Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria monocytogenes

Zehra Tuğçe Toprak; Pınar Şanlıbaba; Sencer Buzrul

doi:10.18016/ksutarimdoga.vi.1701368

EN TR

Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria monocytogenes

Abstract

The aim of this study was to investigate the effect of pediocin on Listeria monocytogenes under optimal temperature conditions and to model the bacterial growth and survival dynamics in the presence and absence of pediocin. The susceptibility of 28 L. monocytogenes strains to pediocin was evaluated using Minimum Inhibitory Concentration (MIC) tests. To determine the antimicrobial efficacy of pediocin, solutions at concentrations of 7 µg/mL and 12 µg/mL were applied to the strains, including the reference strain, and the inhibitory effect on bacterial growth was assessed at 35 °C. Model parameters were estimated using R-BioXL software, with model performance supported by R², adjusted R², and RMSE values. The results showed that the L. monocytogenes 287-1P strain exhibited higher resistance to pediocin compared to other strains. Although pediocin reduced the growth rate and delayed bacterial proliferation, its effect as a sole treatment was limited. Therefore, it is recommended that pediocin be used in combination with other preservative strategies within a hurdle technology framework. The findings indicate that pediocin has potential as a bioprotective agent for controlling L. monocytogenes in food products, contributing to enhanced microbial safety and public health protection. Furthermore, modeling the effect of pediocin provides a valuable tool for evaluating the growth and survival dynamics of this pathogen.

Keywords

Pediyosin Uygulamasının Listeria monocytogenes’in Büyüme ve Yaşam Sürecine Etkisinin Modellenmesi

Abstract

Bu çalışmanın amacı, pediyosinin Listeria monocytogenes üzerindeki etkisini optimum sıcaklık koşullarında incelemek ve pediyosin varlığında ya da yokluğunda bakterinin büyüme ve hayatta kalma süreçlerini modellemektir. Çalışmada, 28 farklı L. monocytogenes suşunun pediyosine duyarlılığı Minimum İnhibitör Konsantrasyon (MIK) testleri ile değerlendirilmiştir. Pediyosinin antimikrobiyal etkisini belirlemek için suşlara 7 µg/mL ve 12 µg/mL konsantrasyonlarında pediyosin uygulanmış, 35 °C’de bakteriyel büyüme üzerindeki inhibitör etkisi incelenmiştir. Model parametreleri, R-BioXL yazılımı kullanılarak tahmin edilmiş ve model performansı R², düzeltilmiş R² (R²ₐdⱼ) ve RMSE değerleri ile desteklenmiştir. Sonuçlar, L. monocytogenes 287-1P suşunun pediyosin direncinin diğer suşlara göre daha yüksek olduğunu göstermiştir. Pediyosin, büyüme hızını düşürerek patojenin çoğalmasını geciktirse de, tek başına uygulandığında sınırlı bir kontrol sağlamaktadır. Bu nedenle, pediyosinin diğer koruyucu yöntemlerle birlikte, “hurdle teknolojisi” kapsamında kullanılması önerilmektedir. Elde edilen bulgular, pediyosinin gıda ürünlerinde L. monocytogenes kontrolünde biyokoruyucu ajan olarak potansiyel taşıdığını ve bu sayede mikrobiyal güvenliğin artırılarak halk sağlığının korunmasına katkı sağlanabileceğini ortaya koymaktadır. Ayrıca, pediyosin etkisinin modellenmesi, patojenin büyüme ve hayatta kalma dinamiklerinin değerlendirilmesinde etkili bir araç olarak önem taşımaktadır.

Keywords

Project Number

Ankara Üniversitesi Bilimsel Araştırma Projeleri (BAP), Proje Numarası: 21H0443002

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Details

Primary Language

English

Subjects

Microbiology (Other)

Journal Section

Research Article

Authors

Zehra Tuğçe Toprak
0000-0001-8353-4179
Türkiye

Pınar Şanlıbaba ^*
0000-0003-4638-6765
Türkiye

Sencer Buzrul
0000-0003-2272-3827
Türkiye

Early Pub Date

August 14, 2025

Publication Date

October 20, 2025

Submission Date

May 17, 2025

Acceptance Date

July 26, 2025

Published in Issue

Year 2025 Volume: 28 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1701368

IZ

https://izlik.org/JA94BE89RN

Cite

RIS / Bibtex

APA

Toprak, Z. T., Şanlıbaba, P., & Buzrul, S. (2025). Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria monocytogenes. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(6), 1405-1413. https://doi.org/10.18016/ksutarimdoga.vi.1701368

AMA

1.Toprak ZT, Şanlıbaba P, Buzrul S. Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria monocytogenes. KSU J. Agric Nat. 2025;28(6):1405-1413. doi:10.18016/ksutarimdoga.vi.1701368

Chicago

Toprak, Zehra Tuğçe, Pınar Şanlıbaba, and Sencer Buzrul. 2025. “Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria Monocytogenes”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 28 (6): 1405-13. https://doi.org/10.18016/ksutarimdoga.vi.1701368.

EndNote

Toprak ZT, Şanlıbaba P, Buzrul S (October 1, 2025) Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria monocytogenes. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28 6 1405–1413.

IEEE

[1]Z. T. Toprak, P. Şanlıbaba, and S. Buzrul, “Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria monocytogenes”, KSU J. Agric Nat., vol. 28, no. 6, pp. 1405–1413, Oct. 2025, doi: 10.18016/ksutarimdoga.vi.1701368.

ISNAD

Toprak, Zehra Tuğçe - Şanlıbaba, Pınar - Buzrul, Sencer. “Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria Monocytogenes”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 28/6 (October 1, 2025): 1405-1413. https://doi.org/10.18016/ksutarimdoga.vi.1701368.

JAMA

1.Toprak ZT, Şanlıbaba P, Buzrul S. Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria monocytogenes. KSU J. Agric Nat. 2025;28:1405–1413.

MLA

Toprak, Zehra Tuğçe, et al. “Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria Monocytogenes”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 28, no. 6, Oct. 2025, pp. 1405-13, doi:10.18016/ksutarimdoga.vi.1701368.

Vancouver

1.Zehra Tuğçe Toprak, Pınar Şanlıbaba, Sencer Buzrul. Modeling the Effect of Pediocin Application on the Growth and Survival of Listeria monocytogenes. KSU J. Agric Nat. 2025 Oct. 1;28(6):1405-13. doi:10.18016/ksutarimdoga.vi.1701368