Determination of Chemical Composition and In Vitro Antimicrobial Activity of Thymus longicaulis subsp. longicaulis Plant Extracts and Essential Oil

Aysel Veyisoğlu; Medine Karabörk; Demet Tatar; Hünkar Avni Duyar

doi:10.18016/ksutarimdoga.vi.1829240

EN TR

Determination of Chemical Composition and In Vitro Antimicrobial Activity of Thymus longicaulis subsp. longicaulis Plant Extracts and Essential Oil

Abstract

Members of the Thymus genus are known worldwide as popular functional foods and folk medicines. The chemical composition and antimicrobial properties of essential oils obtained from aerial parts (flower, leaf and stem) of Thymus longicaulis subsp. longicaulis collected from Kaledibi village of Alucra district of Giresun and the antimicrobial properties of ethanol and methanol extracts were investigated. Twenty components representing 95.7% of the essential oils were identified. The main compounds in the essential oil of T. longicaulis subsp. longicaulis are: Thymol (37.96%), p-Cymene (22.17%), Carvacrol (11.75%), and L-Borneol (4.90%). The essential oil and extracts were screened for their antimicrobial activities against 7 bacterial and 2 fungal species using disk diffusion method. It was observed that the essential oil exhibited better antibacterial activity than ethanol and methanol extracts.

Keywords

Thymus longicaulis subsp. longicaulis Bitki Ekstraktlarının ve Uçucu Yağının Kimyasal Bileşiminin Belirlenmesi ve İn Vitro Antimikrobiyal Aktivitesi

Öz

Thymus cinsi üyeleri, dünya genelinde popüler fonksiyonel gıdalar ve halk ilaçları olarak bilinmektedir. Giresun’un Alucra ilçesine bağlı Kaledibi köyünden toplanan Thymus longicaulis subsp. longicaulis bitkisinin toprak üstü kısımlarından (çiçek, yaprak ve gövde) elde edilen uçucu yağların kimyasal bileşimi ve antimikrobiyal özellikleri ile etanol ve metanol ekstraktlarının antimikrobiyal özellikleri araştırılmıştır. Uçucu yağların %95,7’sini temsil eden yirmi bileşen tanımlanmıştır. T. longicaulis subsp. longicaulis uçucu yağının ana bileşenleri şunlardır: Timol (%37,96), p-Simen (%22,17), Karvakrol (%11,75) ve L-Borneol (%4,90). Uçucu yağ ve ekstraktların antimikrobiyal aktiviteleri, disk difüzyon yöntemi kullanılarak 7 bakteri ve 2 mantar türüne karşı test edilmiştir. Uçucu yağın, etanol ve metanol ekstraktlarına göre daha iyi antibakteriyel aktivite gösterdiği gözlemlenmiştir.

Anahtar Kelimeler

Supporting Institution

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK)

Project Number

1919B012317643

Ethical Statement

Tüm yazarlar bu çalışmaya eşit katkıda bulunmuşlardır. Makalenin son halini okuyup onaylamışlar ve metin, şekil ve tabloların özgün olduğunu ve daha önce yayınlanmadığını teyit etmişlerdir.

Thanks

Araştırmamıza destek veren TÜBİTAK'a teşekkür ederiz.

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Details

Primary Language

English

Subjects

Microbiology (Other)

Journal Section

Research Article

Authors

Aysel Veyisoğlu ^*
0000-0002-1406-5513
Türkiye

Medine Karabörk
0009-0003-7075-0798
Türkiye

Demet Tatar
0000-0002-9317-3263
Türkiye

Hünkar Avni Duyar
0000-0002-2560-5407
Türkiye

Early Pub Date

April 11, 2026

Publication Date

-

Submission Date

November 24, 2025

Acceptance Date

February 22, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1829240

IZ

https://izlik.org/JA38PX97SD

Cite

RIS / Bibtex

APA

Veyisoğlu, A., Karabörk, M., Tatar, D., & Duyar, H. A. (2026). Determination of Chemical Composition and In Vitro Antimicrobial Activity of Thymus longicaulis subsp. longicaulis Plant Extracts and Essential Oil. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication, 1380-1391. https://doi.org/10.18016/ksutarimdoga.vi.1829240