Yıl 2020, Cilt 23 , Sayı 6, Sayfalar 1503 - 1509 2020-12-31

The Determination of In vitro Antioxidant and Cytotoxic Activities of Resin Obtained from Cilician Fir (Abies cilicica (Antoine & Kotschy) Carrière)
Toros Göknarı (Abies cilicica (Antoine & Kotschy) Carrière) ’ndan Elde Edilen Reçinenin In vitro Antioksidan ve Sitotoksik Aktivitelerinin Belirlenmesi

Esra UÇAR SÖZMEN [1] , Serap ŞAHİN BÖLÜKBAŞI [2] , Mustafa ULU [3] , Aşkın AKPOLAT [4]

In this study, the antioxidant and cytotoxic activities of resin obtained from the Cilician Fir plant were evaluated. According to data, the in vitro antioxidant activity of the resin has changed depending on the radical scavenging activity method. This resin has antioxidant activity according to 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. The in vitro cytotoxic activity of the resin was investigated against a panel of human cancer cells (MDA-MB-231, Hep G2, PC-3, U-87, MCF-7, HT-29) with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay for 48 h. Normal human lung fibroblast cells (WI-38) were used as healthy cells. The results indicated that the in vitro cytotoxic activity of the resin depends on the cell line type and concentration of the resin. According to the IC50 values, the resin has the most cytotoxic activity on endometrial adenocarcinoma cancer cells (IC50=8.94 ± 0.03 µg mL-1) compared to other cancer cells. The results also indicated that Ishikawa endometrial adenocarcinoma cells, which have Selectivity Index (SI) value >2, have the most sensitivity against the resin. This study provides the first evidence that the resin inhibits the different cancer cells' growth.
Bu çalışmada, Toros Göknarı bitkisinden elde edilen reçinenin antioksidan ve sitotoksik aktivite değerleri değerlendirilmiştir. Verilere göre, reçinenin in vitro antioksidan aktivitesi, radikal süpürücü aktivite yöntemine bağlı olarak değişmiştir. Bu reçine, 2,2′-Azino-bis- (3-etilbenzotiazolin-6-sülfonik asit) (ABTS) radikal süpürme testine göre antioksidan aktiviteye sahiptir. Reçinenin in vitro sitotoksik aktivitesi, bir dizi insan kanser hücresine (MDA-MB-231, Hep G2, PC-3, U-87, MCF-7, HT-29) karşı MTT (3- (4,5-dimetiltiyazol-2-il) -2,5-difeniltetrazolium bromid) analizi ile 48 saat boyunca araştırıldı. Sağlıklı hücreler olarak normal insan akciğer fibroblast hücreleri (WI-38) kullanıldı. Sonuçlar, reçinenin in vitro sitotoksik aktivitesinin hücre hattı tipine ve numunenin konsantrasyonuna bağlı olduğunu göstermiştir. IC50 değerlerine göre, reçine diğer kanser hücrelerine kıyasla endometriyal adenokarsinom kanser hücreleri üzerinde en fazla sitotoksik aktiviteye sahiptir (IC50 = 8.94 ± 0.03 µg mL-1). Sonuçlar ayrıca seçicilik indeks (SI) değeri> 2 olan Ishikawa endometriyal adenokarsinom hücrelerinin reçineye karşı en yüksek hassasiyete sahip olduğunu göstermiştir. Bu çalışma, reçinenin farklı kanser hücrelerinin büyümesini engellediğine dair ilk kanıtları sunmaktadır.
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Birincil Dil en
Konular Biyoloji
Bölüm ARAŞTIRMA MAKALESİ (Research Article)

Orcid: 0000-0001-6327-4779
Yazar: Esra UÇAR SÖZMEN (Sorumlu Yazar)
Ülke: Andorra

Orcid: 0000-0003-1057-2558
Ülke: Turkey

Orcid: 0000-0003-4503-3036
Yazar: Mustafa ULU
Ülke: Turkey

Orcid: 0000-0001-8394-2746
Yazar: Aşkın AKPOLAT
Ülke: Turkey

Teşekkür The authors would like to acknowledge the contribution of the late Hanife Öğütlü for plant resin support in the realization of these studies. We hope that she rests in peace. Cytotoxic activity studies were conducted at Sivas Cumhuriyet University Advanced Technology Application and Research Center (CUTAM).

Başvuru Tarihi : 16 Nisan 2020
Kabul Tarihi : 31 Mayıs 2020
Yayımlanma Tarihi : 31 Aralık 2020

APA Uçar Sözmen, E , Şahi̇n Bölükbaşı, S , Ulu, M , Akpolat, A . (2020). The Determination of In vitro Antioxidant and Cytotoxic Activities of Resin Obtained from Cilician Fir (Abies cilicica (Antoine & Kotschy) Carrière) . Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 23 (6) , 1503-1509 . DOI: 10.18016/ksutarimdoga.vi.720675