In Vivo and in Silico Evaluation of the Effect of p-Acetamide and MPAEMA on the Model Organism Galleria Mellonella (Lepidoptera: Pyralidae)

Nevin Çankaya; Serap Yalcin; Fahriye Ercan

doi:10.18016/ksutarimdoga.vi.1591091

EN TR

In Vivo and in Silico Evaluation of the Effect of p-Acetamide and MPAEMA on the Model Organism Galleria Mellonella (Lepidoptera: Pyralidae)

Abstract

In this study, 2-chloro-N-(4-methoxyphenyl)acetamide (p-acetamide) and 2-(4-methoxyphenylamino)-2-oxoethyl methacrylate (MPAEMA) were resynthesized to evaluate their effect on the agricultural pest Galleria mellonella. The toxicities of p-acetamide and MPAEMA against the larval stage of G. mellonella were evaluated concurrently. The results indicate that p-acetamide has a lethal effect on insect larvae at lower doses. LC50 doses of p-acetamide and MPAEMA were 873,572 and 687,355 uM, respectively. These values represent the concentrations of the substances at which 50% of the larvae exposed to them are expected to die. The molecular docking interactions of p-acetamide and MPAEMA with the proteins superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) were analyzed. The binding energy between MPAEMA and glutathione peroxidase was determined to be -6.8 kcal/mol. This suggests that MPAEMA may have an inhibitory effect on glutathione peroxidase and could be further investigated for developing pesticides that target this enzyme.

Keywords

p-asetamid ve MPAEMA'nın model organizma Galleria mellonella (Lepidoptera: Pyralidae) üzerindeki etkisinin in vivo ve in silico değerlendirilmesi

Öz

Bu çalışmada 2-kloro-N-(4-metoksifenil)asetamid (p-asetamid) ve 2-(4-metoksifenilamino)-2-oksoetil metakrilat (MPAEMA), tarımsal zararlı Galleria mellonella üzerindeki etkilerini değerlendirmek amacıyla yeniden sentezlenmiştir. p-asetamid ve MPAEMA'nın G. mellonella'nın larva evresine karşı toksisiteleri eş zamanlı olarak değerlendirilmiştir. Sonuçlar, p-asetamidin daha düşük dozlarda böcek larvaları üzerinde öldürücü etkiye sahip olduğunu göstermektedir. p-asetamid ve MPAEMA'nın LC50 dozları sırasıyla 873.572 ve 687.355 uM'dir. Bu değerler, bu maddelere maruz kalan larvaların %50'sinin ölmesinin beklendiği madde konsantrasyonlarını temsil etmektir. p-asetamid ve MPAEMA'nın süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx) ve glutatyon-S-transferaz (GST) proteinleriyle moleküler yerleştirme etkileşimleri analiz edildi. MPAEMA ve glutatyon peroksidaz arasındaki bağlanma enerjisi -6,8 kcal/mol olarak belirlendi. Bu, MPAEMA'nın glutatyon peroksidaz üzerinde inhibitör bir etkiye sahip olabileceğini ve bu enzimi hedef alan pestisitlerin geliştirilmesi için daha fazla araştırılabileceğini düşündürmektedir.

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Hücresi ve Moleküler Biyoloji

Bölüm

Araştırma Makalesi

Yazarlar

Nevin Çankaya ^*
0000-0002-6079-4987
Türkiye

Serap Yalcin
0000-0002-9584-266X
Türkiye

Fahriye Ercan
0000-0002-0111-8460
Türkiye

Erken Görünüm Tarihi

1 Mayıs 2025

Yayımlanma Tarihi

9 Haziran 2025

Gönderilme Tarihi

2 Aralık 2024

Kabul Tarihi

10 Nisan 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 28 Sayı: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1591091

IZ

https://izlik.org/JA92TX85NY

Kaynak Göster

RIS / Bibtex

APA

Çankaya, N., Yalcin, S., & Ercan, F. (2025). In Vivo and in Silico Evaluation of the Effect of p-Acetamide and MPAEMA on the Model Organism Galleria Mellonella (Lepidoptera: Pyralidae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(3), 625-635. https://doi.org/10.18016/ksutarimdoga.vi.1591091

Cited By

Vitamin D3'ün Galleria mellonella (Lepidoptera: Pyralidae) Larvaları Üzerinde Biyokimyasal ve Genotoksik Etkileri

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1729010

In Vivo and in Silico Evaluation of the Effect of p-Acetamide and MPAEMA on the Model Organism Galleria Mellonella (Lepidoptera: Pyralidae)

Abstract

Keywords

p-asetamid ve MPAEMA'nın model organizma Galleria mellonella (Lepidoptera: Pyralidae) üzerindeki etkisinin in vivo ve in silico değerlendirilmesi

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Vitamin D3'ün Galleria mellonella (Lepidoptera: Pyralidae) Larvaları Üzerinde Biyokimyasal ve Genotoksik Etkileri

e-ISSN: 2619-9149