Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)

H. Nilüfer Yıldız; Mine Özkil; Soner Soylu; İlhan Üremiş

doi:10.18016/ksutarimdoga.vi.1881665

TR EN

Semiz Otu (Portulaca oleracea L) ve Tarla Sarmaşığı (Convolvulus arvensis L.)’na Karşı Endofitik Bakterilerin Biyoherbisidal Potansiyellerinin Belirlenmesi

Öz

Bitkisel üretimde verim ve kalite kayıplarına yol açan yabancı otları yönetmek için çevre dostu biyolojik mücadele stratejilerinin kullanılması, sürdürülebilir tarım uygulamalarıyla oldukça uyumludur. Son zamanlarda yapılan çalışmalar, belirli endofitik veya epifitik bakteriler yabancı ot tohumlarının çimlenmesini ve bitki büyümesini engelleyebileceğini göstermiştir. Bu çalışma, yabancı ot kontrolünde potansiyel Biyolojik Mücadele Ajanları (BCAs) olarak değerlendirilebilecek endofitik bakterilerin izolasyonu ve önemli yabancı ot türlerinden semizotu (Portulaca oleracea L.) ve tarla sarmaşığı (Convolvulus arvensis L.) tohumlarının çimlenmesi ile bitki büyümesi üzerindeki biyoherbisidal etkinliğinin belirlemesini amaçlamıştır. Bu kapsamda, zayıf gelişim gösteren P. oleracea ve C. arvensis bitkilerinden elde edilen, çimlenme yeteneği olmayan tohumlardan farklı morfolojik özelliklere sahip dokuz adet BCA bakteri izolatı elde edilmiştir. İzolasyonlar sonucunda yedi izolat Gram-pozitif, iki izolat ise Gram-negatif olarak tanımlanmıştır. Tütün yapraklarında aşırı duyarlılık reaksiyonu (hipersensitivite), patates dilimlerinde yumuşak çürüklük ve 37 °C’de büyüme testlerinde negatif sonuç veren dört izolat (P1, P5, C1 ve C3), MALDI-TOF MS yöntemiyle sırasıyla Pseudarthrobacter oxydans, Paenibacillus polymyxa, Curtobacterium flaccumfaciens pv. flaccumfaciens ve Bacillus megaterium olarak tanılanmıştır. C. arvensis tohumlarından izole edilen C. flaccumfaciens pv. flaccumfaciens C1 izolatı, fasulye bitkilerinde yapılan patojenite testlerinde tipik hastalık semptomlarına neden olmuştur. Bu çalışma, C. arvensis’in C. flaccumfaciens pv. flaccumfaciens için alternatif bir konukçu olduğunu ortaya koymuştur. Saksı denemelerinde, P. oxydans P1 ve P. polymyxa P5, sağlıklı P. oleracea tohumlarının çimlenmesini sırasıyla %76 ve %85 oranında engellerken, C. flaccumfaciens pv. flaccumfaciens C1 ve B. megaterium C3, kontrol grubuna kıyasla sağlıklı C. arvensis tohumlarının çimlenmesini sırasıyla %51.7 ve %58.6 oranında engellemiştir. Etkili BCA izolatlarının mısır, pamuk veya havuç tohumlarının çimlenmesi üzerinde herhangi bir olumsuz etkisi tespit edilmemiştir. Elde edilen sonuçlar, yabancı ot tohumlarının çimlenmesini etkili şekilde engelleyen bakteri izolatlarının, yabancı otların biyolojik kontrolünde potansiyel biyoherbisit adayları olabileceğini göstermiştir.

Anahtar Kelimeler

Destekleyen Kurum

Biyolojik Mücadele Araştırma Enstitüsü

Proje Numarası

The data used in this publication was obtained as a preliminary study, not as part of the project.

Etik Beyan

Bu makalede, yazarların hiçbiri tarafından insan veya hayvan katılımcılarla gerçekleştirilen herhangi bir çalışma bulunmamaktadır. Bu nedenle, bu çalışma etik kurul onayı gerektirmemiştir.

Teşekkür

Yazarlar, bu çalışmanın yürütülmesindeki desteklerinden dolayı Türkiye Cumhuriyeti Tarım ve Orman Bakanlığı Tarımsal Araştırma ve Politikalar Genel Müdürlüğü'ne (TAGEM) teşekkürlerini sunarlar.

Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)

Öz

The use of environmentally friendly biological control strategies to manage weeds that cause yield and quality losses in plant production is highly compatible with sustainable agricultural practices. Recent studies have demonstrated that certain endophytic or epiphytic bacteria can suppress weed germination and growth. This study aimed to isolate endophytic bacteria that could be evaluated as putative Biological Control Agents (BCAs) for weed control and to determine their bioherbicidal activity. In this context, nine dominant BCA bacterial isolates with district morphological characteristics were obtained from the seeds of common purslane (Portulaca oleracea L.) and field bindweed (Convolvulus arvensis, L.) which are important weed species exhibiting stunted growth or poor vigour in the field. Seven isolates were identified as Gram positive and two as Gram negative. Four isolates, that gave negative results in tobacco hypersensitivity reaction, potato soft rot, and growth tests at 37 °C, were identified as Pseudarthrobacter oxydans P1, Paenibacillus polymyxa P5, Curtobacterium flaccumfaciens pv. flaccumfaciens C1, and Bacillus megaterium C3 using MALDI TOF MS method. The C. flaccumfaciens pv. flaccumfaciens C1 isolate, originating from C. arvensis, induced typical disease symptoms in pathogenicity tests on bean plants. This study demonstrates for the first time that C. arvensis is an alternative host for C. flaccumfaciens pv. flaccumfaciens. In pot experiments, P. oxydans P1 and P. polymyxa P5 inhibited the germination of P. oleracea seeds by 76% and 85%, respectively, while C. flaccumfaciens pv. flaccumfaciens C1 and B. megaterium C3 reduced the germination of C. arvensis seeds by 51.7% and 58.6%, respectively, compared to the control. Furthermore, no adverse effects were observed on the germination of maize, cotton, or carrot seeds. These findings indicate that the selected bacterial isolates may be potential bioherbicide candidates in biological weed control by inhibiting the germination of weed seeds.

Anahtar Kelimeler

Destekleyen Kurum

Biological Control Research Institute

Proje Numarası

The data used in this publication was obtained as a preliminary study, not as part of the project.

Etik Beyan

This article lacks any study related to human or animal participants performed by any of the authors. Therefore, this study did not require ethical approval.

Teşekkür

Authors would like to thank the Republic of Türkiye Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies (TAGEM) for its support in carrying out this study.

Kaynakça

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Soylu, E.M., Soylu, S., Kara, M. & Kurt, Ş. (2020). Sebzelerde sorun olan önemli bitki fungal hastalık etmenlerine karşı vermikomposttan izole edilen mikrobiyomların in vitro antagonistik etkilerinin belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23, 7-18. https://doi.org/10.18016/ksutarimdoga.vi.601936
Soylu, S., Kara, M., Soylu, E. M., Uysal, A. & Kurt, Ş. (2022). Geotrichum citri-aurantii’nin sebep olduğu turunçgil ekşi çürüklük hastalığının biyolojik mücadelesinde endofit bakterilerin biyokontrol potansiyellerinin belirlenmesi. Tekirdağ Ziraat Fakültesi Dergisi, 19(1), 177-191. https://doi.org/10.33462/jotaf.944704
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Ayrıntılar

Birincil Dil

İngilizce

Konular

Fitopatoloji, Herboloji

Bölüm

Araştırma Makalesi

Yazarlar

H. Nilüfer Yıldız ^*
0000-0002-1945-2272
Türkiye

Mine Özkil
2000-0000-2766-3736
Türkiye

Soner Soylu
0000-0003-1002-8958
Türkiye

İlhan Üremiş
0000-0001-5937-9244
Türkiye

Erken Görünüm Tarihi

7 Kasım 2026

Yayımlanma Tarihi

-

Gönderilme Tarihi

4 Şubat 2026

Kabul Tarihi

16 Haziran 2026

Yayımlandığı Sayı

Yıl 2026 Sayı: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1881665

IZ

https://izlik.org/JA97SL98EW

Kaynak Göster

RIS / Bibtex

APA

Yıldız, H. N., Özkil, M., Soylu, S., & Üremiş, İ. (2026). Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1881665

AMA

1.Yıldız HN, Özkil M, Soylu S, Üremiş İ. Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1881665

Chicago

Yıldız, H. Nilüfer, Mine Özkil, Soner Soylu, ve İlhan Üremiş. 2026. “Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1881665.

EndNote

Yıldız HN, Özkil M, Soylu S, Üremiş İ (07 Kasım 2026) Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication

IEEE

[1]H. N. Yıldız, M. Özkil, S. Soylu, ve İ. Üremiş, “Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)”, Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication, Kas. 2026, doi: 10.18016/ksutarimdoga.vi.1881665.

ISNAD

Yıldız, H. Nilüfer - Özkil, Mine - Soylu, Soner - Üremiş, İlhan. “Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (07 Kasım 2026). https://doi.org/10.18016/ksutarimdoga.vi.1881665.

JAMA

1.Yıldız HN, Özkil M, Soylu S, Üremiş İ. Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 2026. doi:10.18016/ksutarimdoga.vi.1881665.

MLA

Yıldız, H. Nilüfer, vd. “Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, sy Advanced Online Publication, Kasım 2026, doi:10.18016/ksutarimdoga.vi.1881665.

Vancouver

1.H. Nilüfer Yıldız, Mine Özkil, Soner Soylu, İlhan Üremiş. Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. 07 Kasım 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1881665

Semiz Otu (Portulaca oleracea L) ve Tarla Sarmaşığı (Convolvulus arvensis L.)’na Karşı Endofitik Bakterilerin Biyoherbisidal Potansiyellerinin Belirlenmesi

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Etik Beyan

Teşekkür

Determination of the Bioherbicidal Potential of the Endophytic Bacterial Isolates Against Common Purslane (Portulaca oleracea L.) and Field Bindweed (Convolvulus arvensis L.)

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Etik Beyan

Teşekkür

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Ayrıntılar

Birincil Dil

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Bölüm

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DOI

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e-ISSN: 2619-9149