Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress

Ramazan Keleş; Mustafa Küçüködük

doi:10.18016/ksutarimdoga.vi.1651818

EN TR

Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress

Abstract

Climate change, driven by human activity, has accelerated global warming, leading to heightened water scarcity and drought globally. In regions like Turkey and the Mediterranean basin, changing climate patterns threaten key sectors such as agriculture, where wheat is a staple crop. This study investigated the osmotic stress tolerance of two bread wheat genotypes, Bezostaya-1 and Karahan-99, by analyzing their superoxide dismutase (SOD) enzymes and isoenzymes under hydroponic conditions. Plants were exposed to osmotic stress at the vegetative and generative growth stages, alongside a control group. SOD enzyme activities and isozyme profiles were analyzed. The results showed that both genotypes produced different activities of Mn-SOD, Cu/Zn-SOD, and Fe-SOD isoenzymes under osmotic stress. Cu/Zn-SOD and Fe-SOD demonstrated peak activity, highlighting their vital role in mitigating oxidative damage. Total SOD activity increased significantly, especially during the generative stage, highlighting the importance of antioxidant defense mechanisms during critical growth phases under osmotic stress. Overall, the study highlights the importance of understanding how wheat genotypes respond biochemically to osmotic stress, particularly in the context of changing climate conditions. The results provide valuable information for the development of resistant wheat genotypes.

Keywords

Osmotik Stres Altındaki İki Ekmeklik Buğday Genotiplerinde Süperoksit Dismutaz ve İzozim Analizi

Öz

İnsan faaliyetlerinin yol açtığı iklim değişikliği, küresel ısınmayı hızlandırarak küresel ölçekte su kıtlığı ve kuraklığın artmasına neden olmuştur. Türkiye ve Akdeniz havzası gibi bölgelerde değişen iklim modelleri, buğdayın temel bir ürün olduğu tarım gibi kilit sektörleri tehdit etmektedir. Bu çalışmada, Bezostaya-1 ve Karahan-99 adlı iki ekmeklik buğday genotipinin ozmotik stres toleransı, hidroponik koşullar altında süperoksit dismutaz (SOD) enzimleri ve izoenzimleri analiz edilerek araştırılmıştır. Bitkiler, bir kontrol grubu ile birlikte vejetatif ve generatif büyüme aşamalarında ozmotik strese maruz bırakılmıştır. SOD enzim aktiviteleri ve izozim profilleri analiz edilmiştir. Sonuçlar, her iki genotipin ozmotik stres altında farklı Mn-SOD, Cu/Zn-SOD ve Fe-SOD izoenzim aktiviteleri ürettiğini göstermiştir. Cu/Zn-SOD ve Fe-SOD en yüksek aktiviteyi göstererek oksidatif hasarın azaltılmasındaki hayati rollerini vurgulamışlardır. Toplam SOD aktivitesi, özellikle generatif aşamada önemli ölçüde artarak, ozmotik stres altındaki kritik büyüme aşamalarında antioksidan savunma mekanizmalarının önemini vurgulamıştır. Genel olarak çalışma, özellikle değişen iklim koşulları bağlamında buğday genotiplerinin ozmotik strese biyokimyasal olarak nasıl tepki verdiğini anlamanın önemini vurgulamaktadır. Sonuçlar, dayanıklı buğday genotiplerinin geliştirilmesi için değerli bilgiler sağlamaktadır.

Anahtar Kelimeler

Destekleyen Kurum

Selcuk University Scientific Research Projects Coordination Office

Proje Numarası

20211010

Etik Beyan

Superoxide Dismutase and Isozyme Analysis in Some Bread Wheat Genotype Under Osmotic Stress isimli çalışmanın, etik kurul izni gerektirmeyen çalışmalar arasında yer aldığını beyan ederim

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Biyokimyası

Bölüm

Araştırma Makalesi

Yazarlar

Ramazan Keleş ^*
0000-0003-2872-7183
Türkiye

Mustafa Küçüködük
0000-0001-6290-9007
Türkiye

Erken Görünüm Tarihi

10 Haziran 2025

Yayımlanma Tarihi

4 Temmuz 2025

Gönderilme Tarihi

5 Mart 2025

Kabul Tarihi

29 Mayıs 2025

Yayımlandığı Sayı

Yıl 2025 Cilt: 28 Sayı: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1651818

IZ

https://izlik.org/JA76HU44NX

Kaynak Göster

RIS / Bibtex

APA

Keleş, R., & Küçüködük, M. (2025). Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(4), 893-902. https://doi.org/10.18016/ksutarimdoga.vi.1651818

Cited By

Multivariate Assessment of Drought Stress Impacts on Oxidative Stress Indicators and Photosynthetic Pigment Profiles in Edible Pumpkin

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1782929

Superoxide Dismutase and Isozyme Analysis in Two Bread Wheat Genotypes Under Osmotic Stress

Abstract

Keywords

Osmotik Stres Altındaki İki Ekmeklik Buğday Genotiplerinde Süperoksit Dismutaz ve İzozim Analizi

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Etik Beyan

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

Multivariate Assessment of Drought Stress Impacts on Oxidative Stress Indicators and Photosynthetic Pigment Profiles in Edible Pumpkin

e-ISSN: 2619-9149