Genotypic Responses of Some Besni Pepper (Capsicum annuum L.) Genotypes to Anther Culture

Mirac Şahin; Halit Yetişir; Hasan Pinar; Alim Aydın

doi:10.18016/ksutarimdoga.vi.1555480

TR EN

Bazı Besni Biberi (Capsicum annuum L.) Genotiplerinin Anter Kültürüne Karşı Tepkileri

Öz

Bu çalışmada, Türkiye'nin yerel çeşitlerinden biri olan Besni biberinin (Capsium annuum L.) anter kültürüne cevabı belirlenmiştir. Toplam 26 Besni biber genotipi ve 3 kontrol çeşidi anter kültürüne cevapları açısından incelenmiştir. Her biber genotipinden 150 anter in vitro koşullarda kültüre alınmıştır. Anter kültürüne cevap açısından genotipler arasında anlamlı bir fark (%0,0-%45,3) bulunmuştur. En yüksek embriyo oluşum oranı %45,3 (63 embriyo) ile B11 genotipinde, en yüksek bitkiye dönüşüm oranı ise 30 bitki (%68) ile B15 genotipinde bulunmuştur. Kontrol çeşitleriyle karşılaştırıldığında, Besni biber genotipleri anlamlı olarak daha fazla embriyo üretmiştir. Kullanılan 26 genotipten ikisi hariç tüm genotipler daha fazla veya daha az (1-68) embriyo üretmiştir. B4, B10, B11, B12, B15 ve G6 genotipleri %25'ten fazla embriyo üretmiş ve kontrol ve diğer genotiplerden ayrılmıştır. Besni ve Gölbaşı ilçelerinden toplanan genotiplerin ortalama embriyo oluşum oranı sırasıyla %13 ve %7 iken, kontrol çeşitlerinin embriyo oluşum oranı sadece %0,2'dir. Besni biber popülasyonunun androgenetik haploide oldukça duyarlı olduğu sonucuna varılmıştır. Yüksek embriyo oluşturan B4, B10, B11, B12, B15 ve G6 gibi oldukça duyarlı genotipler yeni ıslah hatlarının geliştirilmesinde ve anter kültürünün genetiğinin araştırıldığı çalışmalarda kullanılma potansiyeline sahiptir.

Anahtar Kelimeler

Genotypic Responses of Some Besni Pepper (Capsicum annuum L.) Genotypes to Anther Culture

Abstract

In this study, the response of Besni pepper (Capsicum annuum L), one of the local varieties of Turkey, to anther culture was determined. A total of 26 Besni pepper genotypes and 3 control cultivars were examined for their response to anther culture. One hundred and fifty anthers from each pepper genotype were cultured under in vitro conditions. A significant difference (0.0%-45.3%) was found among the genotypes in terms of response to anther culture. The highest embryo formation rate was found in genotype B11 with 45.3% (63 embryos) and the highest transformation rate to plant was found in genotype B15 with 30 plants (68%). Compared to control varieties, Besni pepper genotypes produced significantly more embryos. All genotypes except two out of 26 genotypes used produced more or less (1-68) embryos. B4, B10, B11, B12, B15, and G6 genotypes produced more than 25% of embryos and were separated from the control and other genotypes. The average embryo formation rate of the genotypes collected from Besni and Gölbaşı districts was 13% and 7%, respectively, while the embryo formation rate of the control varieties was only 0.2%. It was concluded that the Besni pepper population was highly responsive to androgenetic haploid. The highly responsive genotypes that form high embryos such as B4, B10, B11, B12, B15, and G6 have the potential to be used in developing new breeding lines and in studies investigating the genetics of anther culture.

Keywords

Supporting Institution

Erciyes University Scientific Research Projects Coordinating Office

Project Number

FYL-2021-10960

Ethical Statement

Makale yazarları, bu makalede sunulan verilerin, bilgilerin ve dokümanların akademik ve etik kurallar çerçevesinde elde edildiğini, tüm bilgi, belge, değerlendirme ve sonuçlarının bilimsel etik ve ahlak kurallarına uygun olarak sunulduğunu beyan ederler

Thanks

The authors would like to thank Erciyes University Scientific Research Coordination Unit for its financial support and Erciyes University Agricultural Research and Application Center for the space and material support it provided.

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Details

Primary Language

English

Subjects

Plant Biotechnology

Journal Section

Research Article

Authors

Mirac Şahin
0000-0002-7699-6380
Türkiye

Halit Yetişir ^*
0000-0001-6955-9513
Türkiye

Hasan Pinar
0000-0002-0811-8228
Türkiye

Alim Aydın
0000-0002-9424-5556
Türkiye

Early Pub Date

January 30, 2025

Publication Date

February 12, 2025

Submission Date

September 25, 2024

Acceptance Date

January 3, 2025

Published in Issue

Year 2025 Volume: 28 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1555480

IZ

https://izlik.org/JA36BP53UD

Cite

RIS / Bibtex

APA

Şahin, M., Yetişir, H., Pinar, H., & Aydın, A. (2025). Genotypic Responses of Some Besni Pepper (Capsicum annuum L.) Genotypes to Anther Culture. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(1), 114-131. https://doi.org/10.18016/ksutarimdoga.vi.1555480

Cited By

Development of Phytophthora capsici–resistant Kahramanmaraş pepper lines using marker-assisted selection and anther culture

International Journal of Agriculture, Environment and Food Sciences

https://doi.org/10.31015/jaefs.2026.1.17