Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye

Bilal Yakut; Ramazan Çetintaş; Tolga Gürkan; Betül Gürkan

doi:10.18016/ksutarimdoga.vi.1910433

TR EN

Diyarbakır İli Hevsel Bahçeleri Sebze Alanlarında Kök-ur Nematodlarının (Meloidogyne spp.) Tespiti ve Yayılımının Belirlenmesi

Abstract

Hevsel Bahçeleri (Diyarbakır, Türkiye) sebze üretim alanlarında kök-ur nematodlarının varlığı ve yayılışı, 2019-2020 yıllarında yürütülen sürvey çalışmasıyla belirlenmiştir. Çalışmada 50 sebze üretim parseli incelenmiş, her parselden 5-10 bitkiye ait kök örneği alınmış ve toplam 455 bitki örneği değerlendirilmiştir. Urlu kökler kök-ur skalasına göre değerlendirilmiştir. Tür teşhisi, dişi bireylerden hazırlanan perineal pattern (anal kesit) preparatlarının ışık mikroskobu altında incelenmesi ve tanı anahtarlarıyla karşılaştırılması esas alınarak yapılmıştır. Morfolojik teşhisi desteklemek amacıyla esteraz izozim fenotiplemesi (EST-PAGE) uygulanmış ve bant paternleri referans fenotiplerle karşılaştırılmıştır. Her iki yöntem sonucunda kök-ur nematodlarının Meloidogyne javanica, M. incognita ve M. arenaria türlerine ait olduğu belirlenmiştir. EST-PAGE ile doğrulanan 22 popülasyon esas alındığında, türlerin yaygınlık oranları sırasıyla %63,6, %22,7 ve %13,6 olarak hesaplanmıştır. İncelenen 50 parselin 22’sinin (%44,0) kök-ur nematodları ile bulaşık olduğu saptanmıştır. Bulaşık parsellerden alınan 219 bitki örneğinin 203’ünde kök-ur nematodu tespit edilmiş ve bu parsellerde bitki örneği düzeyindeki bulaşıklık oranı %92,7 olarak hesaplanmıştır.

Keywords

Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye

Abstract

A survey conducted in vegetable production areas of the Hevsel Gardens (Diyarbakır, Türkiye) during 2019-2020 determined the occurrence and distribution of root-knot nematodes (Meloidogyne spp.). Fifty vegetable production plots were surveyed, 5-10 plants root samples were collected from each plot, and a total of 455 plant samples were evaluated. Galled roots were assessed using a root-galling scale. Species identification was based on the examination of perineal pattern preparations obtained from females under a light microscope and comparison with diagnostic keys. To support morphological identification, esterase isozyme phenotype (EST-PAGE) was performed, and banding patterns were compared with reference phenotypes. Both methods identified the detected root-knot nematodes as Meloidogyne javanica, M. incognita, and M. arenaria. Based on the 22 PAGE-confirmed populations, the prevalence rates of these species were 63.6%, 22.7%, and 13.6%, respectively. Of the 50 plots examined, 22 (44.0%) were infested with root-knot nematodes. Root-knot nematodes were detected in 203 of the 219 plant samples collected from the infested plots, representing a plant-sample infestation rate of 92.7% within infested plots.

Keywords

References

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Details

Primary Language

English

Subjects

Nematology

Journal Section

Research Article

Authors

Bilal Yakut
0000-0002-6414-1457
Türkiye

Ramazan Çetintaş
0000-0002-5738-6915
Türkiye

Tolga Gürkan ^*
0000-0003-0839-6559
Türkiye

Betül Gürkan
0000-0003-0195-4562
Türkiye

Early Pub Date

November 7, 2026

Publication Date

-

Submission Date

March 16, 2026

Acceptance Date

June 2, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1910433

IZ

https://izlik.org/JA49RS43ZR

Cite

RIS / Bibtex

APA

Yakut, B., Çetintaş, R., Gürkan, T., & Gürkan, B. (2026). Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1910433

AMA

1.Yakut B, Çetintaş R, Gürkan T, Gürkan B. Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. KSU J. Agric Nat. 2026;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1910433

Chicago

Yakut, Bilal, Ramazan Çetintaş, Tolga Gürkan, and Betül Gürkan. 2026. “Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne Spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication. https://doi.org/10.18016/ksutarimdoga.vi.1910433.

EndNote

Yakut B, Çetintaş R, Gürkan T, Gürkan B (November 7, 2026) Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Advanced Online Publication

IEEE

[1]B. Yakut, R. Çetintaş, T. Gürkan, and B. Gürkan, “Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye”, KSU J. Agric Nat., no. Advanced Online Publication, Nov. 2026, doi: 10.18016/ksutarimdoga.vi.1910433.

ISNAD

Yakut, Bilal - Çetintaş, Ramazan - Gürkan, Tolga - Gürkan, Betül. “Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne Spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi. Advanced Online Publication (November 7, 2026). https://doi.org/10.18016/ksutarimdoga.vi.1910433.

JAMA

1.Yakut B, Çetintaş R, Gürkan T, Gürkan B. Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. KSU J. Agric Nat. 2026. doi:10.18016/ksutarimdoga.vi.1910433.

MLA

Yakut, Bilal, et al. “Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne Spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, no. Advanced Online Publication, Nov. 2026, doi:10.18016/ksutarimdoga.vi.1910433.

Vancouver

1.Bilal Yakut, Ramazan Çetintaş, Tolga Gürkan, Betül Gürkan. Occurrence and Distribution of Root-Knot Nematodes (Meloidogyne spp.) in Vegetable Fields of the Hevsel Gardens, Diyarbakır Province, Türkiye. KSU J. Agric Nat. 2026 Nov. 7;(Advanced Online Publication). doi:10.18016/ksutarimdoga.vi.1910433