Opuntia ficus-indica (L.) Mill. Bitkisinden Hazırlanan Farklı Ekstrelerin Antidiyabetik, Antitrozinaz, Antioksidan ve Sitotoksik Etkisinin Araştırılması

Leyla Paşayeva; Sena Kıcalı; Ayşe Kübra Karaboğa Arslan

doi:10.18016/ksutarimdoga.vi.1463666

EN TR

Investigation of the Antidiabetic, Antitrosinase, Antioxidant, and Cytotoxic Effects of Different Extracts Prepared from Opuntia ficus-indica (L.) Mill. plant

Abstract

The Flora of Türkiye contains many plant species and varieties due to its different climate and ecological conditions. It has great economic potential in terms of cultivated and natural medicinal plants. In this study, the effects of extracts obtained from the fruit part of the O. ficus-indica plant by different extraction methods were examined on antidiabetic, antityrosinase, antioxidant, and cell viability. For this purpose, after drying the fruits, the extracts were prepared with %70 methanol by maceration and ultrasound-assisted extraction methods. Then the α-amylase, α-glucosidase, and tyrosinase inhibitory effects as well as antioxidant capacities with DPPH and ABTS methods and cell viability of extracts were determined on RL95-2 and A549 cancer cells. As a result, it was shown that, the inhibitory effect of the extract prepared by ultrasound-assisted extraction method on α-amylase (IC50=395.123±3.477 µg ml-1) and tyrosinase enzyme (IC50=551.633±1.159 µg ml-1 and ABTS radical scavenging activity (0.522±0.041 µMTrolox/gextract) was found to be higher than the other extract. It was also determined that the total phenolic and flavonoid content of the same extract was higher ((181.189±4,576 mgGAE/extract and 125.635±1.946 mgCA/extract). The effects of the extracts on α-glucosidase and tyrosinase enzymes and cell viability were found to be moderate. In conclusion, further studies are needed to understand which compounds were related to α-amylase inhibitory effect and antioxidant activity and mechanisms of action.

Keywords

Opuntia ficus-indica (L.) Mill. Bitkisinden Hazırlanan Farklı Ekstrelerin Antidiyabetik, Antitrozinaz, Antioksidan ve Sitotoksik Etkisinin Araştırılması

Öz

Türkiye farklı iklim ve ekolojik koşullara sahip olması nedeniyle floranın çok sayıda bitki türü ve çeşidi içermesi bakımından doğadan toplanan ve kültürü yapılan tıbbi bitkiler açısından büyük bir ekonomik potansiyele sahiptir. Çalışmada Opuntia ficus-indica bitkisinin meyve kısmından farklı ekstraksiyon yöntemleriyle elde edilen ekstrelerin antidiyabetik, antitirozinaz, antioksidan ve hücre canlılığı üzerine etkileri incelenmiştir. Bu amaçla meyve kısmı kurutulduktan sonra %70’lik metanol ile maserasyon ve ses dalgaları-destekli sıvı ekstraksiyonu yöntemleriyle hazırlanan ekstrelerin α-amilaz, α-glikozidaz, tirozinaz inhibitör etkileri yanında antioksidan kapasiteleri DPPH ve ABTS yöntemleri ve hücre canlılığı üzerine etkileri ise RL95-2 ve A549 kanser hücrelerinde tayin edilmiştir. Sonuç olarak ekstrelerden ses dalgaları-destekli sıvı ekstraksiyonu yöntemiyle hazırlanan ekstrenin α-amilaz (IC50=395.123±3.477 µg ml-1) ve tirozinaz enzimi üzerinde inhibisyon etkisinin (IC50=551.633±1.159 µg ml-1), ABTS radikal süpürücü aktivitesinin (0.522±0.041 µMTrolox/gekstre) diğer ekstreden daha yüksek olduğu görülmüştür. Ayrıca aynı ekstresinin toplam fenolik medde ve flavonoit miktarının da daha yüksek olduğu belirlenmiştir ((181.189±4.576 mgGAE/gekstre ve 125.635±1.946 mgCA /gekstre ). Ekstrelerin α-glikozidaz, antitirozinaz ve hücre canlılığı üzerine etkileri orta düzeyde bulunmuştur. Bu çalışmanın sonuçları ile özellikle ses dalgaları-destekli sıvı ekstraksiyonu yöntemiyle hazırlanan ekstrenin α-amilaz inhibitör etki ve antioksidan etkisinin hangi bileşiklerden kaynaklandığını ve etki mekanizmalarını belirlemek için daha ileri çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler

Destekleyen Kurum

Erciyes Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi

Proje Numarası

Bu çalışma Erciyes Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi tarafından TLO-2023-12968 kodlu proje ile desteklenmiştir.

Kaynakça

Arıtuluk, Z. (2012). Halk arasında diyabete karşı kullanılan bitkiler Türkiye-II. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 32(2), 179-208.
Association, A. D. (2010). Diagnosis and classification of diabetes mellitus. Diabetes care, 33(Supplement_1), S62-S69. https://doi.org/ 10.2337/dc10-S062
Atiya, A., Majrashi, T. A., Begum, M. Y., Abdul Qadir, S. F., Alqahtani, A. S., Ali Alosman, A. S., Alahmari, A. A., Mesfer Al Aldabsh, A. N., Alshahrani, A. T., & Alshahrani, R. R. M. (2023). Influence of solvent selection and extraction methods on the determination of polyphenols, antioxidant, lipoxygenase and tyrosinase inhibition activities of Opuntia ficus-indica fruits peel and pulp collected from the Kingdom of Saudi Arabia (KSA). Nat Prod Res, 37(3), 514-521. https://doi.org/10.1080/ 14786419.2021.1983571
Belviranlı, B., Al‐Juhaimi, F., Özcan, M. M., Ghafoor, K., Babiker, E. E., & Alsawmahi, O. N. (2019). Effect of location on some physico‐chemical properties of prickly pear (Opuntia ficus‐indica L.) fruit and seeds. Journal of Food Processing and Preservation, 43(3), e13896.
Berraaouan, A., Abderrahim, Z., Hassane, M., Abdelkhaleq, L., Mohammed, A., & Mohamed, B. (2015). Evaluation of protective effect of cactus pear seed oil (Opuntia ficus-indica L. Mill.) against alloxan-induced diabetes in mice. Asian Pac J Trop Med, 8(7), 532-537. https://doi.org/10.1016/ j.apjtm.2015.06.013
Bourhia, M., Elmandaoui, H., Ullah, R., Ibenmoussa, S., & Shahat, A. A. (2020). Physicochemical evaluation of the fruit pulp of spp growing in the Mediterranean area under hard climate conditions. Open Chemistry, 18(1), 565-575. https://doi.org/ 10.1515/chem-2020-0097
Butterweck, V., Semlin, L., Feistel, B., Pischel, I., Bauer, K., & Verspohl, E. J. (2011). Comparative evaluation of two different Opuntia ficus-indica extracts for blood sugar lowering effects in rats. Phytother Res, 25(3), 370-375. https://doi.org/ 10.1002/ptr.3271
Cano, M. P., Gomez-Maqueo, A., Fernandez-Lopez, R., Welti-Chanes, J., & Garcia-Cayuela, T. (2019). Impact of high hydrostatic pressure and thermal treatment on the stability and bioaccessibility of carotenoid and carotenoid esters in astringent persimmon (Diospyros kaki Thunb, var. Rojo Brillante). Food Res Int, 123, 538-549. https://doi.org/10.1016/j.foodres.2019.05.017

Chavez-Santoscoy, R. A., Gutierrez-Uribe, J. A., & Serna-Saldivar, S. O. (2009). Phenolic composition, antioxidant capacity and in vitro cancer cell cytotoxicity of nine prickly pear (Opuntia spp.) juices. Plant Foods Hum Nutr, 64(2), 146-152. https://doi.org/10.1007/s11130-009-0117-0
Dumanoğlu, Z., Güzel, Ü., & Çakır, A. (2020). Doğu Akdeniz bölgesinde yetişen dikenli incir (Opuntia ficus-indica L.) tohumlarının bazı fiziksel özelliklerinin belirlenmesi üzerine bir araştırma. Türk Doğa ve Fen Dergisi, 9(Özel Sayı), 28-33.
El-Beltagi, H. S., Mohamed, H. I., Elmelegy, A. A., Eldesoky, S. E., & Safwat, G. (2019). Phytochemical screening, antimicrobial, antiaxidant, anticancer activities and nutritional values of cactus (Opuntia ficus indica) pulp and peel. Fresenius Environmental Bulletin, 28(2a), 1545-1562. ://WOS:000461270800063
El Mannoubi, I. (2021). Effect of extraction solvent on phenolic composition, antioxidant and antibacterial activities of skin and pulp of Tunisian red and yellow–orange Opuntia ficus indica fruits. Journal of Food Measurement and Characterization, 15(1), 643-651.
Fatullayev, H., Pasayeva, L., Celik, I., Ince, U., & Tugay, O. (2023). Phytochemical composition, in vitro antimicrobial, antioxidant, enzyme ınhibition activities, in silico molecular docking dynamics simulations of Centaurea lycaonica: a computational and experimental approach. ACS omega, 8(25), 22854-22865. https://doi.org/10.1021/ acsomega.3c01819
Ferrari, M., Fornasiero, M. C., & Isetta, A. M. (1990). MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods, 131(2), 165-172. https://doi.org/10.1016/0022-1759(90)90187-z
Floegel, A., Kim, D. O., Chung, S. J., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of food composition and analysis, 24(7), 1043-1048. https://doi.org/10.1016/j.jfca.2011.01.008
Frati, A. C., Jimenez, E., & Ariza, C. R. (1990). Hypoglycemic effect of Opuntia-ficus-indica in non-insulin-dependent Diabetes-Mellitus patients. Phytotherapy Research, 4(5), 195-197. https://doi.org/DOI 10.1002/ptr.2650040507
Giraldo-Silva, L., Ferreira, B., Rosa, E., & Dias, A. C. P. (2023). Opuntia ficus-indica fruit: a systematic review of its phytochemicals and pharmacological activities. Plants (Basel), 12(3), 543. https://doi.org/10.3390/plants12030543
Gómez‐Maqueo, A., Ortega‐Hernández, É., Serrano‐Sandoval, S. N., Jacobo‐Velázquez, D. A., García‐Cayuela, T., Cano, M. P., & Welti‐Chanes, J. (2020). Addressing key features involved in bioactive extractability of vigor prickly pears submitted to high hydrostatic pressurization. Journal of food process engineering, 43(1), e13202. https://doi.org/ 10.1111/jfpe.13202
Gouws, C. A., D'Cunha, N. M., Georgousopoulou, E. N., Mellor, D. D., & Naumovski, N. (2019). The effect of different drying techniques on phytochemical content and in vitro antioxidant properties of Australian-grown prickly pears (Opuntia ficus indica). Journal of Food Processing and Preservation, 43(3), e13900. https://doi.org/ 10.1111/jfpp.13900
Güven, L., & Gülçin, İ. (2024). Determination of metabolic profiling by LC-MS/MS, evaluation of antioxidant activities, and enzyme inhibition effects of Helichrysum plicatum subsp. pseudopliacatum. KSÜ J. Agric Nat, 27(3), 501-514.
Hwang, S. H., Kang, I. J., & Lim, S. S. (2017). Antidiabetic effect of fresh nopal (Opuntia ficus-indica) in low-dose streptozotocin-induced diabetic rats fed a high-fat diet. Evid Based Complement Alternat Med, 2017, 4380721. https://doi.org/ 10.1155/2017/4380721
Ishtiaque, S., Naz, S., Siddiqi, R., Umer Abdullah, S., Khan, K., Ahmed, J., & Badaruddin, M. (2014). Antioxidant activities and total phenolics contents from extracts of Terminalia catappa, Carrisa carandas, and Opuntia ficus indica fruits. Recent Innovations in Chemical Engineering (Formerly Recent Patents on Chemical Engineering), 7(2), 106-112. https://doi.org/10.2174/2211334707666 150306 230804
Kaewnarin, K., Suwannarach, N., Kumla, J., & Lumyong, S. (2016). Phenolic profile of various wild edible mushroom extracts from Thailand and their antioxidant properties, anti-tyrosinase and hyperglycaemic inhibitory activities. Journal of Functional Foods, 27, 352-364. https://doi.org/ 10.1016/j.jff.2016.09.008
Karaboğa-Arslan, A. K., & Yerer, M. B. (2018). α-Chaconine and α-Solanine inhibit RL95-2 endometrium cancer cell proliferation by reducing expression of Akt (Ser473) and ERα (Ser167). Nutrients, 10(6), 672. https://doi.org/10.3390/ nu10060672
Keleş, H. (2023). Opuntia ficus-indica (L.) Miller Meyveleri Üzerinde Farmakognozik Çalışmalar. (Tez no: 780266). [Doktora Tezi, Hacettepe Üniversitesi, Sağlık Bilimleri Enstitüsü, Farmakognozi Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
Krishnaiah, D., Sarbatly, R., & Nithyanandam, R. (2011). A review of the antioxidant potential of medicinal plant species. Food and bioproducts processing, 89(C3), 217-233. https://doi.org/10.1016/j.fbp.2010.04.008
Manzur-Valdespino, S., Ramírez-Moreno, E., Arias-Rico, J., Jaramillo-Morales, O. A., Calderón-Ramos, Z. G., Delgado-Olivares, L., Córdoba-Díaz, M., Córdoba-Díaz, D., & Cruz-Cansino, N. D. (2020). Opuntia ficus-indica L. Mill residues—properties and application possibilities in food supplements. Applied Sciences, 10(9), 3260. https://doi.org/10.3390/app10093260
Masmoudi, R., Mersni, R., Achour, O., Smaali, I., & Maugard, T. (2024). Phenolic composition and anti-tyrosinase activities of extracts from Tunisian Opuntia ficus-indica flowers obtained by different extraction solvents. Chemistry Africa, 1-11. https://doi.org/10.1007/s42250-024-00892-8
Nanok, K., & Sansenya, S. (2020). Alpha-glucosidase, alpha-amylase, and tyrosinase inhibitory potential of capsaicin and dihydrocapsaicin. J Food Biochem, 44(1), e13099. https://doi.org/10.1111/jfbc.13099
Naselli, F., Tesoriere, L., Caradonna, F., Bellavia, D., Attanzio, A., Gentile, C., & Livrea, M. A. (2014). Anti-proliferative and pro-apoptotic activity of whole extract and isolated indicaxanthin from Opuntia ficus-indica associated with re-activation of the onco-suppressor p16(INK4a) gene in human colorectal carcinoma (Caco-2) cells. Biochem Biophys Res Commun, 450(1), 652-658. https://doi.org/10.1016/j.bbrc.2014.06.029
Paşayeva, L., Fatullayev, H., Celik, I., Unal, G., Bozkurt, N. M., Tugay, O., & Abdellattif, M. H. (2022). Evaluation of the chemical composition, antioxidant and antidiabetic activity of Rhaponticoides iconiensis flowers: effects on key enzymes linked to type 2 diabetes in vitro, in silico and on alloxan-induced diabetic rats in vivo. Antioxidants (Basel), 11(11), 2284. https:// doi.org/10.3390/antiox11112284
Paşayeva, L., Eruygur, N., Ayaz, F., Tugay, O., & Fatullayev, H. (2021). Assessment of the antioxidant and enzyme inhibition activities of Cousinia iconica with focus on phytochemical investigation by LC-MS/MS. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 155(6), 1178-1188. https://doi.org/10.1080/11263504.2020.1829736
Petersmann, A., Nauck, M., Muller-Wieland, D., Kerner, W., Muller, U. A., Landgraf, R., Freckmann, G., & Heinemann, L. (2018). Definition, classification and diagnosis of Diabetes Mellitus. Exp Clin Endocrinol Diabetes, 126(7), 406-410. https://doi.org/10.1055/a-0584-6223
Saenz, C. (2000). Processing technologies: an alternative for cactus pear (Opuntia spp.) fruits and cladodes. Journal of Arid Environments, 46(3), 209-225. https://doi.org/10.1006/jare.2000.0676
Serra, A. T., Poejo, J., Matias, A. A., Bronze, M. R., & Duarte, C. M. M. (2013). Evaluation of Opuntia spp. derived products as antiproliferative agents in human colon cancer cell line (HT29). Food Research International, 54(1), 892-901. https://doi.org/ 10.1016/j.foodres.2013.08.043
Siriwardhana, N., Shahidi, F., & Jeon, Y. J. (2006). Potential antioxidative effects of cactus pear fruit extract on radical scavenging and DNA damage reduction in human peripheral lymphocytes. Journal of food lipids, 13(4), 445-458. https://doi.org/DOI 10.1111/j.1745-4522.2006. 00065.x
Skelin, M., Rupnik, M., & Cencic, A. (2010). Pancreatic beta cell lines and their applications in Diabetes Mellitus Research. ALTEX-Alternatives to animal experimentation, 27(2), 105-113. https://doi.org/10.14573/altex.2010.2.105
Skyler, J. S. (2004). Diabetes mellitus: pathogenesis and treatment strategies. J Med Chem, 47(17), 4113-4117. https://doi.org/10.1021/jm0306273
Ydjedd, S., Chaalal, M., Bahri, S., Mokadem, S., & Radji, H. (2021). Antioxidant and α-amylase inhibition activities of prickly pears (Opuntia ficus indica L.) betalains extracts and application in yoghurt as natural colorants. J. Agroaliment. Processes Technol, 27(2), 140-150.
Zeghad, N., Ahmed, E., Belkhiri, A., Heyden, Y. V., & Demeyer, K. (2019). Antioxidant activity of Vitis vinifera, Punica granatum, Citrus aurantium and Opuntia ficus indica fruits cultivated in Algeria. Heliyon, 5(4), 1-19 e01575. https://doi.org/ 10.1016/j.heliyon.2019.e01575

Ayrıntılar

Birincil Dil

Türkçe

Konular

Farmasotik Botanik

Bölüm

Araştırma Makalesi

Yazarlar

Leyla Paşayeva ^*
0000-0003-3860-7222
Türkiye

Sena Kıcalı
0009-0008-6179-6387
Türkiye

Ayşe Kübra Karaboğa Arslan
0000-0002-4689-0657
Türkiye

Erken Görünüm Tarihi

15 Eylül 2024

Yayımlanma Tarihi

25 Aralık 2024

Gönderilme Tarihi

2 Nisan 2024

Kabul Tarihi

15 Ağustos 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: Ek Sayı 1 (Suppl 1)

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1463666

IZ

https://izlik.org/JA53GA24XE

Kaynak Göster

RIS / Bibtex

APA

Paşayeva, L., Kıcalı, S., & Karaboğa Arslan, A. K. (2024). Opuntia ficus-indica (L.) Mill. Bitkisinden Hazırlanan Farklı Ekstrelerin Antidiyabetik, Antitrozinaz, Antioksidan ve Sitotoksik Etkisinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1), 185-193. https://doi.org/10.18016/ksutarimdoga.vi.1463666

Investigation of the Antidiabetic, Antitrosinase, Antioxidant, and Cytotoxic Effects of Different Extracts Prepared from Opuntia ficus-indica (L.) Mill. plant

Abstract

Keywords

Opuntia ficus-indica (L.) Mill. Bitkisinden Hazırlanan Farklı Ekstrelerin Antidiyabetik, Antitrozinaz, Antioksidan ve Sitotoksik Etkisinin Araştırılması

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

e-ISSN: 2619-9149