Selective Cytotoxicity of Chemical Composition of Spirulina platensis Extract In Endometrial Cells: In Vitro and In Silico Approach

Belkis Atasever Arslan; Kaan Yılancıoğlu; Ahmet Can Timuçin; Zeynep Kalkan; Seda Kuşoğlu Gültekin; Selim Çetiner

doi:10.18016/ksutarimdoga.vi.1110478

TR EN

Endometriyal Hücrelerde Spirulina platensis Ekstresinin Kimyasal Bileşiminin Seçici Sitotoksisitesi: İn Vitro ve İn Siliko Yaklaşım

Öz

Microalgae are considered as an ample source of bioactive compounds. These wide variety of chemicals possess promising potential to translate into drug candidates. Hence, the aim of this study is to determine potential anti-cancer effects of Spirulina platensis microalgae extract against endometrial cancer cells. The selective cytotoxic effect and cytotoxicity index of S. platensis microalgae extract on the Ishikawa endometrial cancer cell line, the determination of DNA fragmentation and the change in gene expression levels of apoptosis-related Bad, Poly-ADP Ribose Polymerase-1 (PARP1), p53, Akt1 and caspase-3 signaling proteins were determined. ECV304 human umbilical vein endothelial cell line was used for a control cell line. Cytotoxic effects of 500 μg mL-1 of S. platensis microalgae extract on Ishikawa and ECV304 cells were determined as 43±4.87 % and 22±1.87 % respectively. In order to delineate specific compounds in the extract, GC-MS analysis was further conducted and seven major compounds revealed to be abundantly present. Since DNA fragmentation induced by S. platensis extract might be related interaction of GC-MS identified compounds with PARP1, an in silico analysis was further implemented. Results indicated the presence of a possible PARP1 inhibitory mechanism contributing to the apoptotic response. In conclusion, a possible link between a specific compound found in S. platensis microalgae extract and the cytotoxicity in endometrial cells was formed through combining in vitro and in silico approaches. The result of these approaches has contributed to the identification of potential anti-cancer compound candidates found in S. platensis microalgae extract.

Anahtar Kelimeler

Selective Cytotoxicity of Chemical Composition of Spirulina platensis Extract In Endometrial Cells: In Vitro and In Silico Approach

Abstract

Microalgae are considered as an ample source of bioactive compounds. These wide variety of chemicals possess promising potential to translate into drug candidates. Hence, the aim of this study is to determine potential anti-cancer effects of Spirulina platensis microalgae extract against endometrial cancer cells. The selective cytotoxic effect and cytotoxicity index of S. platensis microalgae extract on the Ishikawa endometrial cancer cell line, the determination of DNA fragmentation and the change in gene expression levels of apoptosis-related Bad, Poly-ADP Ribose Polymerase-1 (PARP1), p53, Akt1 and caspase-3 signaling proteins were determined. ECV304 human umbilical vein endothelial cell line was used for a control cell line. Cytotoxic effects of 500 μg mL-1 of S. platensis microalgae extract on Ishikawa and ECV304 cells were determined as 43±4.87 % and 22±1.87 % respectively. In order to delineate specific compounds in the extract, GC-MS analysis was further conducted and seven major compounds revealed to be abundantly present. Since DNA fragmentation induced by S. platensis extract might be related interaction of GC-MS identified compounds with PARP1, an in silico analysis was further implemented. Results indicated the presence of a possible PARP1 inhibitory mechanism contributing to the apoptotic response. In conclusion, a possible link between a specific compound found in S. platensis microalgae extract and the cytotoxicity in endometrial cells was formed through combining in vitro and in silico approaches. The result of these approaches has contributed to the identification of potential anti-cancer compound candidates found in S. platensis microalgae extract.

Keywords

Supporting Institution

The Scientific and Technological Research Council of Turkey (TUBITAK) 3501.

Project Number

Project No: 113S251

Thanks

This study was supported by the Scientific and Technological Research Council of Türkiye (TUBITAK) 3501 (Project No: 113S251).

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Belkis Atasever Arslan ^*
0000-0001-5827-8484
Türkiye

Kaan Yılancıoğlu
0000-0001-7105-0898
Türkiye

Ahmet Can Timuçin
0000-0002-9483-3593
Türkiye

Zeynep Kalkan
0000-0002-4495-766X
Türkiye

Seda Kuşoğlu Gültekin
0000-0003-0674-1582
Türkiye

Selim Çetiner
0000-0002-9679-2748
Türkiye

Publication Date

June 30, 2023

Submission Date

May 5, 2022

Acceptance Date

October 12, 2022

Published in Issue

Year 1970 Volume: 26 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1110478

IZ

https://izlik.org/JA53LN63TN

Cite

RIS / Bibtex

APA

Atasever Arslan, B., Yılancıoğlu, K., Timuçin, A. C., Kalkan, Z., Kuşoğlu Gültekin, S., & Çetiner, S. (2023). Selective Cytotoxicity of Chemical Composition of Spirulina platensis Extract In Endometrial Cells: In Vitro and In Silico Approach. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(3), 493-503. https://doi.org/10.18016/ksutarimdoga.vi.1110478