Detection of the Presence of Powdery Mildew Resistance -Associated Genes (Ren1, Ren3, and Ren9) in Vitis labrusca L. Genotypes

Adem Yağcı; Selda Daler; Abdurrahim Bozkurt; Davut Soner Akgül

doi:10.18016/ksutarimdoga.vi.1454506

TR EN

Vitis labrusca L. Genotiplerinde Küllemeye Dirençle İlişkili Genlerin (Ren1, Ren3 ve Ren9) Varlığının Tespiti

Öz

Bağlarda görülen külleme hastalığı (Erysiphe necator Schwein) üzüm yetiştiriciliği için büyük bir tehdittir. Bu patojene karşı mücadele edilmediğinde, üzüm verim ve kalitesinde önemli düşüşler meydana gelmektedir. Çoğu ticari üzüm çeşidi, küllemeye karşı oldukça hassastır. Bu nedenle yetiştirme dönemi boyunca fazla miktarlarda fungisit uygulanmaktadır. Sürdürülebilir bir bağcılık için verim ve kalitenin yanında, külleme hastalığına karşı genetik olarak dirençli yeni asma çeşitlerine ihtiyaç duyulmaktadır. Bu çalışmada, Türkiye’nin Karadeniz Bölgesi’nde yetiştiriciliği yapılan dokuz farklı Vitis labrusca L. genotipinde (TEG-Vl-1, TEG-Vl-2, TEG-Vl-3, TEG-Vl-4, TEG-Vl-5, TEG-Vl-6, TEG-Vl-7, TEG-Vl-8 ve TEG-Vl-9) külleme hastalığına dirençli genlerin moleküler taraması yapılmıştır. Ren1, Ren3 ve Ren9 lokuslarına özgü primerler kullanılarak gerçekleştirilen PCR amplifikasyonu sonrasında üç genotipte (TEG-Vl-1, TEG-Vl-3 ve TEG-Vl-4) Ren1 ve Ren9 genlerinin bulunduğu tespit edilmiştir. Ancak Ren3 geni hiçbir örnekte saptanamamıştır. Araştırma sonuçlarında direnç genlerine sahip oldukları belirlenen Vitis labrusca L. genotiplerinin, gelecekte dirençli asma çeşitlerinin ıslahında genetik kaynaklar olarak kullanılabilecekleri ve bu sayede önemli ekonomik faydalar sağlanabileceği düşünülmektedir.

Anahtar Kelimeler

Detection of the Presence of Powdery Mildew Resistance -Associated Genes (Ren1, Ren3, and Ren9) in Vitis labrusca L. Genotypes

Abstract

Powdery mildew disease (Erysiphe necator Schwein) is a significant threat to grape cultivation in vineyards. Severe yield and quality losses could occur in vineyards when this pathogen is not managed correctly. Several commercial grape varieties are highly susceptible to powdery mildew. Therefore, large quantities of fungicides are applied throughout the growing season. In addition to yields and quality, new grapevine varieties that are genetically resistant to powdery mildew are required for sustainable viticulture. This study was conducted through molecular screening of powdery mildew resistance genes in nine different Vitis labrusca L. genotypes (TEG-Vl-1, TEG-Vl-2, TEG-Vl-3, TEG-Vl-4, TEG-Vl-5, TEG-Vl-6, TEG-Vl-7, TEG-Vl-8, and TEG-Vl-9) grown in the Black Sea Region of Türkiye. After PCR amplifications using Ren1, Ren3 and Ren9 locus-specific primers, Ren1, and Ren 9 genes were detected in three genotypes (TEG-Vl-1, TEG-Vl-3, and TEG-Vl-4). However, the Ren3 gene was not detected in any genotypes. It was concluded based on present findings that Vitis labrusca L. genotypes with resistance genes could be used as genetic resources in grapevine breeding programs and significant economic benefits can be provided accordingly.

Keywords

Destekleyen Kurum

Yok

Etik Beyan

Yok

Teşekkür

Tokat ili Erbaa ilçesi bağcılarına teşekkür ederiz.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Şarapçılık ve Bağcılık

Bölüm

Araştırma Makalesi

Yazarlar

Adem Yağcı ^*
0000-0002-3650-4679
Türkiye

Selda Daler
0000-0003-0422-1444
Türkiye

Abdurrahim Bozkurt
0000-0001-7315-202X
Türkiye

Davut Soner Akgül
0000-0002-9990-4194
Türkiye

Erken Görünüm Tarihi

15 Eylül 2024

Yayımlanma Tarihi

25 Aralık 2024

Gönderilme Tarihi

17 Mart 2024

Kabul Tarihi

14 Mayıs 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: Ek Sayı 1 (Suppl 1)

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1454506

IZ

https://izlik.org/JA83ZU62KL

Kaynak Göster

RIS / Bibtex

APA

Yağcı, A., Daler, S., Bozkurt, A., & Akgül, D. S. (2024). Detection of the Presence of Powdery Mildew Resistance -Associated Genes (Ren1, Ren3, and Ren9) in Vitis labrusca L. Genotypes. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1), 163-173. https://doi.org/10.18016/ksutarimdoga.vi.1454506

Vitis labrusca L. Genotiplerinde Küllemeye Dirençle İlişkili Genlerin (Ren1, Ren3 ve Ren9) Varlığının Tespiti

Öz

Anahtar Kelimeler

Detection of the Presence of Powdery Mildew Resistance -Associated Genes (Ren1, Ren3, and Ren9) in Vitis labrusca L. Genotypes

Abstract

Keywords

Destekleyen Kurum

Etik Beyan

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

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