Tokat İli Patates Üretim Alanlarında Patates Yumrularında Enfeksiyon Oluşturan Virüslerin Moleküler Olarak Tespiti

Afide Merve Engür; Şerife Topkaya

doi:10.18016/ksutarimdoga.vi.1435134

TR EN

Tokat İli Patates Üretim Alanlarında Patates Yumrularında Enfeksiyon Oluşturan Virüslerin Moleküler Olarak Tespiti

Öz

Tokat ilinde patates yetiştiriciliği yapılan Niksar, Erbaa ve Artova ilçelerindeki patates üretim alanlarından virüs belirtisi gösteren patates bitkilerinden 91 adet yumru örneği toplanmıştır. Yumru örnekleri, potato virus Y (PVY), potato virus S (PVS), potato virus X (PVX) ve potato leafroll virus (PLRV) etmenlerinin varlığını tespit etmek amacıyla RT-PCR testine tabi tutulmuştur. Test edilen yumru örneklerinin 68 tanesinde (%74.72) tekli veya karışık virüs enfeksiyonun varlığı tespit edilmiştir. Çimlendirilen 91 yumrunun 67’si PVY (%73.62), 6’sı PVS (%6.59) ve 1 tanesi de PLRV (%1.09) ile enfekteli bulunmuştur. Ayrıca PVS ile enfekteli bulunan yumrulardan 5 tanesinde PVY+PVS karışık enfeksiyonu belirlenirken, PLRV ile enfekteli yumruda da PVY enfeksiyonu tespit edilmiştir. Tokat ilinde farklı ilçelerden toplanan yumrularda en yaygın PVY görülürken bunu PVS’ etmeninin izlediği belirlenmiştir. Tohumluk olarak kullanılan enfekteli yumrular yıldan yıla verimde ciddi kayıplara neden olmaktadır. Virüsten ari ve sertifikalı tohumlukların kullanılması viral kaynaklı kaybın önüne geçilmesinde en önemli faktördür.

Anahtar Kelimeler

Molecular Detection of Infectious Viruses in Potato Tubers in Potato Production Areas in Tokat Province

Abstract

Ninety-one potato tuber samples showing virus symptoms were collected from potato production areas in Niksar, Erbaa, and Artova districts, where potato cultivation is done in Tokat province. Tuber samples were subjected to RT-PCR tests to determine the presence of potato virus Y (PVY), potato virus S (PVS), potato virus X (PVX), and potato leafroll virus (PLRV) viruses. Single or mixed virus infections were detected in 68 (74.72%) of the tuber samples tested. Of the 91 germinated tubers, 67 were infected with PVY (73.62%), 6 were infected with PVS (6.59%), and one was infected with PLRV (1.09%). In addition, PVY+PVS mixed infection was detected in 5 of the tubers infected with PVS, while PVY infection was detected in the tubers infected with PLRV. It was determined that the most common virus in tuber samples collected from different districts of Tokat province was PVY, followed by PVS. Infected tubers used as seeds cause serious losses in yield from year to year. The use of virus-free and certified seeds is the most important factor in preventing viral-related losses. otato leafroll virus (PLRV) viruses. Single or mixed virus infections were detected in 68 (74.72%) of the tuber samples tested.

Keywords

Supporting Institution

Tokat Gaziosmanpaşa Üniversitesi Bilimsel Araştırma Projeler Koordinatörlüğü

Project Number

2018/50

References

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Details

Primary Language

Turkish

Subjects

Phytopathology

Journal Section

Research Article

Authors

Afide Merve Engür
0000-0002-5874-4016
Türkiye

Şerife Topkaya ^*
0000-0002-0095-474X
Türkiye

Early Pub Date

July 2, 2024

Publication Date

September 17, 2024

Submission Date

February 11, 2024

Acceptance Date

April 30, 2024

Published in Issue

Year 2024 Volume: 27 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1435134

IZ

https://izlik.org/JA26JB92AU

Cite

RIS / Bibtex

APA

Engür, A. M., & Topkaya, Ş. (2024). Tokat İli Patates Üretim Alanlarında Patates Yumrularında Enfeksiyon Oluşturan Virüslerin Moleküler Olarak Tespiti. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(5), 1061-1068. https://doi.org/10.18016/ksutarimdoga.vi.1435134

Cited By

Phylogenetic and Population Analyses on P1, HC-Pro, and P3 of Potato Virus Y Isolates Infecting Potato in the Central Region of Türkiye

Potato Research

https://doi.org/10.1007/s11540-025-09938-x