Yaşam Süresi ve Üreme Arasındaki Dengede Fitokimyasal Modülasyon: Cistus creticus Örneği

Mehmet Fidan

doi:10.18016/ksutarimdoga.vi.1666340

TR EN

Yaşam Süresi ve Üreme Arasındaki Dengede Fitokimyasal Modülasyon: Cistus creticus Örneği

Öz

Bu çalışma, Cistus creticus L. bitkisi ekstraktının Drosophila melanogaster Meigen’de yaşam süresi ve yumurta verimine etkilerini incelemiştir. Polifenol ve terpenoid açısından zengin ekstraktın, maksimum/ortalama ömür, yumurta verimi, antioksidan enzim aktiviteleri süperoksit dismutaz (SOD) katalaz (CAT), glutatyon (GSH) ve foxo gen ekspresyonu üzerindeki etkileri araştırılmıştır. Oregon-R suşu, kontrollü koşullarda yetiştirilmiş; standart besine 0, 1, 10, 50, 100 mg/mL oranlarında C. creticus ekstraktı eklenmiştir. Her grupta 50 erkek ve 50 dişi sinek kullanılmış; yaşam süresi Kaplan-Meier ve Log-rank testiyle, diğer veriler tek yönlü ANOVA ile analiz edilmiştir. Kontrol grubunda ortalama ömür erkeklerde 62,5±3,1, dişilerde 58,2±2,9 gün; maksimum ömür 67 ve 63 gün olarak ölçülmüştür. 1 mg/mL dozda ortalama ömür erkeklerde %12 (70,0±3,4 gün), dişilerde %10 (64,0±3,0 gün) artmış; maksimum ömür 75 ve 69 güne çıkmış; yumurta verimi 48,5±3,0’dan 55,8±3,3’e yükselmiş; SOD, CAT, GSH ve foxo ekspresyonu erkeklerde 1,8±0,2, dişilerde 1,6±0,2 kat artmıştır (p<0,01). 100 mg/mL dozda ise ortalama ömür erkeklerde %28 (45,0±2,2 gün), dişilerde %33 (39,0±2,0 gün) azalmış; maksimum ömür 49 ve 43 güne düşmüş; yumurta verimi %38 gerileyerek 30,0±2,3 olmuş; antioksidan aktiviteler ve foxo ekspresyonu baskılanmıştır (p<0,001). Yaşam süresi ve yumurta verimi arasında negatif korelasyon (r=-0,58, p<0,05) bulunmuş, bu da üreme-ömür dengesi hipotezini desteklemiştir. Düşük dozlar ömür ve üremeyi artırmış, yüksek dozlar ise toksisiteyle her ikisini baskılamıştır. Gelecek çalışmalarda, C. creticus'un yaşlanma süreçlerini etkileyen aktif bileşenlerinin izolasyonu ve foxo sinyal yolağı üzerindeki etki mekanizmalarının aydınlatılması önerilmektedir.

Anahtar Kelimeler

Enzymatic Regulation in the Balance Between Lifespan and Reproduction: The Case of Cistus criticus

Abstract

This study examined the effects of Cistus creticus L. plant extract on lifespan and egg production in Drosophila melanogaster Meigen. The effects of the extract, rich in polyphenols and terpenoids, on maximum/average lifespan, egg yield, antioxidant enzyme activities; superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and foxo gene expression were investigated. The Oregon-R strain was raised under controlled conditions; C. creticus extract was added to the standard diet at concentrations of 0, 1, 10, 50, and 100 mg/mL. Each group included 50 male and 50 female flies; lifespan was analyzed using Kaplan-Meier and Log-rank tests, while other data were evaluated with one-way ANOVA. In the control group, average lifespan was 62.5±3.1 days (males) and 58.2±2.9 days (females), with maximum lifespans of 67 and 63 days. At 1 mg/mL, average lifespan increased by 12% in males (70.0±3.4 days) and 10% in females (64.0±3.0 days); maximum lifespan reached 75 and 69 days; egg yield rose from 48.5±3.0 to 55.8±3.3; and SOD, CAT, GSH, and foxo expression increased by 1.8±0.2-fold (males) and 1.6±0.2-fold (females) (p<0.01). At 100 mg/mL, average lifespan decreased by 28% in males (45.0±2.2 days) and 33% in females (39.0±2.0 days); maximum lifespan dropped to 49 and 43 days; egg yield fell 38% to 30.0±2.3; and antioxidant activities and foxo expression were suppressed (p<0.001). A negative correlation (r=-0.58, p<0.05) between lifespan and egg yield supports the reproduction-lifespan trade-off hypothesis. Low doses enhanced lifespan and reproduction, while high doses suppressed both via toxicity. In future studies, isolation of active compounds of C. creticus that affect aging processes and elucidation of their mechanisms of action on the foxo signaling pathway are recommended.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Bitki Bilimi (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Mehmet Fidan ^*
0000-0001-9016-6730
Türkiye

Erken Görünüm Tarihi

25 Temmuz 2025

Yayımlanma Tarihi

11 Eylül 2025

Gönderilme Tarihi

26 Mart 2025

Kabul Tarihi

14 Haziran 2025

Yayımlandığı Sayı

Yıl 1970 Cilt: 28 Sayı: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1666340

IZ

https://izlik.org/JA87TW58DH

Kaynak Göster

RIS / Bibtex

APA

Fidan, M. (2025). Yaşam Süresi ve Üreme Arasındaki Dengede Fitokimyasal Modülasyon: Cistus creticus Örneği. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(5), 1214-1228. https://doi.org/10.18016/ksutarimdoga.vi.1666340

Yaşam Süresi ve Üreme Arasındaki Dengede Fitokimyasal Modülasyon: Cistus creticus Örneği

Öz

Anahtar Kelimeler

Enzymatic Regulation in the Balance Between Lifespan and Reproduction: The Case of Cistus criticus

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

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