EN
TR
Investigation of the Antidiabetic, Antitrosinase, Antioxidant, and Cytotoxic Effects of Different Extracts Prepared from Opuntia ficus-indica (L.) Mill. plant
Abstract
The Flora of Türkiye contains many plant species and varieties due to its different climate and ecological conditions. It has great economic potential in terms of cultivated and natural medicinal plants. In this study, the effects of extracts obtained from the fruit part of the O. ficus-indica plant by different extraction methods were examined on antidiabetic, antityrosinase, antioxidant, and cell viability. For this purpose, after drying the fruits, the extracts were prepared with %70 methanol by maceration and ultrasound-assisted extraction methods. Then the α-amylase, α-glucosidase, and tyrosinase inhibitory effects as well as antioxidant capacities with DPPH and ABTS methods and cell viability of extracts were determined on RL95-2 and A549 cancer cells. As a result, it was shown that, the inhibitory effect of the extract prepared by ultrasound-assisted extraction method on α-amylase (IC50=395.123±3.477 µg ml-1) and tyrosinase enzyme (IC50=551.633±1.159 µg ml-1 and ABTS radical scavenging activity (0.522±0.041 µMTrolox/gextract) was found to be higher than the other extract. It was also determined that the total phenolic and flavonoid content of the same extract was higher ((181.189±4,576 mgGAE/extract and 125.635±1.946 mgCA/extract). The effects of the extracts on α-glucosidase and tyrosinase enzymes and cell viability were found to be moderate. In conclusion, further studies are needed to understand which compounds were related to α-amylase inhibitory effect and antioxidant activity and mechanisms of action.
Keywords
Destekleyen Kurum
Erciyes Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi
Proje Numarası
Bu çalışma Erciyes Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi tarafından TLO-2023-12968 kodlu proje ile desteklenmiştir.
Kaynakça
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Ayrıntılar
Birincil Dil
Türkçe
Konular
Farmasotik Botanik
Bölüm
Araştırma Makalesi
Erken Görünüm Tarihi
15 Eylül 2024
Yayımlanma Tarihi
25 Aralık 2024
Gönderilme Tarihi
2 Nisan 2024
Kabul Tarihi
15 Ağustos 2024
Yayımlandığı Sayı
Yıl 1970 Cilt: 27 Sayı: Ek Sayı 1 (Suppl 1)
APA
Paşayeva, L., Kıcalı, S., & Karaboğa Arslan, A. K. (2024). Opuntia ficus-indica (L.) Mill. Bitkisinden Hazırlanan Farklı Ekstrelerin Antidiyabetik, Antitrozinaz, Antioksidan ve Sitotoksik Etkisinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1), 185-193. https://doi.org/10.18016/ksutarimdoga.vi.1463666
