The Evaluation of psbA-trnH IGS Sequences in The Genus Potentilla L. as Barcoding Region

Aykut Yılmaz

doi:10.18016/ksutarimdoga.vi.1329172

EN TR

The Evaluation of psbA-trnH IGS Sequences in The Genus Potentilla L. as Barcoding Region

Abstract

With more than 400 species, the genus Potentilla has a wide distribution causing morphological variations. Besides the wide distribution, many conditions such as hybridization, introgression, autopolyploidy, and allopolyploidy are observed in the genus. These can lead to misidentifications and taxonomic problems. DNA barcoding studies are important for species identification and solving taxonomic problems. However, preferred DNA sequences may have different effects among plant groups. Therefore, which DNA regions should be preferred is important to obtain more comprehensive results. In this research, psbA-trnH IGS sequences belonging to the Potentilla taxa were examined based on their compatibility and analyzed variable sites, passim-info sites, and nucleotide frequencies (%) for the region relevant by using Molecular Evolutionary Genetics Analysis (MEGA 11). Finally, a Maximum Parsimony (MP) dendrogram was performed to evaluate the Potentilla taxa taxonomically and phylogenetically. As a result of this study, it can be stated that the taxa belonging to the genus Potentilla were well grouped in the dendrogram and the use of psbA-trnH IGS sequences is strongly recommended for further studies.

Keywords

Barkodlama Bölgesi Olarak Potentilla L. Cinsinde psbA-trnH IGS Sekanslarının Değerlendirmesi

Öz

Potentilla cinsi 400’den fazla türle, morfolojik değişimlere neden olan geniş bir yayılışa sahiptir. Geniş yayılışın yanı sıra, cinsteki hibridizasyon, introgression, otopoliploidi ve allopoliploidi gibi birçok durum gözlemlenir. Bunlar, yanlış tanımlamalara ve taksonomik problemlere yol açabilir. DNA barkodlama çalışmaları, tür tanımlama ve taksonomik problemlerin çözümünde önemlidir. Ancak, tercih edilen DNA sekansları, bitki grupları arasında farklı etkilere sahip olabilir. Bu nedenle, hangi DNA bölgelerinin tercih edilmesi gerektiği daha kapsamlı sonuçlar elde etmek için önemlidir. Bu çalışmada, Potentilla taksonlarına ait psbA-trnH IGS sekansları uyumlulukları açısından incelendi ve variable bölgeler, parsim-info bölgeler ve ilgili bölge için nükleotit frekansları (%) Molecular Evolutionary Genetics Analysis (MEGA 11) kullanılarak analiz edildi. Son olarak, Maximum Parsimony (MP) dendrogram taksonomik ve filogenetik olarak Potentilla taksonlarını değerlendirmek için oluşturuldu. Çalışmanın sonucu olarak Potentilla cinsine ait taksonların dendrogramda iyi gruplandığı ve psbA-trnH IGS sekanslarının daha sonraki çalışmalarda kullanımının fayda sağlayacağı söylenebilir.

Anahtar Kelimeler

Thanks

The author would like to thank to NCBI for sequence information used in this study and the authors sharing the sequence informations in NCBI.

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Details

Primary Language

English

Subjects

Plant Biotechnology , Plant Cell and Molecular Biology , Botany (Other)

Journal Section

Research Article

Authors

Aykut Yılmaz ^*
0000-0002-0327-8388
Türkiye

Early Pub Date

April 23, 2024

Publication Date

August 15, 2024

Submission Date

July 18, 2023

Acceptance Date

December 3, 2023

Published in Issue

Year 2024 Volume: 27 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1329172

IZ

https://izlik.org/JA38MM46BK

Cite

RIS / Bibtex

APA

Yılmaz, A. (2024). The Evaluation of psbA-trnH IGS Sequences in The Genus Potentilla L. as Barcoding Region. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(4), 773-781. https://doi.org/10.18016/ksutarimdoga.vi.1329172

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