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Genotypic Responses of Some Besni Pepper (Capsicum annuum L.) Genotypes to Anther Culture

Yıl 2025, Cilt: 28 Sayı: 1, 114 - 131

Mirac Şahin , Halit Yetişir , Hasan Pinar , Alim Aydın

https://doi.org/10.18016/ksutarimdoga.vi.1555480

Öz

In this study, the response of Besni pepper (Capsicum annuum L), one of the local varieties of Turkey, to anther culture was determined. A total of 26 Besni pepper genotypes and 3 control cultivars were examined for their response to anther culture. One hundred and fifty anthers from each pepper genotype were cultured under in vitro conditions. A significant difference (0.0%-45.3%) was found among the genotypes in terms of response to anther culture. The highest embryo formation rate was found in genotype B11 with 45.3% (63 embryos) and the highest transformation rate to plant was found in genotype B15 with 30 plants (68%). Compared to control varieties, Besni pepper genotypes produced significantly more embryos. All genotypes except two out of 26 genotypes used produced more or less (1-68) embryos. B4, B10, B11, B12, B15, and G6 genotypes produced more than 25% of embryos and were separated from the control and other genotypes. The average embryo formation rate of the genotypes collected from Besni and Gölbaşı districts was 13% and 7%, respectively, while the embryo formation rate of the control varieties was only 0.2%. It was concluded that the Besni pepper population was highly responsive to androgenetic haploid. The highly responsive genotypes that form high embryos such as B4, B10, B11, B12, B15, and G6 have the potential to be used in developing new breeding lines and in studies investigating the genetics of anther culture.

Anahtar Kelimeler

Pepper Germplasm Androgenesis Embryo Inbred line

Proje Numarası

FYL-2021-10960

Kaynakça

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Bazı Besni Biberi (Capsicum annuum L.) Genotiplerinin Anter Kültürüne Karşı Tepkileri

Yıl 2025, Cilt: 28 Sayı: 1, 114 - 131

Mirac Şahin , Halit Yetişir , Hasan Pinar , Alim Aydın

https://doi.org/10.18016/ksutarimdoga.vi.1555480

Öz

Bu çalışmada, Türkiye'nin yerel çeşitlerinden biri olan Besni biberinin (Capsium annuum L.) anter kültürüne cevabı belirlenmiştir. Toplam 26 Besni biber genotipi ve 3 kontrol çeşidi anter kültürüne cevapları açısından incelenmiştir. Her biber genotipinden 150 anter in vitro koşullarda kültüre alınmıştır. Anter kültürüne cevap açısından genotipler arasında anlamlı bir fark (%0,0-%45,3) bulunmuştur. En yüksek embriyo oluşum oranı %45,3 (63 embriyo) ile B11 genotipinde, en yüksek bitkiye dönüşüm oranı ise 30 bitki (%68) ile B15 genotipinde bulunmuştur. Kontrol çeşitleriyle karşılaştırıldığında, Besni biber genotipleri anlamlı olarak daha fazla embriyo üretmiştir. Kullanılan 26 genotipten ikisi hariç tüm genotipler daha fazla veya daha az (1-68) embriyo üretmiştir. B4, B10, B11, B12, B15 ve G6 genotipleri %25'ten fazla embriyo üretmiş ve kontrol ve diğer genotiplerden ayrılmıştır. Besni ve Gölbaşı ilçelerinden toplanan genotiplerin ortalama embriyo oluşum oranı sırasıyla %13 ve %7 iken, kontrol çeşitlerinin embriyo oluşum oranı sadece %0,2'dir. Besni biber popülasyonunun androgenetik haploide oldukça duyarlı olduğu sonucuna varılmıştır. Yüksek embriyo oluşturan B4, B10, B11, B12, B15 ve G6 gibi oldukça duyarlı genotipler yeni ıslah hatlarının geliştirilmesinde ve anter kültürünün genetiğinin araştırıldığı çalışmalarda kullanılma potansiyeline sahiptir.

Anahtar Kelimeler

Peper Genetik kaynak Androgenesis Embriyo Saf hat

Etik Beyan

Makale yazarları, bu makalede sunulan verilerin, bilgilerin ve dokümanların akademik ve etik kurallar çerçevesinde elde edildiğini, tüm bilgi, belge, değerlendirme ve sonuçlarının bilimsel etik ve ahlak kurallarına uygun olarak sunulduğunu beyan ederler

Destekleyen Kurum

Erciyes University Scientific Research Projects Coordinating Office

Proje Numarası

FYL-2021-10960

Teşekkür

The authors would like to thank Erciyes University Scientific Research Coordination Unit for its financial support and Erciyes University Agricultural Research and Application Center for the space and material support it provided.

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  • Supena, E.D.J., Suharsono, S., Jacobsen, E & Custers, J.B.M. (2006). Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.). Plant Cell Reports 25(1), 1–10. 10.1007/s00299-005-0028-y
  • Taskin, H., Buyukalaca, S., Keles, D., & Ekbic, E. (2011). Induction of microspore derived embryos by anther culture in selected pepper genotypes. African Journal of Biotechnology 10(75), 17116-17121. 10.5897/AJB11.2023
  • Testillano, P.S., Coronado, M.J., Thierry, A.M., Matthys-Rochon, E., & Risuen˜o, M.C. (2010). In situ detection of Esr proteins secretion during maize microspore embryogenesis and their secretion blockage show effects on the culture progression. Functional Plant Biology 37, 985-994. https://doi.org/10.1071/FP10066
  • Testillano, P. S. (2019). Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement. J. Exp. Bot. 70, 2965–2978. doi: 10.1093/jxb/ery464
  • Velasco M.H., & Mattsson, A. (2020). Light shock stress after outdoor sunlight exposure in seedlings of Picea abies (L.) Karst. and Pinus sylvestris L. pre-cultivated under LEDs—possible mitigation treatments and their energy consumption. Forests 11, 354; doi:10.3390/f11030354.
  • Vural, H., Eşiyok, D., & Duman, İ. (2000). Cultivated Vegetables (Vegetable Growing), Ege University, Faculty of Agriculture, Department of Horticulture, Bornova, Izmir.
  • Wang, Y.Y., Sun, C.S., Wang, C.C., & Chien, N.J. (1973). The induction of pollen plantlets of triticale and Capsicum annuum anther culture. Scientia Sinica 16(1): 147-15.10.1186/s40529-023-00408-6SciSin
  • Xie, L., Wang, X., Peng, M., Meng, F., Zhou, Y., Chen. L., Liu, L., Gao, Y., & Guo, Y. (2014). isolation and detection of differential genes in hot pepper (Capsicum annuum L.) after space flight using AFLP markers. Biochemical Systematics and Ecology 57, 27-32. https://doi.org/10.1016/j.bse.2014.07.020
  • Yılmaz, G., & Karan, T. (2023). Current Applications and Evaluations in Plant Biotechnology. Livre de Lyon, Liyon.
  • Zeng, A., Song, L., Cui, Y., & Yan, J. (2017). Reduced ascorbate and reduced glutathione improve embryogenesis in broccoli microspore culture. South African Journal of Botany 109, 275–280. https://doi.org/10.1016/j.sajb.2017.01.005
Toplam 97 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Biyoteknolojisi
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Mirac Şahin 0000-0002-7699-6380

Halit Yetişir 0000-0001-6955-9513

Hasan Pinar 0000-0002-0811-8228

Alim Aydın 0000-0002-9424-5556

Proje Numarası FYL-2021-10960
Erken Görünüm Tarihi 30 Ocak 2025
Yayımlanma Tarihi
Gönderilme Tarihi 25 Eylül 2024
Kabul Tarihi 3 Ocak 2025
Yayımlandığı Sayı Yıl 2025Cilt: 28 Sayı: 1

Kaynak Göster

APA Şahin, M., Yetişir, H., Pinar, H., Aydın, A. (2025). Genotypic Responses of Some Besni Pepper (Capsicum annuum L.) Genotypes to Anther Culture. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(1), 114-131. https://doi.org/10.18016/ksutarimdoga.vi.1555480
 Kapak Resmi İndir

21082



2022-JIF = 0.500

2022-JCI = 0.170

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      Yılda 6 sayı yayınlanır. (Published 6 times a year)


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