Determination of Shoot Regeneration Protocol in Aronia (Aronia melanocarpa (Michx.) Elliott) with New Generation Temporary Immersion Bioreactor System

Merve Karakoyun Mutluay; Şeyma Arıkan

doi:10.18016/ksutarimdoga.vi.1644524

TR EN

Aronya (Aronia melanocarpa (Michx.) Elliott)’ da Yeni Nesil Geçici Daldırma Biyoreaktör Sistemi ile Sürgün Rejenerasyon Protokolünün Belirlenmesi

Öz

Aronya (Aronia melanocarpa), Rosaceae familyasına ait endüstriyel açıdan önemli bir türdür. Son yıllarda meyveler arasında önemi artmakta olup, sağlık üzerindeki etkileri nedeniyle “süper meyve” olarak adlandırılmaktadır. Bu çalışmanın amacı, ‘Viking’ aronya çeşidinin in vitro çoğaltımı, yeni nesil olarak bilinen PlantForm biyoreaktör sistemi ile geleneksel bir doku kültürü yaklaşımı olan katı kültür çoğaltım yöntemiyle karşılaştırmaktır. Katı kültür mikroçoğaltımı ve PlantForm biyoreaktör sistemi denemelerinde MS besin ortamı ve sitokinin (BAP, KIN) kaynakları (1.0, 2.0 ve 4.0 mgL-1) kullanılmıştır. Her alt kültür dönemi sonunda her iki sistemde yapılan mikroçoğaltım deneylerinde sürgün sayısı (adet/fide), bitki boyu (cm), bitki çapı (mm), yaprak sayısı (adet), bitkinin yaş ve kuru ağırlığı (g), köklenme oranı (%), kök sayısı (adet), kök uzunluğu (mm), bitki boyu (cm) ve kökün yaş ve kuru ağırlığı (g) gibi veriler incelenmiştir. Sürgün canlılığında her iki ortamda da %100 canlılık oranları ile KIN kullanılan ortamlar daha iyi sonuç vermiştir. PlantForm sistemi, bitki boyu ile yaş ve kuru ağırlık açısından katı kültür sistemine kıyasla daha iyi sonuçlar vermiştir. Nitekim bitki boyunda 4.12cm ile en fazla değer MS+4.0 mgL-1 KIN+0.01 mgL-1 IAA ortamından elde edilmiştir. Bitki yaş ve kuru ağırlığında da en iyi değerler PlantForm sisteminde MS+2.0 mgL-1 BAP+0.01 mgL-1 IAA ortamında tespit edilmiştir. Bu çalışma, mikroçoğaltım açısından PlantForm biyoreaktör sisteminin katı kültür ortamına kıyasla daha etkili olduğunu ortaya koymuştur.

Anahtar Kelimeler

Determination of Shoot Regeneration Protocol in Aronia (Aronia melanocarpa (Michx.) Elliott) with New Generation Temporary Immersion Bioreactor System

Abstract

Aronia (Aronia melanocarpa) is an industrially important species belonging to the Rosaceae family. Among the berries, its importance has been increasing in recent years, and it is called a “super fruit” due to its effects on health. The purpose of this study was to compare the in vitro propagation of the ‘Viking’ aronia cultivar via the PlantForm bioreactor system, known as the new generation, with that via the solid culture propagation method, a traditional tissue culture approach. MS nutrient medium and cytokinin (BAP, KIN) sources (1.0, 2.0, and 4.0 mgL-1) were used in solid culture micropropagation and PlantForm bioreactor system trials. At the end of each subculture, the number of shoots (pcs/plantlet), plant height (cm), plant diameter (mm), number of leaves (pcs), plant fresh and dry weight (g) data, rooting rate (%), root number (pcs), root length (mm), plant height (cm), and root fresh and dry weight (g) data were examined in the micropropagation experiments in both systems. In terms of shoot vitality, the media containing KIN gave better results with 100% vitality rates in both systems. The PlantForm system yielded better plant height and fresh and dry weight results than the solid culture system. In fact, the highest value of 4.12 cm in plant height was obtained from MS+4.0 mgL-1 KIN+0.01 mgL-1 IAA medium. The best values in plant fresh and dry weight were determined in MS+2.0 mgL-1 BAP+0.01 mgL-1 IAA medium in the PlantForm system. In this study, the PlantForm bioreactor system was more effective for micropropagation than solid culture media.

Keywords

Supporting Institution

Selçuk Üniversitesi Bilimsel Projeler Koordinatörlüğü

Project Number

22401067

References

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Details

Primary Language

English

Subjects

Pomology and Treatment , Horticultural Production (Other)

Journal Section

Research Article

Authors

Merve Karakoyun Mutluay
0000-0001-7438-4738
Türkiye

Şeyma Arıkan ^*
0000-0002-4328-0263
Türkiye

Early Pub Date

January 21, 2026

Publication Date

January 21, 2026

Submission Date

February 24, 2025

Acceptance Date

September 5, 2025

Published in Issue

Year 1970 Volume: 29 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1644524

IZ

https://izlik.org/JA86UR38MM

Cite

RIS / Bibtex

APA

Karakoyun Mutluay, M., & Arıkan, Ş. (2026). Determination of Shoot Regeneration Protocol in Aronia (Aronia melanocarpa (Michx.) Elliott) with New Generation Temporary Immersion Bioreactor System. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 29(2), 322-334. https://doi.org/10.18016/ksutarimdoga.vi.1644524