Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS

Israt Jahan; Cumali Keskin; Ayşe Baran; Mehmet Fırat Baran; Sevgi İrtegün Kandemir; Deniz Evrim Kavak; Fatma Mungan Kılıç; Murat Kılıç

doi:10.18016/ksutarimdoga.vi.1792245

TR EN

Verbascum tenue Murb.'un Antikanser ve Anti-Göç Potansiyellerinin Araştırılması ve Biyoaktif Bileşiklerinin LC-ESI-MS/MS Kullanılarak Analizi

Abstract

Verbascum türleri (Scrophulariaceae), etnofarmakolojide çeşitli terapötik özellikleri ile bilinmektedir. Bu iddiaları bilimsel olarak araştırmak için, bu çalışma Verbascum tenue MURB., yapraklarının metanol özütünün (VTE) fitokimyasal bileşimini, in-vitro sitotoksisitesini ve anti-göç aktivitesini karakterize etmiştir. LC-ESI-MS/MS yoluyla yapılan fitokimyasal profilleme, vanilik asit, vanilin ve trans-ferulik asit ana bileşenleri olmak üzere dokuz bileşik tanımlamıştır. VTE'nin sitotoksik etkileri, SKOV-3 (yumurtalık adenokarsinomu), MCF-7 (meme karsinomu), U87 (glioblastoma) ve HDF (insan dermal fibroblastı) hücreleri de dahil olmak üzere bir dizi hücre hattı üzerinde, 15,625–500 µg/mL konsantrasyon aralığında MTT testi kullanılarak değerlendirilmiştir. VTE, SKOV-3 hücrelerinde 236,74 µg/mL, MCF-7 hücrelerinde 374,50 µg/mL ve HDF hücrelerinde 386,68 µg/mL IC₅₀ değerleriyle farklı sitotoksisite gösterdi. Yara iyileşme testi, SKOV-3 hücrelerinde yüksek toksisiteye (%59,21 canlılık) ve HDF hücrelerinde düşük toksisiteye (%91,06 canlılık) sahip olması nedeniyle seçilen 125 µg/mL VTE konsantrasyonu ile gerçekleştirildi. VTE, 12 saat boyunca kontrol grubunda gözlemlenen %40,36'ya kıyasla yara kapanmasını %25,86'ya kadar önemli ölçüde baskıladı. Hücre göçünün bu inhibisyonu, sitotoksik profiliyle birlikte, VTE'nin hem antikanser hem de anti-metastatik özelliklere sahip bir ajan olarak potansiyelini desteklemektedir.

Keywords

Ethical Statement

Etik kurul onayı gerektirecek bir çalışma olmadığını beyan ederiz.

Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS

Abstract

Verbascum species (Scrophulariaceae) are recognized in ethnopharmacology for their diverse therapeutic properties. To investigate these claims scientifically, this study characterized a methanol extract of Verbascum tenue Murb., leaves (VTE) for its phytochemical composition, in vitro cytotoxicity, and anti-migratory activity. Phytochemical profiling via LC-ESI-MS/MS identified nine compounds, with vanillic acid, vanillin, and trans-ferulic acid as the major constituents. The cytotoxic effects of VTE were evaluated on a panel of cell lines—including SKOV-3 (ovarian adenocarcinoma), MCF-7 (breast carcinoma), U87 (glioblastoma), and HDF (human dermal fibroblast) cells—using the MTT assay across a concentration range of 15.625–500 µg/mL. VTE exhibited differential cytotoxicity, with IC₅₀ values of 236.74 µg/mL in SKOV-3 cells, 374.5 µg/mL in MCF-7 cells, and 386.68 µg/mL in HDF cells. The wound healing assay was conducted with 125 µg/mL VTE, a concentration selected for its high toxicity on SKOV-3 (viability 59.21%) and low toxicity on HDF (viability 91.06%). VTE significantly suppressed wound closure to 25.86% relative to the 40.36% observed in controls over 12 hours. This inhibition of cell migration, in conjunction with its cytotoxic profile, supports the potential of VTE as an agent with both anticancer and anti-metastatic properties.

Keywords

Ethical Statement

We declare that there is no study requiring ethics committee approval.

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Details

Primary Language

English

Subjects

Plant Biochemistry

Journal Section

Research Article

Authors

Israt Jahan
0000-0003-4166-1617
Türkiye

Cumali Keskin ^*
0000-0003-3758-0654
Türkiye

Ayşe Baran
0000-0002-2317-0489
Türkiye

Mehmet Fırat Baran
0000-0001-8133-6670
Türkiye

Sevgi İrtegün Kandemir
0000-0001-6160-5626
Türkiye

Deniz Evrim Kavak
0000-0002-9681-4468
Türkiye

Fatma Mungan Kılıç
0000-0001-6858-3458
Türkiye

Murat Kılıç
0000-0002-6408-9660
Türkiye

Early Pub Date

September 20, 2026

Publication Date

-

Submission Date

September 27, 2025

Acceptance Date

February 22, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1792245

IZ

https://izlik.org/JA95CK85UH

Cite

RIS / Bibtex

APA

Jahan, I., Keskin, C., Baran, A., Baran, M. F., İrtegün Kandemir, S., Kavak, D. E., Mungan Kılıç, F., & Kılıç, M. (2026). Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication, 51-64. https://doi.org/10.18016/ksutarimdoga.vi.1792245

AMA

1.Jahan I, Keskin C, Baran A, et al. Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. KSU J. Agric Nat. 2026;(Advanced Online Publication):51-64. doi:10.18016/ksutarimdoga.vi.1792245

Chicago

Jahan, Israt, Cumali Keskin, Ayşe Baran, et al. 2026. “Investigation of Anticancer and Anti-Migration Potentials of Verbascum Tenue Murb. And Analysis of Its Bioactive Compounds Using LC–ESI–MS MS”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication: 51-64. https://doi.org/10.18016/ksutarimdoga.vi.1792245.

EndNote

Jahan I, Keskin C, Baran A, Baran MF, İrtegün Kandemir S, Kavak DE, Mungan Kılıç F, Kılıç M (September 1, 2026) Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication 51–64.

IEEE

[1]I. Jahan et al., “Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS”, KSU J. Agric Nat., no. Advanced Online Publication, pp. 51–64, Sept. 2026, doi: 10.18016/ksutarimdoga.vi.1792245.

ISNAD

Jahan, Israt - Keskin, Cumali - Baran, Ayşe - Baran, Mehmet Fırat - İrtegün Kandemir, Sevgi - Kavak, Deniz Evrim - Mungan Kılıç, Fatma - Kılıç, Murat. “Investigation of Anticancer and Anti-Migration Potentials of Verbascum Tenue Murb. And Analysis of Its Bioactive Compounds Using LC–ESI–MS MS”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (September 1, 2026): 51-64. https://doi.org/10.18016/ksutarimdoga.vi.1792245.

JAMA

1.Jahan I, Keskin C, Baran A, Baran MF, İrtegün Kandemir S, Kavak DE, Mungan Kılıç F, Kılıç M. Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. KSU J. Agric Nat. 2026;:51–64.

MLA

Jahan, Israt, et al. “Investigation of Anticancer and Anti-Migration Potentials of Verbascum Tenue Murb. And Analysis of Its Bioactive Compounds Using LC–ESI–MS MS”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Sept. 2026, pp. 51-64, doi:10.18016/ksutarimdoga.vi.1792245.

Vancouver

1.Israt Jahan, Cumali Keskin, Ayşe Baran, Mehmet Fırat Baran, Sevgi İrtegün Kandemir, Deniz Evrim Kavak, Fatma Mungan Kılıç, Murat Kılıç. Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. KSU J. Agric Nat. 2026 Sep. 1;(Advanced Online Publication):51-64. doi:10.18016/ksutarimdoga.vi.1792245