Anthelmintic Activities of Methanol Extracts of Five Different Geophyte Plant Species

Çiğdem Aydın; Ramazan Mammadov

doi:10.18016/ksutarimdoga.vi.1179200

EN TR

Anthelmintic Activities of Methanol Extracts of Five Different Geophyte Plant Species

Abstract

The purpose of this study was to look into the in vitro anthelmintic activities of methanol extracts of Hyacinthella lineata (Steud. ex Schult. & Schult.f.) Chouard, Ornithogalum umbellatum L., Allium reuterianum Boiss., Cyclamen coum Mill., and Sternbergia clusiana (Ker Gawl.) Ker Gawl. ex Spreng.. The paralysis and death times of the Indian earthworm, Cosmocerca ornata, were used to assess anthelmintic activity. Three different concentrations, (5,10 and 20 mg mL-1) of methanol extracts were used to determine the time required to paralyze and to induce death in the earthworms. Albendazole (15 mg mL-1) was used as reference standard drug. Plant extracts at higher concentrations (20 mg mL-1) produced faster paralytic effects and a shorter time to death. The results revealed that S. clusiana killed all of the test worms within 20 ± 0.6 min of exposure and was 100% effective. Among all five plants extracts tested, S. clusiana was found to be highly effective and significant against C. ornata. The findings will aid in understanding the significance and application of these medicinal plant species in the food industry, traditional medicine, and pharmaceutical applications. This anthelmintic study of the plant extracts revealed that traditional medicine may be as effective as modern medicine in combating pathogenic micro-organisms.

Keywords

Beş Farklı Geofit Bitki Türünden Elde Edilen Metanolik Ekstraktların ın vitro Antihelmint Aktivitesinin Araştırılması

Öz

Bu çalışma Hyacinthella lineata, Ornithogalum umbellatum, Allium reuterianum, Cyclamen coum, Sternbergia clusiana türleri metanol ekstraktlarının in vitro antihelmintik aktivitelerini araştırmak için tasarlanmıştır. Hint toprak solucanı Cosmocerca ornata’nın paraliz ve ölüm zamanının hesaplanması ile antihelmint aktivite değerlendirilmesi yapılmıştır. Paraliz ve ölüme yol açan süreyi belirlemek için üç farklı konsantrasyon (5,10 ve 20 mg mL-1) kullanılmıştır. Referans standart olarak Albendazol (15 mg mL-1) kullanılmıştır. Bitki özütleri yüksek konsantrasyonlarda (20 mg mL-1) daha hızlı paralitik etkiler ve daha kısa zamanda ölüm göstermiştir. Sonuçlar göstermiştir ki, Sternbergia clusiana türüne maruz kalan test solucanlarının tümü 20±0.6 dakika içinde ölüm göstererek %100 etkili olmuştur. Beş bitki türü arasında S. clusiana'nın solucanlara (Cosmocerca ornata) karşı oldukça etkili olduğu ve önemli sonuçlar verdiği bulunmuştur. Sonuçlar, bu tıbbi türlerin gıda endüstrisindeki, geleneksel tıptaki ve farmasötik uygulamalardaki önemini ve kullanımını anlamamıza yardımcı olacaktır. Bitki özleri üzerinde yapılan bu antelmintik çalışma, geleneksel tıbbın patojenik mikroorganizmaların üstesinden gelmek için modern tıp kadar etkili olabileceğini ortaya koydu.

Anahtar Kelimeler

Supporting Institution

PAMUKKALE ÜNİVERSİTESİ BAP Birimi, Doktora Projesinden

Thanks

PAMUKKALE ÜNİVERSİTESİ BAP Birimine katkılarından dolayı teşekkür ederiz

References

Aydin C, Mammadov R 2017. Phenolic Composition, Antioxidant, Antibacterial, Larvacidal Against Culex pipiens and Cytotoxic Activites of Hyacinthella lineata Steudel Extracts. International Journal of Food Properties 20(10): 2276-2285.
Berkov S, Bastida J, Tsvetkova R, Viladomat F, Codina C 2009. Alkaloids from Sternbergia colchiciflora. Z Naturforsch 64: 311-316.
Blessing O, Ezea Omonike O, Edith O 2019. Ajaiyeoba In vitro anthelmintic properties of root extracts of three Musa species Journal Pharmacy & Bioresources 16(2): 145-151.
Bokov DO, Krasikova MK, Sergunova EV, Bobkova NV, Kovaleva TY, Bondar AA, et al. 2020. Pharmacognostic, Phytochemical and Ethnopharmacological Potential of Cyclamen coum Mill. Pharmacogn J 12(1): 204-212.
Chybowski J 1997. Study of the anthelmintic activity of garlic extracts. Herba Polonica 43: 383-387.
Dall'acqua S, Castagliuolo I, Brun P, Ditadi F, Palu G, Innocenti G. 2010. Triterpene Glycosides With in Vitro Anti-Inflammatory Activity From Cyclamen repandum tubers Carbohydrate Research 30: 345- 709.
Davis PH, 1978. Flora of Turkey and East Aegean Islands. Vol. 6. Edinburgh University of Edinburgh Press, Edinburgh: 128-135.
Delazar A, Nazifi E, Movafeghi A, Nahar L, Nazemiyeh H, Moghadam SB, Asnaashari S, Sarker SD 2009. Gc-Ms Analysis Of Ornithogalum procerum. Daru 17(1): 33-36.

Ghannamy U, Kopp B, Kubelka W 1987. Cardenolides From Ornithogalum Boucheanum. Planta Medica 2: 172-178.
Ghosh T, Maity TK, Bose A, Dash GK 2005. Anthelmintic activity of Bacopa monierri Indian. Journal Natural Product 21: 16-19.
Guner A, Ozhatay N, Ekim T, Baser KHC. 2000. Flora of Turkey and the East Aegean Islands, Edinburgh University Press, Edinburgh (in Turkish): 11- 184.
Iqbal Z, Nadeem QK, Khan MN, Akhtar MS, Waraich FN 2001. In vitro anthelmintic activity of Allium sativum, Zingiber officinale, Curcurbita mexicana and Ficus religiosa. International Journal of Agriculture And Biology 3(4): 454-457.
Jaradat NA, Al-Masri M, Hussen F, Zaid AN, Ali I, Tammam A, et al. 2017. Preliminary phytochemical and biological screening of Cyclamen coum a member of palestinian flora. Pharmaceutical Sciences 23(3): 231-7.
Kaya Gİ, Sarıkaya B, Cicek D, Somer NÜ 2010. In vitro Cytotoxic Activity of Sternbergia sicula, S. lutea and Pancratium maritimum Extracts. Hacettepe Univ J of the Faculty of Pharmacy 30(1): 41-48.
Kivcak B, Gözler T. 1993. Alkaloids of Sternbergia sicula. Journal of Ege University Faculty of Pharmacy 1(2): 65-71.
Mali RG, Wadekar RR. 2008. In vitro anthelmintic activity of Baliospermum montanum muell Arg roots. Indian J Pharm Sci 70: 131-33.
Mammadov R, Kara Y, Vaizogullar HE 2011. Study on the Phenolic Content, Antioxidant and Antimicrobial Effects of Sternbergia clusiana. Asian Journal of Chemistry; 23(12): 5280-5284.
Mathew B, Davis AP 1999. Sternbergia, (Eds.), Cites Bulb Checklist, Kew. The Trustees of Royal Botanic Gardens pp. 54-55.
Mathew B, Ozhatay N. 2001.The Cyclamen of Turkey: A Guide to the Species of Cyclamen Growing in Turkey. Cyclamen Society, London,32 p.
Murugamani VL, Raju V, Baskar AR, Manjir S, Sankar G 2012. The New Method Developed for Evaluation of Anthelmintic Activity by HouseflyWorms and Compared with Conventional Earthworm Method. Inter. Scholarly Research Network ISRN Pharmacology Volume Article ID 709860, 6 pp.
Nagaich SS 2000. Studies on the anthelmintic activity of Allium sativum (garlic) oil on common poultry worms Ascaridia galli and Heterakis gallinae. J Parasitol App Anim Biol 9: 47-52.
Najjaa H, Zerria K, Fattouch S, Ammar E, Neffati M 2011. Antioxidant and antimicrobial activities of Allium roseum Lazoul, a wild edible endemic species in North Africa. International Journal of Food Properties 14: 371-380.
Plančić M, Božin B, Kladar N, Rat M, Srđenović B 2014. Phytochemical profile and biological activities of the genus Ornithogalum L. (Hyacinthaceae). Biologia Serbica 36(1-2):1-17.
Sanga S, Maoa S, Laoa A, Chena Z, Ho CT 2003. New steroid saponins from the seeds of Allium tuberosum L. Food Chemistry 83: 499-506.
Singh TU, Kumar D, Tandan SK 2008. Paralytic effect of alcoholic extract of Allium sativum and Piper longum on liver amphistome, Gigantocotyle explanatum. Indian J Pharmacol 40(2): 64-68.
Thooyavan G, Karthikeyan J, Bavani G 2018. Anthelmintic activity of abutilon indicum leaf extract on sheep tapeworm Moniezia expansa. In vitro J of Pharmacognosy and Phytochemistry 7(2): 317-321.
Turan M, Mammadov R 2018. Antioxidant, Antimicrobial, Cytotoxic, Larvicidal and Anthelmintic Activities and Phenolic Contents of Cyclamen alpinum. Pharmacology & Pharmacy 9: 100-116.
Unver N, Kaya Gİ, Oztürk T 2005. Antimicrobial Activity of Sternbergia sicula and Sternbergia lutea. Fitoterapia 76: 226-229.
Vidyadhar S, Saidulu M, Gopal TK, Chamundeeswari D, Rao Umamaheswara, Banji D 2010. In Vıtro Anthelmıntıc Actıvıty of The Whole Plant Of Enıcostemma Lıttorale By Usıng Varıous Extracts. International J of Applied Biology and Pharmaceutical Technology I(3): 1119-1125.

Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Çiğdem Aydın ^*
0000-0003-1934-9766
Türkiye

Ramazan Mammadov
0000-0003-2218-5336
Türkiye

Publication Date

December 30, 2022

Submission Date

September 23, 2022

Acceptance Date

November 6, 2022

Published in Issue

Year 2022 Volume: 25 Number: Ek Sayı 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1179200

IZ

https://izlik.org/JA74BF48FM

Cite

RIS / Bibtex

APA

Aydın, Ç., & Mammadov, R. (2022). Anthelmintic Activities of Methanol Extracts of Five Different Geophyte Plant Species. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 2), 376-381. https://doi.org/10.18016/ksutarimdoga.vi.1179200