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Investigation of the Antidiabetic, Antitrosinase, Antioxidant, and Cytotoxic Effects of Different Extracts Prepared from Opuntia ficus-indica (L.) Mill. plant

Year 2024, Volume: 27 Issue: Ek Sayı 1 (Suppl 1), 185 - 193, 25.12.2024

Leyla Paşayeva , Sena Kıcalı , Ayşe Kübra Karaboğa Arslan

https://doi.org/10.18016/ksutarimdoga.vi.1463666

Abstract

The Flora of Türkiye contains many plant species and varieties due to its different climate and ecological conditions. It has great economic potential in terms of cultivated and natural medicinal plants. In this study, the effects of extracts obtained from the fruit part of the O. ficus-indica plant by different extraction methods were examined on antidiabetic, antityrosinase, antioxidant, and cell viability. For this purpose, after drying the fruits, the extracts were prepared with %70 methanol by maceration and ultrasound-assisted extraction methods. Then the α-amylase, α-glucosidase, and tyrosinase inhibitory effects as well as antioxidant capacities with DPPH and ABTS methods and cell viability of extracts were determined on RL95-2 and A549 cancer cells. As a result, it was shown that, the inhibitory effect of the extract prepared by ultrasound-assisted extraction method on α-amylase (IC50=395.123±3.477 µg ml-1) and tyrosinase enzyme (IC50=551.633±1.159 µg ml-1 and ABTS radical scavenging activity (0.522±0.041 µMTrolox/gextract) was found to be higher than the other extract. It was also determined that the total phenolic and flavonoid content of the same extract was higher ((181.189±4,576 mgGAE/extract and 125.635±1.946 mgCA/extract). The effects of the extracts on α-glucosidase and tyrosinase enzymes and cell viability were found to be moderate. In conclusion, further studies are needed to understand which compounds were related to α-amylase inhibitory effect and antioxidant activity and mechanisms of action.

Keywords

Dikenli incir Hypoglycemic effect Cell viability

Project Number

Bu çalışma Erciyes Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi tarafından TLO-2023-12968 kodlu proje ile desteklenmiştir.

References

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  • Belviranlı, B., Al‐Juhaimi, F., Özcan, M. M., Ghafoor, K., Babiker, E. E., & Alsawmahi, O. N. (2019). Effect of location on some physico‐chemical properties of prickly pear (Opuntia ficus‐indica L.) fruit and seeds. Journal of Food Processing and Preservation, 43(3), e13896.
  • Berraaouan, A., Abderrahim, Z., Hassane, M., Abdelkhaleq, L., Mohammed, A., & Mohamed, B. (2015). Evaluation of protective effect of cactus pear seed oil (Opuntia ficus-indica L. Mill.) against alloxan-induced diabetes in mice. Asian Pac J Trop Med, 8(7), 532-537. https://doi.org/10.1016/ j.apjtm.2015.06.013
  • Bourhia, M., Elmandaoui, H., Ullah, R., Ibenmoussa, S., & Shahat, A. A. (2020). Physicochemical evaluation of the fruit pulp of spp growing in the Mediterranean area under hard climate conditions. Open Chemistry, 18(1), 565-575. https://doi.org/ 10.1515/chem-2020-0097
  • Butterweck, V., Semlin, L., Feistel, B., Pischel, I., Bauer, K., & Verspohl, E. J. (2011). Comparative evaluation of two different Opuntia ficus-indica extracts for blood sugar lowering effects in rats. Phytother Res, 25(3), 370-375. https://doi.org/ 10.1002/ptr.3271
  • Cano, M. P., Gomez-Maqueo, A., Fernandez-Lopez, R., Welti-Chanes, J., & Garcia-Cayuela, T. (2019). Impact of high hydrostatic pressure and thermal treatment on the stability and bioaccessibility of carotenoid and carotenoid esters in astringent persimmon (Diospyros kaki Thunb, var. Rojo Brillante). Food Res Int, 123, 538-549. https://doi.org/10.1016/j.foodres.2019.05.017
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  • Dumanoğlu, Z., Güzel, Ü., & Çakır, A. (2020). Doğu Akdeniz bölgesinde yetişen dikenli incir (Opuntia ficus-indica L.) tohumlarının bazı fiziksel özelliklerinin belirlenmesi üzerine bir araştırma. Türk Doğa ve Fen Dergisi, 9(Özel Sayı), 28-33.
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  • Fatullayev, H., Pasayeva, L., Celik, I., Ince, U., & Tugay, O. (2023). Phytochemical composition, in vitro antimicrobial, antioxidant, enzyme ınhibition activities, in silico molecular docking dynamics simulations of Centaurea lycaonica: a computational and experimental approach. ACS omega, 8(25), 22854-22865. https://doi.org/10.1021/ acsomega.3c01819
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  • Giraldo-Silva, L., Ferreira, B., Rosa, E., & Dias, A. C. P. (2023). Opuntia ficus-indica fruit: a systematic review of its phytochemicals and pharmacological activities. Plants (Basel), 12(3), 543. https://doi.org/10.3390/plants12030543
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  • Gouws, C. A., D'Cunha, N. M., Georgousopoulou, E. N., Mellor, D. D., & Naumovski, N. (2019). The effect of different drying techniques on phytochemical content and in vitro antioxidant properties of Australian-grown prickly pears (Opuntia ficus indica). Journal of Food Processing and Preservation, 43(3), e13900. https://doi.org/ 10.1111/jfpp.13900
  • Güven, L., & Gülçin, İ. (2024). Determination of metabolic profiling by LC-MS/MS, evaluation of antioxidant activities, and enzyme inhibition effects of Helichrysum plicatum subsp. pseudopliacatum. KSÜ J. Agric Nat, 27(3), 501-514.
  • Hwang, S. H., Kang, I. J., & Lim, S. S. (2017). Antidiabetic effect of fresh nopal (Opuntia ficus-indica) in low-dose streptozotocin-induced diabetic rats fed a high-fat diet. Evid Based Complement Alternat Med, 2017, 4380721. https://doi.org/ 10.1155/2017/4380721
  • Ishtiaque, S., Naz, S., Siddiqi, R., Umer Abdullah, S., Khan, K., Ahmed, J., & Badaruddin, M. (2014). Antioxidant activities and total phenolics contents from extracts of Terminalia catappa, Carrisa carandas, and Opuntia ficus indica fruits. Recent Innovations in Chemical Engineering (Formerly Recent Patents on Chemical Engineering), 7(2), 106-112. https://doi.org/10.2174/2211334707666 150306 230804
  • Kaewnarin, K., Suwannarach, N., Kumla, J., & Lumyong, S. (2016). Phenolic profile of various wild edible mushroom extracts from Thailand and their antioxidant properties, anti-tyrosinase and hyperglycaemic inhibitory activities. Journal of Functional Foods, 27, 352-364. https://doi.org/ 10.1016/j.jff.2016.09.008
  • Karaboğa-Arslan, A. K., & Yerer, M. B. (2018). α-Chaconine and α-Solanine inhibit RL95-2 endometrium cancer cell proliferation by reducing expression of Akt (Ser473) and ERα (Ser167). Nutrients, 10(6), 672. https://doi.org/10.3390/ nu10060672
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Opuntia ficus-indica (L.) Mill. Bitkisinden Hazırlanan Farklı Ekstrelerin Antidiyabetik, Antitrozinaz, Antioksidan ve Sitotoksik Etkisinin Araştırılması

Year 2024, Volume: 27 Issue: Ek Sayı 1 (Suppl 1), 185 - 193, 25.12.2024

Leyla Paşayeva , Sena Kıcalı , Ayşe Kübra Karaboğa Arslan

https://doi.org/10.18016/ksutarimdoga.vi.1463666

Abstract

Türkiye farklı iklim ve ekolojik koşullara sahip olması nedeniyle floranın çok sayıda bitki türü ve çeşidi içermesi bakımından doğadan toplanan ve kültürü yapılan tıbbi bitkiler açısından büyük bir ekonomik potansiyele sahiptir. Çalışmada Opuntia ficus-indica bitkisinin meyve kısmından farklı ekstraksiyon yöntemleriyle elde edilen ekstrelerin antidiyabetik, antitirozinaz, antioksidan ve hücre canlılığı üzerine etkileri incelenmiştir. Bu amaçla meyve kısmı kurutulduktan sonra %70’lik metanol ile maserasyon ve ses dalgaları-destekli sıvı ekstraksiyonu yöntemleriyle hazırlanan ekstrelerin α-amilaz, α-glikozidaz, tirozinaz inhibitör etkileri yanında antioksidan kapasiteleri DPPH ve ABTS yöntemleri ve hücre canlılığı üzerine etkileri ise RL95-2 ve A549 kanser hücrelerinde tayin edilmiştir. Sonuç olarak ekstrelerden ses dalgaları-destekli sıvı ekstraksiyonu yöntemiyle hazırlanan ekstrenin α-amilaz (IC50=395.123±3.477 µg ml-1) ve tirozinaz enzimi üzerinde inhibisyon etkisinin (IC50=551.633±1.159 µg ml-1), ABTS radikal süpürücü aktivitesinin (0.522±0.041 µMTrolox/gekstre) diğer ekstreden daha yüksek olduğu görülmüştür. Ayrıca aynı ekstresinin toplam fenolik medde ve flavonoit miktarının da daha yüksek olduğu belirlenmiştir ((181.189±4.576 mgGAE/gekstre ve 125.635±1.946 mgCA /gekstre ). Ekstrelerin α-glikozidaz, antitirozinaz ve hücre canlılığı üzerine etkileri orta düzeyde bulunmuştur. Bu çalışmanın sonuçları ile özellikle ses dalgaları-destekli sıvı ekstraksiyonu yöntemiyle hazırlanan ekstrenin α-amilaz inhibitör etki ve antioksidan etkisinin hangi bileşiklerden kaynaklandığını ve etki mekanizmalarını belirlemek için daha ileri çalışmalara ihtiyaç duyulmaktadır.

Keywords

Dikenli incir Hipoglisemik etki Hücre canlılığı

Supporting Institution

Erciyes Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi

Project Number

Bu çalışma Erciyes Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi tarafından TLO-2023-12968 kodlu proje ile desteklenmiştir.

References

  • Arıtuluk, Z. (2012). Halk arasında diyabete karşı kullanılan bitkiler Türkiye-II. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, 32(2), 179-208.
  • Association, A. D. (2010). Diagnosis and classification of diabetes mellitus. Diabetes care, 33(Supplement_1), S62-S69. https://doi.org/ 10.2337/dc10-S062
  • Atiya, A., Majrashi, T. A., Begum, M. Y., Abdul Qadir, S. F., Alqahtani, A. S., Ali Alosman, A. S., Alahmari, A. A., Mesfer Al Aldabsh, A. N., Alshahrani, A. T., & Alshahrani, R. R. M. (2023). Influence of solvent selection and extraction methods on the determination of polyphenols, antioxidant, lipoxygenase and tyrosinase inhibition activities of Opuntia ficus-indica fruits peel and pulp collected from the Kingdom of Saudi Arabia (KSA). Nat Prod Res, 37(3), 514-521. https://doi.org/10.1080/ 14786419.2021.1983571
  • Belviranlı, B., Al‐Juhaimi, F., Özcan, M. M., Ghafoor, K., Babiker, E. E., & Alsawmahi, O. N. (2019). Effect of location on some physico‐chemical properties of prickly pear (Opuntia ficus‐indica L.) fruit and seeds. Journal of Food Processing and Preservation, 43(3), e13896.
  • Berraaouan, A., Abderrahim, Z., Hassane, M., Abdelkhaleq, L., Mohammed, A., & Mohamed, B. (2015). Evaluation of protective effect of cactus pear seed oil (Opuntia ficus-indica L. Mill.) against alloxan-induced diabetes in mice. Asian Pac J Trop Med, 8(7), 532-537. https://doi.org/10.1016/ j.apjtm.2015.06.013
  • Bourhia, M., Elmandaoui, H., Ullah, R., Ibenmoussa, S., & Shahat, A. A. (2020). Physicochemical evaluation of the fruit pulp of spp growing in the Mediterranean area under hard climate conditions. Open Chemistry, 18(1), 565-575. https://doi.org/ 10.1515/chem-2020-0097
  • Butterweck, V., Semlin, L., Feistel, B., Pischel, I., Bauer, K., & Verspohl, E. J. (2011). Comparative evaluation of two different Opuntia ficus-indica extracts for blood sugar lowering effects in rats. Phytother Res, 25(3), 370-375. https://doi.org/ 10.1002/ptr.3271
  • Cano, M. P., Gomez-Maqueo, A., Fernandez-Lopez, R., Welti-Chanes, J., & Garcia-Cayuela, T. (2019). Impact of high hydrostatic pressure and thermal treatment on the stability and bioaccessibility of carotenoid and carotenoid esters in astringent persimmon (Diospyros kaki Thunb, var. Rojo Brillante). Food Res Int, 123, 538-549. https://doi.org/10.1016/j.foodres.2019.05.017
  • Chavez-Santoscoy, R. A., Gutierrez-Uribe, J. A., & Serna-Saldivar, S. O. (2009). Phenolic composition, antioxidant capacity and in vitro cancer cell cytotoxicity of nine prickly pear (Opuntia spp.) juices. Plant Foods Hum Nutr, 64(2), 146-152. https://doi.org/10.1007/s11130-009-0117-0
  • Dumanoğlu, Z., Güzel, Ü., & Çakır, A. (2020). Doğu Akdeniz bölgesinde yetişen dikenli incir (Opuntia ficus-indica L.) tohumlarının bazı fiziksel özelliklerinin belirlenmesi üzerine bir araştırma. Türk Doğa ve Fen Dergisi, 9(Özel Sayı), 28-33.
  • El-Beltagi, H. S., Mohamed, H. I., Elmelegy, A. A., Eldesoky, S. E., & Safwat, G. (2019). Phytochemical screening, antimicrobial, antiaxidant, anticancer activities and nutritional values of cactus (Opuntia ficus indica) pulp and peel. Fresenius Environmental Bulletin, 28(2a), 1545-1562. <Go to ISI>://WOS:000461270800063
  • El Mannoubi, I. (2021). Effect of extraction solvent on phenolic composition, antioxidant and antibacterial activities of skin and pulp of Tunisian red and yellow–orange Opuntia ficus indica fruits. Journal of Food Measurement and Characterization, 15(1), 643-651.
  • Fatullayev, H., Pasayeva, L., Celik, I., Ince, U., & Tugay, O. (2023). Phytochemical composition, in vitro antimicrobial, antioxidant, enzyme ınhibition activities, in silico molecular docking dynamics simulations of Centaurea lycaonica: a computational and experimental approach. ACS omega, 8(25), 22854-22865. https://doi.org/10.1021/ acsomega.3c01819
  • Ferrari, M., Fornasiero, M. C., & Isetta, A. M. (1990). MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods, 131(2), 165-172. https://doi.org/10.1016/0022-1759(90)90187-z
  • Floegel, A., Kim, D. O., Chung, S. J., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of food composition and analysis, 24(7), 1043-1048. https://doi.org/10.1016/j.jfca.2011.01.008
  • Frati, A. C., Jimenez, E., & Ariza, C. R. (1990). Hypoglycemic effect of Opuntia-ficus-indica in non-insulin-dependent Diabetes-Mellitus patients. Phytotherapy Research, 4(5), 195-197. https://doi.org/DOI 10.1002/ptr.2650040507
  • Giraldo-Silva, L., Ferreira, B., Rosa, E., & Dias, A. C. P. (2023). Opuntia ficus-indica fruit: a systematic review of its phytochemicals and pharmacological activities. Plants (Basel), 12(3), 543. https://doi.org/10.3390/plants12030543
  • Gómez‐Maqueo, A., Ortega‐Hernández, É., Serrano‐Sandoval, S. N., Jacobo‐Velázquez, D. A., García‐Cayuela, T., Cano, M. P., & Welti‐Chanes, J. (2020). Addressing key features involved in bioactive extractability of vigor prickly pears submitted to high hydrostatic pressurization. Journal of food process engineering, 43(1), e13202. https://doi.org/ 10.1111/jfpe.13202
  • Gouws, C. A., D'Cunha, N. M., Georgousopoulou, E. N., Mellor, D. D., & Naumovski, N. (2019). The effect of different drying techniques on phytochemical content and in vitro antioxidant properties of Australian-grown prickly pears (Opuntia ficus indica). Journal of Food Processing and Preservation, 43(3), e13900. https://doi.org/ 10.1111/jfpp.13900
  • Güven, L., & Gülçin, İ. (2024). Determination of metabolic profiling by LC-MS/MS, evaluation of antioxidant activities, and enzyme inhibition effects of Helichrysum plicatum subsp. pseudopliacatum. KSÜ J. Agric Nat, 27(3), 501-514.
  • Hwang, S. H., Kang, I. J., & Lim, S. S. (2017). Antidiabetic effect of fresh nopal (Opuntia ficus-indica) in low-dose streptozotocin-induced diabetic rats fed a high-fat diet. Evid Based Complement Alternat Med, 2017, 4380721. https://doi.org/ 10.1155/2017/4380721
  • Ishtiaque, S., Naz, S., Siddiqi, R., Umer Abdullah, S., Khan, K., Ahmed, J., & Badaruddin, M. (2014). Antioxidant activities and total phenolics contents from extracts of Terminalia catappa, Carrisa carandas, and Opuntia ficus indica fruits. Recent Innovations in Chemical Engineering (Formerly Recent Patents on Chemical Engineering), 7(2), 106-112. https://doi.org/10.2174/2211334707666 150306 230804
  • Kaewnarin, K., Suwannarach, N., Kumla, J., & Lumyong, S. (2016). Phenolic profile of various wild edible mushroom extracts from Thailand and their antioxidant properties, anti-tyrosinase and hyperglycaemic inhibitory activities. Journal of Functional Foods, 27, 352-364. https://doi.org/ 10.1016/j.jff.2016.09.008
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There are 40 citations in total.

Details

Primary Language Turkish
Subjects Pharmaceutical Botany
Journal Section RESEARCH ARTICLE
Authors

Leyla Paşayeva 0000-0003-3860-7222

Sena Kıcalı 0009-0008-6179-6387

Ayşe Kübra Karaboğa Arslan 0000-0002-4689-0657

Project Number Bu çalışma Erciyes Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi tarafından TLO-2023-12968 kodlu proje ile desteklenmiştir.
Early Pub Date September 15, 2024
Publication Date December 25, 2024
Submission Date April 2, 2024
Acceptance Date August 15, 2024
Published in Issue Year 2024 Volume: 27 Issue: Ek Sayı 1 (Suppl 1)

Cite

APA Paşayeva, L., Kıcalı, S., & Karaboğa Arslan, A. K. (2024). Opuntia ficus-indica (L.) Mill. Bitkisinden Hazırlanan Farklı Ekstrelerin Antidiyabetik, Antitrozinaz, Antioksidan ve Sitotoksik Etkisinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1), 185-193. https://doi.org/10.18016/ksutarimdoga.vi.1463666
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