Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling

Suzan Sahin Dogan; Aytaç Kocabaş

doi:10.18016/ksutarimdoga.vi.1212062

EN TR

Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling

Abstract

Tuz Lake, a thalassohaline lake with a salt rate of 32%, is a unique habitat for a halophilic microbiome. Culture-dependent and independent methods have been applied to identify prokaryotic microbial diversity in Tuz Lake. However, the key processes and genes involved in biogeochemical cycles in Tuz Lake have not been investigated seasonally. The aim of the study is to investigate seasonal gene profiling in Tuz Lake associated with biogeochemical cycling and thereby reveal more about the ecosystem dynamics of this extreme environment. Therefore, the PICRUSt2 tool was applied to analyze the metabolic function of archaeal and bacterial diversity in Tuz Lake. As a result of metabolic functions based on 16S rDNA amplicon sequencing data, it was observed that methane production by H2 and CO2 by anaerobic archaea in Tuz Lake was the predominant methanogenesis pathway. It was determined that sulfur oxidation was the dominant sulfur metabolism, while the reductive citric acid cycle was the dominant carbon fixation pathway.

Keywords

Biyojeokimyasal Döngüyle İlişkili Tuz Gölü'ndeki Mevsimsel Gen Profili

Öz

Tuz oranı %32 olan talassohalin bir göl olan Tuz Gölü, halofilik mikrobiyom için önemli bir yaşam alanıdır. Tuz Gölü'ndeki prokaryot mikrobiyal çeşitliliği belirlemek için kültüre bağlı ve bağımsız yöntemler uygulanmıştır. Tuz Gölü'ndeki prokaryot mikrobiyal çeşitliliği belirlemek için kültüre bağımlı ve bağımsız yöntemler uygulanmıştır. Bununla birlikte, Tuz Gölü'ndeki biyojeokimyasal döngülerde yer alan temel süreçler ve genler mevsimsel olarak araştırılmamıştır. Çalışmanın amacı, Tuz Gölü'ndeki biyojeokimyasal döngü ile ilişkili mevsimsel gen profilini araştırmak ve bu ekstremofilik ortamın ekosistem dinamiklerini ortaya çıkarmaktır. Bu nedenle, Tuz Gölü'ndeki arke ve bakteri çeşitliliğinin metabolik işlevlerini analiz etmek için PICRUSt2 aracı kullanılmıştır. 16S rDNA amplikon dizileme verilerine dayanan metabolik fonksiyonlar sonucunda, Tuz Gölü'ndeki anaerobik arkelerin H2 ve CO2 ile metan üretiminin baskın metanojenez yolu olduğu gözlenmiştir. Kükürt oksidasyonu baskın kükürt metabolizması iken indirgeyici sitrik asit döngüsü de baskın karbon fiksasyon yolağı olarak saptanmıştır.

Anahtar Kelimeler

Destekleyen Kurum

TUBITAK (Turkish Scientific and Technical Research Council)

Proje Numarası

117z966

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Yapısal Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Suzan Sahin Dogan ^*
0000-0002-1554-0080
Türkiye

Aytaç Kocabaş
0000-0001-7622-1932
Türkiye

Erken Görünüm Tarihi

21 Ocak 2024

Yayımlanma Tarihi

1 Nisan 2024

Gönderilme Tarihi

30 Kasım 2022

Kabul Tarihi

1 Haziran 2023

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1212062

IZ

https://izlik.org/JA77GJ29AC

Kaynak Göster

RIS / Bibtex

APA

Sahin Dogan, S., & Kocabaş, A. (2024). Seasonal Gene Profiling in Tuz Lake with Regard to Biogeochemical Cycling. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(2), 273-284. https://doi.org/10.18016/ksutarimdoga.vi.1212062