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Antigenotoxic Effects of Stachys viscosa (Lamiaceae), a potential medicinal plant in Drosophila melanogaster

Yıl 2025, Cilt: 28 Sayı: 1, 53 - 61, 12.02.2025

Aygül Kılıç Karabulut , Mikail Açar

https://doi.org/10.18016/ksutarimdoga.vi.1574067

Öz

This research aimed to examine the anti-genotoxic properties of Stachys viscosa, a plant of the Lamiaceae family, which has medicinal and aromatic properties and is widely used in cosmetics, food, and pharmaceutical industries. The Drosophila wing somatic mutation and recombination test (SMART), an in vivo tool for evaluating somatic mutation and recombination, was employed. The anti-genotoxic effect of the S. viscosa plant against mitomycin C (MMC) mutagen was investigated. The study evaluated doses of 5 mg, 15 mg, 45 mg, and 60 mg of S. viscosa, concluding that doses of 45 mg and 60 mg effectively mitigated DNA damage induced by MMC. At doses of 45 mg and 65 mg, the total number of spots caused by MMC was suppressed by 8.21% and 13.17%, respectively. The data collected indicate that the S. viscosa plant possesses certain anti-genotoxic properties.

Anahtar Kelimeler

Antigenotoxicty Drosophila melanogaster SMART Stachys viscosa Mitomycin C (MMC)

Kaynakça

  • Al Snafi, A.E. (2013). The pharmaceutical importance of Althea officinalis and Althea rosea: A Review. International Journal of PharmTech Research, 5, 1378–1385.
  • Boldbaatar, D., El-Seedi, H.R., Findakly, M., Jabri, S., Javzan, B., Choidash, B., Göransson, U., & Hellman, B. (2014). Antigenotoxic and antioxidant effects of the Mongolian medicinal plant Leptopyrum fumarioides (L): An in vitro study. Journal of Ethnopharmacology, 155(1), 599-606.
  • Çelik, H., & Uysal, H. (2023). ‘Skualen’ triterpeninin somatik mutasyonlar üzerine etkisinin Drosophila melanogaster’de in vivo kanat benek testi ile araştırılması. KSÜ Tarım ve Doğa Dergisi, 26(3), 477-486.
  • Çetinkaya, A.Y., & Yurtsever, S. (2021). Somatic mutations and recombination test in Drosophila melanogaster used for investigating the genotoxicity of some food additives. International Journal of Agriculture, Environment and Food Science, 5(1), 65-73.
  • Duman, H., Kartal, M., Altun, L., Demirci, B., & Başer, K.H.C. (2005). The essential oil of Stachys laetivirens Kotschy & Boiss. ex Rech. fil., endemic in Turkey. Flavour and Fragrance Journal, 20(1), 48-50.
  • El-Hefny, I., Hozayen, W., AlSenosy, N., Basal, W., Ahmed, A., & Diab, A. (2020). Evaluation of genotoxicity of three food preservatives in Drosophila melanogaster using Smart and Comet Assays. Journal of Microbiology, Biotechnology and Food Sciences, 10(1), 38-41.
  • Gezici, S., Turkmen, M., & Karahan, F. (2024). Exploring the anti-cancer properties of essential oils from some Lamiaceae species against human cancer cells with multivariate analysis. South African Journal of Botany, 166, 287-296.
  • Graf, U., Würgler, F.E., Katz, A.J., Frei, H., Juon, H., Hall, C.B., & Kale, P.G. (1984). Somatic mutation test in Drosophila melanogaster. Environmental Mutagenesis, 6, 153–88.
  • Idaomar, M., El Hamss, R., Bakkali, F., Mezzoug, N., Zhiri, A., Baudoux, D., Serrano, M., Liemans, V., & Moraga, A. (2002). Genotoxicity and antigenottoxicity of some essential oils evaluated by wing spot test of Drosophila melanogaster. Mutation Research, 513, 61-68.
  • Karabulut, A.K., & Yesilada, E. (2014). Genotoxicity testing of tributyltin and methidathion in Drosophila melanogaster using the wing somatic mutation and recombination test. Fresenius Environmental Bulletin, 23, 3476-3481.
  • Kaya, G.Ö. (2013). Althaea officinalis L. Bitkisinin Fitoterapi Yönünden Değerlendirilmesi. (Tez no 375080) [Master's thesis, Gazi Üniversitesi, Ankara]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Khalighi, N., Jabbari-Azad, F., Barzegar-Amini, M., Tavakkol-Afshari, J., Layegh, P., & Salari, R. (2021). Impact of Althaea officinalis extract in patients with atopic eczema: a double-blind randomised controlled trial. International Journal of Phytomedicine and Phytotherapy, 7, 73.
  • Kilic, D.D., Ayar, A., Baskan, C., & Yildirim, T. (2018). Genotoxic and antigenotoxic effects of some plant species of Lamiaceae family. Avrupa Bilim ve Teknoloji Dergisi, 14, 348-352. https://doi.org/10.31590/ejosat.430874
  • Kolumbayeva, S.Zh., Lovinskaya, A.V., Arutyunyan, T.Sh., Maygozhina, D.K., Suvorova, M.A., & Abilev, S.K. (2022). Antimutagenic activity of alcoholic extracts of medicinal herbs Mentha piperita L. and Thymus vulgaris L. family Lamiaceae. Экология сериясы, 1(70). https://bulletin-ecology.kaznu.kz https://doi.org/10.26577/EJE.2022.v70.i1.03
  • Laohavechvanich, P., Kangsadalampai, K., Tirawanchai, N., & Ketterman, A.-J. (2006). Effect of different Thai traditional processing of various hot chili peppers on urethane-induced somatic mutation and recombination in Drosophila melanogaster: Assessment of the role of glutathione transferase activity. Food and Chemical Toxicology, 44(8), 1348-1354.
  • Rencuzogullari, E., Yildiz, A.M., & Buyukleyla, M. (2012). The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions. Cytotechnology 64, 83–94 https://doi.org/10.1007/s10616-011-9396
  • Niikawa, M., & Nagase, H. (2007). Effect of aspirin on DNA damage induced by MMC in Drosophila. Biomedicine & Pharmacotherapy, 61(5), 250-253.
  • Nilofar, N., Zengin, G., Acar, M., Bouyayha, A., Youssra, A., Eldahshan, O., ... & Fahmy, N. (2024). Assessing the chemical composition, antioxidant and enzyme inhibitory effects of Pentapleura subulifera and Cyclotrichium glabrescens extracts. Chemistry & Biodiversity, 21(2), e202301651.
  • Önen, Ö., Kılıçle, P. A., & Doğan, A. N. C. (2017). Baharat olarak kullanılan bazı bitki ekstraktlarının memeliler üzerindeki genotoksik ve antigenotoksik etkileri. Kafkas Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(2), 103-115.
  • Öz, S., Sarıkaya, Z. N., Larçın, Ö., & Sarıkaya, R. (2024). Investigation of the genotoxic effect of fluoxetine hydrochloride in Drosophila melanogaster. KSÜ Tarım ve Doğa Dergisi, 27(2), 316-324.
  • Rasgele, P. G., & Dulger, G. (2021). Chemical compositions and antimutagenic effects of ethanolic extracts of Stachys thirkei and Stachys annua subsp. annua using the Ames assay. Pharmaceutical Chemistry Journal, 54, 1255–1262. https://doi.org/10.1007/s11094-021-02351
  • Rencuzoğulları, E., Yıldız, A. M., & Büyükleyla, M. (2012). The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions. Cytotechnology, 64, 83–94. https://doi.org/10.1007/s10616-011-9396
  • Rincon, J. G., & Graf, U. (1995). Drosophila melanogaster somatic mutation and recombination test as a biomonitor. Biomonitors and Biomarkers as Indicators of Environmental Change, Plenum Press, New York, 169-179.
  • Sarıkaya, R., & Çakır, Ş. (2005). Genotoxicity testing of four food preservatives and their combination in Drosophila melanogaster. Environmental Toxicology and Pharmacology, 20(3), 424-430.
  • Satıl, F., & Açar, M. (2020). Ethnobotanical use of Stachys L. (Lamiaceae) taxa in Turkey. International Journal of Nature and Life Sciences, 4(2), 66-86.
  • Sevindik, N., & Rencuzoğulları, E. (2013). The genotoxic and antigenotoxic effects of Salvia fruticosa leaf extract in human blood lymphocytes. Drug and Chemical Toxicology, 37(3), 295–302. https://doi.org/10.3109/01480545.2013.851689
  • Shabab, S., Gholamnezhad, Z., & Mahmoudabady, M. (2021). Protective effects of medicinal plants against diabetes-induced cardiac disorder: A review. Journal of Ethnopharmacology, 265, 113328.
  • S-Radak, M., & Andjelkovic, M. (2016). Studying genotoxic and antimutagenic effects of plant extracts in Drosophila test systems. Botanica Serbica, 40(1), 21-28.
  • Stavropoulou, L. S., Efthimiou, I., Giova, L., Manoli, C., Sinou, P. S., Zografidis, A., Lamari, F. N., Vlastos, D., Dailianis, S., & Antonopoulou, M. (2024). Phytochemical profile and evaluation of the antioxidant, cyto-genotoxic, and antigenotoxic potential of Salvia verticillata hydromethanolic extract. Plants, 13, 731. https://doi.org/10.3390/plants13050731
  • Tomou, E. M., Barda, C., & Skaltsa, H. (2020). Genus Stachys: A review of traditional uses, phytochemistry, and bioactivity. Medicines, 7(10), 63.
  • Vasanthi, H. R., ShriShriMal, N., & Das, D. K. (2012). Retraction notice: Phytochemicals from plants to combat cardiovascular disease. Current Medicinal Chemistry, 19(14), 2242–2251.
  • Yalçın, B., Güneş, M., Burgazlı, A. Y., Tagorti, G., Golal, E., & Kaya, B. (2024). Investigation of genotoxic and development effects of tetramethrin on Drosophila melanogaster. KSÜ Tarım ve Doğa Dergisi, 27(2), 304-315.
  • Zor, M., & Aslan, E. L. (2020). Assessment of in vitro antigenotoxic effect of Nigella sativa oil. Turkish Journal of Pharmaceutical Sciences, 17(1), 115-118.

Potansiyel tıbbi bir bitki olan Stachys viscosa'nın (Lamiaceae) Drosophila melanogaster'da Antigenotoksik Etkileri

Yıl 2025, Cilt: 28 Sayı: 1, 53 - 61, 12.02.2025

Aygül Kılıç Karabulut , Mikail Açar

https://doi.org/10.18016/ksutarimdoga.vi.1574067

Öz

Bu araştırmada tıbbi ve aromatik özelliklere sahip olan, kozmetik, gıda ve ilaç endüstrilerinde yaygın olarak kullanılan Lamiaceae familyasına ait bir bitki olan Stachys viscosa'nın anti-genotoksik özelliklerinin incelenmesi amaçlanmıştır. Somatik mutasyon ve rekombinasyonu değerlendirmek için in vivo bir test olan Drosophila kanat somatik mutasyon ve rekombinasyon testi (SMART) kullanıldı. Yapılan çalışmada, S. viscosa bitkisinin Mitomisin C (MMC) mutajenine karşı anti-genotoksik etkisi araştırılmıştır. Çalışmada S. viscosa'nın 5 mg, 15 mg, 45 mg ve 60 mg'lık dozları değerlendirildi ve 45 mg ve 60 mg'lık dozların MMC'nin neden olduğu DNA hasarını etkili bir şekilde azalttığı saptandı. Araştırma neticesinde 45 mg ve 65 mg'lık dozlarda, MMC'nin neden olduğu toplam benek sayısının sırasıyla %8,21 ve %13,17 oranında baskılandığı tespit edilmiştir. Elde edilen veriler, S. viscosa bitkisinin belirli anti-genotoksik özelliklere sahip olduğunu göstermektedir.

Anahtar Kelimeler

Antigenotoksisite Drosophila melanogaster SMART Stachy viscosa Mitomisin C (MMC)

Kaynakça

  • Al Snafi, A.E. (2013). The pharmaceutical importance of Althea officinalis and Althea rosea: A Review. International Journal of PharmTech Research, 5, 1378–1385.
  • Boldbaatar, D., El-Seedi, H.R., Findakly, M., Jabri, S., Javzan, B., Choidash, B., Göransson, U., & Hellman, B. (2014). Antigenotoxic and antioxidant effects of the Mongolian medicinal plant Leptopyrum fumarioides (L): An in vitro study. Journal of Ethnopharmacology, 155(1), 599-606.
  • Çelik, H., & Uysal, H. (2023). ‘Skualen’ triterpeninin somatik mutasyonlar üzerine etkisinin Drosophila melanogaster’de in vivo kanat benek testi ile araştırılması. KSÜ Tarım ve Doğa Dergisi, 26(3), 477-486.
  • Çetinkaya, A.Y., & Yurtsever, S. (2021). Somatic mutations and recombination test in Drosophila melanogaster used for investigating the genotoxicity of some food additives. International Journal of Agriculture, Environment and Food Science, 5(1), 65-73.
  • Duman, H., Kartal, M., Altun, L., Demirci, B., & Başer, K.H.C. (2005). The essential oil of Stachys laetivirens Kotschy & Boiss. ex Rech. fil., endemic in Turkey. Flavour and Fragrance Journal, 20(1), 48-50.
  • El-Hefny, I., Hozayen, W., AlSenosy, N., Basal, W., Ahmed, A., & Diab, A. (2020). Evaluation of genotoxicity of three food preservatives in Drosophila melanogaster using Smart and Comet Assays. Journal of Microbiology, Biotechnology and Food Sciences, 10(1), 38-41.
  • Gezici, S., Turkmen, M., & Karahan, F. (2024). Exploring the anti-cancer properties of essential oils from some Lamiaceae species against human cancer cells with multivariate analysis. South African Journal of Botany, 166, 287-296.
  • Graf, U., Würgler, F.E., Katz, A.J., Frei, H., Juon, H., Hall, C.B., & Kale, P.G. (1984). Somatic mutation test in Drosophila melanogaster. Environmental Mutagenesis, 6, 153–88.
  • Idaomar, M., El Hamss, R., Bakkali, F., Mezzoug, N., Zhiri, A., Baudoux, D., Serrano, M., Liemans, V., & Moraga, A. (2002). Genotoxicity and antigenottoxicity of some essential oils evaluated by wing spot test of Drosophila melanogaster. Mutation Research, 513, 61-68.
  • Karabulut, A.K., & Yesilada, E. (2014). Genotoxicity testing of tributyltin and methidathion in Drosophila melanogaster using the wing somatic mutation and recombination test. Fresenius Environmental Bulletin, 23, 3476-3481.
  • Kaya, G.Ö. (2013). Althaea officinalis L. Bitkisinin Fitoterapi Yönünden Değerlendirilmesi. (Tez no 375080) [Master's thesis, Gazi Üniversitesi, Ankara]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Khalighi, N., Jabbari-Azad, F., Barzegar-Amini, M., Tavakkol-Afshari, J., Layegh, P., & Salari, R. (2021). Impact of Althaea officinalis extract in patients with atopic eczema: a double-blind randomised controlled trial. International Journal of Phytomedicine and Phytotherapy, 7, 73.
  • Kilic, D.D., Ayar, A., Baskan, C., & Yildirim, T. (2018). Genotoxic and antigenotoxic effects of some plant species of Lamiaceae family. Avrupa Bilim ve Teknoloji Dergisi, 14, 348-352. https://doi.org/10.31590/ejosat.430874
  • Kolumbayeva, S.Zh., Lovinskaya, A.V., Arutyunyan, T.Sh., Maygozhina, D.K., Suvorova, M.A., & Abilev, S.K. (2022). Antimutagenic activity of alcoholic extracts of medicinal herbs Mentha piperita L. and Thymus vulgaris L. family Lamiaceae. Экология сериясы, 1(70). https://bulletin-ecology.kaznu.kz https://doi.org/10.26577/EJE.2022.v70.i1.03
  • Laohavechvanich, P., Kangsadalampai, K., Tirawanchai, N., & Ketterman, A.-J. (2006). Effect of different Thai traditional processing of various hot chili peppers on urethane-induced somatic mutation and recombination in Drosophila melanogaster: Assessment of the role of glutathione transferase activity. Food and Chemical Toxicology, 44(8), 1348-1354.
  • Rencuzogullari, E., Yildiz, A.M., & Buyukleyla, M. (2012). The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions. Cytotechnology 64, 83–94 https://doi.org/10.1007/s10616-011-9396
  • Niikawa, M., & Nagase, H. (2007). Effect of aspirin on DNA damage induced by MMC in Drosophila. Biomedicine & Pharmacotherapy, 61(5), 250-253.
  • Nilofar, N., Zengin, G., Acar, M., Bouyayha, A., Youssra, A., Eldahshan, O., ... & Fahmy, N. (2024). Assessing the chemical composition, antioxidant and enzyme inhibitory effects of Pentapleura subulifera and Cyclotrichium glabrescens extracts. Chemistry & Biodiversity, 21(2), e202301651.
  • Önen, Ö., Kılıçle, P. A., & Doğan, A. N. C. (2017). Baharat olarak kullanılan bazı bitki ekstraktlarının memeliler üzerindeki genotoksik ve antigenotoksik etkileri. Kafkas Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10(2), 103-115.
  • Öz, S., Sarıkaya, Z. N., Larçın, Ö., & Sarıkaya, R. (2024). Investigation of the genotoxic effect of fluoxetine hydrochloride in Drosophila melanogaster. KSÜ Tarım ve Doğa Dergisi, 27(2), 316-324.
  • Rasgele, P. G., & Dulger, G. (2021). Chemical compositions and antimutagenic effects of ethanolic extracts of Stachys thirkei and Stachys annua subsp. annua using the Ames assay. Pharmaceutical Chemistry Journal, 54, 1255–1262. https://doi.org/10.1007/s11094-021-02351
  • Rencuzoğulları, E., Yıldız, A. M., & Büyükleyla, M. (2012). The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extract in human lymphocytes using microsomal fractions. Cytotechnology, 64, 83–94. https://doi.org/10.1007/s10616-011-9396
  • Rincon, J. G., & Graf, U. (1995). Drosophila melanogaster somatic mutation and recombination test as a biomonitor. Biomonitors and Biomarkers as Indicators of Environmental Change, Plenum Press, New York, 169-179.
  • Sarıkaya, R., & Çakır, Ş. (2005). Genotoxicity testing of four food preservatives and their combination in Drosophila melanogaster. Environmental Toxicology and Pharmacology, 20(3), 424-430.
  • Satıl, F., & Açar, M. (2020). Ethnobotanical use of Stachys L. (Lamiaceae) taxa in Turkey. International Journal of Nature and Life Sciences, 4(2), 66-86.
  • Sevindik, N., & Rencuzoğulları, E. (2013). The genotoxic and antigenotoxic effects of Salvia fruticosa leaf extract in human blood lymphocytes. Drug and Chemical Toxicology, 37(3), 295–302. https://doi.org/10.3109/01480545.2013.851689
  • Shabab, S., Gholamnezhad, Z., & Mahmoudabady, M. (2021). Protective effects of medicinal plants against diabetes-induced cardiac disorder: A review. Journal of Ethnopharmacology, 265, 113328.
  • S-Radak, M., & Andjelkovic, M. (2016). Studying genotoxic and antimutagenic effects of plant extracts in Drosophila test systems. Botanica Serbica, 40(1), 21-28.
  • Stavropoulou, L. S., Efthimiou, I., Giova, L., Manoli, C., Sinou, P. S., Zografidis, A., Lamari, F. N., Vlastos, D., Dailianis, S., & Antonopoulou, M. (2024). Phytochemical profile and evaluation of the antioxidant, cyto-genotoxic, and antigenotoxic potential of Salvia verticillata hydromethanolic extract. Plants, 13, 731. https://doi.org/10.3390/plants13050731
  • Tomou, E. M., Barda, C., & Skaltsa, H. (2020). Genus Stachys: A review of traditional uses, phytochemistry, and bioactivity. Medicines, 7(10), 63.
  • Vasanthi, H. R., ShriShriMal, N., & Das, D. K. (2012). Retraction notice: Phytochemicals from plants to combat cardiovascular disease. Current Medicinal Chemistry, 19(14), 2242–2251.
  • Yalçın, B., Güneş, M., Burgazlı, A. Y., Tagorti, G., Golal, E., & Kaya, B. (2024). Investigation of genotoxic and development effects of tetramethrin on Drosophila melanogaster. KSÜ Tarım ve Doğa Dergisi, 27(2), 304-315.
  • Zor, M., & Aslan, E. L. (2020). Assessment of in vitro antigenotoxic effect of Nigella sativa oil. Turkish Journal of Pharmaceutical Sciences, 17(1), 115-118.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Entomoloji
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Aygül Kılıç Karabulut 0000-0003-3452-8885

Mikail Açar 0000-0003-3848-5798

Erken Görünüm Tarihi 1 Şubat 2025
Yayımlanma Tarihi 12 Şubat 2025
Gönderilme Tarihi 26 Ekim 2024
Kabul Tarihi 23 Aralık 2024
Yayımlandığı Sayı Yıl 2025Cilt: 28 Sayı: 1

Kaynak Göster

APA Kılıç Karabulut, A., & Açar, M. (2025). Antigenotoxic Effects of Stachys viscosa (Lamiaceae), a potential medicinal plant in Drosophila melanogaster. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(1), 53-61. https://doi.org/10.18016/ksutarimdoga.vi.1574067
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