In Vitro Culture Performance of Allium scorodoprasum L. subsp. rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions

Saliha Erdoğdu; Aysun Çelik; Naif Geboloğlu

doi:10.18016/ksutarimdoga.vi.1877846

TR EN

Allium scorodoprasum L. subsp. rotundum'un İn Vitro Kültür Performansı: Eksplant tipi, Bitki Büyüme Düzenleyicileri ve Kültür Koşullarının Etkileri

Abstract

Bu çalışma, Allium scorodoprasum L. subsp. rotundum için bir mikro çoğaltım protokolü geliştirmek amacıyla yürütülmüştür. Eksplant olarak bitkinin soğanları, olgun tohumları, kökleri ve yaprakları kullanılmıştır. Eksplantlar 2 kültür ortamı (MS ve B5), 12 hormon kombinasyonu (2,4-D: 0.1, 1.0 ve 2.0 mg L⁻¹; kinetin: 0.1, 1.0 mg L⁻¹) ve iki farklı iklim ortamı (15 gün boyunca 25±2 °C sıcaklık ve sürekli karanlık, 25±2 °C sıcaklık, 16/8 saat aydınlık/karanlık ve 3000 lüks ışık yoğunluğu) altında kültüre alınmıştır. Çalışma sonucunda, yaprak eksplantlarından rejenerasyon elde edilememiştir; 8 haftalık inkübasyondan sonra yapraklar koyulaşarak kurumuştur. Kök eksplantlarında kallus oluşumu ise belirli kombinasyonlarda sağlanmıştır. Hormon kombinasyonları açısından en etkili kombinasyon B5 + 0.1 mg L⁻¹ 2,4-D + 0.1 mg L⁻¹ kinetin olmuştur. Karanlık koşullar ve B5 ortamı, MS ortamı ile karşılaştırıldığında kallus oluşumunda istatistiksel olarak önemli düzeyde bir artış sağlamıştır (P≤0.01). Ancak oluşan kalluslar bitkiye dönüşmeden koyulaşmıştır. Bulgular, A. scorodoprasum L. subsp. rotundum’un in vitro çoğaltımı için mevcut protokolün yeterli olmadığını, hormon dozu, besin ortamı ve kültür koşulları üzerinde daha fazla optimizasyon çalışması yapılması gerektiğini göstermektedir. Bu bulgular ayrıca alttürün in vitro çoğaltımı için temel bir protokolün geliştirilmesine katkıda bulunmakta ve gelecekteki in vitro çalışmalar için yol gösterici olmaktadır.Bu çalışma, Allium scorodoprasum L. subsp. rotundum için bir mikro çoğaltım protokolü geliştirmek amacıyla yürütülmüştür. Eksplant olarak soğan ve olgun tohumlardan elde edilen bitkiciklerin kök ve yaprak dokuları kullanılmıştır. Eksplantlar iki kültür ortamı (MS ve B5), on iki hormon kombinasyonu (2,4-D: 0.1, 1.0 ve 2.0 mg L⁻¹; kinetin: 0.1, 1.0 mg L⁻¹) ve iki farklı kültür koşulu (15 gün boyunca 25 ± 2 °C sıcaklık ve sürekli karanlık, 25 ± 2 °C sıcaklık, 16/8 saat aydınlık/karanlık ve 3000 lüks ışık yoğunluğu) altında kültüre alınmıştır. Yaprak eksplantlarından rejenerasyon elde edilememiştir; 8 haftalık inkübasyondan sonra yapraklar koyulaşarak kurumuştur. Kök eksplantlarında kallus oluşumu ise belirli kombinasyonlarda sağlanmıştır. Hormon kombinasyonları açısından en etkili ortam 0.1 mg L⁻¹ 2,4-D ve 0.1 mg L⁻¹ kinetin içeren B5 ortamı olmuştur. Karanlık koşullar ve B5 ortamı, MS ortamına kıyasla kallus oluşumunda önemli bir artış sağlamıştır (P≤0.01). Ancak oluşan kalluslar bitkiye dönüşmeden koyulaşmıştır. Bulgular, A. scorodoprasum L. subsp. rotundum’un in vitro çoğaltımı için mevcut protokolün yeterli olmadığını, hormon kombinasyonları, konsantrasyonları, besin ortamı ve kültür koşulları üzerinde daha fazla optimizasyon çalışması yapılması gerektiğini göstermektedir. Bu bulgular aynı zamanda alt türün in vitro çoğaltımı için temel bir protokolün geliştirilmesine katkıda bulunmaktadır ve gelecekteki in vitro çalışmaların planlanması için yol gösterici niteliktedir.

Keywords

In Vitro Culture Performance of Allium scorodoprasum L. subsp. rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions

Abstract

This study was conducted to develop a micropropagation protocol for Allium scorodoprasum L. subsp. rotundum. Root and leaf tissues of plantlets derived from bulbs and mature seeds were used as the explant. The explants were tested on two culture media (MS and B5), twelve hormone combinations (2,4-D: 0.1, 1.0, and 2.0 mg L⁻¹; kinetin: 0.1, 1.0 mg L⁻¹), and two different culture conditions (25 ± 2 °C temperature and continuous darkness for 15 days, 25 ± 2 °C temperature, 16/8 hours light/dark, and 3000 lx light intensity). Regeneration could not be obtained from the leaf explants; after 8 weeks of culture, the leaves darkened and dried out. Callus formation in root explants was achieved in certain combinations. In terms of hormone combinations, the most effective was B5 medium containing 0.1 mg L⁻¹ 2,4-D and 0.1 mg L⁻¹ kinetin. Dark conditions, and the B5 medium provided a significant increase in callus formation compared to the MS medium (P≤0.01). However, the formed calluses darkened without transforming into plants. The findings indicate that the current protocol is insufficient for the in vitro propagation of A. scorodoprasum L. subsp. rotundum and that further optimization works are needed on hormone combinations, concentrations, culture medium, and culture conditions. These findings also contribute to the development of a basic protocol for the in vitro propagation of the subspecies and provide guidance for planning future in vitro studies.

Keywords

References

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Details

Primary Language

English

Subjects

Plant Biotechnology, Green-House Growing and Treatment, Horticultural Production (Other)

Journal Section

Research Article

Authors

Saliha Erdoğdu ^*
0000-0001-8639-2938
Türkiye

Aysun Çelik
0000-0001-5289-2176
Türkiye

Naif Geboloğlu
0000-0003-2495-7088
Türkiye

Early Pub Date

November 7, 2026

Publication Date

-

Submission Date

January 30, 2026

Acceptance Date

June 29, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1877846

IZ

https://izlik.org/JA95MJ39EN

Cite

RIS / Bibtex

APA

Erdoğdu, S., Çelik, A., & Geboloğlu, N. (2026). In Vitro Culture Performance of Allium scorodoprasum L. subsp. rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1877846

AMA

1.Erdoğdu S, Çelik A, Geboloğlu N. In Vitro Culture Performance of Allium scorodoprasum L. subsp. rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions. KSU J. Agric Nat. 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1877846

Chicago

Erdoğdu, Saliha, Aysun Çelik, and Naif Geboloğlu. 2026. “In Vitro Culture Performance of Allium Scorodoprasum L. Subsp. Rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1877846.

EndNote

Erdoğdu S, Çelik A, Geboloğlu N (November 7, 2026) In Vitro Culture Performance of Allium scorodoprasum L. subsp. rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication

IEEE

[1]S. Erdoğdu, A. Çelik, and N. Geboloğlu, “In Vitro Culture Performance of Allium scorodoprasum L. subsp. rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions”, KSU J. Agric Nat., no. Advanced Online Publication, Nov. 2026, doi: 10.18016/ksutarimdoga.vi.1877846.

ISNAD

Erdoğdu, Saliha - Çelik, Aysun - Geboloğlu, Naif. “In Vitro Culture Performance of Allium Scorodoprasum L. Subsp. Rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (November 7, 2026). https://doi.org/10.18016/ksutarimdoga.vi.1877846.

JAMA

1.Erdoğdu S, Çelik A, Geboloğlu N. In Vitro Culture Performance of Allium scorodoprasum L. subsp. rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions. KSU J. Agric Nat. 2026. doi:10.18016/ksutarimdoga.vi.1877846.

MLA

Erdoğdu, Saliha, et al. “In Vitro Culture Performance of Allium Scorodoprasum L. Subsp. Rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Nov. 2026, doi:10.18016/ksutarimdoga.vi.1877846.

Vancouver

1.Saliha Erdoğdu, Aysun Çelik, Naif Geboloğlu. In Vitro Culture Performance of Allium scorodoprasum L. subsp. rotundum: Effects of Explant Type, Plant Growth Regulators, and Culture Conditions. KSU J. Agric Nat. 2026 Nov. 7;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1877846