Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress

Ramazan Keleş; Mustafa Küçüködük

doi:10.18016/ksutarimdoga.vi.1651818

EN TR

Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress

Abstract

Climate change, driven by human activity, has accelerated global warming, leading to heightened water scarcity and drought globally. In regions like Turkey and the Mediterranean basin, changing climate patterns threaten key sectors such as agriculture, where wheat is a staple crop. This study investigated the osmotic stress tolerance of two bread wheat genotypes, Bezostaya-1 and Karahan-99, by analyzing their superoxide dismutase (SOD) enzymes and isoenzymes under hydroponic conditions. Plants were exposed to osmotic stress at the vegetative and generative growth stages, alongside a control group. SOD enzyme activities and isozyme profiles were analyzed. The results showed that both genotypes produced different activities of Mn-SOD, Cu/Zn-SOD, and Fe-SOD isoenzymes under osmotic stress. Cu/Zn-SOD and Fe-SOD demonstrated peak activity, highlighting their vital role in mitigating oxidative damage. Total SOD activity increased significantly, especially during the generative stage, highlighting the importance of antioxidant defense mechanisms during critical growth phases under osmotic stress. Overall, the study highlights the importance of understanding how wheat genotypes respond biochemically to osmotic stress, particularly in the context of changing climate conditions. The results provide valuable information for the development of resistant wheat genotypes.

Keywords

Osmotik Stres Altındaki İki Ekmeklik Buğday Genotiplerinde Süperoksit Dismutaz ve İzozim Analizi

Abstract

İnsan faaliyetlerinin yol açtığı iklim değişikliği, küresel ısınmayı hızlandırarak küresel ölçekte su kıtlığı ve kuraklığın artmasına neden olmuştur. Türkiye ve Akdeniz havzası gibi bölgelerde değişen iklim modelleri, buğdayın temel bir ürün olduğu tarım gibi kilit sektörleri tehdit etmektedir. Bu çalışmada, Bezostaya-1 ve Karahan-99 adlı iki ekmeklik buğday genotipinin ozmotik stres toleransı, hidroponik koşullar altında süperoksit dismutaz (SOD) enzimleri ve izoenzimleri analiz edilerek araştırılmıştır. Bitkiler, bir kontrol grubu ile birlikte vejetatif ve generatif büyüme aşamalarında ozmotik strese maruz bırakılmıştır. SOD enzim aktiviteleri ve izozim profilleri analiz edilmiştir. Sonuçlar, her iki genotipin ozmotik stres altında farklı Mn-SOD, Cu/Zn-SOD ve Fe-SOD izoenzim aktiviteleri ürettiğini göstermiştir. Cu/Zn-SOD ve Fe-SOD en yüksek aktiviteyi göstererek oksidatif hasarın azaltılmasındaki hayati rollerini vurgulamışlardır. Toplam SOD aktivitesi, özellikle generatif aşamada önemli ölçüde artarak, ozmotik stres altındaki kritik büyüme aşamalarında antioksidan savunma mekanizmalarının önemini vurgulamıştır. Genel olarak çalışma, özellikle değişen iklim koşulları bağlamında buğday genotiplerinin ozmotik strese biyokimyasal olarak nasıl tepki verdiğini anlamanın önemini vurgulamaktadır. Sonuçlar, dayanıklı buğday genotiplerinin geliştirilmesi için değerli bilgiler sağlamaktadır.

Keywords

Supporting Institution

Selcuk University Scientific Research Projects Coordination Office

Project Number

20211010

Ethical Statement

Superoxide Dismutase and Isozyme Analysis in Some Bread Wheat Genotype Under Osmotic Stress isimli çalışmanın, etik kurul izni gerektirmeyen çalışmalar arasında yer aldığını beyan ederim

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Details

Primary Language

English

Subjects

Plant Biochemistry

Journal Section

Research Article

Authors

Ramazan Keleş ^*
0000-0003-2872-7183
Türkiye

Mustafa Küçüködük
0000-0001-6290-9007
Türkiye

Early Pub Date

June 10, 2025

Publication Date

July 4, 2025

Submission Date

March 5, 2025

Acceptance Date

May 29, 2025

Published in Issue

Year 2025 Volume: 28 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1651818

IZ

https://izlik.org/JA76HU44NX

Cite

RIS / Bibtex

APA

Keleş, R., & Küçüködük, M. (2025). Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 893-902. https://doi.org/10.18016/ksutarimdoga.vi.1651818

AMA

1.Keleş R, Küçüködük M. Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress. KSU J. Agric Nat. 2025;28(4):893-902. doi:10.18016/ksutarimdoga.vi.1651818

Chicago

Keleş, Ramazan, and Mustafa Küçüködük. 2025. “Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 28 (4): 893-902. https://doi.org/10.18016/ksutarimdoga.vi.1651818.

EndNote

Keleş R, Küçüködük M (July 1, 2025) Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28 4 893–902.

IEEE

[1]R. Keleş and M. Küçüködük, “Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress”, KSU J. Agric Nat., vol. 28, no. 4, pp. 893–902, July 2025, doi: 10.18016/ksutarimdoga.vi.1651818.

ISNAD

Keleş, Ramazan - Küçüködük, Mustafa. “Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28/4 (July 1, 2025): 893-902. https://doi.org/10.18016/ksutarimdoga.vi.1651818.

JAMA

1.Keleş R, Küçüködük M. Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress. KSU J. Agric Nat. 2025;28:893–902.

MLA

Keleş, Ramazan, and Mustafa Küçüködük. “Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 28, no. 4, July 2025, pp. 893-02, doi:10.18016/ksutarimdoga.vi.1651818.

Vancouver

1.Ramazan Keleş, Mustafa Küçüködük. Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress. KSU J. Agric Nat. 2025 Jul. 1;28(4):893-902. doi:10.18016/ksutarimdoga.vi.1651818

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Multivariate Assessment of Drought Stress Impacts on Oxidative Stress Indicators and Photosynthetic Pigment Profiles in Edible Pumpkin

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1782929