz
Çalışmanın amacı, Çaldıran/Van’da yetişen ve halk arasında sıkça tüketilen bazı tıbbi bitkilerin, toplam fenolik (TFM) ve flavonoid (TF) miktarlarının belirlenmesi ve farklı organlardan elde edilen su ekstrelerindeki olası farkların ortaya konmasıdır. Alcea pallida (Hiro) (Willd.) Waldst. &. Kit., Rumex scutatus L. (Evelik), Mentha longifolia (L.) L. (Dağ Reyhanı), Erigeron acris L., Achillea vermicularis Trin. (Civan Perçemi) bitkileri Van/Çaldıran'dan toplanmıştır. Ekstrelerin TFM ve TF içerikleri spektrofotometrik olarak ölçülmüştür. En yüksek TFM ve TF madde E. acris bitkisinin tohum ekstresinden elde edilmiştir. Aynı bitkinin gövde ekstresi ve M. longifolia tohum ve gövde ekstreleri de yüksek fenolik ve flavonoid madde taşıyan bitki kısımları olmuştur. Sonuçta, kullanılan beş farklı bitkiden alınan çiçek, gövde ve tohum ekstrelerinin hepsinde çiçek ve/veya tohum ekstrelerinin gövde ekstrelerine göre daha verimli olduğu ortaya konmuştur. E. acris ve M. longifolia bitkilerinden elde edilen sonuçlar, bu bitkilerin tıbbi amaçlara uygun olarak kullanılabileceğini, hem tohum hem gövde kısımlarının yoğun fenolik ve flavonoid içerdiğini göstermiştir.
Anahtar Kelimeler
Erigeron acris Fenolik madde Flavonoid Mentha longifolia Tıbbi bitki
Teşekkür
Çalışmada kullanılan bitkilerin toplanmasında ve ekstre elde edilmesi çalışmalarında yardımlarından dolayı Moleküler Biyoloji ve Genetik Bölümü 2018 mezunlarından İbrahim Çınardağ, Mazlum Taş, Rengin Özışık, Ceylan Eliş, Bahar Pelda Karadamar ve Elif Sena Şahin’e teşekkür ederiz.
Kaynakça
- Akroum S, Bendjeddou D, Satta D, Lalauoi K 2009. Antibacterial Activity and Acute Toxicity Effect of Flavonoids Entracte from Mentha longifolia. Am. Eurasian J. Sci. Res., 4, 93–96.
- Al-Rawi A, Chakravarty HL 1988. Medicinal Plants of Iraq. 65-Ministry of Agriculture and Irrigation, State Board for Agricultural and Water Resources Research, National Herbarium of Iraq, Baghdad.
- Araújo JR, Gonçalves P, Martel F 2011. Chemopreventive Effect of Dietary Polyphenols in Colorectal Cancer Cell Lines. Nutr. Res., 31, 77–87.
- Azwanida NN 2015. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Med. Aromat. Plants, 4, 1–6.
- Babaoğlu M, Ekrem G, Sebahattin Ö 2001. Bitki Biyoteknolojisi I: Doku Kültürü ve Uygulamaları. Selçuk Üniversitesi Yayınları, Konya, 374s.
- Bravo L 1998. Polyphenols: Chemistry, Dietary Sources, Metabolism and Nutritional Significance. Nutr Rev, 56, 317-333.
- Cook NC, Samman S 1996. Flavonoids: Chemistry, Metabolism, Cardioprotective Effects, and Dietary Sources. J Nutr Biochem, 7:66-76.
- Gulluce M, Sahin F, Sokmen M, Ozer H, Daferera D, Sokmen A, Polissiou M, Adiguzel A, Ozkan H 2007. Antimicrobial and Antioxidant Properties of The Essential Oils and Methanol Extract from Mentha longifolia L. sp. longifolia. Food Chem, 103, 1449–1456.
- Hosseinzadeh S, Jafarikukhdan A, Hosseini A, Armand R 2015. The Application of Medicinal Plants in Traditional and Modern Medicine: A Review of Thymus vulgaris. Int. J. Clin. Med, 6, 635–642.
- Karaman Ş, Tütem E, Sözgen BK, Apak R 2010. Comparison of Total Antioxidant Capacity and Phenolic Composition of Some Apple Juices with Combined HPLC-CUPRAC Assay. Food Chem., 120(4): 1201–1209.
- Li AN, Li S, Zhang YJ, Xu XR, Chen YM, Li HB 2014. Resources and Biological Activities of Natural Polyphenols. Nutrients, 6, 6020–6047.
- Mandlekar S, Hong JL, Kong AN 2006. Modulation of Metabolic Enzymes by Dietary Phytochemicals: A Review of Mechanisms Underlying Beneficial Versus Unfavorable Effects. Curr Drug Metab, 7(6): 661-675.
- Marquele FD, Di Mambro VM, Georgetti SR, Casagrande R, Valim YM, Fonseca MJ 2005. Assessment of the Antioxidant Activities of Brazilian Extracts of Propolis Alone and in Topical Pharmaceutical Formulations. J Pharm Biomed Anal, 39: 455-462.
- Nakamura Y, Watanabe S, Miyake N, Kohno H, Osawa T 2003. Dihydrochalcones: Evaluation as Novel Radical Scavenging Antioxidants. Journal of Agricultural and Food Chemistry, 51, 3309–3312.
- Rababah TM, Ereifej KI, Esoh RB, Al-u’datt MH, Alrababah MA, Yang W 2011. Antioxidant Activities, Total Phenolics and HPLC Analyses of The Phenolic Compounds of Extracts from Common Mediterranean plants. Nat. Prod. Res. 25, 596–605.
- Rice-Evans CA, Miller NJ, Paganga G 1996. Structure-antioxidant Activity Relationships of Flavonoids and Phenolic Acids. Free Radic. Biol. Med., 20(7): 933–956.
- Singh R 2015. Medicinal plants: A review. J Plant Sci., 3(1- 1):50-5.
- Singleton VL, Rossi, JA 1965. Colorimetry of Total Phenolics with Phosphomolybdicphosphotungstic Acid Reagents. American journal of Enology and Viticulture, 16(3), 144-158.
- Venugopal R, Liu RH 2012. Phytochemicals in Diets for Breast Cancer Prevention: The Importance of Resveratrol and Ursolic Acid. Food Sci Hum Wellness, 1: 1-13.
- Yatsyuk YK, Regal GM 1982. Patent no. SU 914061, CA 1982:428588
- Zhishen J, Mengcheng T, Jianming W 1999. The Determination of Flavonoid Contents in Mulberry and Their Scavenging Effects on Superoxide Radicals. Food chemistry, 64(4), 555-559.
Determination of Total Phenolic and Flavonoid Contents of Some Medicinal Plants Growing in Çaldıran Province of Van
Öz
The aim of the study was to determine the total phenolic (TPC) and flavonoid (TF) contents of some medicinal plants grown in Çaldıran/Van that are frequently consumed by people and to reveal possible differences in the water extracts obtained from different organs. Alcea pallida (Willd.) Waldst. &. Kit. Rumex scutatus L., Mentha longifolia (L.) L., Erigeron acris L., Achillea vermicularis Trin. were collected from Çaldıran/Van. TPC and TF contents of the extracts were measured spectrophotometrically. The highest TPC and TF were obtained from the seed extract of E. acris. Stem extracts of the same plant and the stem and seed extracts of M. longifolia were also the samples carrying high phenolic and flavonoids. As a result, it was shown that the flower and seed extracts of five different plants were more efficient than the stem extracts. Biochemical profiles of E. acris and M. longifolia showed that these plants can be used for medicinal purposes and both seed and stem parts contain notably high levels of phenolic and flavonoids
Anahtar Kelimeler
Erigeron acris Phenolics Flavonoids Mentha longifolia Medicinal plant
Kaynakça
- Akroum S, Bendjeddou D, Satta D, Lalauoi K 2009. Antibacterial Activity and Acute Toxicity Effect of Flavonoids Entracte from Mentha longifolia. Am. Eurasian J. Sci. Res., 4, 93–96.
- Al-Rawi A, Chakravarty HL 1988. Medicinal Plants of Iraq. 65-Ministry of Agriculture and Irrigation, State Board for Agricultural and Water Resources Research, National Herbarium of Iraq, Baghdad.
- Araújo JR, Gonçalves P, Martel F 2011. Chemopreventive Effect of Dietary Polyphenols in Colorectal Cancer Cell Lines. Nutr. Res., 31, 77–87.
- Azwanida NN 2015. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Med. Aromat. Plants, 4, 1–6.
- Babaoğlu M, Ekrem G, Sebahattin Ö 2001. Bitki Biyoteknolojisi I: Doku Kültürü ve Uygulamaları. Selçuk Üniversitesi Yayınları, Konya, 374s.
- Bravo L 1998. Polyphenols: Chemistry, Dietary Sources, Metabolism and Nutritional Significance. Nutr Rev, 56, 317-333.
- Cook NC, Samman S 1996. Flavonoids: Chemistry, Metabolism, Cardioprotective Effects, and Dietary Sources. J Nutr Biochem, 7:66-76.
- Gulluce M, Sahin F, Sokmen M, Ozer H, Daferera D, Sokmen A, Polissiou M, Adiguzel A, Ozkan H 2007. Antimicrobial and Antioxidant Properties of The Essential Oils and Methanol Extract from Mentha longifolia L. sp. longifolia. Food Chem, 103, 1449–1456.
- Hosseinzadeh S, Jafarikukhdan A, Hosseini A, Armand R 2015. The Application of Medicinal Plants in Traditional and Modern Medicine: A Review of Thymus vulgaris. Int. J. Clin. Med, 6, 635–642.
- Karaman Ş, Tütem E, Sözgen BK, Apak R 2010. Comparison of Total Antioxidant Capacity and Phenolic Composition of Some Apple Juices with Combined HPLC-CUPRAC Assay. Food Chem., 120(4): 1201–1209.
- Li AN, Li S, Zhang YJ, Xu XR, Chen YM, Li HB 2014. Resources and Biological Activities of Natural Polyphenols. Nutrients, 6, 6020–6047.
- Mandlekar S, Hong JL, Kong AN 2006. Modulation of Metabolic Enzymes by Dietary Phytochemicals: A Review of Mechanisms Underlying Beneficial Versus Unfavorable Effects. Curr Drug Metab, 7(6): 661-675.
- Marquele FD, Di Mambro VM, Georgetti SR, Casagrande R, Valim YM, Fonseca MJ 2005. Assessment of the Antioxidant Activities of Brazilian Extracts of Propolis Alone and in Topical Pharmaceutical Formulations. J Pharm Biomed Anal, 39: 455-462.
- Nakamura Y, Watanabe S, Miyake N, Kohno H, Osawa T 2003. Dihydrochalcones: Evaluation as Novel Radical Scavenging Antioxidants. Journal of Agricultural and Food Chemistry, 51, 3309–3312.
- Rababah TM, Ereifej KI, Esoh RB, Al-u’datt MH, Alrababah MA, Yang W 2011. Antioxidant Activities, Total Phenolics and HPLC Analyses of The Phenolic Compounds of Extracts from Common Mediterranean plants. Nat. Prod. Res. 25, 596–605.
- Rice-Evans CA, Miller NJ, Paganga G 1996. Structure-antioxidant Activity Relationships of Flavonoids and Phenolic Acids. Free Radic. Biol. Med., 20(7): 933–956.
- Singh R 2015. Medicinal plants: A review. J Plant Sci., 3(1- 1):50-5.
- Singleton VL, Rossi, JA 1965. Colorimetry of Total Phenolics with Phosphomolybdicphosphotungstic Acid Reagents. American journal of Enology and Viticulture, 16(3), 144-158.
- Venugopal R, Liu RH 2012. Phytochemicals in Diets for Breast Cancer Prevention: The Importance of Resveratrol and Ursolic Acid. Food Sci Hum Wellness, 1: 1-13.
- Yatsyuk YK, Regal GM 1982. Patent no. SU 914061, CA 1982:428588
- Zhishen J, Mengcheng T, Jianming W 1999. The Determination of Flavonoid Contents in Mulberry and Their Scavenging Effects on Superoxide Radicals. Food chemistry, 64(4), 555-559.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Yapısal Biyoloji |
| Bölüm | ARAŞTIRMA MAKALESİ (Research Article) |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Haziran 2020 |
| Gönderilme Tarihi | 30 Ekim 2019 |
| Kabul Tarihi | 6 Şubat 2020 |
| Yayımlandığı Sayı | Yıl 2020Cilt: 23 Sayı: 3 |
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2022-JCI = 0.170
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