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Endometriyal Hücrelerde Spirulina platensis Ekstresinin Kimyasal Bileşiminin Seçici Sitotoksisitesi: İn Vitro ve İn Siliko Yaklaşım

Cilt: 26 Sayı: 3 30 Haziran 2023

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

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Endometriyal Hücrelerde Spirulina platensis Ekstresinin Kimyasal Bileşiminin Seçici Sitotoksisitesi: İn Vitro ve İn Siliko Yaklaşım

Öz

Microalgae are considered as an ample source of bioactive compounds. These wide variety of chemicals possess promising potential to translate into drug candidates. Hence, the aim of this study is to determine potential anti-cancer effects of Spirulina platensis microalgae extract against endometrial cancer cells. The selective cytotoxic effect and cytotoxicity index of S. platensis microalgae extract on the Ishikawa endometrial cancer cell line, the determination of DNA fragmentation and the change in gene expression levels of apoptosis-related Bad, Poly-ADP Ribose Polymerase-1 (PARP1), p53, Akt1 and caspase-3 signaling proteins were determined. ECV304 human umbilical vein endothelial cell line was used for a control cell line. Cytotoxic effects of 500 μg mL-1 of S. platensis microalgae extract on Ishikawa and ECV304 cells were determined as 43±4.87 % and 22±1.87 % respectively. In order to delineate specific compounds in the extract, GC-MS analysis was further conducted and seven major compounds revealed to be abundantly present. Since DNA fragmentation induced by S. platensis extract might be related interaction of GC-MS identified compounds with PARP1, an in silico analysis was further implemented. Results indicated the presence of a possible PARP1 inhibitory mechanism contributing to the apoptotic response. In conclusion, a possible link between a specific compound found in S. platensis microalgae extract and the cytotoxicity in endometrial cells was formed through combining in vitro and in silico approaches. The result of these approaches has contributed to the identification of potential anti-cancer compound candidates found in S. platensis microalgae extract.

Anahtar Kelimeler

Selective Cytotoxicity of Chemical Composition of Spirulina platensis Extract In Endometrial Cells: In Vitro and In Silico Approach

Abstract

Microalgae are considered as an ample source of bioactive compounds. These wide variety of chemicals possess promising potential to translate into drug candidates. Hence, the aim of this study is to determine potential anti-cancer effects of Spirulina platensis microalgae extract against endometrial cancer cells. The selective cytotoxic effect and cytotoxicity index of S. platensis microalgae extract on the Ishikawa endometrial cancer cell line, the determination of DNA fragmentation and the change in gene expression levels of apoptosis-related Bad, Poly-ADP Ribose Polymerase-1 (PARP1), p53, Akt1 and caspase-3 signaling proteins were determined. ECV304 human umbilical vein endothelial cell line was used for a control cell line. Cytotoxic effects of 500 μg mL-1 of S. platensis microalgae extract on Ishikawa and ECV304 cells were determined as 43±4.87 % and 22±1.87 % respectively. In order to delineate specific compounds in the extract, GC-MS analysis was further conducted and seven major compounds revealed to be abundantly present. Since DNA fragmentation induced by S. platensis extract might be related interaction of GC-MS identified compounds with PARP1, an in silico analysis was further implemented. Results indicated the presence of a possible PARP1 inhibitory mechanism contributing to the apoptotic response. In conclusion, a possible link between a specific compound found in S. platensis microalgae extract and the cytotoxicity in endometrial cells was formed through combining in vitro and in silico approaches. The result of these approaches has contributed to the identification of potential anti-cancer compound candidates found in S. platensis microalgae extract.

Keywords

Destekleyen Kurum

The Scientific and Technological Research Council of Turkey (TUBITAK) 3501.

Proje Numarası

Project No: 113S251

Teşekkür

This study was supported by the Scientific and Technological Research Council of Türkiye (TUBITAK) 3501 (Project No: 113S251).

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Belkis Atasever Arslan ^*
0000-0001-5827-8484
Türkiye

Kaan Yılancıoğlu
0000-0001-7105-0898
Türkiye

Ahmet Can Timuçin
0000-0002-9483-3593
Türkiye

Zeynep Kalkan
0000-0002-4495-766X
Türkiye

Seda Kuşoğlu Gültekin
0000-0003-0674-1582
Türkiye

Selim Çetiner
0000-0002-9679-2748
Türkiye

Yayımlanma Tarihi

30 Haziran 2023

Gönderilme Tarihi

5 Mayıs 2022

Kabul Tarihi

12 Ekim 2022

Yayımlandığı Sayı

Yıl 1970 Cilt: 26 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1110478

IZ

https://izlik.org/JA53LN63TN

Kaynak Göster

APA

Atasever Arslan, B., Yılancıoğlu, K., Timuçin, A. C., Kalkan, Z., Kuşoğlu Gültekin, S., & Çetiner, S. (2023). Selective Cytotoxicity of Chemical Composition of Spirulina platensis Extract In Endometrial Cells: In Vitro and In Silico Approach. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 26(3), 493-503. https://doi.org/10.18016/ksutarimdoga.vi.1110478

Arşiv

Cilt: 26 Sayı: 3 , 30.06.2023

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