Phenolic Profile and Antioxidant Capacity of Helichrysum arenarium Extracts: A Comprehensive LC-MS/MS and Antioxidant Analysis

Yılmaz Uğur; Abdussamat Güzel

doi:10.18016/ksutarimdoga.vi.1545680

TR EN

Helichrysum arenarium Ekstraktlarının Fenolik Profili ve Antioksidan Kapasitesi: Kapsamlı LC-MS/MS ve Antioksidan Analizi

Öz

Helichrysum arenarium, halk arasında ölümsüz veya sonsuz çiçek olarak bilinen, Asteraceae familyasına ait bir bitkidir ve potansiyel tıbbi özellikleriyle tanınmaktadır. Bu çalışmanın amacı, H. arenarium'un fenolik profilini ve antioksidan özelliklerini ileri düzey analitik tekniklerle açıklığa kavuşturmaktır. H. arenarium'un metanol ekstraktı, çeşitli fenolik bileşenleri tanımlamak ve miktarlarını belirlemek üzere Sıvı Kromatografi-Tandem Kütle Spektrometrisi (LC-MS/MS) kullanılarak analiz edilmiştir. Fenolik profil, luteolin (744.57 mg 100-1 g-1), kuersetin (113.13 mg 100-1 g-1) ve naringenin (229.60 mg 100-1 g-1) gibi yüksek konsantrasyonlarda bileşenler içerirken, diğer bileşenler tespit sınırının altındadır. Antioksidan kapasite, DPPH, ABTS, CUPRAC ve FRAP testleri kullanılarak değerlendirilmiş ve standart antioksidanlar olan BHA, BHT ve Trolox ile karşılaştırıldığında orta derecede aktivite gözlenmiştir. Metanol ekstraktı, DPPH ve ABTS radikal temizleme aktiviteleri sırasıyla %19.14 ve %26.91, FRAP ve CUPRAC absorbans değerleri ise sırasıyla 0.441 ve 0.653 olarak belirlenmiştir. Bu bulgular, H. arenarium'un doğal antioksidan kaynağı olarak potansiyelini vurgulamakta ve özellikle oksidatif stresle ilgili koşullarla mücadelede terapötik uygulamalarını optimize etmek için gelecekteki araştırmaların yolunu açmaktadır.

Anahtar Kelimeler

Phenolic Profile and Antioxidant Capacity of Helichrysum arenarium Extracts: A Comprehensive LC-MS/MS and Antioxidant Analysis

Abstract

Helichrysum arenarium, commonly known as the immortal or everlasting flower, is a member of the Asteraceae family celebrated for its potential medicinal properties. This study aims to elucidate the phenolic profile and antioxidant properties of H. arenarium using advanced analytical techniques. Methanol extract of H. arenarium was analyzed using Liquid Chromatography coupled with Tandem Mass Spectrometry (LC-MS/MS) to identify and quantify various phenolic compounds. The phenolic profile revealed high concentrations of luteolin (744.57 mg 100-1 g-1), quercetin (113.13 mg 100-1 g-1), and naringenin (229.60 mg 100-1 g-1), while other compounds were below the limit of quantification. The antioxidant capacity was evaluated using DPPH, ABTS, CUPRAC, and FRAP assays, showing moderate activity compared to standard antioxidants such as BHA, BHT, and Trolox. The methanol extract exhibited DPPH and ABTS radical scavenging activities of 19.14% and 26.91%, respectively, with FRAP and CUPRAC absorbance values of 0.441 and 0.653. These findings highlight the potential of H. arenarium as a source of natural antioxidants and pave the way for future research to optimize its therapeutic applications, especially in combating oxidative stress-related conditions.

Keywords

Ethical Statement

This study doesn't require an ethics committee decision.

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Details

Primary Language

English

Subjects

Plant Biochemistry , Medicinal and Aromatic Plants

Journal Section

Research Article

Authors

Yılmaz Uğur ^*
0000-0002-9040-4249
Türkiye

Abdussamat Güzel
0000-0001-7810-4510
Türkiye

Early Pub Date

January 30, 2025

Publication Date

February 12, 2025

Submission Date

September 9, 2024

Acceptance Date

December 25, 2024

Published in Issue

Year 2025 Volume: 28 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1545680

IZ

https://izlik.org/JA67EJ28GN

Cite

RIS / Bibtex

APA

Uğur, Y., & Güzel, A. (2025). Phenolic Profile and Antioxidant Capacity of Helichrysum arenarium Extracts: A Comprehensive LC-MS/MS and Antioxidant Analysis. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(1), 1-8. https://doi.org/10.18016/ksutarimdoga.vi.1545680

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