Böceklerde Farklı Muhafaza Koşulları ve Zamanın Mitokondrial DNA Kalitesi Üzerine Etkisi: Bir Diptera (Tephiritidae) Örneği Çalışması
Öz
Bu çalışmada geleneksel öldürme yöntemleri ile elde
edilmiş ve farklı koşullarda muhafaza edilen Tephritidae familyasına ait Euaresta bullans (Wiedemann, 1830)
örneklerinden DNA izole edilerek muhafaza koşulları ve muhafaza sürelerine
bağlı olarak DNA kalitesi ve PZR başarıları araştırılmıştır. Bu anlamda
standart barkod geni olan mitokondriyal sitokrom oksidaz I (COI) gen bölgesi kullanılmıştır. 15 yıllık
örneklerden kuru ortamda (müze materyali) muhafaza edilenlerin yaşı ile PZR
başarısı arasında tam bir ilişki gözlemlenmemiştir. Ancak alkolde (%95’lik etil
alkol) saklanan örneklerde ise DNA kalitesi ve PZR başarısı yüksek bulunmuştur.
Anahtar Kelimeler
Destekleyen Kurum
Gaziantep Üniversitesi BAP Yönetim Birimi
Proje Numarası
FEF.YLT.18.20
Teşekkür
Bu çalışmaya, Gaziantep Üniversitesi, Bilimsel Araştırma Projeleri Biriminin, FEF.YLT.18.20 no’lu proje ile vermiş oldukları destekten dolayı teşekkür ederiz.
Kaynakça
- Barret RDH, Hebert PDN 2005. Identifying spiders through DNA barcodes. Canadian Journal of Zoology, 83: 481-491.
- Catzeflis FM 1991. Animal tissue collections for molecular genetics and systematics. Trends in Ecology and Evolution, 6(5): 168.
- Çalışkan Keçe AF, Özbek Çatal B, Ulusoy MR 2019. A New Invasive Species in Turkey: Dacus ciliatus Loew, 1862 (Diptera: Tephritidae). Turkish Journal of Entomology. 43 (1): 25-30.
- Dean DM, Ballard JWO 2001. Factors affecting mitochondrial DNA quality from museum preserved Drosophila simulans. Entomologia Experimentalis et Applicata 98:279-283.
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5): 294-299
- Goldstein PZ, DeSalle R (2003) Calibrating phylogenetic species formation in a threatened insect using DNA from historical specimens. Mol Ecol 12:1993–1998.
- Hebert PDN, Ratnasingham S, Dewaard JR 2003. Barcoding animal life: cytochrome c oxidase subunit I divergences among closely related species. Proc. Royal Society London B (Suppl.): 596-599.
- Korneyev VA 1999. Phylogenetic relationships among higher groups of Tephritidae. In Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton, xi 944 p (73–113).
- Lodge RJ, Freeland JR 2003, The use of Odonata museum specimens in questions of molecular evolution. Odonatologica, 32:375–380.
- Norrbom AL 1999. A Generic reclassification an pylogeny of the tribe Myopitini (Tephritinae). In Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton. xi 944 p (581 – 627).
- Vink JC, Steven MT, Pierre P, Cheryl HY, Marshal H 2005. The effects of preservatives and temperatures on arachnid DNA. Invertebrate Systematics, 19: 99 -104.
- Watts CPH, Thompson JD, Allen AK, Kemp JS 2007. How useful is DNA extracted from the legs of archived insects for microsatellite-based population genetic analyses. Journal of Insect Conservation, 11:195-198.
- Yaran M, Kütük M, Görmez V, Koyuncu MÖ 2018. A new species and additional record of Terellia Robineau-Desvoidy (Diptera: Tephritidae) from Turkey with a key for the Cerajocera group. Turkish Journal of Zoology. 42: 661-665.
The Effects of Different Storage Conditions and Storage Time on The Quality of The Mitochondrial DNA on Insects: Sample Study is A Fruit Fly (Diptera: Tephiritidae)
Öz
In
this study, DNA was isolated from the samples of Euaresta bullans (Wiedemann, 1830) belonging to the Tephritidae
family which was obtained by traditional killing methods and kept under
different conditions. In this sense, the standard barcode gene mitochondrial
cytochrome oxidase I gene (COI)
was used. The lack of relationship observed between the age of samples that
were kept in dry conditions (museum material) for 15 years and PCR success.
However, the DNA quality and PCR success of the samples stored in alcohol (95%
ethyl alcohol) were found to be high.
Anahtar Kelimeler
Proje Numarası
FEF.YLT.18.20
Kaynakça
- Barret RDH, Hebert PDN 2005. Identifying spiders through DNA barcodes. Canadian Journal of Zoology, 83: 481-491.
- Catzeflis FM 1991. Animal tissue collections for molecular genetics and systematics. Trends in Ecology and Evolution, 6(5): 168.
- Çalışkan Keçe AF, Özbek Çatal B, Ulusoy MR 2019. A New Invasive Species in Turkey: Dacus ciliatus Loew, 1862 (Diptera: Tephritidae). Turkish Journal of Entomology. 43 (1): 25-30.
- Dean DM, Ballard JWO 2001. Factors affecting mitochondrial DNA quality from museum preserved Drosophila simulans. Entomologia Experimentalis et Applicata 98:279-283.
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5): 294-299
- Goldstein PZ, DeSalle R (2003) Calibrating phylogenetic species formation in a threatened insect using DNA from historical specimens. Mol Ecol 12:1993–1998.
- Hebert PDN, Ratnasingham S, Dewaard JR 2003. Barcoding animal life: cytochrome c oxidase subunit I divergences among closely related species. Proc. Royal Society London B (Suppl.): 596-599.
- Korneyev VA 1999. Phylogenetic relationships among higher groups of Tephritidae. In Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton, xi 944 p (73–113).
- Lodge RJ, Freeland JR 2003, The use of Odonata museum specimens in questions of molecular evolution. Odonatologica, 32:375–380.
- Norrbom AL 1999. A Generic reclassification an pylogeny of the tribe Myopitini (Tephritinae). In Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton. xi 944 p (581 – 627).
- Vink JC, Steven MT, Pierre P, Cheryl HY, Marshal H 2005. The effects of preservatives and temperatures on arachnid DNA. Invertebrate Systematics, 19: 99 -104.
- Watts CPH, Thompson JD, Allen AK, Kemp JS 2007. How useful is DNA extracted from the legs of archived insects for microsatellite-based population genetic analyses. Journal of Insect Conservation, 11:195-198.
- Yaran M, Kütük M, Görmez V, Koyuncu MÖ 2018. A new species and additional record of Terellia Robineau-Desvoidy (Diptera: Tephritidae) from Turkey with a key for the Cerajocera group. Turkish Journal of Zoology. 42: 661-665.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Yapısal Biyoloji |
| Bölüm | ARAŞTIRMA MAKALESİ (Research Article) |
| Yazarlar | |
| Proje Numarası | FEF.YLT.18.20 |
| Yayımlanma Tarihi | 31 Aralık 2019 |
| Gönderilme Tarihi | 24 Mayıs 2019 |
| Kabul Tarihi | 23 Ağustos 2019 |
| Yayımlandığı Sayı | Yıl 2019 |
Kaynak Göster
Cited By
Fruit fly (Diptera: Tephritidae) fauna in Tokat province (Türkiye)
International Journal of Chemistry and Technology
https://doi.org/10.32571/ijct.1184571
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