Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method

Özden Canlı Taşar; Gani Erhan Taşar

doi:10.18016/ksutarimdoga.vi.1128064

EN TR

Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method

Abstract

Keratinase is an important enzyme used for degradation of the keratinous wastes, especially slaughterhouse and poultry-derived wastes, that cause environmental pollution. In the current study, optimum conditions for keratinase production by Micrococcus luteus Y23-18 strain were investigated using Taguchi DOE L9 orthogonal array. For this purpose, the selected environmental factors were initial pH, incubation temperature and time. The optimal conditions were obtained as pH 9.5, temperature 30˚C and 3 days. The obtained results showed that keratinase activity was enhanced approximately 2.3-folds (34.95 U mL-1) when compared with the unoptimized conditions (15.33 U mL-1). As a result, M. luteus Y23-18 is an effective keratinase producer microorganism and Taguchi design of experiment is a useful tool for optimization.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Özden Canlı Taşar ^*
0000-0002-4313-5373
Türkiye

Gani Erhan Taşar
0000-0002-9217-0706
Türkiye

Erken Görünüm Tarihi

27 Mayıs 2023

Yayımlanma Tarihi

31 Ekim 2023

Gönderilme Tarihi

8 Haziran 2022

Kabul Tarihi

13 Kasım 2022

Yayımlandığı Sayı

Yıl 2023 Cilt: 26 Sayı: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1128064

IZ

https://izlik.org/JA92BP56BN

Kaynak Göster

RIS / Bibtex

APA

Canlı Taşar, Ö., & Taşar, G. E. (2023). Optimization of Keratinase Enzyme synthesized by Micrococcus luteus using Taguchi DOE Method. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 26(5), 1027-1033. https://doi.org/10.18016/ksutarimdoga.vi.1128064