Detection of the Presence of Powdery Mildew Resistance -Associated Genes (Ren1, Ren3, and Ren9) in Vitis labrusca L. Genotypes

Adem Yağcı; Selda Daler; Abdurrahim Bozkurt; Davut Soner Akgül

doi:10.18016/ksutarimdoga.vi.1454506

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Vitis labrusca L. Genotiplerinde Küllemeye Dirençle İlişkili Genlerin (Ren1, Ren3 ve Ren9) Varlığının Tespiti

Öz

Bağlarda görülen külleme hastalığı (Erysiphe necator Schwein) üzüm yetiştiriciliği için büyük bir tehdittir. Bu patojene karşı mücadele edilmediğinde, üzüm verim ve kalitesinde önemli düşüşler meydana gelmektedir. Çoğu ticari üzüm çeşidi, küllemeye karşı oldukça hassastır. Bu nedenle yetiştirme dönemi boyunca fazla miktarlarda fungisit uygulanmaktadır. Sürdürülebilir bir bağcılık için verim ve kalitenin yanında, külleme hastalığına karşı genetik olarak dirençli yeni asma çeşitlerine ihtiyaç duyulmaktadır. Bu çalışmada, Türkiye’nin Karadeniz Bölgesi’nde yetiştiriciliği yapılan dokuz farklı Vitis labrusca L. genotipinde (TEG-Vl-1, TEG-Vl-2, TEG-Vl-3, TEG-Vl-4, TEG-Vl-5, TEG-Vl-6, TEG-Vl-7, TEG-Vl-8 ve TEG-Vl-9) külleme hastalığına dirençli genlerin moleküler taraması yapılmıştır. Ren1, Ren3 ve Ren9 lokuslarına özgü primerler kullanılarak gerçekleştirilen PCR amplifikasyonu sonrasında üç genotipte (TEG-Vl-1, TEG-Vl-3 ve TEG-Vl-4) Ren1 ve Ren9 genlerinin bulunduğu tespit edilmiştir. Ancak Ren3 geni hiçbir örnekte saptanamamıştır. Araştırma sonuçlarında direnç genlerine sahip oldukları belirlenen Vitis labrusca L. genotiplerinin, gelecekte dirençli asma çeşitlerinin ıslahında genetik kaynaklar olarak kullanılabilecekleri ve bu sayede önemli ekonomik faydalar sağlanabileceği düşünülmektedir.

Anahtar Kelimeler

Detection of the Presence of Powdery Mildew Resistance -Associated Genes (Ren1, Ren3, and Ren9) in Vitis labrusca L. Genotypes

Abstract

Powdery mildew disease (Erysiphe necator Schwein) is a significant threat to grape cultivation in vineyards. Severe yield and quality losses could occur in vineyards when this pathogen is not managed correctly. Several commercial grape varieties are highly susceptible to powdery mildew. Therefore, large quantities of fungicides are applied throughout the growing season. In addition to yields and quality, new grapevine varieties that are genetically resistant to powdery mildew are required for sustainable viticulture. This study was conducted through molecular screening of powdery mildew resistance genes in nine different Vitis labrusca L. genotypes (TEG-Vl-1, TEG-Vl-2, TEG-Vl-3, TEG-Vl-4, TEG-Vl-5, TEG-Vl-6, TEG-Vl-7, TEG-Vl-8, and TEG-Vl-9) grown in the Black Sea Region of Türkiye. After PCR amplifications using Ren1, Ren3 and Ren9 locus-specific primers, Ren1, and Ren 9 genes were detected in three genotypes (TEG-Vl-1, TEG-Vl-3, and TEG-Vl-4). However, the Ren3 gene was not detected in any genotypes. It was concluded based on present findings that Vitis labrusca L. genotypes with resistance genes could be used as genetic resources in grapevine breeding programs and significant economic benefits can be provided accordingly.

Keywords

Supporting Institution

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Thanks

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Details

Primary Language

English

Subjects

Oenology and Viticulture

Journal Section

Research Article

Authors

Adem Yağcı ^*
0000-0002-3650-4679
Türkiye

Selda Daler
0000-0003-0422-1444
Türkiye

Abdurrahim Bozkurt
0000-0001-7315-202X
Türkiye

Davut Soner Akgül
0000-0002-9990-4194
Türkiye

Early Pub Date

September 15, 2024

Publication Date

December 25, 2024

Submission Date

March 17, 2024

Acceptance Date

May 14, 2024

Published in Issue

Year 2024 Volume: 27 Number: Ek Sayı 1 (Suppl 1)

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1454506

IZ

https://izlik.org/JA83ZU62KL

Cite

RIS / Bibtex

APA

Yağcı, A., Daler, S., Bozkurt, A., & Akgül, D. S. (2024). Detection of the Presence of Powdery Mildew Resistance -Associated Genes (Ren1, Ren3, and Ren9) in Vitis labrusca L. Genotypes. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1), 163-173. https://doi.org/10.18016/ksutarimdoga.vi.1454506