Aromatik Halkalı Bazı Fosfazenlerin Saccharomyces cerevisiae L. Kültüründe Biyokimyasal Aktivitelerinin Belirlenmesi
Öz
Bu çalışmada; yeni sentezlenmiş aromatik halkalı fosfazenlerin S. cerevisiae kültür ortamında toplam protein, glutatyon S-transferaz (GST) ve doymamış yağ asitleri üzerinde ki etkisi belirlendi. Farklı fosfazen molekülleri kullanıldı. Deney grupları kontrol grubu ve fosfazen molekülleri grupları şeklinde düzenlendi. Ardından, S. cerevisiae kültürünün kontrol grubu dışında diğer kültürlere 20 µg fosfazen ilave edildi. 30 °C de 72 saat inkübasyona bırakıldı. İnkübasyon sonunda hücre peletleri ayrıldı ve Tris-ETDA tamponu homojenize edildi. Homojenizattan Glutatyon S-Transferaz (GST) ile total protein düzeyleri belirlendi. Hekzan/izopropanol karışımı ile elde edilen homojenattan da yağ asidi analizi yapıldı. Deney sonuçlarına göre, fosfazen molekülü ilave edilen maya hücrelerinde total protein değerleri ile GST değerlerinde paralel bir şekilde artış gösterirken, bazı gruplarda protein miktarında artış gözlendiği halde GST düzeyinde azalma saptandı. S. cerevisiae yağ asidi bileşimi içinde doymamış yağ asitlerinden oleik asit ile palmitoleik asitlerin yüksek düzeyde olduğu gözlendi. Fosfazenlerin yağ asidi sentezi üzerinde hem azaltıcı yönde hem de artırıcı yönde etkili olduğu gözlendi. Sonuç olarak; yeni sentezlenmiş fosfazen moleküllerinin S. cerevisiae’da biyokimyasal parametreler üzerinde farklı etkilerinin olduğu ortaya konulmuştur. Bu farklı etkinin fosfazenlerin moleküler yapılarında ki farklılığa bağlı olarak açığa çıktığı düşülmektedir.
Anahtar Kelimeler
Fosfazen molekülleri GST Saccharomyces cerevisiae L Total protein Yağ asiti
Destekleyen Kurum
Bitlis Eren Üniversitesi Rektörlüğü Bilimsel Araştırma Projeleri (BAP) birimi
Proje Numarası
BEBAP 2019.008
Teşekkür
Bu çalışma, Bitlis Eren Üniversitesi Rektörlüğü Bilimsel Araştırma Projeleri (BAP) birimi tarafından BEBAP 2019.008 nolu proje numarası ile desteklenmiştir.
Kaynakça
- Atalan M 2016. Diaminospiro Siklotetrafosfazen Türevlerinin DNA Ve Antimikrobiyal Aktivitelerinin İncelenmesi. Yüksek Lisans Tezi, Muş Alparslan Üniversitesi, Fen Bilimleri Enstitüsü, Türkiye.
- Barnett JA, Entian KD 2005. A history of research on yeasts 9: regulation of sugar metabolism, Yeast, 22(11): 835-894.
- Bergman LW 2001. Growth and Maintenance of Yeast. 2001. Methods in Molecular Biology, Vol. 177, TwoHybrid Systems: Methods and Protocols Edited by: P. N. MacDonald © Humana Press Inc., Totowa, NJ
- Cabral Luís MB, José Mata,J 2003. On the Evolution of the Firm Size Distribution: Facts and Theory. American Economic Review, 93 (4): 1075-1090.
- Christie WW 1992. Gas Chromatography and Lipids. Glaskow, The Oil Press.
- Dilsiz N, Çelik S, Yılmaz Ö, Dıgrak M 1997. The effects of selenium, vitamin E and their combination on the composition of fatty acids and proteins in Saccharomyces cerevisiae. Cell Biochemistry and Function, 15: 265-69.
- Dumanoğulları F 2006. Bs (Fenoks) Fosfazen Türevlerinin Sentez ve Yapıları. Yüksek Lisans Tezi, Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Kimya Anabilim Dalı, Ankara.
- Fernandes PMD 2001. How does yeast respond to pressure? Brazilian Journal of Medical and Biological Research, 38: 1239-1245.
- Gudasi KB, Vadavi RS, Sreedhar B, Sairam M, Shelke NB, Mallikarjuna NN, Kulkarni PV, Aminahbavi TM 2007. Synthesis and characterization of some organopolyphosphazenes and their controlled-release characteristics. Designed Monomers and Polymers, 10 (3): 235–251.
- Habig WH, Pabst MJ, Jakoby WB 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249(22):7130-7139.
- Hara A, Radin NS 1978. Lipid extraction of tissues with a low-toxicity solvent. Analytical Biochemistry, 90(1): 420–426.
- Kirecci OA 2017. Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. KSÜ Tarım ve Doğa Dergisi, 20(3): 175-184.
- Koran K, Özen F, Torğut G, Pıhtılı G, Çil E, Görgülü AO, Arslan M 2014. Synthesis, characterization and dielectric properties of phosphazenes containing chalcones. Polyhedron, 79: 213–220.
- Kruckeberg AL, Dickinson JR 2004. Carbon metabolism, The metabolism and molecular physiology of Saccharomyces cerevisiae. CRC, London
- Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ 1951. Protein measurement with the folin-phenol reagent. The Journal of Biochemistry, 193: 265- 277.
- Nedjoud G, Houria B, Rachid R, Amira A, Reda D 2009. Impact of pollution by industrial metallic dust on bio-accumulator organism Helix aspersa. Global Vet, 3: 276-280.
- Nelson DL, Cox MM 2000. Lehninger Principles of Biochemistry. Worth publishers, Third Edition, p. 1152, New York Ntambi, JM 1999. Regulations of Stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. Journal of lipid research, 409:1549-1558.
- Özen F, Koran K, Aslan F, Arslan M 2014. Hekza-(2-Formil–5-Metoksifenoksi) Siklotrifosfazen’in Schiff Bazı Türevlerinin Sentezi ve Karakterizasyonu, European Journal of Science and Technology, 183): 69-75.
- Ribeiro IC, Verıssimo I, Moniz L, Cardoso H, Sousa MJ, Soares AMVM, Lea˜o C 2000. Yeasts as A Model for Assessing The Toxicity of The Fungicides Penconazol, Cymoxanil and Dichlofluanid. Chemosphere, 41: 1637-1642.
- Rimoldi OJ, Finarelli GS, Brenner RR 2001. Effectes of diabetes and insulin on hepatic Delta 6 desaturese gene expression. Biochemical and Biophysical Research Communications, 283(2), 323-326.
- Singh S, Brocker C, Koppaka V, Chen Y, Jackson BC, Matsumoto A, Thompson, DC, Vasiliou V 2013. Aldehyde Dehydrogenases in Cellular Responses to Oxidative/Electrophilic Stres. Free Radical Biology and Medicine, 56: 89-101.
- Tamarit J, Cabiscol E, Ros J 1998. Identification of the major oxidatively damaged proteins in Escherichia coli cells exposed to oxidative stres. Journal of Biological Chemistry, 273: 3027 – 3032.
- Tvrzicka E, Vecka M, Stankova B, Zak A 2002. Analysis of fatty acids in plasma lipoproteins by gas chromatography flame ionisation detection quantitative aspects. Analytica Chimica Acta, 465:337-350.
- Vincent AM, Russell JW, Low P, Feldman EL 2004. Oxidative Stres in the Pathogenesis of Diabetic Neuropathy. Journal of Endocrinology Revision, 25(4): 612-28.
- Voet, D, Voet JG, Wiley J, Pratt CW 1999. Fundamentals of Biochemistry, NY: John Wiley & Sons, Inc New York.
Determination of Biochemical Activities of Aromatic Cyclic Phosphatans in the Culture of Saccharomyces cerevisiae L.
Öz
In this study; the effect of newly synthesized different aromatic cyclic phosphazenes on total protein, glutathione S-transferase (GST) and unsaturated fatty acids in S. cerevisiae culture medium was determined. The experimental groups were organized as control group, and phosphazen molecules groups, then, 20 µg of phosphazen was added to other cultures, except for the control group of S. cerevisiae culture. All treatments were incubated for 72 hours at 30 °C. At the end of incubation, cell pellets were separated and Tris-ETDA buffer was homogenized. Total protein levels were determined from Glutathione S-Transferase (GST) from homogenized. The fatty acid was analyzed from the homogenate obtained with the hexane/ isopropanol mixture. According to the results of the experiment, while the total protein values and GST values increased in parallel in the yeast cells to which the phosphazene molecule was added, a decrease in the GST level was found in some groups although the protein amount was increased. It was observed that high levels of oleic acid and palmitoleic acids were among the unsaturated fatty acids in the S. cerevisiae fatty acid composition. It was observed that phosphazenes had both a decreasing and increasing effect on the fatty acid synthesis compared to the molecular groups. It can be concluded that newly synthesized phosphosene molecules have different effects on biochemical parameters in S. cerevisiae. It was thought that this different effect occurs due to the difference in the molecular structure of the phosphocenes.
Anahtar Kelimeler
Fatty acid GST Phosphazene molecules S. cerevisiae Total protein
Proje Numarası
BEBAP 2019.008
Kaynakça
- Atalan M 2016. Diaminospiro Siklotetrafosfazen Türevlerinin DNA Ve Antimikrobiyal Aktivitelerinin İncelenmesi. Yüksek Lisans Tezi, Muş Alparslan Üniversitesi, Fen Bilimleri Enstitüsü, Türkiye.
- Barnett JA, Entian KD 2005. A history of research on yeasts 9: regulation of sugar metabolism, Yeast, 22(11): 835-894.
- Bergman LW 2001. Growth and Maintenance of Yeast. 2001. Methods in Molecular Biology, Vol. 177, TwoHybrid Systems: Methods and Protocols Edited by: P. N. MacDonald © Humana Press Inc., Totowa, NJ
- Cabral Luís MB, José Mata,J 2003. On the Evolution of the Firm Size Distribution: Facts and Theory. American Economic Review, 93 (4): 1075-1090.
- Christie WW 1992. Gas Chromatography and Lipids. Glaskow, The Oil Press.
- Dilsiz N, Çelik S, Yılmaz Ö, Dıgrak M 1997. The effects of selenium, vitamin E and their combination on the composition of fatty acids and proteins in Saccharomyces cerevisiae. Cell Biochemistry and Function, 15: 265-69.
- Dumanoğulları F 2006. Bs (Fenoks) Fosfazen Türevlerinin Sentez ve Yapıları. Yüksek Lisans Tezi, Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Kimya Anabilim Dalı, Ankara.
- Fernandes PMD 2001. How does yeast respond to pressure? Brazilian Journal of Medical and Biological Research, 38: 1239-1245.
- Gudasi KB, Vadavi RS, Sreedhar B, Sairam M, Shelke NB, Mallikarjuna NN, Kulkarni PV, Aminahbavi TM 2007. Synthesis and characterization of some organopolyphosphazenes and their controlled-release characteristics. Designed Monomers and Polymers, 10 (3): 235–251.
- Habig WH, Pabst MJ, Jakoby WB 1974. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249(22):7130-7139.
- Hara A, Radin NS 1978. Lipid extraction of tissues with a low-toxicity solvent. Analytical Biochemistry, 90(1): 420–426.
- Kirecci OA 2017. Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. KSÜ Tarım ve Doğa Dergisi, 20(3): 175-184.
- Koran K, Özen F, Torğut G, Pıhtılı G, Çil E, Görgülü AO, Arslan M 2014. Synthesis, characterization and dielectric properties of phosphazenes containing chalcones. Polyhedron, 79: 213–220.
- Kruckeberg AL, Dickinson JR 2004. Carbon metabolism, The metabolism and molecular physiology of Saccharomyces cerevisiae. CRC, London
- Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ 1951. Protein measurement with the folin-phenol reagent. The Journal of Biochemistry, 193: 265- 277.
- Nedjoud G, Houria B, Rachid R, Amira A, Reda D 2009. Impact of pollution by industrial metallic dust on bio-accumulator organism Helix aspersa. Global Vet, 3: 276-280.
- Nelson DL, Cox MM 2000. Lehninger Principles of Biochemistry. Worth publishers, Third Edition, p. 1152, New York Ntambi, JM 1999. Regulations of Stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. Journal of lipid research, 409:1549-1558.
- Özen F, Koran K, Aslan F, Arslan M 2014. Hekza-(2-Formil–5-Metoksifenoksi) Siklotrifosfazen’in Schiff Bazı Türevlerinin Sentezi ve Karakterizasyonu, European Journal of Science and Technology, 183): 69-75.
- Ribeiro IC, Verıssimo I, Moniz L, Cardoso H, Sousa MJ, Soares AMVM, Lea˜o C 2000. Yeasts as A Model for Assessing The Toxicity of The Fungicides Penconazol, Cymoxanil and Dichlofluanid. Chemosphere, 41: 1637-1642.
- Rimoldi OJ, Finarelli GS, Brenner RR 2001. Effectes of diabetes and insulin on hepatic Delta 6 desaturese gene expression. Biochemical and Biophysical Research Communications, 283(2), 323-326.
- Singh S, Brocker C, Koppaka V, Chen Y, Jackson BC, Matsumoto A, Thompson, DC, Vasiliou V 2013. Aldehyde Dehydrogenases in Cellular Responses to Oxidative/Electrophilic Stres. Free Radical Biology and Medicine, 56: 89-101.
- Tamarit J, Cabiscol E, Ros J 1998. Identification of the major oxidatively damaged proteins in Escherichia coli cells exposed to oxidative stres. Journal of Biological Chemistry, 273: 3027 – 3032.
- Tvrzicka E, Vecka M, Stankova B, Zak A 2002. Analysis of fatty acids in plasma lipoproteins by gas chromatography flame ionisation detection quantitative aspects. Analytica Chimica Acta, 465:337-350.
- Vincent AM, Russell JW, Low P, Feldman EL 2004. Oxidative Stres in the Pathogenesis of Diabetic Neuropathy. Journal of Endocrinology Revision, 25(4): 612-28.
- Voet, D, Voet JG, Wiley J, Pratt CW 1999. Fundamentals of Biochemistry, NY: John Wiley & Sons, Inc New York.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Yapısal Biyoloji |
| Bölüm | ARAŞTIRMA MAKALESİ (Research Article) |
| Yazarlar | |
| Proje Numarası | BEBAP 2019.008 |
| Yayımlanma Tarihi | 30 Nisan 2022 |
| Gönderilme Tarihi | 5 Nisan 2021 |
| Kabul Tarihi | 27 Mayıs 2021 |
| Yayımlandığı Sayı | Yıl 2022 |
Kaynak Göster
Cited By
Saccharomyces cerevisiae L. Hücre Kültürü Oksidatif Stres Modelinde Bazı Fosfazen Türevlerinin Biyokimyasal Aktiviteleri
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
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