Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS

Israt Jahan; Cumali Keskin; Ayşe Baran; Mehmet Fırat Baran; Sevgi İrtegün Kandemir; Deniz Evrim Kavak; Fatma Mungan Kılıç; Murat Kılıç

doi:10.18016/ksutarimdoga.vi.1792245

TR EN

Verbascum tenue Murb.'un Antikanser ve Anti-Göç Potansiyellerinin Araştırılması ve Biyoaktif Bileşiklerinin LC-ESI-MS/MS Kullanılarak Analizi

Öz

Verbascum türleri (Scrophulariaceae), etnofarmakolojide çeşitli terapötik özellikleri ile bilinmektedir. Bu iddiaları bilimsel olarak araştırmak için, bu çalışma Verbascum tenue MURB., yapraklarının metanol özütünün (VTE) fitokimyasal bileşimini, in-vitro sitotoksisitesini ve anti-göç aktivitesini karakterize etmiştir. LC-ESI-MS/MS yoluyla yapılan fitokimyasal profilleme, vanilik asit, vanilin ve trans-ferulik asit ana bileşenleri olmak üzere dokuz bileşik tanımlamıştır. VTE'nin sitotoksik etkileri, SKOV-3 (yumurtalık adenokarsinomu), MCF-7 (meme karsinomu), U87 (glioblastoma) ve HDF (insan dermal fibroblastı) hücreleri de dahil olmak üzere bir dizi hücre hattı üzerinde, 15,625–500 µg/mL konsantrasyon aralığında MTT testi kullanılarak değerlendirilmiştir. VTE, SKOV-3 hücrelerinde 236,74 µg/mL, MCF-7 hücrelerinde 374,50 µg/mL ve HDF hücrelerinde 386,68 µg/mL IC₅₀ değerleriyle farklı sitotoksisite gösterdi. Yara iyileşme testi, SKOV-3 hücrelerinde yüksek toksisiteye (%59,21 canlılık) ve HDF hücrelerinde düşük toksisiteye (%91,06 canlılık) sahip olması nedeniyle seçilen 125 µg/mL VTE konsantrasyonu ile gerçekleştirildi. VTE, 12 saat boyunca kontrol grubunda gözlemlenen %40,36'ya kıyasla yara kapanmasını %25,86'ya kadar önemli ölçüde baskıladı. Hücre göçünün bu inhibisyonu, sitotoksik profiliyle birlikte, VTE'nin hem antikanser hem de anti-metastatik özelliklere sahip bir ajan olarak potansiyelini desteklemektedir.

Anahtar Kelimeler

Etik Beyan

Etik kurul onayı gerektirecek bir çalışma olmadığını beyan ederiz.

Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS

Öz

Verbascum species (Scrophulariaceae) are recognized in ethnopharmacology for their diverse therapeutic properties. To investigate these claims scientifically, this study characterized a methanol extract of Verbascum tenue Murb., leaves (VTE) for its phytochemical composition, in vitro cytotoxicity, and anti-migratory activity. Phytochemical profiling via LC-ESI-MS/MS identified nine compounds, with vanillic acid, vanillin, and trans-ferulic acid as the major constituents. The cytotoxic effects of VTE were evaluated on a panel of cell lines—including SKOV-3 (ovarian adenocarcinoma), MCF-7 (breast carcinoma), U87 (glioblastoma), and HDF (human dermal fibroblast) cells—using the MTT assay across a concentration range of 15.625–500 µg/mL. VTE exhibited differential cytotoxicity, with IC₅₀ values of 236.74 µg/mL in SKOV-3 cells, 374.5 µg/mL in MCF-7 cells, and 386.68 µg/mL in HDF cells. The wound healing assay was conducted with 125 µg/mL VTE, a concentration selected for its high toxicity on SKOV-3 (viability 59.21%) and low toxicity on HDF (viability 91.06%). VTE significantly suppressed wound closure to 25.86% relative to the 40.36% observed in controls over 12 hours. This inhibition of cell migration, in conjunction with its cytotoxic profile, supports the potential of VTE as an agent with both anticancer and anti-metastatic properties.

Anahtar Kelimeler

Etik Beyan

We declare that there is no study requiring ethics committee approval.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Biyokimyası

Bölüm

Araştırma Makalesi

Yazarlar

Israt Jahan
0000-0003-4166-1617
Türkiye

Cumali Keskin ^*
0000-0003-3758-0654
Türkiye

Ayşe Baran
0000-0002-2317-0489
Türkiye

Mehmet Fırat Baran
0000-0001-8133-6670
Türkiye

Sevgi İrtegün Kandemir
0000-0001-6160-5626
Türkiye

Deniz Evrim Kavak
0000-0002-9681-4468
Türkiye

Fatma Mungan Kılıç
0000-0001-6858-3458
Türkiye

Murat Kılıç
0000-0002-6408-9660
Türkiye

Erken Görünüm Tarihi

20 Eylül 2026

Yayımlanma Tarihi

-

Gönderilme Tarihi

27 Eylül 2025

Kabul Tarihi

22 Şubat 2026

Yayımlandığı Sayı

Yıl 2026 Sayı: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1792245

IZ

https://izlik.org/JA95CK85UH

Kaynak Göster

RIS / Bibtex

APA

Jahan, I., Keskin, C., Baran, A., Baran, M. F., İrtegün Kandemir, S., Kavak, D. E., Mungan Kılıç, F., & Kılıç, M. (2026). Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Advanced Online Publication, 51-64. https://doi.org/10.18016/ksutarimdoga.vi.1792245

AMA

1.Jahan I, Keskin C, Baran A, vd. Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2026;(Advanced Online Publication):51-64. doi:10.18016/ksutarimdoga.vi.1792245

Chicago

Jahan, Israt, Cumali Keskin, Ayşe Baran, vd. 2026. “Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication: 51-64. https://doi.org/10.18016/ksutarimdoga.vi.1792245.

EndNote

Jahan I, Keskin C, Baran A, Baran MF, İrtegün Kandemir S, Kavak DE, Mungan Kılıç F, Kılıç M (01 Eylül 2026) Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication 51–64.

IEEE

[1]I. Jahan vd., “Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS”, Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication, ss. 51–64, Eyl. 2026, doi: 10.18016/ksutarimdoga.vi.1792245.

ISNAD

Jahan, Israt - Keskin, Cumali - Baran, Ayşe - Baran, Mehmet Fırat - İrtegün Kandemir, Sevgi - Kavak, Deniz Evrim - Mungan Kılıç, Fatma - Kılıç, Murat. “Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (01 Eylül 2026): 51-64. https://doi.org/10.18016/ksutarimdoga.vi.1792245.

JAMA

1.Jahan I, Keskin C, Baran A, Baran MF, İrtegün Kandemir S, Kavak DE, Mungan Kılıç F, Kılıç M. Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2026;:51–64.

MLA

Jahan, Israt, vd. “Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication, Eylül 2026, ss. 51-64, doi:10.18016/ksutarimdoga.vi.1792245.

Vancouver

1.Israt Jahan, Cumali Keskin, Ayşe Baran, Mehmet Fırat Baran, Sevgi İrtegün Kandemir, Deniz Evrim Kavak, Fatma Mungan Kılıç, Murat Kılıç. Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 01 Eylül 2026;(Advanced Online Publication):51-64. doi:10.18016/ksutarimdoga.vi.1792245

Verbascum tenue Murb.'un Antikanser ve Anti-Göç Potansiyellerinin Araştırılması ve Biyoaktif Bileşiklerinin LC-ESI-MS/MS Kullanılarak Analizi

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Etik Beyan

Investigation of Anticancer and Anti-Migration Potentials of Verbascum tenue Murb. and Analysis of its Bioactive Compounds using LC–ESI–MS/MS

Öz

Anahtar Kelimeler

Etik Beyan

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