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Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.)

Yıl 2025, Cilt: 28 Sayı: 5, 1241 - 1258

Özden Salman , Nuh Boyraz

https://doi.org/10.18016/ksutarimdoga.vi.1611970

Öz

This study aims to investigate the biocontrol potential of antagonistic and plant growth-promoting rhizobacteria (PGPR) isolated from plant roots and soils against the crown rot pathogen Rhizoctonia solani AG 2-2 in sugar beet (Beta vulgaris L.) tubers. A total of 219 soil samples were collected from 20 provinces to obtain putative bacterial PGPR isolates that could potentially be used as biological control agents (BCAs). A total of 752 bacterial isolates were obtained, and there in vitro antifungal activity against R. solani AG-2.2 was tested in double culture experiments. The result was that 117 PGPR bacterial isolates exhibited antibiotic properties that inhibited mycelial growth of the fungal pathogen. These isolates were also characterized for their antagonistic and plant growth-promoting properties, including phosphorus solubility, hydrogen cyanide (HCN) activity, siderophore production, ACC deaminase activity, and indole-3-acetic acid (IAA) production. The 12 best PGPR isolates were then subjected to an in vivo pot test using a weighted scoring system. The isolate Bacillus subtilis 119.2 was identified as the most effective isolate that completely inhibited the outbreak of the disease caused by R. solani AG-2.2 in sugar beet. Considering the results of the in vitro and in vivo studies, the B. subtilis 119.2 isolate showed its potential as an environmentally friendly biological control agent against R. solani in sugar beet. Overall, B. subtilis 119.2 is a PGPR isolate with potential for use as a biofertilizer or biofungicide.

Anahtar Kelimeler

Rhizoctonia Bacillus PGPR Antagonist Plant Growth Promoting

Destekleyen Kurum

The project has been supported by the Selçuk University Faculty Member Placement Program with the project number 2019-ÖYP-011 for its execution.

Proje Numarası

2019-ÖYP-011

Kaynakça

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Şeker Pancarında (Beta vulgaris L.) Taç Çürüklüğü Hastalık Etmeni Rhizoctonia solani AG 2-2’ye Karşı Kökbakterilerinin İzolasyonu, Tanılanması ve Biyokontrol Etkinliklerinin Belirlenmesi

Yıl 2025, Cilt: 28 Sayı: 5, 1241 - 1258

Özden Salman , Nuh Boyraz

https://doi.org/10.18016/ksutarimdoga.vi.1611970

Öz

Bu çalışmanın amacı, şeker pancarı (Beta vulgaris L.) yumrularında sorun taç çürüklüğü hastalık etmeni Rhizoctonia solani AG 2-2'ye karşı bitki kök ve topraklardan izole edilmiş potansiyel antagonistik (BCAs) ve bitki gelişimini teşvik eden kökbakterilerinin (PGPR) hastalık etmenine karşı biyokontrol potansiyelini araştırmaktır. Potansiyel biyolojik kontrol etmeni bakteri izolatlarını elde etmek için 20 ilden toplam 219 toprak örneği toplanmıştır. Bu örneklerden 752 bakteri izolatı elde edilmiş ve bu bakterilerin, R. solani AG-2.2'ye karşı in vitro antifungal etkinlikleri, ikili kültür denemeleri ile test edilmiştir. Sonuçta, 117 bakteri izolatının misel gelişimini engelleyen antibiyosis özelliği gösterdiği belirlenmiştir. Bu izolatların antibiyosis etkinliklerinin yanısıra, fosfor çözünürlüğü, hidrojen siyanür (HCN) aktivitesi, siderofor üretimi, ACC deaminaz aktivitesi ve indol-3-asetik asit (IAA) üretimi gibi bitki gelişimini teşvik edici özellikleri karakterize edilmiştir. Test edilen PGPR izolatlar arasında, en iyi 12 bakteri, tartılı derecelendirme sistemi kullanılarak in vivo saksı testlerine tabi tutulmuştur. Bacillus subtilis 119.2 izolatı, şeker pancarında R. solani AG-2.2' kaynaklı hastalık çıkışının engellenmesinde %100 oranla en etkili izolat olarak belirlenmiştir. Hem in vitro hem de in vivo çalışma sonuçları göz önünde bulundurulduğunda, B. subtilis 119.2 izolatının şeker pancarı tarımında R. solani'ye karşı çevre dostu biyolojik mücadele etmeni olarak potansiyelinin bulunduğunu göstermiştir. Tüm sonuçlar birlikte değerlendirildiğinde B. subtilis 119.2 biyolojik gübre veya biyolojik fungisit olarak kullanım potansiyeline sahip PGPR izolatıdır.

Anahtar Kelimeler

Rizobakteriler Bacillus PGPR Antagonist Bitki Gelişimini Teşvik Etme

Destekleyen Kurum

Çalışmanın yürütülebilmesi için Selçuk Üniversitesi Öğretim Üyesi Yerleştirme Programı tarafından 2019-ÖYP-011 nolu proje ile destek sağlanmıştır.

Proje Numarası

2019-ÖYP-011

Kaynakça

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  • Molefe, R. R., Amoo, A.E. & Babalola, O.O. (2023). Communication between plant roots and the soil microbiome; involvement in plant growth and development. Symbiosis, 90(3), 231-239. https://link.springer.com/article/10.1007/s13199-023-00941-9
  • Moussa, T. A. (2002). Studies on biological control of sugarbeet pathogen Rhizoctonia solani Kühn. J. Biol. Sci, 2(12), 800-804. DOI: 10.3923/jbs.2002.800.804
  • Muyolo, N. G. L., P. E. & Schmıtthenner, A. F. (1993). Reactions of dry bean, lima bean, and soybean cultivars to Rhizoctonia root and hypocotyl rot and web blight. Plant disease, 77, 234-238. DOI: 10.1094/PD-77-0234
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  • Penrose, D. M. & Glick, B.R. (2003). Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiologia plantarum, 118(1), 10-15. https://doi.org/10.1034/j.1399-3054.2003.00086.x
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  • Rodrı guez, H. & Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology advances, 17(4-5), 319-339. https://doi.org/10.1016/S0734-9750(99)00014-2
  • Salman, Ö. & Boyraz, N. (2023). Determination of Disease Severity of Rhizoctonia solani Kühn (Telemorph: Thanatephorus cucumeris ((Frank) Donk) Isolates from Bean, Sugar Beet and Potato Planting Areas in Konya. Selcuk Journal of Agriculture and Food Sciences, 37(1), 119-132. DOI: 10.15316/SJAFS.2023.013
  • Salman, Ö. (2023). Bazı Bitkilerde Rhizoctonia spp.’lerinin Biyolojik Mücadele İmkanlarının Araştırılması (797011). [Selçuk Üniversitesi, Fen Bilimleri Enstitüsü Bitki Koruma Ana Bilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Schmid, M., Iversen, C., Gontia, I., Stephan, R., Hofmann, A., Hartmann, A., Jha, B., Eberl, L., Riedel, K. & Lehner, A. (2009). Evidence for a plant associated natural habitat for Cronobacter spp. Research in Microbiology, 160, 8, 608-614. https://doi.org/10.1016/j.resmic.2009.08.013
  • Schmiedeknecht, G., Bochow, H. & Junge, H. (1998). Use of Bacillus subtilis as biocontrol agent. II. Biological control of potato diseases/Anwendung von Bacillus subtilis als Mittel für den biologischen Pflanzenschutz. II. Biologische Bekämpfung von Kartoffelkrankheiten, Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz/Journal of Plant Diseases and Protection, 376-386. https://www.jstor.org/stable/43215255
  • Scholten, O. E., Panella, L. W., De Bock, T. S. & Lange, W. (2001). A greenhouse test for screening sugar beet (Beta vulgaris) for resistance to Rhizoctonia solani, European Journal of Plant Pathology, 107(2), 161-166. https://doi.org/10.1023/A:1011208903344
  • Schwyn, B. & Neilands, J. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical biochemistry, 160(1), 47-56. https://doi.org/10.1016/0003-2697(87)90612-9
  • Sehrawat, A., Sindhu, S. S. & Glick, B. R. (2022). Hydrogen cyanide production by soil bacteria: Biological control of pests and promotion of plant growth in sustainable agriculture. Pedosphere, 32(1), 15-38. https://doi.org/10.1016/S1002-0160(21)60058-9
  • Selva Kumar, S., Ram Krishna Rao, M., Deepak Kumar, R., Panwar, S. & Prasad, C. (2013). Biocontrol by plant growth promoting rhizobacteria against black scurf and stem canker disease of potato caused by Rhizoctonia solani. Archives of phytopathology and plant protection, 46(4), 487-502. https://doi.org/10.1080/03235408.2012.745054
  • Sendi, Y., Pfeiffer, T., Koch, E., Mhadhbi, H. & Mrabet, M. (2020). Potential of common bean (Phaseolus vulgaris L.) root microbiome in the biocontrol of root rot disease and traits of performance. Journal of Plant Diseases and Protection, 127(4), 453-462. https://doi.org/10.1007/s41348-020-00338-6
  • Soylu, E. M., Soylu, S., Kara, M., & Kurt, Ş. (2020). Determinations of in vitro antagonistic effects of microbiomes isolated from vermicompost against major plant fungal disease agents of vegetables. KSU Tarım ve Doğa Dergisi 23(1), 7-18. https://doi.org/10.18016/ksutarimdoga.vi.601936
  • Soylu, S., Kara, M., Uysal, A., Kurt, Ş., & Soylu, E. M. (2021). Determination of antagonistic potential of endophytic bacteria isolated from lettuce against lettuce white mould disease caused by Sclerotinia sclerotiorum. Zemdirbyste-Agriculture, 108(3), 303–312. https://doi.org/10.13080/z-a.2021.108.039
  • Strausbaugh, C. A., Eujayl, I. A., Panella, L. W. & Hanson, L. E. (2011). Virulence, distribution and diversity of Rhizoctonia solani from sugar beet in Idaho and Oregon. Canadian Journal of Plant Pathology, 33(2), 210-226. https://doi.org/10.1080/07060661.2011.558523
  • Sundara, B., Natarajan, V. & Hari, K. (2002). Influence of phosphorus solubilizing bacteria on the changes in soil available phosphorus and sugarcane and sugar yields. Field Crops Research, 77(1), 43-49. https://doi.org/10.1016/S0378-4290(02)00048-5
  • Şahin, K. (2022). Kayseri ilinde şeker pancarı kota uygulamalarının şeker pancarı üretimine etkileri. Journal of the Institute of Science and Technology,12(3), 1801-1807. https://doi.org/10.21597/jist.1030385
  • Şahiner, A. (2020). Kırşehir Ekolojik koşullarında bazı şeker pancarı (Beta vulgaris L.) çeşitlerinin verim ve kalite özelliklerinin belirlenmesi (615138). [Yüksek Lisans Tezi, Kırşehir Ahi Evran Üniversitesi Fen Bilimleri Enstitüsü, Tarla Bitkileri Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
  • Tamura K. & Nei M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10:512-526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
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  • Tariq, M., Yasmin, S. & Hafeez, F. Y. (2010). Biological control of potato black scurf by rhizosphere associated bacteria. Brazilian Journal of Microbiology, 41, 439-451. https://doi.org/10.1590/S1517-83822010000200026
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  • Tsror, L. (2010). Biology, epidemiology and management of Rhizoctonia solani on potato. Journal of Phytopathology, 158(10), 649-658. https://doi.org/10.1111/j.1439-0434.2010.01671.x
  • Turan, M., Ekinci, M., Yildirim, E., Güneş, A., Karagöz, K., Kotan, R. & Dursun, A. (2014). Plant growth-promoting rhizobacteria improved growth, nutrient, and hormone content of cabbage (Brassica oleracea) seedlings. Turkish Journal of Agriculture and Forestry, 38(3), 327-333. https://doi.org/10.3906/tar-1308-62
  • Vacheron, J., Desbrosses, G., Bouffaud, M.L., Touraine, B., Moenne-Loccoz, Muller, D., Legendre, L., Wisniewski-Dye, F., Prigent-Combaret, C. (2013). Plant growth-promoting rhizobacteria and root system functioning. Frontiers in plant science, 4, 356
  • Wagi, S. & Ahmed, A. (2019). Bacillus spp.: potent microfactories of bacterial IAA. PeerJ, 7, e7258.
  • Wang, Y., Pei, Y., Wang, X., Dai, X., & Zhu, M. (2024). Antimicrobial metabolites produced by the plant growth-promoting rhizobacteria (PGPR): Bacillus and Pseudomonas. Advanced Agrochem. https://doi.org/10.1016/j.aac.2024.07.007
  • Webb, K.M., Harveson, R.M. & West, M.S. (2015). Evaluation of Rhizoctonia zeae as a potential biological control option for fungal root diseases of sugar beet. Annals of Applied Biology, 167(1), 75-89. https://doi.org/10.1111/aab.12210
  • Weisburg, W.G., Barns, S.M., Pelletier, D. A. & Lane, D.J. (1991). 16 S ribosomal DNA amplification for phylogenetic study. J. Bacteriol 173(2):697-703. https://doi.org/10.1128/jb.173.2.697-703.1991
  • Widawati, S. (2020). Isolation of Indole Acetic Acid (IAA) producing Bacillus siamensis from peat and optimization of the culture conditions for maximum IAA production. IOP Conference Series: Earth and Environmental Science, 012025. DOI 10.1088/1755-1315/572/1/012025
  • Zahir, Z. A., Ghani, U., Naveed, M., Nadeem, S. M. & Asghar, H. N. (2009). Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for improving growth and yield of wheat (Triticum aestivum L.) under salt-stressed conditions. Archives of microbiology, 191(5), 415-424. https://doi.org/10.1007/s00203-009-0466-y
  • Zhao, C., Li, Y., Wu, S., P., Han, C. & Wu, X. (2019). Anastomosis group and pathogenicity of Rhizoctonia spp. associated with seedling damping-off of sugar beet in China. European journal of plant pathology, 153, 869-878. https://doi.org/10.1007/s10658-018-1603-8
Toplam 97 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Patolojisi, Fitopatoloji
Bölüm ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar

Özden Salman 0000-0002-7871-4105

Nuh Boyraz

Proje Numarası 2019-ÖYP-011
Erken Görünüm Tarihi 25 Temmuz 2025
Yayımlanma Tarihi
Gönderilme Tarihi 2 Ocak 2025
Kabul Tarihi 24 Haziran 2025
Yayımlandığı Sayı Yıl 2025Cilt: 28 Sayı: 5

Kaynak Göster

APA Salman, Ö., & Boyraz, N. (2025). Isolation, Identification and Determination of Biocontrol Activities of Rhizobacteria against Cown Rot Disease Agent Rhizoctonia solani AG 2-2 in Sugar Beet (Beta vulgaris L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(5), 1241-1258. https://doi.org/10.18016/ksutarimdoga.vi.1611970
 Kapak Resmi İndir

21082



2022-JIF = 0.500

2022-JCI = 0.170

Uluslararası Hakemli Dergi (International Peer Reviewed Journal)

       Dergimiz, herhangi bir başvuru veya yayımlama ücreti almamaktadır. (Free submission and publication)

      Yılda 6 sayı yayınlanır. (Published 6 times a year)


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