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Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.)

Yıl 2022, Cilt: 25 Sayı: 6, 1234 - 1242, 30.12.2022

Muhammed Akif Açıkgöz , Ebru Batı Ay , Ahmet Aygün , Şevket Metin Kara

https://doi.org/10.18016/ksutarimdoga.vi.926160
Cited By: 1

Öz

Turkish yarrow (Achillea gypsicola Hub. Mor.) contains medicinally valuable bioactive metabolites. Light plays a key role in many physiological events in plants such as the accumulation of phenylpropanoid metabolites, particularly photosynthesis. The aim of this study was to determine the effects of light on the biosynthesis of phenylpropanoid metabolites and biomass growth in Turkish yarrow cell suspension cultures. In vitro plantlets obtained from seeds were used as explant sources and cell cultures of A. gypsicola were established using stem segment-derived callus tissues. Then, 8-day-old cell cultures kept in a B5 medium supplemented with 0.5 mg/L BA (benzylaminopurine)+0.5 mg/L NAA (naphthalene acetic acid) were incubated in light and dark environments. Cells were harvested five times (1st, 2nd, 3rd, 6th, and 12th day) in total. The amount of camphor was determined by gas chromatography-mass spectrometry (GC–MS). Other parameters were calculated using the spectrophotometric method. The total phenolic amount increased depending on the sampling time in dark and light conditions. Phenolic accumulation was increased by 29.25% compared to the initial culture. Also, light application increased the amount of camphor 1.12 times, the amount of flavanol 4.9 times, and the amount of flavonol 0.5 times. These results clearly demonstrate the effect of light on cell growth and phenylpropanoid metabolite accumulation in A. gypsicola cell cultures.

Anahtar Kelimeler

Abiotic elicitor Bioactive compounds Callus culture Secondary metabolites

Destekleyen Kurum

TÜBİTAK

Proje Numarası

1001- 114O564.

Teşekkür

The authors are grateful to the Scientific and Technological Research Council of Turkey (TÜBİTAK) for providing financial support for this work under Project No. 1001- 114O564. This study is a part of Dr Muhammed Akif Açıkgöz’s doctorate thesis.

Kaynakça

  • Abbasi BH, Stiles AR, Saxena PK, Liu CZ 2012. Gibberellic acid increases secondary metabolite production in Echinacea purpurea hairy roots. Applied Biochemistry and Biotechnology, 168(7): 2057–2066.
  • Abdel-Rahman R, Alqasoumi S, El-Desoky A, Soliman G, Paré G, Hegazy ME 2015. Evaluation of the anti-inflammatory, analgesic and anti-ulcerogenic potentials of Achillea fragrantissima (Forssk.). South African Journal of Botany, 98: 122-127.
  • Açıkgöz MA 2017. Achillea gypsicola türünde kallus kültürü ile sekonder metabolit üretim potansiyelinin belirlenmesi. Ordu Üniversitesi, Doktora Tezi (Basılı), Ordu.
  • Açıkgöz MA, Kara ŞM, Batı E, Odabaş S 2018. Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures. Akademik Ziraat Dergisi, 7(2): 179-184.
  • Açıkgöz MA, Kara ŞM, Aygün A, Özcan MM, Ay EB 2019. Effects of methyl jasmonate and salicylic acid on the production of camphor and phenolic compounds in cell suspension culture of endemic Turkish yarrow (Achillea gypsicola) species. Turkish Journal of Agriculture and Forestry, 43(3): 351-359.
  • Açıkgöz MA 2020. Establishment of cell suspension cultures of Ocimum basilicum L. and enhanced production of pharmaceutical active ingredients. Industrial Crops & Products. 148: 112278.
  • Açıkgöz MA 2021. Effects of sorbitol on the production of phenolic compounds and terpenoids in the cell suspension cultures of Ocimum basilicum L. Biologia, 76(1): 395-409.
  • Ahmad N, Rab A, Ahmad N 2016. Light induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert). Journal of Photochemistry and Photobiology B: Biology, 154: 51-56.
  • Ali M, Abbasi BH 2014. Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B Biology, 140: 223-227.
  • Anjum S, Abbasi BH, Doussot J, Favre-Réguillon A, Hano C 2017. Effects of photoperiod regimes and ultraviolet-C radiations on biosynthesis of industrially important lignans and neolignans in cell cultures of Linum usitatissimum L.(Flax). Journal of Photochemistry and Photobiology B: Biology, 167: 216-227.
  • Chan KY, Dorahy C, Tyler S 2007. Determining the agronomic value of composts produced from greenwaste from metropolitan areas of New South Wales, Australia. Australian Journal of Experimental Agriculture 47: 1377-1382.
  • Cheng LH, Eugene ICW, Yu CS 2009. Essential oil compositions and bioactivities of the various parts of Cinnamomum camphora Sieb.var, Linaloolifera fujuta, Journal of Forestry Research, 2: 77-96.
  • Dağlioğlu Y, Açikgöz MA, Özcan MM, Kara ŞM 2022. Impact of application of alumina oxide nanoparticles on callus induction, pigment content, cell damage and antioxidant enzyme activities in Ocimum basilicum. Journal of International Environmental Application and Science, 17(1): 22-33.
  • Batı Ay E, Kocaman B, Karaca Öner E, Açıkgöz MA 2022. Effects of elicitors on secondary metabolite (SM) production and antioxidant activity in sweet basil (Ocimum basilicum L.) cell suspension cultures. Notulae Scientia Biologicae, 14(2): 11246-11246.
  • Edris AE 2007. Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents. Phytotherapy Research, 4: 308-323.
  • Fazal H, Abbasi BH, Ahmad N, Ali M, Ali S 2016. Sucrose induced osmotic stress and photoperiod regimes enhanced the biomass and production of antioxidant secondary metabolites in shake flask suspension cultures of Prunella vulgaris L. Plant Cell, Tissue and Organ Culture, 124(3): 573-581.
  • Georgieva L, Ivanov I, Marchev A, Aneva I, Denev P, Georgiev V, Pavlov A 2015. Protopine production by Fumaria cell suspension cultures: effect of light. Applied biochemistry and biotechnology, 176(1): 287-300.
  • Giusti MM, Wrolstad RE 2001. Characterization and measurement of anthocyanins by uv-visible spectroscopy. Current Protocols in Food Analytical Chemistry, John Wiley and Sons, Inc., Hoboken.
  • Kami C, Lorrain S, Hornitschek P, Fankhauser C 2010. Chapter two light regulated plant growth and development. Current Topics in Developmental Biology, 91: 29-66.
  • Khan MA, Abbasi BH, Ahmed N, Ali H 2013. Effects of light regimes on in vitro seed germination and silymarin content in Silybum marianum. Industrial Crops and Products, 46: 105-110.
  • Kumaran A, Joel Karunakaran R 2007. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT- Food Science and Technology, 40: 344–352.
  • Lin TC, Chu FH, Tseng YH, Tsai JB, Chang ST, Wang SY 2007. Bioactivity Investigation of Lauraceae trees grown in Taiwan. Pharmaceutical Biology, 8: 638-644.
  • Liu B, Zuo Z, Liu H, Liu X, Lin C 2011. Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light. Genes Development, 25: 1029–1034.
  • Moscatiello R, Baldan B, Navazio L 2013. Plant cell suspension cultures. Humana Press, Totowa. Merzlyak MN, Chivkunova OB 2000. Light stress induced pigment changes and evidence for anthocyanin photoprotection in apple fruit. Journal of Photochemistry and Photobiology B Biology, 55: 154–162.
  • Murthy HN, Dandin VS, Zhong JJ, Paek KY 2014. Strategies for enhanced production of plant secondary metabolites from cell and organ cultures. In Production of biomass and bioactive compounds using bioreactor technology. Springer Netherlands, 471-508.
  • Nieto-Trujillo A, Buendía-González L, García-Morales C, Román-Guerrero A, Cruz-Sosa F, Estrada-Zúñiga ME 2017. Phenolic compounds and parthenolıde productıon from in vitro cultures of Tanacetum parthenium. Revista Mexicana de Ingeniería Química, 16(2): 371-383.
  • Saeidnia S, Ghohari AR, Mokhber-Dezfuli N, Kiuchi F 2011. A review on phytochemistry and medicinal properties of the genus Achillea. Daru. 19( 3): 173– 186.
  • Schwinn KE, Davies KM 2004. Flavonoids, in plant pigments and their manipulation. Annual Plant Reviews, Vol 14 (Ed. K Davies), Blackwell Publishing, 92-149.
  • Shohael AM, Ali MB, Yu KW, Hahn EJ, Islam R, Paek KY 2006. Effect of light on oxidative stress, secondary metabolites, and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochemistry, 41(5): 1179-1185.
  • Silva A, Cavero S, Begley V, Solé C, Böttcher R, Chávez S, de Nadal E 2017. Regulation of transcription elongation in response to osmostress. PLoS Genetics, 13(11): e1007090.
  • Sreelakshmi Y, Sharma R 2008. Differential regulation of phenylalanine ammonia lyase activity and protein level by light in tomato seedlings. Plant Physiology and Biochemistry, 46(4): 444-451.
  • Sun Y, Jan LY, Jan YN 1998. Transcriptional regulation of atonal during development of the Drosophila peripheral nervous system. Development 125(18): 3731--3740.
  • Tariq U, Ali M, Abbasi BH 2014. Morphogenic and biochemical variations under different spectral lights in callus cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B Biology, 130: 264-271.
  • Trojak M, Skowron E 2017. Improvement of plant heat tolerance by modification of xanthophyll cycle activity. World Scientific News, 70(2), 51-70.
  • Weidler G, Zur Oven-Krockhaus, S, Heunemann M, Orth C, Schleifenbaum F, Harter K, Hoecker U, Batschauer A 2012 Degradation of Arabidopsis CRY2 is regulated by SPA proteins and phytochrome A. Plant Cell, 24: 2610–2623.
  • Zhao SZ, Sun HZ, Chen M, Wang BS 2010. Light-regulated betacyanin accumulation in euhalophyte Suaeda salsa calli. Plant Cell, Tissue and Organ Culture (PCTOC), 102(1): 99-107.
  • Zuccarini P 2009. Camphor: Risks and benefits of a widely used natural product. Journal Applied Science Environmental Management, 2: 69-74.
  • Zuo Z, Liu H, Liu B, Liu X, Lin C 2011. Blue light-dependent interaction of CRY2 with SPA1 regulates COP1 activity and floral initiation in Arabidopsis. Current Biology, 21(10): 841-847.

Türk Civanperçemi (Achillea gypsicola Hub. Mor.) Hücre Süspansiyon Kültürlerinde Fenilpropanoid Metabolitlerin Işık Aracılığıyla Biosentezi

Yıl 2022, Cilt: 25 Sayı: 6, 1234 - 1242, 30.12.2022

Muhammed Akif Açıkgöz , Ebru Batı Ay , Ahmet Aygün , Şevket Metin Kara

https://doi.org/10.18016/ksutarimdoga.vi.926160
Cited By: 1

Öz

Türk civanperçemi (Achillea gypsicola Hub. Mor.) tıbbi açıdan değerli metabolitler içerir. Işık, bitkilerde başta fotosentez olmak üzere fenipropanoid metabolitlerin birikimi gibi birçok fizyolojik olayda kilit bir rol oynar. Bu çalışmanın amacı, ışığın Türk civanperçemi hücre süspansiyon kültüründe fenilpropanoid metabolitlerin biyosentezi ve biyokütle artışı üzerindeki etkilerini belirlemektir. Tohumlardan elde edilen in vitro bitkicikler eksplant kaynağı olarak kullanılmış ve gövde segmentinden türetilen kallus dokularından hücre kültürleri oluşturulmuştur. Daha sonra, 0.5 mg/L BA (benzilaminopurin)+0.5 mg/L NAA (naftalen asetik asit) ile desteklenmiş B5 ortamında muhafaza edien 8 günlük hücre kültürleri, aydınlık ve karanlık ortamlarda inkübe edilmiştir. Toplamda beş kez (1, 2, 3, 6 ve 12. gün) hücre hasadı yapılmıştır. Kamfor miktarı gas chromatography-mass spectrometry (GC-MS) ile belirlenmiştir. Diğer parametreler spektrofotometrik yöntem kullanılarak hesaplanmıştır. Toplam fenolik miktarı karanlık ve aydınlık koşullarda örnek alma zamanına bağlı olarak artış göstermiştir. Fenolik madde birikimi başlangıç kültürüne göre %29.25 artmıştır. Işık uygulaması kamfor miktarını 1.12 kat, flavanol miktarını 4.9 kat ve flavonol miktarını 0.5 kat artırmıştır. Bu sonuçlar bize A. gypsicola hücre kültürlerinde ışığın hücre büyümesi ve sekonder metabolit birikimindeki etkisini açıkça ortaya koymuştur.

Anahtar Kelimeler

Abiyotik elisitör Biyoaktif bileşikler Kallus kültürü Sekonder metabolit

Proje Numarası

1001- 114O564.

Kaynakça

  • Abbasi BH, Stiles AR, Saxena PK, Liu CZ 2012. Gibberellic acid increases secondary metabolite production in Echinacea purpurea hairy roots. Applied Biochemistry and Biotechnology, 168(7): 2057–2066.
  • Abdel-Rahman R, Alqasoumi S, El-Desoky A, Soliman G, Paré G, Hegazy ME 2015. Evaluation of the anti-inflammatory, analgesic and anti-ulcerogenic potentials of Achillea fragrantissima (Forssk.). South African Journal of Botany, 98: 122-127.
  • Açıkgöz MA 2017. Achillea gypsicola türünde kallus kültürü ile sekonder metabolit üretim potansiyelinin belirlenmesi. Ordu Üniversitesi, Doktora Tezi (Basılı), Ordu.
  • Açıkgöz MA, Kara ŞM, Batı E, Odabaş S 2018. Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures. Akademik Ziraat Dergisi, 7(2): 179-184.
  • Açıkgöz MA, Kara ŞM, Aygün A, Özcan MM, Ay EB 2019. Effects of methyl jasmonate and salicylic acid on the production of camphor and phenolic compounds in cell suspension culture of endemic Turkish yarrow (Achillea gypsicola) species. Turkish Journal of Agriculture and Forestry, 43(3): 351-359.
  • Açıkgöz MA 2020. Establishment of cell suspension cultures of Ocimum basilicum L. and enhanced production of pharmaceutical active ingredients. Industrial Crops & Products. 148: 112278.
  • Açıkgöz MA 2021. Effects of sorbitol on the production of phenolic compounds and terpenoids in the cell suspension cultures of Ocimum basilicum L. Biologia, 76(1): 395-409.
  • Ahmad N, Rab A, Ahmad N 2016. Light induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert). Journal of Photochemistry and Photobiology B: Biology, 154: 51-56.
  • Ali M, Abbasi BH 2014. Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B Biology, 140: 223-227.
  • Anjum S, Abbasi BH, Doussot J, Favre-Réguillon A, Hano C 2017. Effects of photoperiod regimes and ultraviolet-C radiations on biosynthesis of industrially important lignans and neolignans in cell cultures of Linum usitatissimum L.(Flax). Journal of Photochemistry and Photobiology B: Biology, 167: 216-227.
  • Chan KY, Dorahy C, Tyler S 2007. Determining the agronomic value of composts produced from greenwaste from metropolitan areas of New South Wales, Australia. Australian Journal of Experimental Agriculture 47: 1377-1382.
  • Cheng LH, Eugene ICW, Yu CS 2009. Essential oil compositions and bioactivities of the various parts of Cinnamomum camphora Sieb.var, Linaloolifera fujuta, Journal of Forestry Research, 2: 77-96.
  • Dağlioğlu Y, Açikgöz MA, Özcan MM, Kara ŞM 2022. Impact of application of alumina oxide nanoparticles on callus induction, pigment content, cell damage and antioxidant enzyme activities in Ocimum basilicum. Journal of International Environmental Application and Science, 17(1): 22-33.
  • Batı Ay E, Kocaman B, Karaca Öner E, Açıkgöz MA 2022. Effects of elicitors on secondary metabolite (SM) production and antioxidant activity in sweet basil (Ocimum basilicum L.) cell suspension cultures. Notulae Scientia Biologicae, 14(2): 11246-11246.
  • Edris AE 2007. Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents. Phytotherapy Research, 4: 308-323.
  • Fazal H, Abbasi BH, Ahmad N, Ali M, Ali S 2016. Sucrose induced osmotic stress and photoperiod regimes enhanced the biomass and production of antioxidant secondary metabolites in shake flask suspension cultures of Prunella vulgaris L. Plant Cell, Tissue and Organ Culture, 124(3): 573-581.
  • Georgieva L, Ivanov I, Marchev A, Aneva I, Denev P, Georgiev V, Pavlov A 2015. Protopine production by Fumaria cell suspension cultures: effect of light. Applied biochemistry and biotechnology, 176(1): 287-300.
  • Giusti MM, Wrolstad RE 2001. Characterization and measurement of anthocyanins by uv-visible spectroscopy. Current Protocols in Food Analytical Chemistry, John Wiley and Sons, Inc., Hoboken.
  • Kami C, Lorrain S, Hornitschek P, Fankhauser C 2010. Chapter two light regulated plant growth and development. Current Topics in Developmental Biology, 91: 29-66.
  • Khan MA, Abbasi BH, Ahmed N, Ali H 2013. Effects of light regimes on in vitro seed germination and silymarin content in Silybum marianum. Industrial Crops and Products, 46: 105-110.
  • Kumaran A, Joel Karunakaran R 2007. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT- Food Science and Technology, 40: 344–352.
  • Lin TC, Chu FH, Tseng YH, Tsai JB, Chang ST, Wang SY 2007. Bioactivity Investigation of Lauraceae trees grown in Taiwan. Pharmaceutical Biology, 8: 638-644.
  • Liu B, Zuo Z, Liu H, Liu X, Lin C 2011. Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light. Genes Development, 25: 1029–1034.
  • Moscatiello R, Baldan B, Navazio L 2013. Plant cell suspension cultures. Humana Press, Totowa. Merzlyak MN, Chivkunova OB 2000. Light stress induced pigment changes and evidence for anthocyanin photoprotection in apple fruit. Journal of Photochemistry and Photobiology B Biology, 55: 154–162.
  • Murthy HN, Dandin VS, Zhong JJ, Paek KY 2014. Strategies for enhanced production of plant secondary metabolites from cell and organ cultures. In Production of biomass and bioactive compounds using bioreactor technology. Springer Netherlands, 471-508.
  • Nieto-Trujillo A, Buendía-González L, García-Morales C, Román-Guerrero A, Cruz-Sosa F, Estrada-Zúñiga ME 2017. Phenolic compounds and parthenolıde productıon from in vitro cultures of Tanacetum parthenium. Revista Mexicana de Ingeniería Química, 16(2): 371-383.
  • Saeidnia S, Ghohari AR, Mokhber-Dezfuli N, Kiuchi F 2011. A review on phytochemistry and medicinal properties of the genus Achillea. Daru. 19( 3): 173– 186.
  • Schwinn KE, Davies KM 2004. Flavonoids, in plant pigments and their manipulation. Annual Plant Reviews, Vol 14 (Ed. K Davies), Blackwell Publishing, 92-149.
  • Shohael AM, Ali MB, Yu KW, Hahn EJ, Islam R, Paek KY 2006. Effect of light on oxidative stress, secondary metabolites, and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochemistry, 41(5): 1179-1185.
  • Silva A, Cavero S, Begley V, Solé C, Böttcher R, Chávez S, de Nadal E 2017. Regulation of transcription elongation in response to osmostress. PLoS Genetics, 13(11): e1007090.
  • Sreelakshmi Y, Sharma R 2008. Differential regulation of phenylalanine ammonia lyase activity and protein level by light in tomato seedlings. Plant Physiology and Biochemistry, 46(4): 444-451.
  • Sun Y, Jan LY, Jan YN 1998. Transcriptional regulation of atonal during development of the Drosophila peripheral nervous system. Development 125(18): 3731--3740.
  • Tariq U, Ali M, Abbasi BH 2014. Morphogenic and biochemical variations under different spectral lights in callus cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B Biology, 130: 264-271.
  • Trojak M, Skowron E 2017. Improvement of plant heat tolerance by modification of xanthophyll cycle activity. World Scientific News, 70(2), 51-70.
  • Weidler G, Zur Oven-Krockhaus, S, Heunemann M, Orth C, Schleifenbaum F, Harter K, Hoecker U, Batschauer A 2012 Degradation of Arabidopsis CRY2 is regulated by SPA proteins and phytochrome A. Plant Cell, 24: 2610–2623.
  • Zhao SZ, Sun HZ, Chen M, Wang BS 2010. Light-regulated betacyanin accumulation in euhalophyte Suaeda salsa calli. Plant Cell, Tissue and Organ Culture (PCTOC), 102(1): 99-107.
  • Zuccarini P 2009. Camphor: Risks and benefits of a widely used natural product. Journal Applied Science Environmental Management, 2: 69-74.
  • Zuo Z, Liu H, Liu B, Liu X, Lin C 2011. Blue light-dependent interaction of CRY2 with SPA1 regulates COP1 activity and floral initiation in Arabidopsis. Current Biology, 21(10): 841-847.
Toplam 38 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Muhammed Akif Açıkgöz 0000-0003-2436-5605

Ebru Batı Ay 0000-0002-9210-6907

Ahmet Aygün 0000-0001-7745-3380

Şevket Metin Kara 0000-0001-7755-1394

Proje Numarası 1001- 114O564.
Yayımlanma Tarihi 30 Aralık 2022
Gönderilme Tarihi 22 Nisan 2021
Kabul Tarihi 7 Aralık 2021
Yayımlandığı Sayı Yıl 2022Cilt: 25 Sayı: 6

Kaynak Göster

APA Açıkgöz, M. A., Batı Ay, E., Aygün, A., Kara, Ş. M. (2022). Light-Mediated Biosynthesis of Phenylpropanoid Metabolites in Cell Suspension Cultures of Turkish Yarrow (Achillea gypsicola Hub. Mor.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(6), 1234-1242. https://doi.org/10.18016/ksutarimdoga.vi.926160

Cited By

Influence of Selected Heavy Metals on Cell Growth and Camphor Secretion in Achillea gypsicola Hub. Mor. In Vitro Cell Cultures
Journal of Agricultural Faculty of Gaziosmanpasa University
https://doi.org/10.55507/gopzfd.1185552
 Kapak Resmi İndir

21082



2022-JIF = 0.500

2022-JCI = 0.170

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