β-Galactoside α 2-6 Sialyltransferase Gene Expression in Bombyx mori Tissues

Ceren Ersoy; Savaş İzzetoğlu; Gamze Turgay İzzetoğlu

doi:10.18016/ksutarimdoga.vi.1430234

EN TR

β-Galactoside α 2-6 Sialyltransferase Gene Expression in Bombyx mori Tissues

Abstract

Bombyx mori is an important insect due to its genome, homology to humans, and ease of growth. Sialylation has been reported in some insects, but sialic acid biosynthesis cannot be observed in these insects. Sialic acids are negatively charged nine-carbon sugars located terminally on glycoconjugates. Sialylation, which occurs after translation and is regulated by enzymes, has been studied in prokaryotes, deuterostomes, and protostomes. One of the enzymes that is important for sialylation is sialyltransferases. This enzyme plays a role in linking sialic acid to glycoconjugates. In this study, we investigated sialyltransferase (β-Galactoside α-2,6-Sialyltransferase I) gene expression in tissues of B. mori. B. mori larvae, which were fed with fresh mulberry leaves since hatching, were divided into two groups; the control group, which continued to be fed with fresh mulberry leaves in the 5th instar (last instar) and the treatment group, which was fed with mulberry leaves treated with a sialic acid solution. Midgut, fat body, hemocyte, ovary, and testicular tissues were dissected, and gene expressions were examined with Real-Time PCR. The expression level is observed in every tissue, but an increase is seen in only the fat body. The fat body is a vital tissue for insects and plays a fundamental role in immunity, endocrine, and detoxification processes. The reason for the highest gene expression in the fat body can be attributed to the similarities in the functions of the fat body and sialic acid and their roles in insects.

Keywords

Bombyx mori Dokularında β-Galaktozit α 2-6 Siyaliltransferaz Geninin İfadesi

Öz

Bombyx mori genomunun bilinmesi, insanlarla homoloji göstermesi ve kolay yetiştirilmesi açısından en önemli böcek türlerinden biridir. Bazı böceklerde sialilasyona ilişkin raporlar mevcuttur, hatta sialile edilmiş moleküllerin çeşitli yapıları bildirilmiştir ancak böceklerde sialik asit biyosentezi gözlenmemektedir. Sialik asitler, terminal olarak glikoproteinler ve glikolipitler üzerinde bulunan negatif yüklü dokuz karbonlu şekerlerdir. Sialilasyon, enzimler tarafından düzenlenen bir translasyon sonrası modifikasyondur. Prokaryotlar, dueterostomlar ve protostomlarda çalışılmıştır. Sialilasyon için önemli olan enzimlerden biri de sialiltransferazlardır. Bu enzim, sialik asidin glikokonjugatlara bağlanmasında rol oynar. Bu çalışmada ipek böceği Bombyx mori dokularında sialiltransferaz (β-Galaktozit α 2-6 Sialiltransferaz) geninin ifade düzeylerinin belirlenmesidir. Yumurtadan çıkışından itibaren taze dut yaprakları ile beslenen B. mori larvaları, 5. instar (son instar)’da taze dut yaprakları ile beslemeye devam edilen (kontrol grubu) ve sentetik sialik asit solüsyonu uygulanmış dut yaprakları ile beslenilen uygulama grubu olarak ayrıldı. 5. instarın orta bağırsak, yağ doku, hemosit, ovayum ve testis dokuları alınarak Real-Time PZR ile gen ifade düzeyleri incelendi. Ekspresyon düzeyi her doku için ayrı ayrı belirlendi, ancak artış sadece yağ dokuda tespit edildi. Yağ doku böcekler için oldukça önemlidir, bağışıklık, endokrin ve detoksifikasyon süreçlerinde temel rol oynar. Yağ dokuda sialiltransferaz gen ekspresyonunun en fazla olmasının sebebinin yağ doku ve sialik asidin fonksiyonlarındaki benzerliklere ve böceklerdeki rollerine bağlanabilir.

Anahtar Kelimeler

Destekleyen Kurum

Ege University

Proje Numarası

FYL-2021-23087

Teşekkür

This work was supported by the Ege University Scientific Research Fund (FYL-2021-23087).

Kaynakça

Altmann, F., Staudacher, E., Wilson, I.B., & Marz, L. (1999). Insect cells as hosts for the expression of recombinant glycoproteins. Glycoconjugate Journal, 16, 109–123.
Alves, S.N., Serrão, J.E., & Melo, A.L. (2010). Alterations in the fat body and midgut of Culex quinquefasciatus larvae following exposure to different insecticides. Micron, 41, 592–597.
Arrese, E.L., & Soulages, J.L. (2010). Insect fat body: Energy, metabolism, and regulation. Annual Review of Entomology, 55, 207-225.
Assis, de W.A., Malta, J., Pimenta, P.F.P., Ramalho-Ortigão, J.M., & Martins, G.F. (2014). The characterization of the fat bodies and oenocytes in the adult females of the sand fly vectors Lutzomyia longipalpis and Phlebotomus papatasi. Arthropod Structure and Development, 43, 501–509.
Buschiazzo, A., & Alzari, P.M. (2008). Sialic acid metabolism structural insights into sialic acid enzymology. Current Opinion in Chemical Biology, 12, 565–572.
Chapman, R.F. (2013). Chapter 6 – Fat body (Part I. The head, ingestion, utilization and distribution of food). The Insects Structure and Function, fifth ed. Cambridge University Press, New York, 132–144 pp.
Cime-Castillo, J., Delannoy, P., Mendoza-Hernández, G., Monroy-Martínez, V., Harduin-Lepers, A., Lanz- Mendoza, H., Hernández-Hernández, Fde L., Zenteno, Cabello-Gutiérrez, C., & Ruiz-Ordaz, B.H. (2014). Sialic acid expression in the mosquito Aedes aegypti and its possible role in dengue virus-vector interactions. Hindawi Publishing Corporation BioMed Research International, 16.
Feitosa, F.M., Calvo, E., Merino, E.F., Durham, A.M., James, A.A., Bianchi, A.G., Marinotti, O., & Capurro, M.L. (2006). A transcriptome analysis of the Aedes aegypti vitellogenic fat body. Journal of Insect Science, 6, 1–26.

Ghosh, S. (2018). Sialylation and sialyltransferase in insects, Glycoconjugate Journal, 35, 433-441. (Mini-Review)
Hsu, T.A., Takahashi, N., Tsukamoto, Y., Kato, K., Shimada, I., Masuda, K., Whiteley, E.M., Fan, J.Q., Lee, Y.C., & Betenbaugh, M.J. (1997). Differential n-glycan patterns of secreted and intracellular igg produced in trichoplusia in cells. Journal of Biological Chemistry, 272, 9062–9070.
Kajiura, H., Hamaguchi, Y., Mizushima, H., Misaki, R., & Fujiyama, K. (2015). Sialylation potentials of the silkworm, Bombyx mori, B. mori possesses an active α2, 6-sialyltransferase. Glycobiology, 25(12), 1441-1453.
Koles, K., Irvine, K.D., & Panin, V.M. (2004). Functional characterization of Drosophila sialyltransferase, Journal of Biological Chemistry, 6, 4346-4357.
Koles, K., Lim, J.M., Aoki, K., Porterfield, M., Tiemeyer, M., Wells, L., & Panin, V. (2007). Identification of n-glycosylated proteins from the central nervous system of Drosophila melanogaster. Glycobiology, 17(12), 1388-1403.
Law, J.H., & Wells, M.A. (1989). Insects as biochemical models. Journal of Biological Chemistry, 264, 16335–16338.
Lawrence, S.M., Huddleston, K.A., Tomiya, N., Nguyen, N., Lee, Y.C., Vann, W.F., Coleman, T.A., & Betenbaugh, M.J. (2001). Cloning and expression of human sialic acid pathway genes to generate CMP-sialic acids in insect cells. Glycoconjugate Journal, 18, 205-213.
Li, Y. & Chen, X. (2012). Sialic acid metabolism and sialyltransferases: Natural functions and applications. Applied Microbiology & Biotechnology, 94(4), 887-905.
Malykh, Y.N., Krisch, B., Gerardy-Schahn, R., Lapina, E.B., Shaw, L., & Schauer, R. (1999). The presence of n-acetylneuraminic acid in malpighian tubules of larvae of the Cicada Philaenus spumarius, Glycoconjugate Journal, 16(11), 731-739.
Marchal, I., Jarvis, D.L., Cacan, R. & Verbert, A. (2001). Glycoproteins from insect cells: sialylated or not? Biological Chemistry, 382, 151-159.
Martins, G.F., Ramalho-Ortigao, J.M. (2012). Oenocytes in Insects. Invertebrate Survival Journal, 9, 139–152.
Meng, X., Zhu, F., & Chen, K. (2017). Silkworm: a promising model organism in life science. Journal of Insect Science, 17(5), 97.
Miyazaki, T., Miyashita, R., Nakamura, S., Ikegaya, M., Kato, T. & Park, E.Y. (2019). Biochemical characterization and mutational analysis of silkworm Bombyx mori Β-1, 4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of β-1, 4-galactosyltransferase family enzymes. Insect Biochemistry and Molecular Biology, 115, 103254.
Nation, J.L. (2016). Insect Physiology and Biochemistry, 3rd ed. CRC Press, USA.
Park, M.S., Park, P., & Takeda, M. (2013). Roles of fat body trophocytes, mycetocytes and oocytes in the American cockroach, Periplaneta americana under starvation conditions: an ultrastructural study. The journal Arthropod Structure & Development, 42, 287–295.
Petit, D., Teppa, E., Mir, A.M., Vicogne, D., Thisse, C., Thisse, B., Filloux, C., & Harduin-Lepers, A. (2015). An integrative view of α2, 3-sialyltransferases (ST3Gal) molecular and functional evolution in deuterostomes: significance of lineage-specific losses. Molecular Biology & Evolution, 32(4), 906-927.
Repnikova, E., Koles, K., Nakamura, M., Pitts, J., Li, H., Ambavane, A., Zoran, M.J., & Panin, V.M. (2010). Sialyltransferase regulates nervous system function in Drosophila. Journal of Neuroscience, 30, 6466–6476.
Resh, V.H., & Cardé, R.T. (Eds.). (2009). Encyclopedia of insects. Academic press. 103-408.
Roma, G.C., Bueno, O.C., Camargo-Mathias, M.I. (2010). Morpho-physiological analysis of the insect fat body: A review. Micron, 41(5), 395-401.
Schauer, R. (2001). The occurrence and significance of sialic acids in insects. Trends in Glycoscience & Glycotechnology, 13, 507-17.
Schauer, R. (2009). Sialic acids as regulators of molecular and cellular interactions. Current Opinion in Structural Biology, 19, 507–514.
Shimomura, M., Minami, H., Suetsugu, Y., Ohyanagi, H., Satoh, C., Antonio, B., Nagamura, Y., Kadono-Okuda, K., Kajiwara, H., Sezutsu, H., Nagaraju, J., Goldsmith, M.R., Xia, Q., Yamamoto, K., & Mita, K. (2009). KAIKObase: an integrated silkworm genome database and data mining tool. BMC Genomics, 10(1), 1-8.
Teppa, R.E., Petit, D., Plechakova, O., Cogez, V. & Harduin-Lepers, A. (2016). Phylogenetic-derived insights into the evolution of sialylation in eukaryotes: comprehensive analysis of vertebrate β-galactoside α2, 3/6-sialyltransferases (ST3Gal and ST6Gal). International Journal of Molecular Sciences, 17(8), 1286.
Turgay-İzzetoğlu, G. & Gülmez, M. (2019). Characterization of fat body cells at different developmental stages of Culex pipiens. Acta Histochemica, 121, 460–471.
Tutar, E., Köksalan, E. & Akyol, İ. (2015). Gıdalarda Bulunan Mikrobiyal Patojenlerin Karakterizasyonunda Real Time PCR Teknolojisi. KSÜ Tarım ve Doğa Dergisi. 18(4), 26-39.
Varki, A. (2017). Biological roles of glycans. Glycobiology, 27(1), 3-49.
Weinstein, J., Lee, E.U., McEntee, K., Lai, P.H. & Paulson, J.C. (1987). Primary structure of beta-galactoside alpha 2, 6-sialyltransferase. Conversion of membrane-bound enzyme to soluble forms by cleavage of the NH2-terminal signal anchor. Journal of Biological Chemistry, 262(36), 17735-17743.
Yıldırım, F., Özdemir, S. & Yıldız, A. (2018). Koçaş Tarım İşletmesinde Yetiştirilen Siyah Alaca (Holştayn) Sığırlarda Bazı Süt Verimi Özellikleri ve İlişkili Genlerin Ekspresyonu. KSÜ Tarım ve Doğa Dergisi, 21(3), 353-362.
Zhang, Y., Xi, Y. (2014). Fat body development and its function in energy storage and nutrient sensing in Drosophila melanogaster. Journal of Tissue Science & Engineering, 6(1), 1–8.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Entomoloji , Hayvan Fizyolojisi - Hücre , Hayvan Hücresi ve Moleküler Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Ceren Ersoy
0000-0002-6408-2987
Türkiye

Savaş İzzetoğlu
0000-0002-1546-1083
Türkiye

Gamze Turgay İzzetoğlu ^*
0000-0001-9828-2402
Türkiye

Erken Görünüm Tarihi

2 Temmuz 2024

Yayımlanma Tarihi

17 Eylül 2024

Gönderilme Tarihi

1 Şubat 2024

Kabul Tarihi

29 Mart 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1430234

IZ

https://izlik.org/JA46TF45FJ

Kaynak Göster

RIS / Bibtex

APA

Ersoy, C., İzzetoğlu, S., & Turgay İzzetoğlu, G. (2024). β-Galactoside α 2-6 Sialyltransferase Gene Expression in Bombyx mori Tissues. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(5), 1175-1182. https://doi.org/10.18016/ksutarimdoga.vi.1430234

β-Galactoside α 2-6 Sialyltransferase Gene Expression in Bombyx mori Tissues

Abstract

Keywords

Bombyx mori Dokularında β-Galaktozit α 2-6 Siyaliltransferaz Geninin İfadesi

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

e-ISSN: 2619-9149