Evaluation of the Histopathological Changes Accompanied for the Toxic Effects of Diazinon on the Spleen of Mice

Esra Akat; Ozlem Cakici

doi:10.18016/ksutarimdoga.vi.1096018

EN TR

Evaluation of the Histopathological Changes Accompanied for the Toxic Effects of Diazinon on the Spleen of Mice

Abstract

This study was carried out to determine the histopathological changes caused by diazinon in the spleen of Swiss albino mice. Experimental groups containing low dose (30 mg/kg), medium dose (60 mg/kg) and high dose (120 mg/kg) were exposed to diazinon through oral administration for 30 consecutive days. Separation and hemorrhage in the capsule, congestion, enlarged white pulp, amyloid formation, and karyolysis in some megakaryocytes were determined in the splenic parenchyma of the low dose group. An increase in the number of enlarged white pulps, hemorrhage within splenic parenchyma, accumulation of cells into dilated sinusoids and amyloid formation were examined in the medium dose group. Some cells passing from the splenic parenchyma into dilated sinusoids were also observed. Intensive congestion, necrotic areas within spleen tissue, an increase in the number of karyolytic megakaryocytes, fibrosis and some cells passing from the splenic parenchyma into enlarged sinusoids were prominent histological lesions in the high dose group. These results showed that diazinon caused severe dose-related histopathological damages and had the capacity to disrupt functions of the spleen.

Keywords

Diazinonun Farelerin Dalağı Üzerindeki Toksik Etkilerine Eşlik Eden Histopatolojik Değişikliklerin Değerlendirilmesi

Öz

Bu çalışma, diazinonun İsviçre albino farelerinin dalağında oluşturduğu histopatolojik değişiklikleri belirlemek amacıyla yapıldı. Düşük doz (30 mg/kg), orta doz (60 mg/kg) ve yüksek doz (120 mg/kg) içeren deney grupları, ardışık 30 gün boyunca oral yoldan diazinona maruz bırakıldı. Düşük doz grubunun dalak parankiminde kapsülde ayrılma ve kanama, tıkanıklık, beyaz pulpa büyümesi, amiloid oluşumu ve bazı megakaryositlerde karyoliz tespit edildi. Orta doz grubunda genişlemiş beyaz pulpa sayısında artış, dalak parankiminde kanama, hücrelerin genişlemiş sinüzoidlerde birikmesi ve amiloid oluşumu incelendi. Dalak parankiminden dilate sinüzoidlere geçen bazı hücreler de gözlendi. Yoğun konjesyon, dalak dokusunda nekrotik alanlar, karyolitik megakaryosit sayısında artış, fibrozis ve dalak parankiminden büyümüş sinüzoidlere geçen bazı hücreler yüksek doz grubundaki belirgin histolojik lezyonlardı. Bu sonuçlar diazinonun doza bağlı ciddi histopatolojik hasarlara neden olduğunu ve dalağın fonksiyonlarını bozma kapasitesine sahip olduğunu gösterdi.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Esra Akat
0000-0001-7080-3106
Türkiye

Ozlem Cakici ^*
0000-0003-3589-1440
Türkiye

Early Pub Date

May 27, 2023

Publication Date

October 31, 2023

Submission Date

March 30, 2022

Acceptance Date

March 14, 2023

Published in Issue

Year 2023 Volume: 26 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1096018

IZ

https://izlik.org/JA23KE57XZ

Cite

RIS / Bibtex

APA

Akat, E., & Cakici, O. (2023). Evaluation of the Histopathological Changes Accompanied for the Toxic Effects of Diazinon on the Spleen of Mice. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(5), 1099-1107. https://doi.org/10.18016/ksutarimdoga.vi.1096018

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