Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)

H. Nilüfer Yıldız; Mine Özkil; Soner Soylu; İlhan Üremiş

doi:10.18016/ksutarimdoga.vi.1881665

TR EN

Semiz Otu (Portulaca oleracea L) ve Tarla Sarmaşığı (Convolvulus arvensis L.)’na Karşı Endofitik Bakterilerin Biyoherbisidal Potansiyellerinin Belirlenmesi

Abstract

Bitkisel üretimde verim ve kalite kayıplarına yol açan yabancı otları yönetmek için çevre dostu biyolojik mücadele stratejilerinin kullanılması, sürdürülebilir tarım uygulamalarıyla oldukça uyumludur. Son zamanlarda yapılan çalışmalar, belirli endofitik veya epifitik bakteriler yabancı ot tohumlarının çimlenmesini ve bitki büyümesini engelleyebileceğini göstermiştir. Bu çalışma, yabancı ot kontrolünde potansiyel Biyolojik Mücadele Ajanları (BCAs) olarak değerlendirilebilecek endofitik bakterilerin izolasyonu ve önemli yabancı ot türlerinden semizotu (Portulaca oleracea L.) ve tarla sarmaşığı (Convolvulus arvensis L.) tohumlarının çimlenmesi ile bitki büyümesi üzerindeki biyoherbisidal etkinliğinin belirlemesini amaçlamıştır. Bu kapsamda, zayıf gelişim gösteren P. oleracea ve C. arvensis bitkilerinden elde edilen, çimlenme yeteneği olmayan tohumlardan farklı morfolojik özelliklere sahip dokuz adet BCA bakteri izolatı elde edilmiştir. İzolasyonlar sonucunda yedi izolat Gram-pozitif, iki izolat ise Gram-negatif olarak tanımlanmıştır. Tütün yapraklarında aşırı duyarlılık reaksiyonu (hipersensitivite), patates dilimlerinde yumuşak çürüklük ve 37 °C’de büyüme testlerinde negatif sonuç veren dört izolat (P1, P5, C1 ve C3), MALDI-TOF MS yöntemiyle sırasıyla Pseudarthrobacter oxydans, Paenibacillus polymyxa, Curtobacterium flaccumfaciens pv. flaccumfaciens ve Bacillus megaterium olarak tanılanmıştır. C. arvensis tohumlarından izole edilen C. flaccumfaciens pv. flaccumfaciens C1 izolatı, fasulye bitkilerinde yapılan patojenite testlerinde tipik hastalık semptomlarına neden olmuştur. Bu çalışma, C. arvensis’in C. flaccumfaciens pv. flaccumfaciens için alternatif bir konukçu olduğunu ortaya koymuştur. Saksı denemelerinde, P. oxydans P1 ve P. polymyxa P5, sağlıklı P. oleracea tohumlarının çimlenmesini sırasıyla %76 ve %85 oranında engellerken, C. flaccumfaciens pv. flaccumfaciens C1 ve B. megaterium C3, kontrol grubuna kıyasla sağlıklı C. arvensis tohumlarının çimlenmesini sırasıyla %51.7 ve %58.6 oranında engellemiştir. Etkili BCA izolatlarının mısır, pamuk veya havuç tohumlarının çimlenmesi üzerinde herhangi bir olumsuz etkisi tespit edilmemiştir. Elde edilen sonuçlar, yabancı ot tohumlarının çimlenmesini etkili şekilde engelleyen bakteri izolatlarının, yabancı otların biyolojik kontrolünde potansiyel biyoherbisit adayları olabileceğini göstermiştir.

Keywords

Supporting Institution

Biyolojik Mücadele Araştırma Enstitüsü

Project Number

The data used in this publication was obtained as a preliminary study, not as part of the project.

Ethical Statement

Bu makalede, yazarların hiçbiri tarafından insan veya hayvan katılımcılarla gerçekleştirilen herhangi bir çalışma bulunmamaktadır. Bu nedenle, bu çalışma etik kurul onayı gerektirmemiştir.

Thanks

Yazarlar, bu çalışmanın yürütülmesindeki desteklerinden dolayı Türkiye Cumhuriyeti Tarım ve Orman Bakanlığı Tarımsal Araştırma ve Politikalar Genel Müdürlüğü'ne (TAGEM) teşekkürlerini sunarlar.

Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)

Abstract

The use of environmentally friendly biological control strategies to manage weeds that cause yield and quality losses in plant production is highly compatible with sustainable agricultural practices. Recent studies have demonstrated that certain endophytic or epiphytic bacteria can suppress weed germination and growth. This study aimed to isolate endophytic bacteria that could be evaluated as putative Biological Control Agents (BCAs) for weed control and to determine their bioherbicidal activity. In this context, nine dominant BCA bacterial isolates with district morphological characteristics were obtained from the seeds of common purslane (Portulaca oleracea L.) and field bindweed (Convolvulus arvensis, L.) which are important weed species exhibiting stunted growth or poor vigour in the field. Seven isolates were identified as Gram positive and two as Gram negative. Four isolates, that gave negative results in tobacco hypersensitivity reaction, potato soft rot, and growth tests at 37 °C, were identified as Pseudarthrobacter oxydans P1, Paenibacillus polymyxa P5, Curtobacterium flaccumfaciens pv. flaccumfaciens C1, and Bacillus megaterium C3 using MALDI TOF MS method. The C. flaccumfaciens pv. flaccumfaciens C1 isolate, originating from C. arvensis, induced typical disease symptoms in pathogenicity tests on bean plants. This study demonstrates for the first time that C. arvensis is an alternative host for C. flaccumfaciens pv. flaccumfaciens. In pot experiments, P. oxydans P1 and P. polymyxa P5 inhibited the germination of P. oleracea seeds by 76% and 85%, respectively, while C. flaccumfaciens pv. flaccumfaciens C1 and B. megaterium C3 reduced the germination of C. arvensis seeds by 51.7% and 58.6%, respectively, compared to the control. Furthermore, no adverse effects were observed on the germination of maize, cotton, or carrot seeds. These findings indicate that the selected bacterial isolates may be potential bioherbicide candidates in biological weed control by inhibiting the germination of weed seeds.

Keywords

Supporting Institution

Biological Control Research Institute

Project Number

The data used in this publication was obtained as a preliminary study, not as part of the project.

Ethical Statement

This article lacks any study related to human or animal participants performed by any of the authors. Therefore, this study did not require ethical approval.

Thanks

Authors would like to thank the Republic of Türkiye Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies (TAGEM) for its support in carrying out this study.

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Details

Primary Language

English

Subjects

Phytopathology, Herbology

Journal Section

Research Article

Authors

H. Nilüfer Yıldız ^*
0000-0002-1945-2272
Türkiye

Mine Özkil
2000-0000-2766-3736
Türkiye

Soner Soylu
0000-0003-1002-8958
Türkiye

İlhan Üremiş
0000-0001-5937-9244
Türkiye

Early Pub Date

November 7, 2026

Publication Date

-

Submission Date

February 4, 2026

Acceptance Date

June 16, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1881665

IZ

https://izlik.org/JA97SL98EW

Cite

RIS / Bibtex

APA

Yıldız, H. N., Özkil, M., Soylu, S., & Üremiş, İ. (2026). Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1881665

AMA

1.Yıldız HN, Özkil M, Soylu S, Üremiş İ. Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). KSU J. Agric Nat. 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1881665

Chicago

Yıldız, H. Nilüfer, Mine Özkil, Soner Soylu, and İlhan Üremiş. 2026. “Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca Oleracea L.) and Field Bindweed (Convolvulus Arvensis L.)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1881665.

EndNote

Yıldız HN, Özkil M, Soylu S, Üremiş İ (November 7, 2026) Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication

IEEE

[1]H. N. Yıldız, M. Özkil, S. Soylu, and İ. Üremiş, “Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)”, KSU J. Agric Nat., no. Advanced Online Publication, Nov. 2026, doi: 10.18016/ksutarimdoga.vi.1881665.

ISNAD

Yıldız, H. Nilüfer - Özkil, Mine - Soylu, Soner - Üremiş, İlhan. “Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca Oleracea L.) and Field Bindweed (Convolvulus Arvensis L.)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (November 7, 2026). https://doi.org/10.18016/ksutarimdoga.vi.1881665.

JAMA

1.Yıldız HN, Özkil M, Soylu S, Üremiş İ. Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). KSU J. Agric Nat. 2026. doi:10.18016/ksutarimdoga.vi.1881665.

MLA

Yıldız, H. Nilüfer, et al. “Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca Oleracea L.) and Field Bindweed (Convolvulus Arvensis L.)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Nov. 2026, doi:10.18016/ksutarimdoga.vi.1881665.

Vancouver

1.H. Nilüfer Yıldız, Mine Özkil, Soner Soylu, İlhan Üremiş. Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). KSU J. Agric Nat. 2026 Nov. 7;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1881665