β-Galactoside α 2-6 Sialyltransferase Gene Expression in Bombyx mori Tissues

Ceren Ersoy; Savaş İzzetoğlu; Gamze Turgay İzzetoğlu

doi:10.18016/ksutarimdoga.vi.1430234

EN TR

β-Galactoside α 2-6 Sialyltransferase Gene Expression in Bombyx mori Tissues

Abstract

Bombyx mori is an important insect due to its genome, homology to humans, and ease of growth. Sialylation has been reported in some insects, but sialic acid biosynthesis cannot be observed in these insects. Sialic acids are negatively charged nine-carbon sugars located terminally on glycoconjugates. Sialylation, which occurs after translation and is regulated by enzymes, has been studied in prokaryotes, deuterostomes, and protostomes. One of the enzymes that is important for sialylation is sialyltransferases. This enzyme plays a role in linking sialic acid to glycoconjugates. In this study, we investigated sialyltransferase (β-Galactoside α-2,6-Sialyltransferase I) gene expression in tissues of B. mori. B. mori larvae, which were fed with fresh mulberry leaves since hatching, were divided into two groups; the control group, which continued to be fed with fresh mulberry leaves in the 5th instar (last instar) and the treatment group, which was fed with mulberry leaves treated with a sialic acid solution. Midgut, fat body, hemocyte, ovary, and testicular tissues were dissected, and gene expressions were examined with Real-Time PCR. The expression level is observed in every tissue, but an increase is seen in only the fat body. The fat body is a vital tissue for insects and plays a fundamental role in immunity, endocrine, and detoxification processes. The reason for the highest gene expression in the fat body can be attributed to the similarities in the functions of the fat body and sialic acid and their roles in insects.

Keywords

Bombyx mori Dokularında β-Galaktozit α 2-6 Siyaliltransferaz Geninin İfadesi

Öz

Bombyx mori genomunun bilinmesi, insanlarla homoloji göstermesi ve kolay yetiştirilmesi açısından en önemli böcek türlerinden biridir. Bazı böceklerde sialilasyona ilişkin raporlar mevcuttur, hatta sialile edilmiş moleküllerin çeşitli yapıları bildirilmiştir ancak böceklerde sialik asit biyosentezi gözlenmemektedir. Sialik asitler, terminal olarak glikoproteinler ve glikolipitler üzerinde bulunan negatif yüklü dokuz karbonlu şekerlerdir. Sialilasyon, enzimler tarafından düzenlenen bir translasyon sonrası modifikasyondur. Prokaryotlar, dueterostomlar ve protostomlarda çalışılmıştır. Sialilasyon için önemli olan enzimlerden biri de sialiltransferazlardır. Bu enzim, sialik asidin glikokonjugatlara bağlanmasında rol oynar. Bu çalışmada ipek böceği Bombyx mori dokularında sialiltransferaz (β-Galaktozit α 2-6 Sialiltransferaz) geninin ifade düzeylerinin belirlenmesidir. Yumurtadan çıkışından itibaren taze dut yaprakları ile beslenen B. mori larvaları, 5. instar (son instar)’da taze dut yaprakları ile beslemeye devam edilen (kontrol grubu) ve sentetik sialik asit solüsyonu uygulanmış dut yaprakları ile beslenilen uygulama grubu olarak ayrıldı. 5. instarın orta bağırsak, yağ doku, hemosit, ovayum ve testis dokuları alınarak Real-Time PZR ile gen ifade düzeyleri incelendi. Ekspresyon düzeyi her doku için ayrı ayrı belirlendi, ancak artış sadece yağ dokuda tespit edildi. Yağ doku böcekler için oldukça önemlidir, bağışıklık, endokrin ve detoksifikasyon süreçlerinde temel rol oynar. Yağ dokuda sialiltransferaz gen ekspresyonunun en fazla olmasının sebebinin yağ doku ve sialik asidin fonksiyonlarındaki benzerliklere ve böceklerdeki rollerine bağlanabilir.

Anahtar Kelimeler

Supporting Institution

Ege University

Project Number

FYL-2021-23087

Thanks

This work was supported by the Ege University Scientific Research Fund (FYL-2021-23087).

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Details

Primary Language

English

Subjects

Entomology , Animal Physiology - Cell , Animal Cell and Molecular Biology

Journal Section

Research Article

Authors

Ceren Ersoy
0000-0002-6408-2987
Türkiye

Savaş İzzetoğlu
0000-0002-1546-1083
Türkiye

Gamze Turgay İzzetoğlu ^*
0000-0001-9828-2402
Türkiye

Early Pub Date

July 2, 2024

Publication Date

September 17, 2024

Submission Date

February 1, 2024

Acceptance Date

March 29, 2024

Published in Issue

Year 2024 Volume: 27 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1430234

IZ

https://izlik.org/JA46TF45FJ

Cite

RIS / Bibtex

APA

Ersoy, C., İzzetoğlu, S., & Turgay İzzetoğlu, G. (2024). β-Galactoside α 2-6 Sialyltransferase Gene Expression in Bombyx mori Tissues. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(5), 1175-1182. https://doi.org/10.18016/ksutarimdoga.vi.1430234