Evaluation of Agronomic Traits and Allele Specific DNA Markers Related to Some Disease and Quality Traits in Mutant Karakılçık M4 Individuals

İlker Yüce; Ziya Dumlupınar

doi:10.18016/ksutarimdoga.vi.1120224

Araştırma Makalesi

Evaluation of Agronomic Traits and Allele Specific DNA Markers Related to Some Disease and Quality Traits in Mutant Karakılçık M4 Individuals

Yıl 2023, Cilt: 26 Sayı: 4, 861 - 869, 31.08.2023

İlker Yüce , Ziya Dumlupınar

https://doi.org/10.18016/ksutarimdoga.vi.1120224

Cited By: 3

Öz

Karakılçık (KK) is a landrace, of which both bread and durum wheat forms exist. This is the first study that reports mutation induction and detection with agronomic traits and allele specific markers on KK durum wheat landrace. In the study, KK landrace was induced with chemical mutation using sodium azide (NaN3) (3 mM) mutagen to improve agronomic traits. In the research, KK genotype and the 13 M4 individuals (KK-1 to KK-13) were used as plant materials. According to the results, the shortest mutant genotype was KK-10 (125.80 cm), with the highest protein ratio (18.50%) and wet gluten ratio (37.10%), while KK-9 genotype had the highest grain yield (4285.6 kg ha-1). The average polymorphism information content (PIC) was calculated as 0.97, while the average allele number was 15.2 per marker. Glu-B1 (Bx7OE primer) allele was determined on KK-11 and KK-13 genotypes. Wx-A1 allele was found on KK-1, KK-2, KK-4, KK-5 and KK-7 genotypes. Sr49 allele (Sun209) was determined on KK-5 genotype, while Yr45 allele (Xwgp118) was detected on KK-10 genotype. In addition, the high protein content allele Gpc-B1 (UHW89 primer) was found on KK-1, KK-2, KK-3 and KK-4 genotypes. Based on the principal component biplot analysis, it was determined that there was a positive relationship between grain yield (GY) with test weight (TW), grain number and weight per spike (GNS and GWS), and negative relationship with the other traits.

Anahtar Kelimeler

Karakılçık, Landrace, Agronomic traits, Principal components, Chemical mutation

Kaynakça

Al-Ashkar, I., Alderfasi, A., El-Hendawy, S., Al-Suhaibani, N., El-Kafafi, S. & Seleiman, M.F. (2019). Detecting Salt Tolerance in Doubled Haploid Wheat Lines. Agronomy, 9, 211. Doi: 10.3390/agronomy9040211 Alemu, Y.A., Anley, A.M. & Abebe, T.D. (2020). Genetic Variability and Association of Traits in Ethiopian Durum Wheat (Triticum turgidium L. var. durum) Landraces at Dabat Research Station, North Gondar. Cogent Food & Agriculture, 6 (1), 1778604. Doi: 10.1080/23311932.2020.1778604
Anonymous, (2021). Turkish State Meteorology Service. https://www.mgm.gov.tr/ ?il=Kahraman maras. Accessed on 15.09.2021
Aslan, D., Aktaş, H., Ordu, B. & Zencirci, N. (2017). Evaluation of Bread and Einkorn Wheat under in vitro Drought Stress. The Journal of Animal and Plant Sciences, 27 (6),1974-1983.
Aydemir, G., Dumlupinar, Z., Yüce, I., Baskonus, T., Sunulu, S. & Güngör, H. (2020). Evaluation of F5 Individuals Obtained from B28 × Kunduru-1149 Reciprocal Cross Population by Functional Markers. KSU Journal of Agriculture and Nature, 23 (4), 1005-1011. Doi: 10.18016/ksutarimdoga.vi. 687935
Bai, J., Yan, W., Wang, Y., Yin, Q., Liu, J., Wight, C. & Ma, B. (2018). Screening Oat Genotypes for Tolerance to Salinity and Alkalinity. Frontiers in Plant Science, 9, 1302. Doi: 10.3389/fpls.2018.01302
Bansal, U.K., Muhammad, S., Forrest, K.L., Hayden, M.J. & Bariana, H.S. (2015). Mapping of A New Stem Rust Resistance Gene Sr49 in Chromosome 5B of Wheat. Theoretical and Applied Genetics, 128 (10), 2113-2119. Doi: 10.1007/s00122-015-2571-4
Butow, B.J., Ma, W., Gale, K.R., Cornish, G.B., Rampling, L., Larroque, O., Morell, M.K. & Bekes, F. (2003). Molecular Discrimination of Bx7OE Alleles Demonstrates That A Highly Expressed High-Molecular-Weight Glutenin Allele Has A Major Impact on Wheat Flour Dough Strength. Theoretical and Applied Genetics, 107 (8), 1524‒1532. Doi: 10.1007/s00122-003-1396-8
Dice, L.R. (1945). Measures of The Amount of Ecologic Association Between Species. Ecology, 26, 297- 302.
Distelfeld, A., Uauy, C., Fahimaand, T. & Dubcovsky, J. (2006). Physical Map of The Wheat High-Grain Protein Content Gene Gpc-B1 and Development of A High Throughput Molecular Marker. New Phytologist, 169, 753‒763. Doi:10.1111/j.1469-8137.2005.01627.x
Durland, J. & Ahmadian-Moghadam, H. (2022). Genetics, Mutagenesis. [Updated 2021 Sep 21]. In: StatPearls [Internet]. Treasure Island (FL), StatPearls Publishing; 2022 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK560519/
FAO, (2020). FAO Statistical Databases. http:// www.fao.org/faostat/en/#data. Accessed 15.06.2021
Fu, B.X., Wang, K., Dupuis, B., Taylor, D. & Nam, S. (2018). Kernel Vitreousness and Protein Content: Relationship, Interaction and Synergistic Effects on Durum Wheat Quality. Journal of Cereal Science, 79, 210-217. Doi: 10.1016/j.jcs.2017.09.003
Güngör, H., Çikili, Y. & Dumlupinar, Z. (2019). Evaluation of Morpho-Physiological Traits of Turkish Rice Genotypes in Response to Salt Stress under in vitro Conditions. Journal of Animal and Plant Sciences, 29, 556-567.
Güngör, H. & Dumlupınar, Z. (2019). Evaluation of Some Bread Wheat (Triticum aestivum L.) Cultivars for Yield, Yield Components and Quality Traits in Bolu Conditions. Turkish Journal of Agricultural and Natural Science, 6 (1), 44-51. Doi: 10.30910/turkjans.515346
Howarth, C.J., Martinez-Martin, P.M.J., Cowan, A.A., Griffiths, I.M., Sanderson, R., Lister, S.J., Langdon, T., Clarke, S., Fradgley, N. & Marshall, A.H. (2021). Genotype and Environment Affect The Grain Quality and Yield of Winter Oats (Avena sativa L.). Foods, 10, 2356. Doi: 10.3390/foods10102356
Kiraz, H., Yüce, İ., Kekilli, Ö., Ocaktan, H., Topsakal, M., Gürocak, N.Y., Osanmaz, H., Kılınç, F.M., Başkonuş, T. & Dumlupınar, Z. (2019). Characterization of M3 Mutants of Seri 82 Bread Wheat Cultivar Using Functional Markers. Black Sea Journal of Agriculture, 2 (4), 194-202.
Koçyiğit, B.K., Yüce, İ., Başkonuş, T., Dokuyucu, T., Akkaya, A. & Dumlupinar, Z. (2021). Evaluation of F4 Individuals Belong to Seri 82 × B35 Bread Wheat (Triticum aestivum L.) Cross Population Using Functional DNA Markers. KSU Journal of Agriculture and Nature, 24 (3), 586-593. Doi: 10.18016/ksutarimdoga.vi.752972
Li, Q., Chen, X.M., Wang, M.N. & Jing, J.X. (2011). Yr45, A New Wheat Gene for Stripe Rust Resistance on The Long Arm of Chromosome 3D. Theoretical and Applied Genetics, 122 (1), 189-197. Doi: 10.1007/s00122-010-1435-1
Mehrabi, A.A., Pour-Aboughadareh, A., Mansouri, S. & Hosseini, A. (2020). Genome-wide Association Analysis of Root System Architecture Features and Agronomic Traits in Durum Wheat. Molecular Breeding, 40, 55. Doi: 10.1007/s11032-020-01136-6
Nehe, A., Akin, B., Sanal, T., Evlice, A.K., Unsal, R., Dincer, N., Demir, L., Geren, H., Sevim, I., Orhan, Ş., Yaktubay, S., Ezici, A., Guzman, C. & Morgounov, A. (2019). Genotype x Environment Interaction and Genetic Gain for Grain Yield and Grain Quality Traits in Turkish Spring Wheat Released between 1964 and 2010. PLoS ONE 14(7), e0219432. Doi: 10.1371/journal. pone.0219432
Oliver, R.E., Obert, D.E., Hu, G., Bonman, J.M., O’Leary-Jepsen, E. & Jackson, E.W. (2010). Development of Oat-Based Markers from Barley and Wheat Microsatellites. Genome, 53 (6), 458-471. Doi: 10.1139/G10-021
Philipp, N., Weichert, H., Bohra, U., Weschke, W., Schulthess, A.W. & Weber, H. (2018). Grain Number and Grain Yield Distribution Along The Spike Remain Stable Despite Breeding for High Yield in Winter Wheat. PLoS ONE, 13 (10),e0205452. Doi: 10.1371/journal.pone.0205452
Rohlf, F.J. (2005). NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.2.
Shah, L., Yahya, M., Shah, S.M.A., Nadeem, M., Ali, A., Ali, A., Wang, J., Riaz, M.W., Rehman, S., Wu, W., Khan, R.M., Abbas, A., Riaz, A., Anis, G.B., Si, H., Jiang, H. & Ma, C. (2019). Improving Lodging Resistance: Using Wheat and Rice as Classical Examples. International Journal of Molecular Sciences, 20(17), 4211. Doi: 10.3390/ijms20174211
Shariflou, M.R. & Sharp, P.J. (1999). A Polymorphic Microsatellite in The 3’end of ‘Waxy’ Genes of Wheat,Triticum aestivum. Plant Breeding, 118, 275 -277.Doi:10.1046/j.1439-0523.1999.118003275. x
Saal, B. & Wricke, G. 1999. Development of Simple Sequence Repeat Markers in Rye (Secale cereale L.). Genome, 42, 964–972. Doi: 10.1139/g99-052
Tsonev, S., Christov, N.K., Mihova, G., Dimitrova, A. & Georgieva Todorovska, E. (2021). Genetic Diversity and Population Structure of Bread Wheat Varieties Grown in Bulgaria Based on Microsatellite and Phenotypic Analyses. Biotechnology & Biotechnological Equipment, 35(1), 1520-1533. Doi: 10.1080/13102818.2021. 1996274
Vanzetti, L.S., Yerkovich, N., Chialvo, E., Lombardo, L., Vaschetto, L. & Helguera, M. (2013). Genetic Structure of Argentinean Hexaploid Wheat Germplasm. Genetics and Molecular Biology, 36, 391-399. Doi: 10.1590/S1415-47572013000300014
Yildirim, A. & Atasoy, A. (2020). Quality Characteristics of Some Durum Wheat Varieties Grown in Southeastern Anatolia Region of Turkey (GAP). Harran Journal of Agriculture and Food Science, 24(4), 420-431. Doi: 10.29050/harran ziraat.738505
Wang, Y., Wang, S., Jia, X., Tian, Z., Wang, Y., Wang, C., Zhang, H., Liu, X., Zhao, J., Deng, P. & Ji, W. (2021). Chromosome Karyotype and Stability of New Synthetic Hexaploid Wheat. Molecular Breeding,41, 1–12.Doi:10.1007/s11032-021-01253w
Weir, B.S. (1996). Genetic Data Analysis II. Sinauer Associates Inc., Sunderland, England.
Wolde, G.M., Mascher, M. & Schnurbusch, T. (2019). Genetic Modification of Spikelet Arrangement in Wheat Increases Grain Number without Significantly Affecting Grain Weight. Molecular Genetics and Genomics, 294 (2), 457-468. Doi: 10.1007/s00438-018-1523-5
Woźniak, A. (2020). Effect of Cereal Monoculture and Tillage Systems on Grain Yield and Weed Infestation of Winter Durum Wheat. International Journal of Plant Production, 14 (1), 1-8. Doi: 10.1007/s42106-019-00062-8

Mutant Karakılçık M4 Bireylerinde Agronomik Özellikler, Bazı Hastalık ve Kalite Özellikleri ile İlişkili Allellerin DNA Markörleri ile Saptanması

Yıl 2023, Cilt: 26 Sayı: 4, 861 - 869, 31.08.2023

İlker Yüce , Ziya Dumlupınar

https://doi.org/10.18016/ksutarimdoga.vi.1120224

Cited By: 3

Öz

Karakılçık (KK), hem ekmeklik hem de makarnalık buğday formları bulunan yerel bir çeşittir. Bu, yerel KK makarnalık buğday çeşidinde agronomik özellikler ve allel spesifik markörler ile mutasyon tespitini bildiren ilk çalışmadır. Çalışmada, agronomik özellikleri iyileştirmek için sodyum azid (NaN3) (3 mM) mutajeni kullanılarak kimyasal mutasyon ile yerel KK çeşidi muamele edilmiştir. Araştırmada bitki materyali olarak KK genotipi ve 13 M4 bireyi (KK-1 ila KK-13) kullanılmıştır. Elde edilen sonuçlara göre, en yüksek protein oranı (%18.50), yaş gluten oranı (%37.10) ile en kısa mutant genotip KK-10 (125.80 cm) olurken, KK-9 en yüksek tane verimine (4285.6 kg ha-1) sahip genotip olmuştur. Ortalama polimorfizm bilgi içeriği (PIC) 0.97, ortalama allel sayısı markör başına 15.2 olarak hesaplanmıştır. Glu-B1 (Bx7OE) alleli, KK-11 ve KK-13 genotiplerinde, Wx-A1 alleli ise KK-1, KK-2, KK-4, KK-5 ve KK-7 genotiplerinde belirlenmiştir. KK-5 genotipinde Sr49 alleli (Sun209), KK-10 genotipinde ise Yr45 alleli (Xwgp118) tespit edilmiştir. Ayrıca KK-1, KK-2, KK-3 ve KK-4 genotiplerinde yüksek protein içerikli allel olan Gpc-B1 alleli (UHW89) bulunmuştur. Temel bileşen biplot analizine göre, tane verimi (TV) ile hektolitre ağırlığı (HL), başakta tane sayısı ve başakta tane ağırlığı (BTS ve BTA) arasında pozitif, diğer özelliklerle negatif ilişki olduğu belirlenmiştir.

Anahtar Kelimeler

Karakılçık, Yerel çeşit, Tarımsal özellikler, Temel bileşenler, Kimyasal mutasyon

Kaynakça

Al-Ashkar, I., Alderfasi, A., El-Hendawy, S., Al-Suhaibani, N., El-Kafafi, S. & Seleiman, M.F. (2019). Detecting Salt Tolerance in Doubled Haploid Wheat Lines. Agronomy, 9, 211. Doi: 10.3390/agronomy9040211 Alemu, Y.A., Anley, A.M. & Abebe, T.D. (2020). Genetic Variability and Association of Traits in Ethiopian Durum Wheat (Triticum turgidium L. var. durum) Landraces at Dabat Research Station, North Gondar. Cogent Food & Agriculture, 6 (1), 1778604. Doi: 10.1080/23311932.2020.1778604
Anonymous, (2021). Turkish State Meteorology Service. https://www.mgm.gov.tr/ ?il=Kahraman maras. Accessed on 15.09.2021
Aslan, D., Aktaş, H., Ordu, B. & Zencirci, N. (2017). Evaluation of Bread and Einkorn Wheat under in vitro Drought Stress. The Journal of Animal and Plant Sciences, 27 (6),1974-1983.
Aydemir, G., Dumlupinar, Z., Yüce, I., Baskonus, T., Sunulu, S. & Güngör, H. (2020). Evaluation of F5 Individuals Obtained from B28 × Kunduru-1149 Reciprocal Cross Population by Functional Markers. KSU Journal of Agriculture and Nature, 23 (4), 1005-1011. Doi: 10.18016/ksutarimdoga.vi. 687935
Bai, J., Yan, W., Wang, Y., Yin, Q., Liu, J., Wight, C. & Ma, B. (2018). Screening Oat Genotypes for Tolerance to Salinity and Alkalinity. Frontiers in Plant Science, 9, 1302. Doi: 10.3389/fpls.2018.01302
Bansal, U.K., Muhammad, S., Forrest, K.L., Hayden, M.J. & Bariana, H.S. (2015). Mapping of A New Stem Rust Resistance Gene Sr49 in Chromosome 5B of Wheat. Theoretical and Applied Genetics, 128 (10), 2113-2119. Doi: 10.1007/s00122-015-2571-4
Butow, B.J., Ma, W., Gale, K.R., Cornish, G.B., Rampling, L., Larroque, O., Morell, M.K. & Bekes, F. (2003). Molecular Discrimination of Bx7OE Alleles Demonstrates That A Highly Expressed High-Molecular-Weight Glutenin Allele Has A Major Impact on Wheat Flour Dough Strength. Theoretical and Applied Genetics, 107 (8), 1524‒1532. Doi: 10.1007/s00122-003-1396-8
Dice, L.R. (1945). Measures of The Amount of Ecologic Association Between Species. Ecology, 26, 297- 302.
Distelfeld, A., Uauy, C., Fahimaand, T. & Dubcovsky, J. (2006). Physical Map of The Wheat High-Grain Protein Content Gene Gpc-B1 and Development of A High Throughput Molecular Marker. New Phytologist, 169, 753‒763. Doi:10.1111/j.1469-8137.2005.01627.x
Durland, J. & Ahmadian-Moghadam, H. (2022). Genetics, Mutagenesis. [Updated 2021 Sep 21]. In: StatPearls [Internet]. Treasure Island (FL), StatPearls Publishing; 2022 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK560519/
FAO, (2020). FAO Statistical Databases. http:// www.fao.org/faostat/en/#data. Accessed 15.06.2021
Fu, B.X., Wang, K., Dupuis, B., Taylor, D. & Nam, S. (2018). Kernel Vitreousness and Protein Content: Relationship, Interaction and Synergistic Effects on Durum Wheat Quality. Journal of Cereal Science, 79, 210-217. Doi: 10.1016/j.jcs.2017.09.003
Güngör, H., Çikili, Y. & Dumlupinar, Z. (2019). Evaluation of Morpho-Physiological Traits of Turkish Rice Genotypes in Response to Salt Stress under in vitro Conditions. Journal of Animal and Plant Sciences, 29, 556-567.
Güngör, H. & Dumlupınar, Z. (2019). Evaluation of Some Bread Wheat (Triticum aestivum L.) Cultivars for Yield, Yield Components and Quality Traits in Bolu Conditions. Turkish Journal of Agricultural and Natural Science, 6 (1), 44-51. Doi: 10.30910/turkjans.515346
Howarth, C.J., Martinez-Martin, P.M.J., Cowan, A.A., Griffiths, I.M., Sanderson, R., Lister, S.J., Langdon, T., Clarke, S., Fradgley, N. & Marshall, A.H. (2021). Genotype and Environment Affect The Grain Quality and Yield of Winter Oats (Avena sativa L.). Foods, 10, 2356. Doi: 10.3390/foods10102356
Kiraz, H., Yüce, İ., Kekilli, Ö., Ocaktan, H., Topsakal, M., Gürocak, N.Y., Osanmaz, H., Kılınç, F.M., Başkonuş, T. & Dumlupınar, Z. (2019). Characterization of M3 Mutants of Seri 82 Bread Wheat Cultivar Using Functional Markers. Black Sea Journal of Agriculture, 2 (4), 194-202.
Koçyiğit, B.K., Yüce, İ., Başkonuş, T., Dokuyucu, T., Akkaya, A. & Dumlupinar, Z. (2021). Evaluation of F4 Individuals Belong to Seri 82 × B35 Bread Wheat (Triticum aestivum L.) Cross Population Using Functional DNA Markers. KSU Journal of Agriculture and Nature, 24 (3), 586-593. Doi: 10.18016/ksutarimdoga.vi.752972
Li, Q., Chen, X.M., Wang, M.N. & Jing, J.X. (2011). Yr45, A New Wheat Gene for Stripe Rust Resistance on The Long Arm of Chromosome 3D. Theoretical and Applied Genetics, 122 (1), 189-197. Doi: 10.1007/s00122-010-1435-1
Mehrabi, A.A., Pour-Aboughadareh, A., Mansouri, S. & Hosseini, A. (2020). Genome-wide Association Analysis of Root System Architecture Features and Agronomic Traits in Durum Wheat. Molecular Breeding, 40, 55. Doi: 10.1007/s11032-020-01136-6
Nehe, A., Akin, B., Sanal, T., Evlice, A.K., Unsal, R., Dincer, N., Demir, L., Geren, H., Sevim, I., Orhan, Ş., Yaktubay, S., Ezici, A., Guzman, C. & Morgounov, A. (2019). Genotype x Environment Interaction and Genetic Gain for Grain Yield and Grain Quality Traits in Turkish Spring Wheat Released between 1964 and 2010. PLoS ONE 14(7), e0219432. Doi: 10.1371/journal. pone.0219432
Oliver, R.E., Obert, D.E., Hu, G., Bonman, J.M., O’Leary-Jepsen, E. & Jackson, E.W. (2010). Development of Oat-Based Markers from Barley and Wheat Microsatellites. Genome, 53 (6), 458-471. Doi: 10.1139/G10-021
Philipp, N., Weichert, H., Bohra, U., Weschke, W., Schulthess, A.W. & Weber, H. (2018). Grain Number and Grain Yield Distribution Along The Spike Remain Stable Despite Breeding for High Yield in Winter Wheat. PLoS ONE, 13 (10),e0205452. Doi: 10.1371/journal.pone.0205452
Rohlf, F.J. (2005). NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.2.
Shah, L., Yahya, M., Shah, S.M.A., Nadeem, M., Ali, A., Ali, A., Wang, J., Riaz, M.W., Rehman, S., Wu, W., Khan, R.M., Abbas, A., Riaz, A., Anis, G.B., Si, H., Jiang, H. & Ma, C. (2019). Improving Lodging Resistance: Using Wheat and Rice as Classical Examples. International Journal of Molecular Sciences, 20(17), 4211. Doi: 10.3390/ijms20174211
Shariflou, M.R. & Sharp, P.J. (1999). A Polymorphic Microsatellite in The 3’end of ‘Waxy’ Genes of Wheat,Triticum aestivum. Plant Breeding, 118, 275 -277.Doi:10.1046/j.1439-0523.1999.118003275. x
Saal, B. & Wricke, G. 1999. Development of Simple Sequence Repeat Markers in Rye (Secale cereale L.). Genome, 42, 964–972. Doi: 10.1139/g99-052
Tsonev, S., Christov, N.K., Mihova, G., Dimitrova, A. & Georgieva Todorovska, E. (2021). Genetic Diversity and Population Structure of Bread Wheat Varieties Grown in Bulgaria Based on Microsatellite and Phenotypic Analyses. Biotechnology & Biotechnological Equipment, 35(1), 1520-1533. Doi: 10.1080/13102818.2021. 1996274
Vanzetti, L.S., Yerkovich, N., Chialvo, E., Lombardo, L., Vaschetto, L. & Helguera, M. (2013). Genetic Structure of Argentinean Hexaploid Wheat Germplasm. Genetics and Molecular Biology, 36, 391-399. Doi: 10.1590/S1415-47572013000300014
Yildirim, A. & Atasoy, A. (2020). Quality Characteristics of Some Durum Wheat Varieties Grown in Southeastern Anatolia Region of Turkey (GAP). Harran Journal of Agriculture and Food Science, 24(4), 420-431. Doi: 10.29050/harran ziraat.738505
Wang, Y., Wang, S., Jia, X., Tian, Z., Wang, Y., Wang, C., Zhang, H., Liu, X., Zhao, J., Deng, P. & Ji, W. (2021). Chromosome Karyotype and Stability of New Synthetic Hexaploid Wheat. Molecular Breeding,41, 1–12.Doi:10.1007/s11032-021-01253w
Weir, B.S. (1996). Genetic Data Analysis II. Sinauer Associates Inc., Sunderland, England.
Wolde, G.M., Mascher, M. & Schnurbusch, T. (2019). Genetic Modification of Spikelet Arrangement in Wheat Increases Grain Number without Significantly Affecting Grain Weight. Molecular Genetics and Genomics, 294 (2), 457-468. Doi: 10.1007/s00438-018-1523-5
Woźniak, A. (2020). Effect of Cereal Monoculture and Tillage Systems on Grain Yield and Weed Infestation of Winter Durum Wheat. International Journal of Plant Production, 14 (1), 1-8. Doi: 10.1007/s42106-019-00062-8

Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Ziraat, Veterinerlik ve Gıda Bilimleri
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	İlker Yüce 0000-0002-9761-3561 Ziya Dumlupınar 0000-0003-3119-6926
Erken Görünüm Tarihi	15 Mayıs 2023
Yayımlanma Tarihi	31 Ağustos 2023
Gönderilme Tarihi	23 Mayıs 2022
Kabul Tarihi	27 Şubat 2023
Yayımlandığı Sayı	Yıl 2023Cilt: 26 Sayı: 4

Kaynak Göster

APA	Yüce, İ., & Dumlupınar, Z. (2023). Evaluation of Agronomic Traits and Allele Specific DNA Markers Related to Some Disease and Quality Traits in Mutant Karakılçık M4 Individuals. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(4), 861-869. https://doi.org/10.18016/ksutarimdoga.vi.1120224