Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method

Özden Canlı Taşar; Gani Erhan Taşar

doi:10.18016/ksutarimdoga.vi.1128064

EN TR

Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method

Abstract

Keratinase is an important enzyme used for degradation of the keratinous wastes, especially slaughterhouse and poultry-derived wastes, that cause environmental pollution. In the current study, optimum conditions for keratinase production by Micrococcus luteus Y23-18 strain were investigated using Taguchi DOE L9 orthogonal array. For this purpose, the selected environmental factors were initial pH, incubation temperature and time. The optimal conditions were obtained as pH 9.5, temperature 30˚C and 3 days. The obtained results showed that keratinase activity was enhanced approximately 2.3-folds (34.95 U mL-1) when compared with the unoptimized conditions (15.33 U mL-1). As a result, M. luteus Y23-18 is an effective keratinase producer microorganism and Taguchi design of experiment is a useful tool for optimization.

Keywords

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Özden Canlı Taşar ^*
0000-0002-4313-5373
Türkiye

Gani Erhan Taşar
0000-0002-9217-0706
Türkiye

Early Pub Date

May 27, 2023

Publication Date

October 31, 2023

Submission Date

June 8, 2022

Acceptance Date

November 13, 2022

Published in Issue

Year 2023 Volume: 26 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1128064

IZ

https://izlik.org/JA92BP56BN

Cite

RIS / Bibtex

APA

Canlı Taşar, Ö., & Taşar, G. E. (2023). Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(5), 1027-1033. https://doi.org/10.18016/ksutarimdoga.vi.1128064

AMA

1.Canlı Taşar Ö, Taşar GE. Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method. KSU J. Agric Nat. 2023;26(5):1027-1033. doi:10.18016/ksutarimdoga.vi.1128064

Chicago

Canlı Taşar, Özden, and Gani Erhan Taşar. 2023. “Optimization of Keratinase Enzyme Synthesized by Micrococcus Luteus Using Taguchi DOE Method”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 26 (5): 1027-33. https://doi.org/10.18016/ksutarimdoga.vi.1128064.

EndNote

Canlı Taşar Ö, Taşar GE (October 1, 2023) Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 26 5 1027–1033.

IEEE

[1]Ö. Canlı Taşar and G. E. Taşar, “Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method”, KSU J. Agric Nat., vol. 26, no. 5, pp. 1027–1033, Oct. 2023, doi: 10.18016/ksutarimdoga.vi.1128064.

ISNAD

Canlı Taşar, Özden - Taşar, Gani Erhan. “Optimization of Keratinase Enzyme Synthesized by Micrococcus Luteus Using Taguchi DOE Method”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 26/5 (October 1, 2023): 1027-1033. https://doi.org/10.18016/ksutarimdoga.vi.1128064.

JAMA

1.Canlı Taşar Ö, Taşar GE. Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method. KSU J. Agric Nat. 2023;26:1027–1033.

MLA

Canlı Taşar, Özden, and Gani Erhan Taşar. “Optimization of Keratinase Enzyme Synthesized by Micrococcus Luteus Using Taguchi DOE Method”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 26, no. 5, Oct. 2023, pp. 1027-33, doi:10.18016/ksutarimdoga.vi.1128064.

Vancouver

1.Özden Canlı Taşar, Gani Erhan Taşar. Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method. KSU J. Agric Nat. 2023 Oct. 1;26(5):1027-33. doi:10.18016/ksutarimdoga.vi.1128064

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Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1653239

Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method

Abstract

Keywords

Micrococcus luteus Tarafından Sentezlenen Keratinaz Enziminin Taguchi DOE Yöntemi Kullanılarak Optimizasyonu

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Statistical Optimisation of Streptomyces sp. DZ 06 Keratinase Production by Submerged Fermentation of Chicken Feather Meal

Uncovering High-Yield Ectoine Producers from Extreme Environments: Insights from Tuz Lake