Morphological, Physiological and Biochemical Responses of Safflower (Carthamus tinctorius L.) Exposed to Salinity Stress

Bedri Keleş; Filiz Akbaş

doi:10.18016/ksutarimdoga.vi.1008284

EN TR

Morphological, Physiological and Biochemical Responses of Safflower (Carthamus tinctorius L.) Exposed to Salinity Stress

Abstract

The morphological, physiological (biomass, water content-WC) and biochemical (proline, membrane damage-malondialdehyde-MDA, H2O2 content) responses of safflower to NaCI salt stress in different concentrations (0, 50, 75, 150, and 300 mM) were investigated for the first time in in vitro conditions in this study. At the end of the 3-week period, it was determined that NaCl had a negative effect on germination percentages. The percentage of germination was 100% in the control group, while it decreased to 30% in 150 mM NaCl and 5% in 300 mM. In general, morphological development of seedlings was significantly slowed down and seedling growth was not observed at 300 mM concentration. It was determined that the WC, fresh weights, shoot and root length decreased in all NaCl concentrations but there was no significant decrease in dry weights. MDA, proline and H2O2 contents increased in safflower seedlings in parallel with the intensity of salt treatments. While the highest MDA and proline content was found in 150 mM NaCl treatment, the highest H2O2 content was found in 75 mM NaCl treatment. In the light of these data, it has been proven that the Balcı safflower variety is sensitive by showing a negative effect on the applied salt concentrations.

Keywords

Tuz Stresine Maruz Bırakılan Aspir (Carthamus tinctorius L.) Bitkisinin Morfolojik, Fizyolojik ve Biyokimyasal Tepkileri

Öz

Aspir tohumlarının farklı konsantrasyonlarda (0, 50, 75, 150, 300 mM) NaCI stresine karşı morfolojik, fizyolojik (biyokütle, su içeriği-WC) ve biyokimyasal (proline, membran hasarı-malondialdehit-MDA, H2O2 içeriği) tepkileri in vitro koşullarda ilk defa incelenmiştir. Üç haftalık kültür sürenin sonunda NaCl tuz stresinin çimlenme yüzdelerini olumsuz etkilediği belirlenmiştir. Çimlenme yüzdesi kontrol grubunda % 100 iken 150 mM NaCl'de % 30'a, 300 mM'de % 5'e düşmüştür. Genel olarak, fidelerin morfolojik gelişimi önemli ölçüde yavaşlamış ve 300 mM konsantrasyonda ise fide büyümesi gözlenmemiştir. Tüm NaCl konsantrasyonlarında su içeriği (WC), taze ağırlık, sürgün ve kök uzunluğunun azaldığı ancak kuru ağırlıklarda önemli bir azalma olmadığı belirlenmiştir. Aspir fidelerinde tuz uygulamalarının yoğunluğuna paralel olarak MDA, proline ve H2O2 içerikleri de artmıştır. En yüksek MDA ve proline içeriği 150 mM NaCl uygulamasında ve en yüksek H2O2 içeriği 75 mM NaCl uygulamasında bulunmuştur. Bu veriler ışığında, Balcı aspir çeşidinin uygulanan tuz konsantrasyonlarında negatif etki göstererek hassas olduğu kanıtlanmıştır.

Anahtar Kelimeler

Supporting Institution

Batman Üniversitesi

Project Number

BTÜBAP-2017-Yüksek Lisans-9

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Details

Primary Language

English

Subjects

Plant Physiology , Structural Biology

Journal Section

Research Article

Authors

Bedri Keleş
0000-0003-0936-2879
Türkiye

Filiz Akbaş ^*
0000-0003-0623-5524
Türkiye

Early Pub Date

June 14, 2023

Publication Date

December 31, 2023

Submission Date

October 11, 2021

Acceptance Date

October 6, 2022

Published in Issue

Year 2023 Volume: 26 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1008284

IZ

https://izlik.org/JA28YA55RB

Cite

RIS / Bibtex

APA

Keleş, B., & Akbaş, F. (2023). Morphological, Physiological and Biochemical Responses of Safflower (Carthamus tinctorius L.) Exposed to Salinity Stress. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(6), 1246-1252. https://doi.org/10.18016/ksutarimdoga.vi.1008284

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