Assessment of microbiological quality of agricultural surface water sources in Muş plain

Tuba Karakoyun; Didem Taşdelen; Sedat Bozarı; Murad Aydın Şanda; Harun Önlü; Zeynal Topalcengiz

doi:10.18016/ksutarimdoga.vi.1662712

TR EN

Muş ovası tarımsal yüzey su kaynaklarının mikrobiyolojik kalitesinin değerlendirilmesi

Öz

Tarımsal sular, tarlalardaki mahsül kontaminasyonunun bir kaynağı olabilir. Bu çalışmanın amacı Muş ovasındaki tarımsal amaçlarla kullanılan yüzey su kaynaklarının mikrobiyolojik kalitesini belirlemektir. Tarım sezonu boyunca iki gölet ve iki akarsu olmak üzere dört farklı yüzey su kaynağından su örnekleri alınmıştır. Toplam koliform ve fekal koliform popülasyonları 100 mL su örneğinde En Muhtemel Sayı (MPN 100 mL-1) ile sayılmıştır. Jenerik Escherichia coli popülasyonları 100 mL su örneklerinde MPN ve CFU olarak belirlenmiştir. Su örneklerinden Jenerik E. coli izolasyonları yapılmış ve izolatların antibiyotik duyarlılık testi sonrası genetik akrabalıkları RAPD PCR ile belirlenmiştir. Salmonella varlığı da 100 mL su örneklerinde, olası kolonilerin izolasyonundan sonra InvA geninin doğrulanmasıyla belirlenmiştir. Toplam koliform popülasyonu > 2,96 log MPN 100 mL-1'ye ulaşmıştır. Tüm yüzey suyu kaynaklarındaki E. coli popülasyonu 0 ila >2,96 log MPN 100 mL-1 ve 0 ila 2,78 log CFU 100 mL-1 arasında değişmektedir. Salmonella varlığı iki kaynakta toplam üç su örneğinde (%5) pozitif bulunmuştur. Bütün E. coli izolatları rifampisin (%100), eritromisin (%100) ve vankomisine (%97,05) dirençli bulunmuştur. Akarsulardan izole edilen jenerik E. coli izolatları RAPD filogenetik analizine göre benzer kümelerde bir araya gelmemiştir. Bu çalışmanın sonuçları, Muş Ovası'ndaki tarımsal yüzey su kaynaklarının mahsülle teması sonucunda kontaminasyon riski taşıdığını göstermektedir.

Anahtar Kelimeler

Assessment of microbiological quality of agricultural surface water sources in Muş plain

Abstract

Agricultural waters may be a source of produce contamination in the fields. The purpose of this study was to determine microbiological quality of agricultural surface water sources in Muş plain. Water samples were examined for four different agricultural surface water resources including two ponds and two streams during the agricultural season. The population of total coliform and fecal coliform were enumerated with Most Probable Number in 100 mL water samples (MPN 100 mL). Generic Escherichia coli populations were determined as MPN and CFU isolates in 100 mL water samples. Generic E. coli isolates were recovered for antibiotic susceptibility test and genetic relationships analyses with RAPD PCR. The presence of Salmonella was also determined in 100 mL water samples with confirmation of InvA gene presence after isolation of presumptive colonies. Total coliform population reached >2.96 log MPN 100 mL-1. The E. coli population in all surface water sources ranged from 0 to >2.96 log MPN 100 mL-1 and 0 to 2.78 log CFU 100 mL-1. The presence of Salmonella was found to be positive in a total of three water samples in two sources (5%). E. coli isolates were found to be resistant to rifampicin (100%), erythromycin (100%), and vancomycin (97.05%). Generic E. coli populations isolated from streams were not grouped together in similar clusters. Results of this study indicate that agricultural surface water sources in Muş Plain may contain could be a contamination source when in contact with the produce.

Keywords

Supporting Institution

Muş Alparslan University- Scientific Research Coordination Unit under project number:

Project Number

BAP-21-TBMYO-4901-05 ve BAP-20-FEF-4902-05

Ethical Statement

Authors have declared no conflict of interest.

Thanks

This study was supported by Muş Alparslan University- Scientific Research Coordination Unit under project numbers: BAP-21-TBMYO-4901-05 ve BAP-20-FEF-4902-05.

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Details

Primary Language

English

Subjects

Microbial Ecology , Microbiology (Other)

Journal Section

Research Article

Authors

Tuba Karakoyun
0000-0003-3984-9063
Türkiye

Didem Taşdelen
0000-0002-7875-6428
Türkiye

Sedat Bozarı
0000-0001-5265-2236
Türkiye

Murad Aydın Şanda
0000-0001-8843-4361
Türkiye

Harun Önlü ^*
0000-0003-3660-9267
Türkiye

Zeynal Topalcengiz
0000-0002-2113-7319
Türkiye

Early Pub Date

August 14, 2025

Publication Date

October 20, 2025

Submission Date

March 21, 2025

Acceptance Date

July 11, 2025

Published in Issue

Year 2025 Volume: 28 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1662712

IZ

https://izlik.org/JA88MT88ZM

Cite

RIS / Bibtex

APA

Karakoyun, T., Taşdelen, D., Bozarı, S., Şanda, M. A., Önlü, H., & Topalcengiz, Z. (2025). Assessment of microbiological quality of agricultural surface water sources in Muş plain. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(6), 1492-1505. https://doi.org/10.18016/ksutarimdoga.vi.1662712