The Evaluation of psbA-trnH IGS Sequences in The Genus Potentilla L. as Barcoding Region

Aykut Yılmaz

doi:10.18016/ksutarimdoga.vi.1329172

EN TR

The Evaluation of psbA-trnH IGS Sequences in The Genus Potentilla L. as Barcoding Region

Abstract

With more than 400 species, the genus Potentilla has a wide distribution causing morphological variations. Besides the wide distribution, many conditions such as hybridization, introgression, autopolyploidy, and allopolyploidy are observed in the genus. These can lead to misidentifications and taxonomic problems. DNA barcoding studies are important for species identification and solving taxonomic problems. However, preferred DNA sequences may have different effects among plant groups. Therefore, which DNA regions should be preferred is important to obtain more comprehensive results. In this research, psbA-trnH IGS sequences belonging to the Potentilla taxa were examined based on their compatibility and analyzed variable sites, passim-info sites, and nucleotide frequencies (%) for the region relevant by using Molecular Evolutionary Genetics Analysis (MEGA 11). Finally, a Maximum Parsimony (MP) dendrogram was performed to evaluate the Potentilla taxa taxonomically and phylogenetically. As a result of this study, it can be stated that the taxa belonging to the genus Potentilla were well grouped in the dendrogram and the use of psbA-trnH IGS sequences is strongly recommended for further studies.

Keywords

Barkodlama Bölgesi Olarak Potentilla L. Cinsinde psbA-trnH IGS Sekanslarının Değerlendirmesi

Öz

Potentilla cinsi 400’den fazla türle, morfolojik değişimlere neden olan geniş bir yayılışa sahiptir. Geniş yayılışın yanı sıra, cinsteki hibridizasyon, introgression, otopoliploidi ve allopoliploidi gibi birçok durum gözlemlenir. Bunlar, yanlış tanımlamalara ve taksonomik problemlere yol açabilir. DNA barkodlama çalışmaları, tür tanımlama ve taksonomik problemlerin çözümünde önemlidir. Ancak, tercih edilen DNA sekansları, bitki grupları arasında farklı etkilere sahip olabilir. Bu nedenle, hangi DNA bölgelerinin tercih edilmesi gerektiği daha kapsamlı sonuçlar elde etmek için önemlidir. Bu çalışmada, Potentilla taksonlarına ait psbA-trnH IGS sekansları uyumlulukları açısından incelendi ve variable bölgeler, parsim-info bölgeler ve ilgili bölge için nükleotit frekansları (%) Molecular Evolutionary Genetics Analysis (MEGA 11) kullanılarak analiz edildi. Son olarak, Maximum Parsimony (MP) dendrogram taksonomik ve filogenetik olarak Potentilla taksonlarını değerlendirmek için oluşturuldu. Çalışmanın sonucu olarak Potentilla cinsine ait taksonların dendrogramda iyi gruplandığı ve psbA-trnH IGS sekanslarının daha sonraki çalışmalarda kullanımının fayda sağlayacağı söylenebilir.

Anahtar Kelimeler

Teşekkür

The author would like to thank to NCBI for sequence information used in this study and the authors sharing the sequence informations in NCBI.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Biyoteknolojisi , Bitki Hücresi ve Moleküler Biyoloji , Bitki Bilimi (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Aykut Yılmaz ^*
0000-0002-0327-8388
Türkiye

Erken Görünüm Tarihi

23 Nisan 2024

Yayımlanma Tarihi

15 Ağustos 2024

Gönderilme Tarihi

18 Temmuz 2023

Kabul Tarihi

3 Aralık 2023

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1329172

IZ

https://izlik.org/JA38MM46BK

Kaynak Göster

RIS / Bibtex

APA

Yılmaz, A. (2024). The Evaluation of psbA-trnH IGS Sequences in The Genus Potentilla L. as Barcoding Region. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(4), 773-781. https://doi.org/10.18016/ksutarimdoga.vi.1329172

Cited By

Comparative phylogenetic analysis of key chloroplast and nuclear DNA regions of some Brassica species

Genetic Resources and Crop Evolution

https://doi.org/10.1007/s10722-025-02458-0

The Evaluation of psbA-trnH IGS Sequences in The Genus Potentilla L. as Barcoding Region

Abstract

Keywords

Barkodlama Bölgesi Olarak Potentilla L. Cinsinde psbA-trnH IGS Sekanslarının Değerlendirmesi

Öz

Anahtar Kelimeler

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Comparative phylogenetic analysis of key chloroplast and nuclear DNA regions of some Brassica species

e-ISSN: 2619-9149