Evaluation of Biofungicides in the Control against Powdery Mildew Disease [Leveillula taurica (Lev.) Arm.] in Pepper
Öz
In this study, Tea Tree Oil, (Timorex Gold), Orange Oil (Prev-Am), Reynoutria spp. extract (Regalia), Bacillus subtilis QST 713 (Serenade SC), Lactobacillus acidophilus, Lactobacillus paracasei (Vitanal) and a reference product Fluopyram+Tebuconazole (Luna Experience) were applied to pepper plants to determine the efficacy against powdery mildew disease caused by Leveillula taurica (Lev.) Arm on pepper. The disease on plants was evaluated based on the 0-5 scale when the disease reached to 20% on untreated plots. Trials were established as a randomized plot design with four replications. The disease severity was assessed using Towsend-Heuberger’s formula and the percentage effect of the applications was calculated using the Abbott formula. The conducted experiments were revealed that the highest % efficacy values of the preparations against powdery mildew were obtained from Timorex Gold (80%), Prev-Am (45%), Regalia (44.7%), Serenade (26.3%) and Vitanal (26.2%), respectively. The efficacy of the reference product, Luna Experience, against powdery mildew in pepper was found 89%. It has been concluded that Timorex Gold preparation performed enough efficacy against Leveillula taurica, and can be safely used and offered against powdery mildew.
Anahtar Kelimeler
Bacillus subtilis QST 713 Lactobacillus spp. Reynoutria spp. extract Orange oil Tea tree oil
Kaynakça
- Abbott WS 1925. A Method of Computing the Effectiveness of an İnsecticide, Journal of Economic Entomology, 18: 265-267.
- Anonymous 2018. https://www.tarimorman.gov.tr/ TAGEM/Belgeler/Sitandard/Sebze %20Hastal% C4%B1klar%C4%B1%20Standart%20%C4%B0la%C3%A7%20Deneme%20Metotlar%C4%B1.pdf
- Aydın MH 2015. Bitki Fungal Hastalıklarıyla Biyolojik Savaşta Trichoderma’lar. Türkiye Tarımsal Araştırmalar Dergisi, 2(2): 135-148.
- Bora T, Özaktan H 1996. Antagonistik Bazı Floresent Pseudomonasların Önemli Kültür Mantarı Hastalıklarına Karşı İn Vitro Etkileri Üzerinde Araştırmalar. Türkiye 5. Yemeklik Mantar Kongresi Bildiri Özetleri, 5-7 Kasım 1996, Yalova, s: 267-273.
- Ben Abdallah D, Frikha-Gargouri O, Tounsi S 2015. Bacillus amyloliquefaciens Strain 32a As A Source Of Lipopeptides For Biocontrol Of Agrobacterium tumefaciens Strains. Journal of Applied Microbiology, 119: 196–207.
- Dagostin S, Schärer HJ, Pertot I, Tamm L 2011. Are There Alternatives to Copper for Controlling Grapevine Downy Mildew İn Organic Viticulture. Crop Protection, 30 (7): 776-788.
- Daranas N, Roselló G, Cabrefiga J, Donati I, Francés J, Badosa E, Spinelli F, Montesinos E, Bonaterra A 2019. Biological Control of Bacterial Plant Diseases with Lactobacillus plantarum Strains Selected for Their Broad‐Spectrum Activity. Annals of Applied Biology, 174(1): 92-105.
- Grosu AI, Sicuia, OA, Dobre A, Voaideş C, Cornea CP 2015. Evaluation of Ssome Bacillus spp. Strains For The Biocontrol of Fusarium graminearum and F. culmorum in Wheat. Agriculture and Agricultural Science Procedia, 6: 559-566.
- Jochum CC, Osborne LE, Yuen. GY 2006. Fusarium Head Blight Biological Control With Lysobacter Enzymogenes Strain C3. Biological Conservation 39: 336-344.
- Hermosa R., Viterbo A., Chet I., Monte E 2012. Plant-Beneficial Effects of Trichoderma and of Its Genes. Microbiology, 158: 17–25.
- Koç E, İşlek C, Üstün AS 2014. Biber (Capsicum annuum L. cv. Kahramanmaras-Acı) Fidelerinde Bakır Sülfatın (CuSO4) Fenilalanin amonyum liyaz (PAL) Enzim Aktivitesi, Fenolik Maddeler ve Toplam Klorofil Üzerine Etkisi. Süleyman Demirel Üniversitesi. Fen Bilimleri Enstitüsü Dergisi, 13(2): 128-131
- Konstantinidou-Doltsinis S, Markelleu E, Kasselaki AM, Fanouraki NM, Koumaki CM, Schmitt A, Liopa-Tsakalidis A, Malathrakis NE 2006. Efficacy of Milana, A Formulated Plant Extract From Reynoutria sachalinensis, Against Powdery Mildew Of Tomato (Leveillula taurica). BioControl, 51:375-392.
- Lipińska L, Klewicki R, Klewicka E, Kołodziejczyk K., Sójka M, Nowak A 2016. Antifungal Activity of Lactobacillus sp. Bacteria İn The Presence Of Xylitol And Galactosyl-Xylitol. BioMed Research International Volume 2016, Article ID 5897486, 8 pages
- Malathrakis NE, Markellou E, Fanouraki MN, Kasselaki AM, Koumaki CM, Schmitt A, Petsikos-Panayotarou N, Konstantinidou-Doltsinis S 2002. Efficacy of Milsana® (VP 1999), A Formulated Plant Extract From Reynoutria sachalinensis Against Powdery Mildew Of Tomato (Leveillula taurica). In: Elad, Y.;
- Moyer C, Peres NA 2008. Evaluation of Biofungicides For Control of Powdery Mildew Of Gerbera Daisy. Proceedings of the Florida State Horticultural Society , 121: 389-394.
- Reuveni M, Neifeld, D, Dayan D, Kotzer Y 2009. A Novel Organic Product Based On Essential Tea Tree Oil for the Control of Fungal Dıseases in Tomato. Acta Horticulture 808: 129-132 DOI:10.17660/ActaHortic. 2009.08.18.
- Schmitt A, Mauch-Mani B 2002. Proceedings of the IOBC-WPRS Study Group on Induced Resistance in Plants Against Insects And Diseases, Wageningen, The Netherlands, 26-28 April 2001. Bulletin-OILB-SROP 25: (6), 83- 88.
- Shao X, Cheng S, Wang H, Yu D, Mungai C 2013. The Possible Mechanism of Antifungal Action of Tea Tree Oil on Botrytis cinerea. Journal of Applied Microbiology, 114(6), 1642-1649.
- Singh R, Maurya S, Upadhyay RS 2012. Antifungal Potential of Trichoderma Species Against Macrophomina phaseolina. Journal of Agricultural Science and Technology, 8(6): 1925-1933.
- Singh UB, Singh S, Malviya D, Karthikeyan N, Imran M, Chaurasia R, Alam M, Singh P, Sarma BK, Rai JP, Damodaran T, Tripathi JK, Kumar S, Sharma AK 2019. Integration of Anti-Penetrant Tricyclazole, Signaling Molecule Salicylic Acid And Root Associated Pseudomonas fluorescens Enhances Suppression of Bipolaris sorokiniana in Bread Wheat (Triticum aestivum L.). Journal of Plant Pathology, 101 (4): 943-954
- Sülü SM, Bozkurt İA, Soylu S 2016. Bacterial Endophytes as Plant Growth Promoting And Biological Control Agents. Ziraat Fakültesi Dergisi, Mustafa Kemal Üniversitesi, 21(1): 103-111.
- Terzi V, Morcia C, Faccioli P, Vale G, Tacconi G, Malnati M 2007. In vitro Antifungal Activity of The Tea Tree (Melaleuca alternifolia) Essential Oil and İts Majör. Letters in Applied Microbiology 44(6):613‐618.
- Townsend GK, Heuberger JW 1943. Methods for Estimating Losses Caused by Diseases in Fungicide Experiments, Plant Disease Report 27: 340-343
- Tsror L, Lebiush MS, Shapira N 2004. Control of Powdery Mildew on Organic Pepper. Management of Plant Diseases and Arthropod Pests by BCAs. IOBC/WPRS Bulletin 27:333–336
- Ünsal B 2010. Organik Domates Yetiştiriciliğinde Alternaria alternata (Fr.) Keissler İle Mücadele Olanakları, Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı Yüksek Lisans Tezi47s, Aydın.
- Walters DR, Fountaine JM 2009. Practical Application of Induced Resistance to Plant Diseases: an Appraisal of Effectiveness Under Field Conditions. Journal of Agricultural Science, 1-13.
- Yıldız M, Erkan S, Delen N 1990. Sera sebze yetiştiriciliğinin hastalıklar açısından durumu. Türkiye 5. Seracılık Sempozyumu, 17-19 Ekim 1990, İzmir, s: 155-164.
- Yücel S 2008. Survey Studies on Fungal Disease Of Covered Vegetable Areas İn Mediterranean Region. Bitki Koruma Bülteni, 0 (0), Retrieved from http://dergipark.gov.tr/bitkorb/issue/3653/48680.
- Zalila-Kolsi I, Mahmoud AB, Ali, H, Sellami S, Nasfi Z, Tounsi S, Jamoussi K 2016. Antagonist Effects of Bacillus spp. Strains Against Fusarium graminearum For Protection of Durum Wheat (Triticum turgidum L. subsp. durum). Microbiological Research, 192: 148-158.
Biberde Külleme [Leveillula taurica (Lev.) Arm.] Hastalığına Karşı Mücadelede Biyofungisitlerin Değerlendirilmesi
Öz
Bu çalışmada Çay Ağacı Yağı (Timorex Gold), Portakal Yağı (Prev-Am), Reynoutria spp. extrakt (Regalia), Bacillus subtilis QST 713 (Serenade SC), Lactobacillus acidophilus, Lactobacillus paracasei (Vitanal) ve ruhsatlı preperat olarak Fluopyram+Tebuconazole (Luna Experience) Leveillula taurica'(Lev.)’nın neden olduğu külleme hastalığına karşı etkinliğini belirlemek için biber bitkilerine uygulanmıştır. Hastalık şiddeti, kontrol parsellerinde en az %20 hastalık ortaya çıktığında 0–5 skalasına göre değerlendirilmiştir. Serada tesadüf blokları deneme deseninde 4 tekerrürlü bir deneme yürütülmüştür.. Sayım sonucu elde edilen skala değerlerine Towsend-Heuberger formülü uygulanarak hastalık oranları (%), Abbott formülüne göre de preperatların etkileri hesaplanmıştır. Leveillula taurica hastalığına karşı kullanılan preperatların etkinlikleri sırasıyla Timorex Gold (%80), Prev-Am (%45), Regalia (%44.7), Serenade (%26.3), Vitanal (%26.2) olarak belirlenmiştir. Ruhsatlı fungisit olarak kullanılan Luna Experience ise %89 oranında etkili bulunmuştur. Sonuç olarak, Timorex Gold’un biberde külleme hastalığı etmeni Leveillula taurica’ya karşı yüksek etki gösterdiği, güvenle kullanılabileceği ve önerilebileceği kanısına varılmıştır.
Anahtar Kelimeler
Bacillus subtilis QST 713 Lactobacillus spp. Portakal yağı Reynoutria spp. Ektrakt Çay ağacı yağı
Kaynakça
- Abbott WS 1925. A Method of Computing the Effectiveness of an İnsecticide, Journal of Economic Entomology, 18: 265-267.
- Anonymous 2018. https://www.tarimorman.gov.tr/ TAGEM/Belgeler/Sitandard/Sebze %20Hastal% C4%B1klar%C4%B1%20Standart%20%C4%B0la%C3%A7%20Deneme%20Metotlar%C4%B1.pdf
- Aydın MH 2015. Bitki Fungal Hastalıklarıyla Biyolojik Savaşta Trichoderma’lar. Türkiye Tarımsal Araştırmalar Dergisi, 2(2): 135-148.
- Bora T, Özaktan H 1996. Antagonistik Bazı Floresent Pseudomonasların Önemli Kültür Mantarı Hastalıklarına Karşı İn Vitro Etkileri Üzerinde Araştırmalar. Türkiye 5. Yemeklik Mantar Kongresi Bildiri Özetleri, 5-7 Kasım 1996, Yalova, s: 267-273.
- Ben Abdallah D, Frikha-Gargouri O, Tounsi S 2015. Bacillus amyloliquefaciens Strain 32a As A Source Of Lipopeptides For Biocontrol Of Agrobacterium tumefaciens Strains. Journal of Applied Microbiology, 119: 196–207.
- Dagostin S, Schärer HJ, Pertot I, Tamm L 2011. Are There Alternatives to Copper for Controlling Grapevine Downy Mildew İn Organic Viticulture. Crop Protection, 30 (7): 776-788.
- Daranas N, Roselló G, Cabrefiga J, Donati I, Francés J, Badosa E, Spinelli F, Montesinos E, Bonaterra A 2019. Biological Control of Bacterial Plant Diseases with Lactobacillus plantarum Strains Selected for Their Broad‐Spectrum Activity. Annals of Applied Biology, 174(1): 92-105.
- Grosu AI, Sicuia, OA, Dobre A, Voaideş C, Cornea CP 2015. Evaluation of Ssome Bacillus spp. Strains For The Biocontrol of Fusarium graminearum and F. culmorum in Wheat. Agriculture and Agricultural Science Procedia, 6: 559-566.
- Jochum CC, Osborne LE, Yuen. GY 2006. Fusarium Head Blight Biological Control With Lysobacter Enzymogenes Strain C3. Biological Conservation 39: 336-344.
- Hermosa R., Viterbo A., Chet I., Monte E 2012. Plant-Beneficial Effects of Trichoderma and of Its Genes. Microbiology, 158: 17–25.
- Koç E, İşlek C, Üstün AS 2014. Biber (Capsicum annuum L. cv. Kahramanmaras-Acı) Fidelerinde Bakır Sülfatın (CuSO4) Fenilalanin amonyum liyaz (PAL) Enzim Aktivitesi, Fenolik Maddeler ve Toplam Klorofil Üzerine Etkisi. Süleyman Demirel Üniversitesi. Fen Bilimleri Enstitüsü Dergisi, 13(2): 128-131
- Konstantinidou-Doltsinis S, Markelleu E, Kasselaki AM, Fanouraki NM, Koumaki CM, Schmitt A, Liopa-Tsakalidis A, Malathrakis NE 2006. Efficacy of Milana, A Formulated Plant Extract From Reynoutria sachalinensis, Against Powdery Mildew Of Tomato (Leveillula taurica). BioControl, 51:375-392.
- Lipińska L, Klewicki R, Klewicka E, Kołodziejczyk K., Sójka M, Nowak A 2016. Antifungal Activity of Lactobacillus sp. Bacteria İn The Presence Of Xylitol And Galactosyl-Xylitol. BioMed Research International Volume 2016, Article ID 5897486, 8 pages
- Malathrakis NE, Markellou E, Fanouraki MN, Kasselaki AM, Koumaki CM, Schmitt A, Petsikos-Panayotarou N, Konstantinidou-Doltsinis S 2002. Efficacy of Milsana® (VP 1999), A Formulated Plant Extract From Reynoutria sachalinensis Against Powdery Mildew Of Tomato (Leveillula taurica). In: Elad, Y.;
- Moyer C, Peres NA 2008. Evaluation of Biofungicides For Control of Powdery Mildew Of Gerbera Daisy. Proceedings of the Florida State Horticultural Society , 121: 389-394.
- Reuveni M, Neifeld, D, Dayan D, Kotzer Y 2009. A Novel Organic Product Based On Essential Tea Tree Oil for the Control of Fungal Dıseases in Tomato. Acta Horticulture 808: 129-132 DOI:10.17660/ActaHortic. 2009.08.18.
- Schmitt A, Mauch-Mani B 2002. Proceedings of the IOBC-WPRS Study Group on Induced Resistance in Plants Against Insects And Diseases, Wageningen, The Netherlands, 26-28 April 2001. Bulletin-OILB-SROP 25: (6), 83- 88.
- Shao X, Cheng S, Wang H, Yu D, Mungai C 2013. The Possible Mechanism of Antifungal Action of Tea Tree Oil on Botrytis cinerea. Journal of Applied Microbiology, 114(6), 1642-1649.
- Singh R, Maurya S, Upadhyay RS 2012. Antifungal Potential of Trichoderma Species Against Macrophomina phaseolina. Journal of Agricultural Science and Technology, 8(6): 1925-1933.
- Singh UB, Singh S, Malviya D, Karthikeyan N, Imran M, Chaurasia R, Alam M, Singh P, Sarma BK, Rai JP, Damodaran T, Tripathi JK, Kumar S, Sharma AK 2019. Integration of Anti-Penetrant Tricyclazole, Signaling Molecule Salicylic Acid And Root Associated Pseudomonas fluorescens Enhances Suppression of Bipolaris sorokiniana in Bread Wheat (Triticum aestivum L.). Journal of Plant Pathology, 101 (4): 943-954
- Sülü SM, Bozkurt İA, Soylu S 2016. Bacterial Endophytes as Plant Growth Promoting And Biological Control Agents. Ziraat Fakültesi Dergisi, Mustafa Kemal Üniversitesi, 21(1): 103-111.
- Terzi V, Morcia C, Faccioli P, Vale G, Tacconi G, Malnati M 2007. In vitro Antifungal Activity of The Tea Tree (Melaleuca alternifolia) Essential Oil and İts Majör. Letters in Applied Microbiology 44(6):613‐618.
- Townsend GK, Heuberger JW 1943. Methods for Estimating Losses Caused by Diseases in Fungicide Experiments, Plant Disease Report 27: 340-343
- Tsror L, Lebiush MS, Shapira N 2004. Control of Powdery Mildew on Organic Pepper. Management of Plant Diseases and Arthropod Pests by BCAs. IOBC/WPRS Bulletin 27:333–336
- Ünsal B 2010. Organik Domates Yetiştiriciliğinde Alternaria alternata (Fr.) Keissler İle Mücadele Olanakları, Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü, Bitki Koruma Anabilim Dalı Yüksek Lisans Tezi47s, Aydın.
- Walters DR, Fountaine JM 2009. Practical Application of Induced Resistance to Plant Diseases: an Appraisal of Effectiveness Under Field Conditions. Journal of Agricultural Science, 1-13.
- Yıldız M, Erkan S, Delen N 1990. Sera sebze yetiştiriciliğinin hastalıklar açısından durumu. Türkiye 5. Seracılık Sempozyumu, 17-19 Ekim 1990, İzmir, s: 155-164.
- Yücel S 2008. Survey Studies on Fungal Disease Of Covered Vegetable Areas İn Mediterranean Region. Bitki Koruma Bülteni, 0 (0), Retrieved from http://dergipark.gov.tr/bitkorb/issue/3653/48680.
- Zalila-Kolsi I, Mahmoud AB, Ali, H, Sellami S, Nasfi Z, Tounsi S, Jamoussi K 2016. Antagonist Effects of Bacillus spp. Strains Against Fusarium graminearum For Protection of Durum Wheat (Triticum turgidum L. subsp. durum). Microbiological Research, 192: 148-158.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Ziraat, Veterinerlik ve Gıda Bilimleri |
| Bölüm | ARAŞTIRMA MAKALESİ (Research Article) |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Nisan 2022 |
| Gönderilme Tarihi | 6 Nisan 2021 |
| Kabul Tarihi | 20 Mayıs 2021 |
| Yayımlandığı Sayı | Yıl 2022Cilt: 25 Sayı: 2 |
Cited By
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