In Vivo and in Silico Evaluation of the Effect of p-Acetamide and MPAEMA on the Model Organism Galleria Mellonella (Lepidoptera: Pyralidae)

Nevin Çankaya; Serap Yalcin; Fahriye Ercan

doi:10.18016/ksutarimdoga.vi.1591091

EN TR

In Vivo and in Silico Evaluation of the Effect of p-Acetamide and MPAEMA on the Model Organism Galleria Mellonella (Lepidoptera: Pyralidae)

Abstract

In this study, 2-chloro-N-(4-methoxyphenyl)acetamide (p-acetamide) and 2-(4-methoxyphenylamino)-2-oxoethyl methacrylate (MPAEMA) were resynthesized to evaluate their effect on the agricultural pest Galleria mellonella. The toxicities of p-acetamide and MPAEMA against the larval stage of G. mellonella were evaluated concurrently. The results indicate that p-acetamide has a lethal effect on insect larvae at lower doses. LC50 doses of p-acetamide and MPAEMA were 873,572 and 687,355 uM, respectively. These values represent the concentrations of the substances at which 50% of the larvae exposed to them are expected to die. The molecular docking interactions of p-acetamide and MPAEMA with the proteins superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) were analyzed. The binding energy between MPAEMA and glutathione peroxidase was determined to be -6.8 kcal/mol. This suggests that MPAEMA may have an inhibitory effect on glutathione peroxidase and could be further investigated for developing pesticides that target this enzyme.

Keywords

p-asetamid ve MPAEMA'nın model organizma Galleria mellonella (Lepidoptera: Pyralidae) üzerindeki etkisinin in vivo ve in silico değerlendirilmesi

Öz

Bu çalışmada 2-kloro-N-(4-metoksifenil)asetamid (p-asetamid) ve 2-(4-metoksifenilamino)-2-oksoetil metakrilat (MPAEMA), tarımsal zararlı Galleria mellonella üzerindeki etkilerini değerlendirmek amacıyla yeniden sentezlenmiştir. p-asetamid ve MPAEMA'nın G. mellonella'nın larva evresine karşı toksisiteleri eş zamanlı olarak değerlendirilmiştir. Sonuçlar, p-asetamidin daha düşük dozlarda böcek larvaları üzerinde öldürücü etkiye sahip olduğunu göstermektedir. p-asetamid ve MPAEMA'nın LC50 dozları sırasıyla 873.572 ve 687.355 uM'dir. Bu değerler, bu maddelere maruz kalan larvaların %50'sinin ölmesinin beklendiği madde konsantrasyonlarını temsil etmektir. p-asetamid ve MPAEMA'nın süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx) ve glutatyon-S-transferaz (GST) proteinleriyle moleküler yerleştirme etkileşimleri analiz edildi. MPAEMA ve glutatyon peroksidaz arasındaki bağlanma enerjisi -6,8 kcal/mol olarak belirlendi. Bu, MPAEMA'nın glutatyon peroksidaz üzerinde inhibitör bir etkiye sahip olabileceğini ve bu enzimi hedef alan pestisitlerin geliştirilmesi için daha fazla araştırılabileceğini düşündürmektedir.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Plant Cell and Molecular Biology

Journal Section

Research Article

Authors

Nevin Çankaya ^*
0000-0002-6079-4987
Türkiye

Serap Yalcin
0000-0002-9584-266X
Türkiye

Fahriye Ercan
0000-0002-0111-8460
Türkiye

Early Pub Date

May 1, 2025

Publication Date

June 9, 2025

Submission Date

December 2, 2024

Acceptance Date

April 10, 2025

Published in Issue

Year 2025 Volume: 28 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1591091

IZ

https://izlik.org/JA92TX85NY

Cite

RIS / Bibtex

APA

Çankaya, N., Yalcin, S., & Ercan, F. (2025). In Vivo and in Silico Evaluation of the Effect of p-Acetamide and MPAEMA on the Model Organism Galleria Mellonella (Lepidoptera: Pyralidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(3), 625-635. https://doi.org/10.18016/ksutarimdoga.vi.1591091

Cited By

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Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1729010