Determination of Entomopathogenic Nematode Persistency with Surface Spraying and Soil Injection Applications in a Peach Orchard

Çiğdem Şahin; Uğur Gözel

doi:10.18016/ksutarimdoga.vi.1059879

EN TR

Determination of Entomopathogenic Nematode Persistency with Surface Spraying and Soil Injection Applications in a Peach Orchard

Abstract

Entomopathogenic nematodes (EPN) are effective biological control agents against underground and cryptic pests. Persistency and survival of EPN in soil after soil application is important for long term success of management programs. In this study, it was aimed to determine the soil persistency of 4 native EPN species after surface spraying and soil injection applications in a peach orchard. In the study, S. feltiae (96), S. carpocapsae 1133, H. bacteriophora 1144 and S. affine 47 species were applied in 30 l water with 140.000 IJ/tree dose per tree by surface spraying and soil injection methods. EPN were applied to soil with a watering can in surface spraying method and with a pulverizator into 5-15 cm depth in soil injection method. After the monthly application, soil samples were collected and EPN presence was tested with G. mellonella larvae and White traps in the laboratory. The study was conducted for 2 times in 2018 and 2019. At the end of the study, EPN persistency in soil was found to be 90 days in 2018 and 150 days in 2019.

Keywords

Spreyleme ve Toprak Enjeksiyonu Yöntemleri ile Toprağa Uygulanan Entomopatojen Nematodların Şeftali Bahçesinde Kalıcılığının Belirlenmesi

Öz

Entomopatojen nematodlar (EPN’ler), toprak ve korunaklı habitatlarda yaşayan zararlılara karşı etkili biyolojik mücadele etmenleridir. EPN’lerin toprağa uygulanmasından sonra topraktaki kalıcılıkları ve yaşamlarını sürdürebilmeleri mücadele çalışmalarında uzun süreli başarı için önemlidir. Bu çalışmada 4 yerel EPN türünün spreyleme sulama ve toprak enjeksiyonu yöntemleri ile şeftali bahçesinde uygulanmasından sonra topraktaki kalıcılık sürelerinin belirlenmesi amaçlanmıştır. Çalışmada, Steinernema feltiae 96, S. carpocapsae 1133, Heterorhabditis bacteriophora 1144 ve S. affine 47 türleri ağaç başına 30 L su içinde 140.000 IJ/ağaç dozunda spreyleme ve toprak enjeksiyonu yöntemleri ile uygulanmıştır. Spreyleme yönteminde, EPN’ler sulama bidonu ile toprak yüzeyine uygulanırken, toprak enjeksiyonu yönteminde bir pülverizatör ile toprağın 5-15 cm derinliğine uygulanmıştır. Uygulamadan sonra aylık olarak toprak örnekleri alınmış ve laboratuvarda EPN varlığı G. mellonella tuzağı yöntemiyle test edilmiştir. Çalışma 2018 ve 2019 yıllarında 2 tekrarlı olarak gerçekleştirilmiştir. Araştırma sonucunda 2018 yılında EPN’lerin toprakta kalıcılıklarının 90 gün, 2019 yılında ise 150 gün sürdüğü belirlenmiştir.

Anahtar Kelimeler

Supporting Institution

Tübitak TOVAG

Project Number

116O361

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Çiğdem Şahin ^*
0000-0002-2019-6704
Türkiye

Uğur Gözel
0000-0003-1363-1189
Türkiye

Publication Date

December 30, 2022

Submission Date

January 19, 2022

Acceptance Date

March 17, 2022

Published in Issue

Year 2022 Volume: 25 Number: Ek Sayı 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1059879

IZ

https://izlik.org/JA97GK28ZK

Cite

RIS / Bibtex

APA

Şahin, Ç., & Gözel, U. (2022). Determination of Entomopathogenic Nematode Persistency with Surface Spraying and Soil Injection Applications in a Peach Orchard. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 1), 158-163. https://doi.org/10.18016/ksutarimdoga.vi.1059879