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Investigation of the Anticancer Effects on Human Cervical Cancer (HeLa) Cell Line and the DNA Protective Activities of Traditional Plants from Ordu Province (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum)

Yıl 2025, Cilt: 28 Sayı: 4, 945 - 954

Dr. Bülent Kaynak , Gülizar Aydoğdu , Zeynep Kolören , Onur Kolören

https://doi.org/10.18016/ksutarimdoga.vi.1651205

Öz

This study investigates the protective potential of ethanolic extracts of local plants from Ordu province, Brassica oleracea var. viridis, Smilax excelsa, and Conium maculatum, against DNA damage and their anticancer activities in HeLa (cervix adenocarcinoma) cells. The extracts were prepared at varying concentrations, and their anticancer effects were evaluated using the 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) assay in HeLa cells. The ability of these extracts to prevent hydroxyl radical (-OH)-induced damage to pBR322 plasmid DNA was also examined. MTT assay results showed that cell viability decreased in a dose-dependent manner when treated with different concentrations of the extracts. The approximate IC50 values for S. excelsa, B. oleracea var. viridis, and C. maculatum extracts at 24 hours were determined to be 3.88, 2.20, and 1.48 mg/mL, respectively, while at 48 hours, these values were 1.82, 1.13, and 0.39 mg/mL. Furthermore, the extracts demonstrated strong protective activity at various concentrations (20, 10, and 5 mg/mL) in preventing OH-induced DNA damage. These findings provide preliminary data on the DNA protective and anticancer effects of ethanol extracts of S. excelsa, B. oleracea var. viridis, and C. maculatum, indicating that future studies should focus on identifying the active compounds responsible for these effects and investigating their therapeutic potential in more detail.

Anahtar Kelimeler

[Brassica oleracea var. viridis Conium maculatum HeLa pBR322 Plasmid DNA Smilax excelsa

Kaynakça

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  • Alani, H., Ozaslan, M., Karagoz, I. D., & Simitcioglu, B. (2021). Investigation of Antimicrobial, DNA Protective and Cytotoxic Activity of Red Cabbage (Brassica Oleracea var. capitata f. rubra) Plant. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 12, 123-129. https://doi.org/10.55549/epstem.1038398.
  • Al-Snafi, A. E. (2016). Pharmacology and toxicology of Conium maculatum- A review. The Pharmaceutical and Chemical Journal, 3(2), 136-142. https://scholar.google.com/citations?hl=en&authuser=5&user=GMzIso 0AAAAJ.
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  • Arslan, A. K. K., Güngörenler, E., Paşayeva, L., Bozkurt, M., & Tugay, O. (2025). Centaurea lycaonica Boiss. & Heldr. Bitkisinin İnsan Servikal Kanser Hücre Hattında Sitotoksisitesinin MTT Testi ve xCELLigence Sistemi ile Değerlendirilmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(1), 47-52. https://doi.org/10.18016/ksutarimdoga.vi.1496499.
  • Bai, Y., Li, D., Zhou, T., Qin, N., Li, Z., Yu, Z., & Hua, H. (2016). Coumarins from the roots of Angelica dahurica with antioxidant and antiproliferative activities. Journal of Functional Foods, 20, 453-462. https://doi.org/10.1016/j.jff.2015.11.018.
  • Chaudhary, A., Choudhary, S., Sharma, U., Vig, A. P., Singh, B., & Arora, S. (2018). Purple head broccoli (Brassica oleracea var. italica plenck), a functional food crop for antioxidant and anticancer potential. Journal of food science and technology, 55, 1806-1815. https://doi.org/10.1007/s13197-018-3095-0.
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  • Filiz, B., Songul, K., Bostanlik, D., Gul, F., & Sibel, K. C. (2016). Anticancer effect of Ferulago mughlea Peşmen (Apiaceae) on cancer cell proliferation. Iranian Journal of Pharmaceutical Research, 15(3), 501. https://doi.org/10.22037/ijpr.2016.1882.
  • Golalipour, A., Mohammadi, A., Hosseinzadeh, S., Soltani, A., & Erfani-Moghadam, V. (2024). Synergistic cytotoxicity of olive leaf extract-loaded lipid nanocarriers combined with Newcastle disease virus against cervical cancer cells. Plos one, 19(8), e0308599. https://doi.org/10.1371/journal.pone.0308599.
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Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması

Yıl 2025, Cilt: 28 Sayı: 4, 945 - 954

Dr. Bülent Kaynak , Gülizar Aydoğdu , Zeynep Kolören , Onur Kolören

https://doi.org/10.18016/ksutarimdoga.vi.1651205

Öz

Bu çalışmada, Ordu iline ait yöresel bitkilerinden Brassica oleracea var. viridis, Smilax excelsa ve Conium maculatum’un etanolik özütlerinin DNA hasarına karşı koruyucu potansiyelleri ve insan serviks adenokarsinomu (HeLa) hücre hattına karşı antikanser aktiviteleri araştırılmıştır. Özütler, farklı konsantrasyonlarda hazırlanarak antikanser aktiviteleri, HeLa hücre hattında 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolyum bromid (MTT) testi ile değerlendirilmiştir. Özütlerin, hidroksil radikali (-OH) ile indüklenen pBR322 plazmid DNA hasarını engelleme potansiyelleri de araştırılmıştır. Özütlerin HeLa hücreleri ile yapılan MTT analizleri neticesinde, farklı konsantrasyonlarla yapılan uygulamalarda, hücre canlılığının doza bağlı olarak azaldığı gözlenmiştir. S. excelsa, B. oleracea var. viridis ve C. maculatum özütlerinin 24 saatteki yaklaşık IC50 değerleri sırasıyla 3.88, 2.20 ve 1.48 mg/mL, 48 saatteki yaklaşık IC50 değerleri ise sırasıyla 1.82, 1.13 ve 0.39 mg/mL olarak tespit edilmiştir. Ayrıca, -OH ile indüklenen DNA hasarını önleme açısından özütlerin farklı (20, 10 ve 5 mg/mL) konsantrasyonlarının güçlü koruyucu aktivite gösterdiği bulunmuştur.
Bu bulgular, S. excelsa, B. oleracea var. viridis ve C. maculatum etanolik özütlerinin DNA koruyucu ve antikanser etkilerine dair öncü veriler sunmakta olup, ileri çalışmalarda bu etkilerden sorumlu aktif bileşiklerin belirlenmesi ve terapötik potansiyellerinin detaylı olarak incelenmesi gerektiğini ortaya koymaktadır.

Anahtar Kelimeler

[Brassica oleracea var. viridis Conium maculatum HeLa pBR322 Plazmid DNA Smilax excelsa

Etik Beyan

Etik onay gerekmemektedir.

Kaynakça

  • Acharya, J. D., & Ghaskadbi, S. S. (2013). Protective effect of Pterostilbene against free radical mediated oxidative damage. BMC complementary and alternative medicine, 13, 1-10.https://doi.org/10.1186/1472-6882-13-238.
  • Al-Yassine, D., El Massri, N., Demircan, G., Bulut, G., Akin, D., & Tacer-Caba, Z. (2023). Total Antioxidant Potential, Total Phenolic Profile and Cytotoxic Activity Against Brain Cancer: Melocan and Galdirik. Food Technology and Biotechnology, 61(4), 475-484. https://doi.org/10.17113/ftb.61.04.23.8071.
  • Alani, H., Ozaslan, M., Karagoz, I. D., & Simitcioglu, B. (2021). Investigation of Antimicrobial, DNA Protective and Cytotoxic Activity of Red Cabbage (Brassica Oleracea var. capitata f. rubra) Plant. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 12, 123-129. https://doi.org/10.55549/epstem.1038398.
  • Al-Snafi, A. E. (2016). Pharmacology and toxicology of Conium maculatum- A review. The Pharmaceutical and Chemical Journal, 3(2), 136-142. https://scholar.google.com/citations?hl=en&authuser=5&user=GMzIso 0AAAAJ.
  • Aliyazicioglu, Y., Demir, S., Yaman, S. O., Sener, S. O., Demir, E. A., Aliyazicioglu, R., & Turan, I. (2019). Phytochemical analysis of Dorycnium pentaphyllum and its antiproliferative effect on cervix cancer cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 22, 365-373. https://doi.org/10.18016/ksutarimdoga.vi.579938.
  • Anonim, (2025a). Brassica oleracea. https://pfaf.org/user/plant.aspx?latinname= Brassica oleracea. (Alınma Tarihi: 20.02.2025).
  • Anonim, (2025b). Conium maculatum. http://194.27.225.161/yasin/tubives/index.php?sayfa=1&tax_id=4228. (Alınma Tarihi: 20.02.2025).
  • Arora, S., & Vig, A. P. (2015). Inhibition of DNA oxidative damage and antimutagenic activity by dichloromethane extract of Brassica rapa var. rapa L. seeds. Industrial Crops and Products, 74, 585-591. https://doi.org/10.1016/j.indcrop.2015.05.038.
  • Arslan, A. K. K., Güngörenler, E., Paşayeva, L., Bozkurt, M., & Tugay, O. (2025). Centaurea lycaonica Boiss. & Heldr. Bitkisinin İnsan Servikal Kanser Hücre Hattında Sitotoksisitesinin MTT Testi ve xCELLigence Sistemi ile Değerlendirilmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(1), 47-52. https://doi.org/10.18016/ksutarimdoga.vi.1496499.
  • Bai, Y., Li, D., Zhou, T., Qin, N., Li, Z., Yu, Z., & Hua, H. (2016). Coumarins from the roots of Angelica dahurica with antioxidant and antiproliferative activities. Journal of Functional Foods, 20, 453-462. https://doi.org/10.1016/j.jff.2015.11.018.
  • Chaudhary, A., Choudhary, S., Sharma, U., Vig, A. P., Singh, B., & Arora, S. (2018). Purple head broccoli (Brassica oleracea var. italica plenck), a functional food crop for antioxidant and anticancer potential. Journal of food science and technology, 55, 1806-1815. https://doi.org/10.1007/s13197-018-3095-0.
  • Chevalier, A. (1996). The Encyclopedia of Medicinal Plants. Dorling Kindersley Book, London, UK., ISBN-13: 9780789410672, Page: 227.
  • Chopra, R. N., Nayar, S. L., & Chopra, I. C. (1986). Glossary of Indian medicinal plants (including the supplement). Council of scientific and industrial research, New Delhi, 89, 77-100. https://doi.org/10.1016/0031-9422(93)85072-y.
  • Cinar, A. S., Bakar-Ates, F., & Onder, A. (2020). Seseli petraeum M. Bieb. (Apiaceae) Significantly inhibited cellular growth of A549 lung cancer cells through G0/G1 cell cycle arrest. Anais da Academia Brasileira de Ciências, 92(4), e20191533. https://doi.org/10.1590/0001-3765202020191533.
  • Fetter, B. Z., Dourado, D. M., Bogo, D., Matias, R., & Guterres, Z. R. (2022). Therapeutic potential of Smilax fluminensis ethanolic extract: antitumoral activity in murine melanoma cells. Molecular and Cellular Biochemistry, 1-9. https://doi.org/10.1007/s11010-021-04272-5.
  • Filiz, B., Songul, K., Bostanlik, D., Gul, F., & Sibel, K. C. (2016). Anticancer effect of Ferulago mughlea Peşmen (Apiaceae) on cancer cell proliferation. Iranian Journal of Pharmaceutical Research, 15(3), 501. https://doi.org/10.22037/ijpr.2016.1882.
  • Golalipour, A., Mohammadi, A., Hosseinzadeh, S., Soltani, A., & Erfani-Moghadam, V. (2024). Synergistic cytotoxicity of olive leaf extract-loaded lipid nanocarriers combined with Newcastle disease virus against cervical cancer cells. Plos one, 19(8), e0308599. https://doi.org/10.1371/journal.pone.0308599.
  • Gokcimen, S. S., Ipek, Y., Behcet, L., Demirtas, I., & Ozen, T. (2024). Isolation, characterization and evaluation of oxypeucedanin and osthol from local endemic Prangos aricakensis Behçet and Yapar root as antioxidant, enzyme inhibitory, antibacterial and DNA protection: molecular docking and DFT approaches. Journal of Biomolecular Structure and Dynamics, 1-18. https://doi.org/10.1080/07391102.2024.2303387.
  • Gyaneshwari, U., Singh, A. K., Pal, P., & Pandey, B. 15 Conium maculatum (Hemlock or Poison Hemlock). Exploring Poisonous Plants. 1st Edition. 2023. CRC Press. pp:16. eBook ISBN9781003308249. http://dx.doi.org/10.1201/b23017-17.
  • Hafidh, R. R., Abdulamir, A. S., Bakar, F. A., Jalilian, F. A., Jahanshiri, F., Abas, F., & Sekawi, Z. (2013). Novel anticancer activity and anticancer mechanisms of Brassica oleracea var. capitata f. rubra. European Journal of Integrative Medicine, 5(5), 450-464. https://doi.org/10.1016/j.eujim.2013.06.004.
  • Hussain, F., Unab, S., & Shahid, M. (2022). Exploring the Pharmacological Potential of Smilax china: Pharmacological Potential of Smilax china. Biological Sciences, 65(2), 135-144. https://doi.org/10.52763.PJSIR.BIOL.SCI.65.2.2022.135.144.
  • Kaynak, B., Koloren, Z., Karaman, U. (2019). Investigation of In Vitro Amoebicidal Activities of Trachystemon orientalis on Acanthamoeba castellanii Cysts and Trophozoites. Van Medical Journal, 26 (4): 483-490. https://doi.org/10.5505/vtd.2019.79926.
  • Kaynak, B., Koloren, Z., Ilhan, H., Ergun, S., & Aydogdu, G. (2025). Expression Analysis of Cyst Specific Protein (CSP21) and Cellulose Synthase II (CSII) Genes in Acanthamoeba castellanii Trophozoites Exposed to Silver Nanoparticles Conjugated with Elaeagnus umbellata. Acta Parasitologica, 70(1), 5. https://doi.org/10.1007/s11686-024-00947-3.
  • Khaligh, P., Salehi, P., Farimani, M. M., Ali-Asgari, S., Esmaeili, M. A., & Nejad-Ebrahimi, S. (2016). Bioactive compounds from Smilax excelsa L. Journal of the Iranian Chemical Society, 13, 1055-1059. https://doi.org/10.1007/s13738-016-0819-9.
  • Kim, M. J., Kim, H. J., Kim, M. G., Lee, S. H., & Jeon, B. G. (2021). Inhibition of Cell Growth by Anoikis in Various Human Cancer Cell Lines Treated with an Extract of Smilax china L. Journal of Life Science, 31(3), 266-279. https://doi.org/10.5352/JLS.2021.31.3.266.
  • Lučić, D., Pavlović, I., Brkljačić, L., Bogdanović, S., Farkaš, V., Cedilak, A., ... & Salopek-Sondi, B. (2023). Antioxidant and antiproliferative activities of kale (Brassica oleracea L. var. acephala DC.) and wild cabbage (Brassica incana Ten.) polyphenolic extracts. Molecules, 28(4), 1840. https://doi.org/10.3390/ molecules28041840.
  • Mondal, J., Panigrahi, A. K., & Khuda-Bukhsh, A. R. (2014). Anticancer potential of Conium maculatum extract against cancer cells in vitro: Drug-DNA interaction and its ability to induce apoptosis through ROS generation. Pharmacognosy magazine, 10(Suppl 3), S524. https://doi.org/10.4103/0973-1296.139792.
  • Ngo, D. H., Nguyen, H. N. M., Nguyen, T. N. H., Nguyen, T. L. T., Ngo, D. N., & Vo, T. S. (2022). Growth inhibitory activity of Brassica oleracea var. alboglabra on human gastric cancer cells. Journal of Reports in Pharmaceutical Sciences, 11(2), 199-203. https://doi.org/10.4103/jrptps.jrptps_119_21.
  • Ozsoy, N., Can, A., Yanardag, R., & Akev N. (2008). Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chemistry, 110, 571-583. https://doi.org/10.1016/j.foodchem.2008.02.037.
  • Potikanond, S., Sookkhee, S., Na Takuathung, M., Mungkornasawakul, P., Wikan, N., Smith, D. R., & Nimlamool, W. (2017). Kaempferia parviflora extract exhibits anti-cancer activity against HeLa cervical cancer cells. Frontiers in Pharmacology, 8, 630. https://doi.org/10.3389/fphar.2017.00630.
  • Qureshi, A. A., & Barabde, G. (2018). Optimization of extraction process and investigation of Antioxidant Activity, DNA Protection Potential and Antimicrobial activities of Trachyspermum ammi seed extract. IOSR Journal of Applied Chemistry, 11(11), 45-49. http://dx.doi.org/10.9790/5736-1111014549.
  • Raul, S. C., Beatriz, H. C., Joseoziel, L. G., & Francenia, S. S. N. (2017). Phenolic Compounds in Genus Smilax (Sarsaparilla). In M. Soto-Hernandez, M. Palma-Tenango, & M.D.R. Garcia-Mateos, (Eds.), Phenolic Compounds- Natural Sources, Importance and Applications, (pp. 233-260). http://dx.doi.org/10.5772/66896.
  • Rawat, A., & Reddy, A. V. B. (2022). Recent advances on anticancer activity of coumarin derivatives. European Journal of Medicinal Chemistry Reports, 5, 100038. https://doi.org/10.1016/j.ejmcr.2022.100038.
  • Sarialtin, S. Y., Polat, D. C., & Yalcin, C. O. (2023). Cytotoxic and antioxidant activities and phytochemical analysis of Smilax excelsa L. and Aegopodium podagraria L. Food Bioscience, 52, 102359. https://doi.org/10.1016/j.fbio.2023.102359.
  • Shailasree, S., Sampathkumara, K. K., Niranjana, S. R., & Prakash, H. S. (2014). Bioactive potential of medicinal plants from western ghats region, India. Journal of Herbs, Spices & Medicinal Plants, 20(3), 221-234. https://doi.org/10.1080/10496475.2013.860070.
  • Shariatzadeh, M., Karami, A., Moghadam, A., Lotfi, M., Maggi, F., & Ebrahimie, E. (2022). The essential oil from Oliveria decumbens Vent. (Apiaceae) as inhibitor of breast cancer cell (MCF-7) growth. Pharmaceuticals, 16(1), 59. https://doi.org/10.3390/ph16010059.
  • Tang, Z., Qin, Y., Wang, Y., Lin, W., Wang, Q., Shen, N., ... & Ding, C. (2022). The endophytic fungus Penicillium oxalicum isolated from Ligusticum chuanxiong Hort possesses DNA damage-protecting potential and increases stress resistance properties in Caenorhabditis elegans. Frontiers in Pharmacology, 13, 983716. https://doi.org/10.3389/fphar.2022.983716.
  • Topdaş, E. F., Demirbaş, M., Şengül, M., & Şat, İ. G. (2021). Farklı Kurutma Tekniklerinin Smilax excelsa Genç Sürgünlerinin Antioksidan Aktivitesi ve Bazı Fizikokimyasal Özellikleri Üzerine Etkisi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 52(3), 314-324. https://doi.org/10.17097/ataunizfd.816887.
  • Topkara, E. F., Kaba, B., Saygin, H., Ugurtay, E. T., & Pashazadeh, H. (2024). Determination of the Phytochemical, Antioxidant, and Antimicrobial Properties of Smilax excelsa L. Extracted with Different Solvents. Sinop Üniversitesi Fen Bilimleri Dergisi, 9(2), 516-533. https://doi.org/10.33484/sinopfbd.1587996.
  • Venkateswaran, S., Manivannan, H. P., Francis, A. P., Veeraraghavan, V. P., Gayathri, R., & Sankaran, K. (2023). Identification of Potential Phytochemical Inhibitors From Conium maculatum Targeting the Epidermal Growth Factor Receptor in Metastatic Colorectal Cancer via Molecular Docking Analysis. Cureus, 15(10). https://doi.org/10.7759/cureus.48000.
  • Vetter, J. (2004). Poison hemlock (Conium maculatum L.). Food and Chemical Toxicology, 42(9), 1373-1382. https://doi.org/10.1016/j.fct.2004.04.009.
  • Wu, L. S., Wang, X, J., Wang, H., Yang, H. W., Jia, A. Q., & Ding, Q. (2010). Cytotoxic polyphenols against breast tumor cell in Smilax china L. Journal of Ethnopharmacology, 130(3), 460-464. https://doi.org/10.1016/ j.jep.2010.05.032.
  • Yeşiltaş, H. (2018). Brassica oleracea var. viridis ve Smilax excelsa bitki özütlerinin Acanthamoeba castellanii trofozoitleri üzerine in vitro amoebisidal aktivitelerinin araştırılması (Tez no 540195). [Yüksek Lisans Tezi, Ordu Üniversitesi Fen Bilimleri Enstitüsü Moleküler Biyoloji ve Genetik Ana Bilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Yi, J. L., Shi, S., Shen, Y. L., Wang, L., Chen, H. Y., Zhu, J., & Ding, Y. (2015). Myricetin and methyl eugenol combination enhances the anticancer activity, cell cycle arrest and apoptosis induction of cis-platin against HeLa cervical cancer cell lines. International journal of clinical and experimental pathology, 8(2), 1116. https://pmc.ncbi.nlm.nih.gov/articles/PMC4396221/.
  • Zhai, K., Mazurakova, A., Koklesova, L., Kubatka, P., & Büsselberg, D. (2021). Flavonoids synergistically enhance the anti-glioblastoma effects of chemotherapeutic drugs. Biomolecules, 11(12), 1841. https://doi.org/10.3390/ biom11121841.
Toplam 45 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Bitki Hücresi ve Moleküler Biyoloji
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Dr. Bülent Kaynak 0000-0002-9207-5485

Gülizar Aydoğdu 0000-0001-8390-1477

Zeynep Kolören 0000-0001-9708-2716

Onur Kolören 0000-0002-3359-4904

Erken Görünüm Tarihi 10 Haziran 2025
Yayımlanma Tarihi
Gönderilme Tarihi 4 Mart 2025
Kabul Tarihi 14 Mayıs 2025
Yayımlandığı Sayı Yıl 2025Cilt: 28 Sayı: 4

Kaynak Göster

APA Kaynak, D. B., Aydoğdu, G., Kolören, Z., Kolören, O. (2025). Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 945-954. https://doi.org/10.18016/ksutarimdoga.vi.1651205
 Kapak Resmi İndir

21082



2022-JIF = 0.500

2022-JCI = 0.170

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