Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling

Suzan Sahin Dogan; Aytaç Kocabaş

doi:10.18016/ksutarimdoga.vi.1212062

EN TR

Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling

Abstract

Tuz Lake, a thalassohaline lake with a salt rate of 32%, is a unique habitat for a halophilic microbiome. Culture-dependent and independent methods have been applied to identify prokaryotic microbial diversity in Tuz Lake. However, the key processes and genes involved in biogeochemical cycles in Tuz Lake have not been investigated seasonally. The aim of the study is to investigate seasonal gene profiling in Tuz Lake associated with biogeochemical cycling and thereby reveal more about the ecosystem dynamics of this extreme environment. Therefore, the PICRUSt2 tool was applied to analyze the metabolic function of archaeal and bacterial diversity in Tuz Lake. As a result of metabolic functions based on 16S rDNA amplicon sequencing data, it was observed that methane production by H2 and CO2 by anaerobic archaea in Tuz Lake was the predominant methanogenesis pathway. It was determined that sulfur oxidation was the dominant sulfur metabolism, while the reductive citric acid cycle was the dominant carbon fixation pathway.

Keywords

Biyojeokimyasal Döngüyle İlişkili Tuz Gölü'ndeki Mevsimsel Gen Profili

Abstract

Tuz oranı %32 olan talassohalin bir göl olan Tuz Gölü, halofilik mikrobiyom için önemli bir yaşam alanıdır. Tuz Gölü'ndeki prokaryot mikrobiyal çeşitliliği belirlemek için kültüre bağlı ve bağımsız yöntemler uygulanmıştır. Tuz Gölü'ndeki prokaryot mikrobiyal çeşitliliği belirlemek için kültüre bağımlı ve bağımsız yöntemler uygulanmıştır. Bununla birlikte, Tuz Gölü'ndeki biyojeokimyasal döngülerde yer alan temel süreçler ve genler mevsimsel olarak araştırılmamıştır. Çalışmanın amacı, Tuz Gölü'ndeki biyojeokimyasal döngü ile ilişkili mevsimsel gen profilini araştırmak ve bu ekstremofilik ortamın ekosistem dinamiklerini ortaya çıkarmaktır. Bu nedenle, Tuz Gölü'ndeki arke ve bakteri çeşitliliğinin metabolik işlevlerini analiz etmek için PICRUSt2 aracı kullanılmıştır. 16S rDNA amplikon dizileme verilerine dayanan metabolik fonksiyonlar sonucunda, Tuz Gölü'ndeki anaerobik arkelerin H2 ve CO2 ile metan üretiminin baskın metanojenez yolu olduğu gözlenmiştir. Kükürt oksidasyonu baskın kükürt metabolizması iken indirgeyici sitrik asit döngüsü de baskın karbon fiksasyon yolağı olarak saptanmıştır.

Keywords

Supporting Institution

TUBITAK (Turkish Scientific and Technical Research Council)

Project Number

117z966

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Suzan Sahin Dogan ^*
0000-0002-1554-0080
Türkiye

Aytaç Kocabaş
0000-0001-7622-1932
Türkiye

Early Pub Date

January 21, 2024

Publication Date

April 1, 2024

Submission Date

November 30, 2022

Acceptance Date

June 1, 2023

Published in Issue

Year 2024 Volume: 27 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1212062

IZ

https://izlik.org/JA77GJ29AC

Cite

RIS / Bibtex

APA

Sahin Dogan, S., & Kocabaş, A. (2024). Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(2), 273-284. https://doi.org/10.18016/ksutarimdoga.vi.1212062

AMA

1.Sahin Dogan S, Kocabaş A. Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling. KSU J. Agric Nat. 2024;27(2):273-284. doi:10.18016/ksutarimdoga.vi.1212062

Chicago

Sahin Dogan, Suzan, and Aytaç Kocabaş. 2024. “Seasonal Gene Profiling in Tuz Lake With Regard to Biogeochemical Cycling”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 27 (2): 273-84. https://doi.org/10.18016/ksutarimdoga.vi.1212062.

EndNote

Sahin Dogan S, Kocabaş A (April 1, 2024) Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27 2 273–284.

IEEE

[1]S. Sahin Dogan and A. Kocabaş, “Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling”, KSU J. Agric Nat., vol. 27, no. 2, pp. 273–284, Apr. 2024, doi: 10.18016/ksutarimdoga.vi.1212062.

ISNAD

Sahin Dogan, Suzan - Kocabaş, Aytaç. “Seasonal Gene Profiling in Tuz Lake With Regard to Biogeochemical Cycling”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27/2 (April 1, 2024): 273-284. https://doi.org/10.18016/ksutarimdoga.vi.1212062.

JAMA

1.Sahin Dogan S, Kocabaş A. Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling. KSU J. Agric Nat. 2024;27:273–284.

MLA

Sahin Dogan, Suzan, and Aytaç Kocabaş. “Seasonal Gene Profiling in Tuz Lake With Regard to Biogeochemical Cycling”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 27, no. 2, Apr. 2024, pp. 273-84, doi:10.18016/ksutarimdoga.vi.1212062.

Vancouver

1.Suzan Sahin Dogan, Aytaç Kocabaş. Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling. KSU J. Agric Nat. 2024 Apr. 1;27(2):273-84. doi:10.18016/ksutarimdoga.vi.1212062

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https://doi.org/10.18016/ksutarimdoga.vi.1653239