Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts

Bayram Yurt; Rüya Sağlamtaş; Yeliz Demir; Ebubekir İzol; Halit Diril; Cüneyt Çağlayan

doi:10.18016/ksutarimdoga.vi.1472403

TR EN

Bazı Tıbbi ve Aromatik Bitki Ekstraktlarının in vitro Antioksidan, Antikolinerjik ve Antiepileptik Aktivitelerinin Belirlenmesi

Abstract

Crocus cancellatus, Scilla siberica subsp. armena, Juniperus oxycedrus subsp. oxycedrus ve Anthriscus nemorosa gibi tıbbi ve aromatik bitkiler birçok farklı biyolojik aktiviteye sahiptir. Antioksidanlar birçok hastalığın önlenmesinde önemli rol oynarken, metabolik enzimlerin inhibisyonu da birçok hastalığın önlenmesinde etkilidir. Bu çalışmada, dört farklı tıbbi ve aromatik bitki türünün su, etanol ve diklorometan ekstraktlarının antioksidan aktiviteleri 1,1-difenil-2-pikrilhidrazil (DPPH•) ve 2,20-azino-bis-3-etilbenzthiazoline-6-sülfonik asit (ABTS•+) radikal giderme ve Cu2+, Fe3+ ve Fe3+-TPTZ indirgeme deneyleri ile belirlenmiştir. Enzim inhibisyon çalışmaları metabolik enzimler olan asetilkolinesteraz, bütirilkolinesteraz, karbonik anhidraz I ve II izoenzimleri ile gerçekleştirilmiştir. A. nemorosa'nın etanol ekstresi DPPH ve ABTS deneylerinde en yüksek aktiviteyi göstermiştir (IC50: 17.36 µg mL-1, IC50: 7.02 µg mL-1). Fe3+ indirgeme deneyinde, A. nemorosa'nın diklorometan ekstresi en yüksek aktiviteyi göstermiştir (1.96±0.060 µg mL-1). Cu2+ indirgeme deneyinde, J. oxycedrus'un diklorometan ekstresi en yüksek aktiviteyi göstermiştir (1.773±0.066 µg mL-1). Fe3+-TPTZ indirgeme deneyinde, S. siberica'nın etanol ekstraktı en yüksek aktiviteyi göstermiştir (1.256±0.011 µg mL-1). Enzim inhibisyon sonuçlarında, tüm bitkilerin çalışılan enzimleri inhibe ettiği belirlenmiştir. Bu çalışma sonucunda tıbbi ve aromatik bitkilerden olan bu dört bitkinin yüksek biyolojik aktiviteye sahip olduğu belirlenmiştir.

Keywords

Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts

Abstract

Medicinal and aromatic plants such as Crocus cancellatus, Scilla Siberica subsp. armena, Juniperus oxycedrus subsp. oxycedrus and Anthriscus nemorosa have many different biological activities. While antioxidants are significant in preventing many diseases, inhibition of metabolic enzymes is also effective in preventing many diseases. In this study, antioxidant activities of water, ethanol, and dichloromethane extracts of four different medicinal and aromatic plant species were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,20-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS•+) radical scavenging and Cu2+, Fe+3, and Fe3+-2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) reducing assays. Enzyme inhibition studies were performed with metabolic enzymes acetylcholinesterase, butyrylcholinesterase, carbonic anhydrase I and II isoenzymes. The ethanol extract of A. nemorosa showed the highest activity in DPPH and ABTS assays (IC50: 17.36 µg mL-1, IC50: 7.02 µg mL-1). In the Fe3+ reducing assay, the dichloromethane extract of A. nemorosa showed the highest activity (1.96±0.060 µg mL-1). In the Cu2+ reducing assay, the dichloromethane extract of J. oxycedrus showed the highest activity (1.773±0.066 µg mL-1). In the Fe3+-TPTZ reducing assay, the ethanol extract of S. siberica showed the highest activity (1.256±0.011 µg mL-1). In the enzyme inhibition results, it was determined that all plants and all extracts inhibited the enzymes studied. As a result of this study, it was determined that these four medicinal and aromatic plants have high biological activities.

Keywords

Ethical Statement

The study does not require ethics committee declaration.

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Details

Primary Language

English

Subjects

Plant Biochemistry

Journal Section

Research Article

Authors

Bayram Yurt
0000-0001-5447-1586
Türkiye

Rüya Sağlamtaş
0000-0002-4400-2302
Türkiye

Yeliz Demir
0000-0003-3216-1098
Türkiye

Ebubekir İzol ^*
0000-0003-0788-4999
Türkiye

Halit Diril
0000-0003-4409-8268
Türkiye

Cüneyt Çağlayan
0000-0001-5608-554X
Türkiye

Early Pub Date

September 15, 2024

Publication Date

December 25, 2024

Submission Date

April 23, 2024

Acceptance Date

August 9, 2024

Published in Issue

Year 2024 Volume: 27 Number: Ek Sayı 1 (Suppl 1)

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1472403

IZ

https://izlik.org/JA54WW93KZ

Cite

RIS / Bibtex

APA

Yurt, B., Sağlamtaş, R., Demir, Y., İzol, E., Diril, H., & Çağlayan, C. (2024). Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1), 1-15. https://doi.org/10.18016/ksutarimdoga.vi.1472403

AMA

1.Yurt B, Sağlamtaş R, Demir Y, İzol E, Diril H, Çağlayan C. Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts. KSU J. Agric Nat. 2024;27(Ek Sayı 1 (Suppl 1):1-15. doi:10.18016/ksutarimdoga.vi.1472403

Chicago

Yurt, Bayram, Rüya Sağlamtaş, Yeliz Demir, Ebubekir İzol, Halit Diril, and Cüneyt Çağlayan. 2024. “Determination of in Vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of Some Medicinal and Aromatic Plant Extracts”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 27 (Ek Sayı 1 (Suppl 1): 1-15. https://doi.org/10.18016/ksutarimdoga.vi.1472403.

EndNote

Yurt B, Sağlamtaş R, Demir Y, İzol E, Diril H, Çağlayan C (December 1, 2024) Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27 Ek Sayı 1 (Suppl 1) 1–15.

IEEE

[1]B. Yurt, R. Sağlamtaş, Y. Demir, E. İzol, H. Diril, and C. Çağlayan, “Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts”, KSU J. Agric Nat., vol. 27, no. Ek Sayı 1 (Suppl 1), pp. 1–15, Dec. 2024, doi: 10.18016/ksutarimdoga.vi.1472403.

ISNAD

Yurt, Bayram - Sağlamtaş, Rüya - Demir, Yeliz - İzol, Ebubekir - Diril, Halit - Çağlayan, Cüneyt. “Determination of in Vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of Some Medicinal and Aromatic Plant Extracts”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27/Ek Sayı 1 (Suppl 1) (December 1, 2024): 1-15. https://doi.org/10.18016/ksutarimdoga.vi.1472403.

JAMA

1.Yurt B, Sağlamtaş R, Demir Y, İzol E, Diril H, Çağlayan C. Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts. KSU J. Agric Nat. 2024;27:1–15.

MLA

Yurt, Bayram, et al. “Determination of in Vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of Some Medicinal and Aromatic Plant Extracts”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 27, no. Ek Sayı 1 (Suppl 1), Dec. 2024, pp. 1-15, doi:10.18016/ksutarimdoga.vi.1472403.

Vancouver

1.Bayram Yurt, Rüya Sağlamtaş, Yeliz Demir, Ebubekir İzol, Halit Diril, Cüneyt Çağlayan. Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts. KSU J. Agric Nat. 2024 Dec. 1;27(Ek Sayı 1 (Suppl 1):1-15. doi:10.18016/ksutarimdoga.vi.1472403