Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts

Bayram Yurt; Rüya Sağlamtaş; Yeliz Demir; Ebubekir İzol; Halit Diril; Cüneyt Çağlayan

doi:10.18016/ksutarimdoga.vi.1472403

TR EN

Bazı Tıbbi ve Aromatik Bitki Ekstraktlarının in vitro Antioksidan, Antikolinerjik ve Antiepileptik Aktivitelerinin Belirlenmesi

Öz

Crocus cancellatus, Scilla siberica subsp. armena, Juniperus oxycedrus subsp. oxycedrus ve Anthriscus nemorosa gibi tıbbi ve aromatik bitkiler birçok farklı biyolojik aktiviteye sahiptir. Antioksidanlar birçok hastalığın önlenmesinde önemli rol oynarken, metabolik enzimlerin inhibisyonu da birçok hastalığın önlenmesinde etkilidir. Bu çalışmada, dört farklı tıbbi ve aromatik bitki türünün su, etanol ve diklorometan ekstraktlarının antioksidan aktiviteleri 1,1-difenil-2-pikrilhidrazil (DPPH•) ve 2,20-azino-bis-3-etilbenzthiazoline-6-sülfonik asit (ABTS•+) radikal giderme ve Cu2+, Fe3+ ve Fe3+-TPTZ indirgeme deneyleri ile belirlenmiştir. Enzim inhibisyon çalışmaları metabolik enzimler olan asetilkolinesteraz, bütirilkolinesteraz, karbonik anhidraz I ve II izoenzimleri ile gerçekleştirilmiştir. A. nemorosa'nın etanol ekstresi DPPH ve ABTS deneylerinde en yüksek aktiviteyi göstermiştir (IC50: 17.36 µg mL-1, IC50: 7.02 µg mL-1). Fe3+ indirgeme deneyinde, A. nemorosa'nın diklorometan ekstresi en yüksek aktiviteyi göstermiştir (1.96±0.060 µg mL-1). Cu2+ indirgeme deneyinde, J. oxycedrus'un diklorometan ekstresi en yüksek aktiviteyi göstermiştir (1.773±0.066 µg mL-1). Fe3+-TPTZ indirgeme deneyinde, S. siberica'nın etanol ekstraktı en yüksek aktiviteyi göstermiştir (1.256±0.011 µg mL-1). Enzim inhibisyon sonuçlarında, tüm bitkilerin çalışılan enzimleri inhibe ettiği belirlenmiştir. Bu çalışma sonucunda tıbbi ve aromatik bitkilerden olan bu dört bitkinin yüksek biyolojik aktiviteye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts

Abstract

Medicinal and aromatic plants such as Crocus cancellatus, Scilla Siberica subsp. armena, Juniperus oxycedrus subsp. oxycedrus and Anthriscus nemorosa have many different biological activities. While antioxidants are significant in preventing many diseases, inhibition of metabolic enzymes is also effective in preventing many diseases. In this study, antioxidant activities of water, ethanol, and dichloromethane extracts of four different medicinal and aromatic plant species were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,20-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS•+) radical scavenging and Cu2+, Fe+3, and Fe3+-2,4,6-tris(2-pyridyl)-S-triazine (TPTZ) reducing assays. Enzyme inhibition studies were performed with metabolic enzymes acetylcholinesterase, butyrylcholinesterase, carbonic anhydrase I and II isoenzymes. The ethanol extract of A. nemorosa showed the highest activity in DPPH and ABTS assays (IC50: 17.36 µg mL-1, IC50: 7.02 µg mL-1). In the Fe3+ reducing assay, the dichloromethane extract of A. nemorosa showed the highest activity (1.96±0.060 µg mL-1). In the Cu2+ reducing assay, the dichloromethane extract of J. oxycedrus showed the highest activity (1.773±0.066 µg mL-1). In the Fe3+-TPTZ reducing assay, the ethanol extract of S. siberica showed the highest activity (1.256±0.011 µg mL-1). In the enzyme inhibition results, it was determined that all plants and all extracts inhibited the enzymes studied. As a result of this study, it was determined that these four medicinal and aromatic plants have high biological activities.

Keywords

Etik Beyan

The study does not require ethics committee declaration.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Biyokimyası

Bölüm

Araştırma Makalesi

Yazarlar

Bayram Yurt
0000-0001-5447-1586
Türkiye

Rüya Sağlamtaş
0000-0002-4400-2302
Türkiye

Yeliz Demir
0000-0003-3216-1098
Türkiye

Ebubekir İzol ^*
0000-0003-0788-4999
Türkiye

Halit Diril
0000-0003-4409-8268
Türkiye

Cüneyt Çağlayan
0000-0001-5608-554X
Türkiye

Erken Görünüm Tarihi

15 Eylül 2024

Yayımlanma Tarihi

25 Aralık 2024

Gönderilme Tarihi

23 Nisan 2024

Kabul Tarihi

9 Ağustos 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 27 Sayı: Ek Sayı 1 (Suppl 1)

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1472403

IZ

https://izlik.org/JA54WW93KZ

Kaynak Göster

RIS / Bibtex

APA

Yurt, B., Sağlamtaş, R., Demir, Y., İzol, E., Diril, H., & Çağlayan, C. (2024). Determination of in vitro Antioxidant, Anticholinergic, and Antiepileptic Activities of some Medicinal and Aromatic Plant Extracts. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1), 1-15. https://doi.org/10.18016/ksutarimdoga.vi.1472403